BrmeA.00107.b

RND family efflux transporter, MFP subunit

CENTER ID: BrmeA.00107.b
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00107.b.B2.GE36676 Mature protein( BrmeA.00107.b ) 22 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00107.b.B2.PS01819 Mature protein( BrmeA.00107.b ) 22 360
BrmeA.00107.b.B2.PS01820 Mature protein( BrmeA.00107.b ) 22 360

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_2685
BV-BRC: fig|224914.11.peg.2685
RefSeq: NP_541358.1
UniProt: Q8YCZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGAFAGLATC IQILSAGSPA YADSLNVVPV AVREAKLVDY QPKLTLSGAV AARSLVNVSF RVNGQVVERL VDIGQHVEKG QLLARIDDVE QRANMRVAQA SMDAAEAQLV QADANFKRQQ ALLKQGFTTR SQYDQADQAL RTAQSALSSA QSQLGNARDE LSYTELHAPV SGVVTARNIE TGQVVQAAQT AFSIAEDGSR DAIFDVQETL VNHARIGMGV TVALLADPSI EAEGNIRELS PVVDAQTGTV RVKVGIAQAT AQMALGAIVT GTVHMDARQV IVLPWSAMAS EAGRTAVWRV DRDTHVATLV PIKVLAYEKE RVIVESGLNN GELIVTKGAQ MLRSGEPVEI MQGPEGAGSQ
NT Sequence
TTGGGCGCAT TCGCCGGTCT TGCAACCTGC ATTCAGATCC TGTCGGCCGG AAGTCCGGCA TATGCTGATT CACTCAATGT CGTGCCGGTT GCGGTCCGGG AAGCGAAGCT TGTCGATTAC CAGCCGAAAT TGACGCTTTC CGGGGCTGTC GCCGCGCGCT CTCTGGTCAA TGTATCGTTC CGCGTCAATG GTCAGGTGGT CGAACGGCTG GTCGATATCG GGCAGCATGT CGAAAAGGGG CAGCTTCTGG CCCGCATCGA CGATGTTGAA CAGCGCGCCA ATATGCGCGT TGCGCAGGCT TCCATGGACG CCGCAGAAGC GCAGCTCGTG CAGGCCGACG CCAATTTCAA GCGCCAGCAG GCGCTGTTGA AGCAGGGTTT TACCACGCGC AGCCAGTATG ACCAGGCCGA TCAGGCATTG CGCACCGCGC AAAGTGCGCT TTCCAGCGCG CAAAGCCAGC TTGGCAATGC ACGCGACGAG CTTTCCTATA CCGAGCTTCA TGCCCCTGTT TCAGGCGTCG TTACGGCGCG CAATATCGAA ACGGGTCAGG TCGTGCAGGC AGCGCAAACG GCTTTCTCCA TTGCGGAAGA CGGCTCGCGC GACGCTATTT TCGATGTGCA GGAAACATTG GTGAATCATG CCCGCATTGG AATGGGCGTA ACGGTTGCCC TGCTTGCCGA TCCTTCCATT GAAGCAGAGG GCAATATCCG TGAATTGTCG CCGGTTGTTG ACGCACAGAC CGGCACAGTG CGGGTCAAGG TAGGCATTGC GCAGGCAACT GCGCAGATGG CGCTTGGCGC AATCGTCACC GGAACTGTCC ATATGGATGC GCGGCAGGTG ATCGTGCTGC CATGGAGCGC GATGGCCTCG GAGGCGGGAC GAACGGCCGT CTGGCGCGTG GATAGGGACA CGCATGTCGC GACACTCGTT CCGATCAAGG TGCTGGCCTA TGAAAAAGAG CGCGTGATCG TCGAAAGCGG CCTTAATAAC GGTGAGCTTA TCGTGACCAA AGGTGCGCAG ATGCTGCGCT CCGGCGAGCC GGTAGAGATC ATGCAGGGGC CTGAAGGAGC TGGCTCGCAA
Details for BrmeA.00107.b.B2.GE36676
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Validated AA Sequence
MRVAQASMDA AEAQLVQADA NFKRQQALLK QGFTTRSQYD QADQALRTAQ SALSSAQSQL GNARDELSYT ELHAPVSGVV TARNIETGQV VQAAQTAFSI AEDGSRDAIF DVQETLVNHA RIGMGVTVAL LADPSIEAEG NIRELSPVVD AQTGTVRVKV GIAQATAQMA LGAIVTGTVH MDARQVIVLP WSAMASEAGR TAVWRVDRDT HVATLVPIKV LAYEKERVIV ESGLNNGELI VTKGAQMLRS GEPVEIMQ
Validated NT Sequence
ccctgcatga tctctaccgg ctcgccggag cgcagcatct gcgcaccttt ggtcacgata agctcaccgt tattaaggcc gctttcgacg atcacgcgct ctttttcata ggccagcacc ttgatcggaa cgagtgtcgc gacatgcgtg tccctatcca cgcgccagac ggccgttcgt cccgcctccg aggccatcgc gctccatggc agcacgatca cctgccgcgc atccatatgg acagttccgg tgacgattgc gccaagcgcc atctgcgcag ttgcctgcgc aatgcctacc ttgacccgca ctgtgccggt ctgtgcgtca acaaccggcg acaattcacg gatattgccc tctgcttcaa tggaaggatc ggcaagcagg gcaaccgtta cgcccattcc aatgcgggca tgattcacca atgtttcctg cacatcgaaa atagcgtcgc gcgagccgtc ttccgcaatg gagaaagccg tttgcgctgc ctgcacgacc tgacccgttt cgatattgcg cgccgtaacg acgcctgaaa caggggcatg aagctcggta taggaaagct cgtcgcgtgc attgccaagc tggctttgcg cgctggaaag cgcactttgc gcggtgcgca atgcctgatc ggcctggtca tactggctgc gcgtggtaaa accctgcttc aacagcgcct gctggcgctt gaaattggcg tcggcctgca cgagctgcgc ttctgcggcg tccatggaag cctgcgcaac gcgcatattg gcgcgctgtt caacatcgtc gatgcgggcc agaagctgcc ccttttcgac atgctgcccg atatcgacca gccgttcgac cacctgacca ttgacgcgga acgatacatt gaccagagag cgcgcggcga cagccccgga aagcgtcaat ttcggctggt aatcgacaag cttcgcttcc cggaccgcaa ccggcacgac attgagtg
Expected Protein Sequence
MAHHHHHHAD SLNVVPVAVR EAKLVDYQPK LTLSGAVAAR SLVNVSFRVN GQVVERLVDI GQHVEKGQLL ARIDDVEQRA NMRVAQASMD AAEAQLVQAD ANFKRQQALL KQGFTTRSQY DQADQALRTA QSALSSAQSQ LGNARDELSY TELHAPVSGV VTARNIETGQ VVQAAQTAFS IAEDGSRDAI FDVQETLVNH ARIGMGVTVA LLADPSIEAE GNIRELSPVV DAQTGTVRVK VGIAQATAQM ALGAIVTGTV HMDARQVIVL PWSAMASEAG RTAVWRVDRD THVATLVPIK VLAYEKERVI VESGLNNGEL IVTKGAQMLR SGEPVEIMQG PEGAGSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgctgatt cactcaatgt cgtgccggtt gcggtccggg aagcgaagct tgtcgattac cagccgaaat tgacgctttc cggggctgtc gccgcgcgct ctctggtcaa tgtatcgttc cgcgtcaatg gtcaggtggt cgaacggctg gtcgatatcg ggcagcatgt cgaaaagggg cagcttctgg cccgcatcga cgatgttgaa cagcgcgcca atatgcgcgt tgcgcaggct tccatggacg ccgcagaagc gcagctcgtg caggccgacg ccaatttcaa gcgccagcag gcgctgttga agcagggttt taccacgcgc agccagtatg accaggccga tcaggcattg cgcaccgcgc aaagtgcgct ttccagcgcg caaagccagc ttggcaatgc acgcgacgag ctttcctata ccgagcttca tgcccctgtt tcaggcgtcg ttacggcgcg caatatcgaa acgggtcagg tcgtgcaggc agcgcaaacg gctttctcca ttgcggaaga cggctcgcgc gacgctattt tcgatgtgca ggaaacattg gtgaatcatg cccgcattgg aatgggcgta acggttgccc tgcttgccga tccttccatt gaagcagagg gcaatatccg tgaattgtcg ccggttgttg acgcacagac cggcacagtg cgggtcaagg taggcattgc gcaggcaact gcgcagatgg cgcttggcgc aatcgtcacc ggaactgtcc atatggatgc gcggcaggtg atcgtgctgc catggagcgc gatggcctcg gaggcgggac gaacggccgt ctggcgcgtg gatagggaca cgcatgtcgc gacactcgtt ccgatcaagg tgctggccta tgaaaaagag cgcgtgatcg tcgaaagcgg ccttaataac ggtgagctta tcgtgaccaa aggtgcgcag atgctgcgct ccggcgagcc ggtagagatc atgcaggggc ctgaaggagc tggctcgcaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BrmeA.00107.b.B2.PS01819
PURIFICATION DATe: 5/8/2013
CONCENTRATION: 21.4mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Protocol Notes
notes unavailable
Validated AA Sequence
MRVAQASMDA AEAQLVQADA NFKRQQALLK QGFTTRSQYD QADQALRTAQ SALSSAQSQL GNARDELSYT ELHAPVSGVV TARNIETGQV VQAAQTAFSI AEDGSRDAIF DVQETLVNHA RIGMGVTVAL LADPSIEAEG NIRELSPVVD AQTGTVRVKV GIAQATAQMA LGAIVTGTVH MDARQVIVLP WSAMASEAGR TAVWRVDRDT HVATLVPIKV LAYEKERVIV ESGLNNGELI VTKGAQMLRS GEPVEIMQ
Validated NT Sequence
ccctgcatga tctctaccgg ctcgccggag cgcagcatct gcgcaccttt ggtcacgata agctcaccgt tattaaggcc gctttcgacg atcacgcgct ctttttcata ggccagcacc ttgatcggaa cgagtgtcgc gacatgcgtg tccctatcca cgcgccagac ggccgttcgt cccgcctccg aggccatcgc gctccatggc agcacgatca cctgccgcgc atccatatgg acagttccgg tgacgattgc gccaagcgcc atctgcgcag ttgcctgcgc aatgcctacc ttgacccgca ctgtgccggt ctgtgcgtca acaaccggcg acaattcacg gatattgccc tctgcttcaa tggaaggatc ggcaagcagg gcaaccgtta cgcccattcc aatgcgggca tgattcacca atgtttcctg cacatcgaaa atagcgtcgc gcgagccgtc ttccgcaatg gagaaagccg tttgcgctgc ctgcacgacc tgacccgttt cgatattgcg cgccgtaacg acgcctgaaa caggggcatg aagctcggta taggaaagct cgtcgcgtgc attgccaagc tggctttgcg cgctggaaag cgcactttgc gcggtgcgca atgcctgatc ggcctggtca tactggctgc gcgtggtaaa accctgcttc aacagcgcct gctggcgctt gaaattggcg tcggcctgca cgagctgcgc ttctgcggcg tccatggaag cctgcgcaac gcgcatattg gcgcgctgtt caacatcgtc gatgcgggcc agaagctgcc ccttttcgac atgctgcccg atatcgacca gccgttcgac cacctgacca ttgacgcgga acgatacatt gaccagagag cgcgcggcga cagccccgga aagcgtcaat ttcggctggt aatcgacaag cttcgcttcc cggaccgcaa ccggcacgac attgagtg
Expressed Protein Sequence
MAHHHHHHAD SLNVVPVAVR EAKLVDYQPK LTLSGAVAAR SLVNVSFRVN GQVVERLVDI GQHVEKGQLL ARIDDVEQRA NMRVAQASMD AAEAQLVQAD ANFKRQQALL KQGFTTRSQY DQADQALRTA QSALSSAQSQ LGNARDELSY TELHAPVSGV VTARNIETGQ VVQAAQTAFS IAEDGSRDAI FDVQETLVNH ARIGMGVTVA LLADPSIEAE GNIRELSPVV DAQTGTVRVK VGIAQATAQM ALGAIVTGTV HMDARQVIVL PWSAMASEAG RTAVWRVDRD THVATLVPIK VLAYEKERVI VESGLNNGEL IVTKGAQMLR SGEPVEIMQG PEGAGSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGATT CACTCAATGT CGTGCCGGTT GCGGTCCGGG AAGCGAAGCT TGTCGATTAC CAGCCGAAAT TGACGCTTTC CGGGGCTGTC GCCGCGCGCT CTCTGGTCAA TGTATCGTTC CGCGTCAATG GTCAGGTGGT CGAACGGCTG GTCGATATCG GGCAGCATGT CGAAAAGGGG CAGCTTCTGG CCCGCATCGA CGATGTTGAA CAGCGCGCCA ATATGCGCGT TGCGCAGGCT TCCATGGACG CCGCAGAAGC GCAGCTCGTG CAGGCCGACG CCAATTTCAA GCGCCAGCAG GCGCTGTTGA AGCAGGGTTT TACCACGCGC AGCCAGTATG ACCAGGCCGA TCAGGCATTG CGCACCGCGC AAAGTGCGCT TTCCAGCGCG CAAAGCCAGC TTGGCAATGC ACGCGACGAG CTTTCCTATA CCGAGCTTCA TGCCCCTGTT TCAGGCGTCG TTACGGCGCG CAATATCGAA ACGGGTCAGG TCGTGCAGGC AGCGCAAACG GCTTTCTCCA TTGCGGAAGA CGGCTCGCGC GACGCTATTT TCGATGTGCA GGAAACATTG GTGAATCATG CCCGCATTGG AATGGGCGTA ACGGTTGCCC TGCTTGCCGA TCCTTCCATT GAAGCAGAGG GCAATATCCG TGAATTGTCG CCGGTTGTTG ACGCACAGAC CGGCACAGTG CGGGTCAAGG TAGGCATTGC GCAGGCAACT GCGCAGATGG CGCTTGGCGC AATCGTCACC GGAACTGTCC ATATGGATGC GCGGCAGGTG ATCGTGCTGC CATGGAGCGC GATGGCCTCG GAGGCGGGAC GAACGGCCGT CTGGCGCGTG GATAGGGACA CGCATGTCGC GACACTCGTT CCGATCAAGG TGCTGGCCTA TGAAAAAGAG CGCGTGATCG TCGAAAGCGG CCTTAATAAC GGTGAGCTTA TCGTGACCAA AGGTGCGCAG ATGCTGCGCT CCGGCGAGCC GGTAGAGATC ATGCAGGGGC CTGAAGGAGC TGGCTCGCAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BrmeA.00107.b.B2.PS01820
PURIFICATION DATe: 5/8/2013
CONCENTRATION: 29.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 74
Protocol Notes
notes unavailable
Validated AA Sequence
MRVAQASMDA AEAQLVQADA NFKRQQALLK QGFTTRSQYD QADQALRTAQ SALSSAQSQL GNARDELSYT ELHAPVSGVV TARNIETGQV VQAAQTAFSI AEDGSRDAIF DVQETLVNHA RIGMGVTVAL LADPSIEAEG NIRELSPVVD AQTGTVRVKV GIAQATAQMA LGAIVTGTVH MDARQVIVLP WSAMASEAGR TAVWRVDRDT HVATLVPIKV LAYEKERVIV ESGLNNGELI VTKGAQMLRS GEPVEIMQ
Validated NT Sequence
ccctgcatga tctctaccgg ctcgccggag cgcagcatct gcgcaccttt ggtcacgata agctcaccgt tattaaggcc gctttcgacg atcacgcgct ctttttcata ggccagcacc ttgatcggaa cgagtgtcgc gacatgcgtg tccctatcca cgcgccagac ggccgttcgt cccgcctccg aggccatcgc gctccatggc agcacgatca cctgccgcgc atccatatgg acagttccgg tgacgattgc gccaagcgcc atctgcgcag ttgcctgcgc aatgcctacc ttgacccgca ctgtgccggt ctgtgcgtca acaaccggcg acaattcacg gatattgccc tctgcttcaa tggaaggatc ggcaagcagg gcaaccgtta cgcccattcc aatgcgggca tgattcacca atgtttcctg cacatcgaaa atagcgtcgc gcgagccgtc ttccgcaatg gagaaagccg tttgcgctgc ctgcacgacc tgacccgttt cgatattgcg cgccgtaacg acgcctgaaa caggggcatg aagctcggta taggaaagct cgtcgcgtgc attgccaagc tggctttgcg cgctggaaag cgcactttgc gcggtgcgca atgcctgatc ggcctggtca tactggctgc gcgtggtaaa accctgcttc aacagcgcct gctggcgctt gaaattggcg tcggcctgca cgagctgcgc ttctgcggcg tccatggaag cctgcgcaac gcgcatattg gcgcgctgtt caacatcgtc gatgcgggcc agaagctgcc ccttttcgac atgctgcccg atatcgacca gccgttcgac cacctgacca ttgacgcgga acgatacatt gaccagagag cgcgcggcga cagccccgga aagcgtcaat ttcggctggt aatcgacaag cttcgcttcc cggaccgcaa ccggcacgac attgagtg
Expressed Protein Sequence
MAHHHHHHAD SLNVVPVAVR EAKLVDYQPK LTLSGAVAAR SLVNVSFRVN GQVVERLVDI GQHVEKGQLL ARIDDVEQRA NMRVAQASMD AAEAQLVQAD ANFKRQQALL KQGFTTRSQY DQADQALRTA QSALSSAQSQ LGNARDELSY TELHAPVSGV VTARNIETGQ VVQAAQTAFS IAEDGSRDAI FDVQETLVNH ARIGMGVTVA LLADPSIEAE GNIRELSPVV DAQTGTVRVK VGIAQATAQM ALGAIVTGTV HMDARQVIVL PWSAMASEAG RTAVWRVDRD THVATLVPIK VLAYEKERVI VESGLNNGEL IVTKGAQMLR SGEPVEIMQG PEGAGSQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCTGATT CACTCAATGT CGTGCCGGTT GCGGTCCGGG AAGCGAAGCT TGTCGATTAC CAGCCGAAAT TGACGCTTTC CGGGGCTGTC GCCGCGCGCT CTCTGGTCAA TGTATCGTTC CGCGTCAATG GTCAGGTGGT CGAACGGCTG GTCGATATCG GGCAGCATGT CGAAAAGGGG CAGCTTCTGG CCCGCATCGA CGATGTTGAA CAGCGCGCCA ATATGCGCGT TGCGCAGGCT TCCATGGACG CCGCAGAAGC GCAGCTCGTG CAGGCCGACG CCAATTTCAA GCGCCAGCAG GCGCTGTTGA AGCAGGGTTT TACCACGCGC AGCCAGTATG ACCAGGCCGA TCAGGCATTG CGCACCGCGC AAAGTGCGCT TTCCAGCGCG CAAAGCCAGC TTGGCAATGC ACGCGACGAG CTTTCCTATA CCGAGCTTCA TGCCCCTGTT TCAGGCGTCG TTACGGCGCG CAATATCGAA ACGGGTCAGG TCGTGCAGGC AGCGCAAACG GCTTTCTCCA TTGCGGAAGA CGGCTCGCGC GACGCTATTT TCGATGTGCA GGAAACATTG GTGAATCATG CCCGCATTGG AATGGGCGTA ACGGTTGCCC TGCTTGCCGA TCCTTCCATT GAAGCAGAGG GCAATATCCG TGAATTGTCG CCGGTTGTTG ACGCACAGAC CGGCACAGTG CGGGTCAAGG TAGGCATTGC GCAGGCAACT GCGCAGATGG CGCTTGGCGC AATCGTCACC GGAACTGTCC ATATGGATGC GCGGCAGGTG ATCGTGCTGC CATGGAGCGC GATGGCCTCG GAGGCGGGAC GAACGGCCGT CTGGCGCGTG GATAGGGACA CGCATGTCGC GACACTCGTT CCGATCAAGG TGCTGGCCTA TGAAAAAGAG CGCGTGATCG TCGAAAGCGG CCTTAATAAC GGTGAGCTTA TCGTGACCAA AGGTGCGCAG ATGCTGCGCT CCGGCGAGCC GGTAGAGATC ATGCAGGGGC CTGAAGGAGC TGGCTCGCAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT