BrmeA.00107.a

Macrolide-specific efflux protein maca

CENTER ID: BrmeA.00107.a
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00107.a.A2.GE33699 domain1 46 408
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.396
RefSeq: NP_539276.1
UniProt: Q8YIT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKSSDSSQL GQQPASARKR AKGKRRWWLW LSAAAIIIGA GWYFYGSYKA DGEKSSYLAE TVTRGDIENA ITAVGTLSAL RSVNVGAQVS GQLKSVKVEV GDHVKQGQLI AEIDPSPFEK KVEIAGAQLD NLKAQLLSKD AQLTLKKLNA ARQKSLLATR GVSQATVDQA NADLLMAEAE VKALNAQIRQ QEAQLASDKV DLGYTSIYSP MEGTVVDEAA KEGETLNAVQ SAPTIVTVAD LKVMTVEAQV SEADISKLKP GMPVYFTLLG QPDKRFTGTL RQIKPTPATD NNVVLYYALF DVPNPTGELM IDMSAQVYFV LGEAKDVLVV PTAALTDKQK GGKPQVTVKV ADESGTLVER PVKVGVRNRV LAEIQSGLEE GDRVVVTAAT GNGAAGGERG RRPSMRFF
NT Sequence
gtgcttaaat ctagtgattc ttctcaatta ggccagcaac cggcatcggc ccgcaaacgc gccaaaggaa agcgccgctg gtggctctgg ctttcagccg ccgccatcat catcggcgcg ggctggtact tctacggctc ttataaggct gacggcgaga agagcagcta tcttgcggag actgtcaccc gtggtgacat cgaaaacgcc atcaccgccg tcggcacgct gagcgcattg cgcagcgtca acgttggcgc gcaggtttcc ggccagttga aaagcgtgaa ggtggaagtg ggcgatcacg tcaaacaagg ccagctcatt gccgaaatcg atccttcacc gtttgaaaag aaggtggaga tcgcaggcgc ccagctcgac aatctgaaag cccaattgct cagcaaggac gcacaactga cgctgaagaa gctcaatgcc gcgcggcaga aatccctcct cgccacacgc ggcgtctcgc aggcgaccgt cgatcaggcc aatgccgatc ttttaatggc cgaagcggag gtaaaggcgc tcaacgcaca aatccgccag caggaagcac agcttgcaag cgacaaggtc gatctcggct acacctccat ctatagcccc atggaaggca cggtcgttga cgaggctgcc aaggaaggcg aaacgctcaa tgccgtgcag agcgcaccca ccattgtcac ggtcgcagac ctgaaagtaa tgacggtgga agcacaggtt tcggaagccg acatttccaa gctgaagccg ggaatgcctg tctatttcac gctgcttggc cagcccgaca agcgttttac cggcacgctt cgccagatca agccgacacc tgcgacagat aacaatgtcg ttctctatta tgccctgttc gacgtgccga acccgactgg cgaattgatg atcgacatga gtgcgcaggt ctatttcgtt ctgggtgagg cgaaggacgt gctcgttgtc cccacggcag cccttactga caagcagaaa ggcggcaagc cgcaagtcac cgtcaaggtg gccgacgaga gcggtaccct cgtggagcgc ccggtcaagg tcggcgtgcg caaccgcgtg ctggccgaaa tccagagcgg gcttgaggaa ggcgatcgcg tggttgtcac cgccgccacc ggcaatggcg cagcgggagg cgaacgcgga cgcagaccca gtatgcgctt cttctga
Details for BrmeA.00107.a.A2.GE33699
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGSYKADGEK SSYLAETVTR GDIENAITAV GTLSALRSVN VGAQVSGQLK SVKVEVGDHV KQGQLIAEID PSPFEKKVEI AGAQLDNLKA QLLSKDAQLT LKKLNAARQK SLLATRGVSQ ATVDQANADL LMAEAEVKAL NAQIRQQEAQ LASDKVDLGY TSIYSPMEGT VVDEAAKEGE TLNAVQSAPT IVTVADLKVM TVEAQVSEAD ISKLKPGMPV YFTLLGQPDK RFTGTLRQIK PTPATDNNVV LYYALFDVPN PTGELMIDMS AQVYFVLGEA KDVLVVPTAA LTDKQKGGKP QVTVKVADES GTLVERPVKV GVRNRVLAEI QSGLEEGDRV VVTAATGNGA AGGERGRRPS MRFF
Validated NT Sequence
ttattagaag aagcgcatac tgggtctgcg tccgcgttcg cctcccgctg cgccattgcc ggtggcggcg gtgacaacca cgcgatcgcc ttcctcaagc ccgctctgga tttcggccag cacgcggttg cgcacgccga ccttgaccgg gcgctccacg agggtaccgc tctcgtcggc caccttgacg gtgacttgcg gcttgccgcc tttctgcttg tcagtaaggg ctgccgtggg gacaacgagc acgtccttcg cctcacccag aacgaaatag acctgcgcac tcatgtcgat catcaattcg ccagtcgggt tcggcacgtc gaacagggca taatagagaa cgacattgtt atctgtcgca ggtgtcggct tgatctggcg aagcgtgccg gtaaaacgct tgtcgggctg gccaagcagc gtgaaataga caggcattcc cggcttcagc ttggaaatgt cggcttccga aacctgtgct tccaccgtca ttactttcag gtctgcgacc gtgacaatgg tgggtgcgct ctgcacggca ttgagcgttt cgccttcctt ggcagcctcg tcaacgaccg tgccttccat ggggctatag atggaggtgt agccgagatc gaccttgtcg cttgcaagct gtgcttcctg ctggcggatt tgtgcgttga gcgcctttac ctccgcttcg gccattaaaa gatcggcatt ggcctgatcg acggtcgcct gcgagacgcc gcgtgtggcg aggagggatt tctgccgcgc ggcattgagc ttcttcagcg tcagttgtgc gtccttgctg agcaattggg ctttcagatt gtcgagctgg gcgcctgcga tctccacctt cttttcaaac ggtgaaggat cgatttcggc aatgagctgg ccttgtttga cgtgatcgcc cacttccacc ttcacgcttt tcaactggcc ggaaacctgc gcgccaacgt tgacgctgcg caatgcgctc agcgtgccga cggcggtgat ggcgttttcg atgtcaccac gggtgacagt ctccgcaaga tagctgctct tctcgccgtc agccttataa gagcccatcg aaccaggacc ctgggtctga gcttccaggg ta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSYKADGE KSSYLAETVT RGDIENAITA VGTLSALRSV NVGAQVSGQL KSVKVEVGDH VKQGQLIAEI DPSPFEKKVE IAGAQLDNLK AQLLSKDAQL TLKKLNAARQ KSLLATRGVS QATVDQANAD LLMAEAEVKA LNAQIRQQEA QLASDKVDLG YTSIYSPMEG TVVDEAAKEG ETLNAVQSAP TIVTVADLKV MTVEAQVSEA DISKLKPGMP VYFTLLGQPD KRFTGTLRQI KPTPATDNNV VLYYALFDVP NPTGELMIDM SAQVYFVLGE AKDVLVVPTA ALTDKQKGGK PQVTVKVADE SGTLVERPVK VGVRNRVLAE IQSGLEEGDR VVVTAATGNG AAGGERGRRP SMRFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggctcttat aaggctgacg gcgagaagag cagctatctt gcggagactg tcacccgtgg tgacatcgaa aacgccatca ccgccgtcgg cacgctgagc gcattgcgca gcgtcaacgt tggcgcgcag gtttccggcc agttgaaaag cgtgaaggtg gaagtgggcg atcacgtcaa acaaggccag ctcattgccg aaatcgatcc ttcaccgttt gaaaagaagg tggagatcgc aggcgcccag ctcgacaatc tgaaagccca attgctcagc aaggacgcac aactgacgct gaagaagctc aatgccgcgc ggcagaaatc cctcctcgcc acacgcggcg tctcgcaggc gaccgtcgat caggccaatg ccgatctttt aatggccgaa gcggaggtaa aggcgctcaa cgcacaaatc cgccagcagg aagcacagct tgcaagcgac aaggtcgatc tcggctacac ctccatctat agccccatgg aaggcacggt cgttgacgag gctgccaagg aaggcgaaac gctcaatgcc gtgcagagcg cacccaccat tgtcacggtc gcagacctga aagtaatgac ggtggaagca caggtttcgg aagccgacat ttccaagctg aagccgggaa tgcctgtcta tttcacgctg cttggccagc ccgacaagcg ttttaccggc acgcttcgcc agatcaagcc gacacctgcg acagataaca atgtcgttct ctattatgcc ctgttcgacg tgccgaaccc gactggcgaa ttgatgatcg acatgagtgc gcaggtctat ttcgttctgg gtgaggcgaa ggacgtgctc gttgtcccca cggcagccct tactgacaag cagaaaggcg gcaagccgca agtcaccgtc aaggtggccg acgagagcgg taccctcgtg gagcgcccgg tcaaggtcgg cgtgcgcaac cgcgtgctgg ccgaaatcca gagcgggctt gaggaaggcg atcgcgtggt tgtcaccgcc gccaccggca atggcgcagc gggaggcgaa cgcggacgca gacccagtat gcgcttcttc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa