BrmeA.00058.a
Thioredoxin reductase (EC 1.8.1.9)
| CENTER ID: | BrmeA.00058.a |
| ORGANISM: | Brucella melitensis 16M |
| ASSOCIATED DISEASE: | Brucellosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.00058.a.A1.GE33700 | full length | 1 | 324 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrmeA.00058.a.A1.PS01310 | full length | 1 | 324 |
Structures
4JNQ
DEPOSITED: 3/15/2013
DETERMINATION: XRay
CLONE: BrmeA.00058.a.A1.GE33700
PROTEIN: BrmeA.00058.a.A1.PS01310
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127532 |
| PATRIC ID: | fig|224914.11.peg.575 |
| RefSeq: | NP_539429.1 |
| UniProt: | Q8YID2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQRHAPVIV IGSGPAGYTA AIYAARAMLK PVVIAGLQQG GQLMITTDVE NYPGYAEPVQ GPWMMEQMAR QAENVGAQIV HDIITEVETT VRPFRLKGDS GTIYTCDALI IATGAQAKWL GLESEQTFMG GGVSACATCD GFFYRGKDVV VVGGGNTAVE EALYLSHIAK SVTIVHRRDG FRAEKIMQDR LLSRENVSVV WNSVIDEILG TEARPPMGAT VTGVRLKNIV TGETQERATH GVFIAIGHAP AVSLFEGKLK QKPNGYLWTA PDSTATDVPG IFAAGDVTDD IYRQAVTAAG MGCMAALEAE RWLAAQEPLH EAAE
NT Sequence
atgacacagc gtcacgcgcc agttattgtt atcggctcag gtcctgctgg ttatacggca gcgatttatg cggcgcgcgc catgctgaaa cccgttgtca ttgccggttt gcagcagggc ggccagctga tgattacaac ggatgtggaa aactatccgg gctatgccga gccggtgcag gggccatgga tgatggagca gatggccagg caggctgaaa atgtcggtgc gcaaatcgtg catgacatca tcaccgaagt tgaaacgact gtgcggccgt tccgtctgaa gggggattcc ggcacgatct atacctgcga tgcgcttatt atcgcgaccg gtgcacaggc caagtggctg gggcttgaaa gcgagcagac cttcatgggc ggcggtgttt cggcttgtgc gacctgcgac ggtttcttct atcgcggcaa ggatgtggtt gtcgtcggcg gcggcaacac ggctgtcgaa gaggcgcttt atctgtcgca tattgccaaa agcgtcacga tcgtccatcg ccgtgacggt ttccgtgcgg aaaagatcat gcaggatcgc ctgctttcgc gcgagaacgt ctcggtggtg tggaacagtg tgatcgatga aatcctcggc accgaggccc ggcctccgat gggcgcgacc gtgacgggtg tacgtctgaa aaatattgtc accggcgaaa cgcaggaacg cgccacgcat ggcgttttca tcgcaatcgg ccacgctccg gcagtttcct tgtttgaggg caagttgaaa cagaagccaa acggctattt gtggaccgcg cccgattcca cggctaccga tgttcccggc attttcgcgg cgggcgacgt gacggacgac atttaccggc aggctgtcac ggctgcgggc atgggctgca tggccgcgct tgaggcggaa cgctggctgg ccgcacagga gccgctgcac gaagcggcgg agtag
Details for BrmeA.00058.a.A1.GE33700
| HARVESTED ON: | 9/28/2011 |
| SEQUENCED ON: | 10/4/2011 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MTQRHAPVIV IGSGPAGYTA AIYAARAMLK PVVIAGLQQG GQLMITTDVE NYPGYAEPVQ GPWMMEQMAR QAENVGAQIV HDIITEVETT VRPFRLKGDS GTIYTCDALI IATGAQAKWL GLESEQTFMG GGVSACATCD GFFYRGKDVV VVGGGNTAVE EALYLSHIAK SVTIVHRRDG FRAEKIMQDR LLSRENVSVV WNSVIDEILG TEARPPMGAT VTGVRLKNIV TGETQERATH GVFIAIGHAP AVSLFEGKLK QKPNGYLWTA PDSTATDVPG IFAAGDVTDD IYRQAVTAAG MGCMAALEAE RWLAAQEPLH EAAE
Validated NT Sequence
ttattactcc gccgcttcgt gcagcggctc ctgtgcggcc agccagcgtt ccgcctcaag cgcggccatg cagcccatgc ccgcagccgt gacagcctgc cggtaaatgt cgtccgtcac gtcgcccgcc gcgaaaatgc cgggaacatc ggtagccgtg gaatcgggcg cggtccacaa atagccgttt ggcttctgtt tcaacttgcc ctcaaacaag gaaactgccg gagcgtggcc gattgcgatg aaaacgccat gcgtggcgcg ttcctgcgtt tcgccggtga caatattttt cagacgtaca cccgtcacgg tcgcgcccat cggaggccgg gcctcggtgc cgaggatttc atcgatcaca ctgttccaca ccaccgagac gttctcgcgc gaaagcaggc gatcctgcat gatcttttcc gcacggaaac cgtcacggcg atggacgatc gtgacgcttt tggcaatatg cgacagataa agcgcctctt cgacagccgt gttgccgccg ccgacgacaa ccacatcctt gccgcgatag aagaaaccgt cgcaggtcgc acaagccgaa acaccgccgc ccatgaaggt ctgctcgctt tcaagcccca gccacttggc ctgtgcaccg gtcgcgataa taagcgcatc gcaggtatag atcgtgccgg aatccccctt cagacggaac ggccgcacag tcgtttcaac ttcggtgatg atgtcatgca cgatttgcgc accgacattt tcagcctgcc tggccatctg ctccatcatc catggcccct gcaccggctc ggcatagccc ggatagtttt ccacatccgt tgtaatcatc agctggccgc cctgctgcaa accggcaatg acaacgggtt tcagcatggc gcgcgccgca taaatcgctg ccgtataacc agcaggacct gagccgataa caataactgg cgcgtgacgc tgtgtcatcg aaccaggacc ctgggtctga gcttccaggg tacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQRHAPVI VIGSGPAGYT AAIYAARAML KPVVIAGLQQ GGQLMITTDV ENYPGYAEPV QGPWMMEQMA RQAENVGAQI VHDIITEVET TVRPFRLKGD SGTIYTCDAL IIATGAQAKW LGLESEQTFM GGGVSACATC DGFFYRGKDV VVVGGGNTAV EEALYLSHIA KSVTIVHRRD GFRAEKIMQD RLLSRENVSV VWNSVIDEIL GTEARPPMGA TVTGVRLKNI VTGETQERAT HGVFIAIGHA PAVSLFEGKL KQKPNGYLWT APDSTATDVP GIFAAGDVTD DIYRQAVTAA GMGCMAALEA ERWLAAQEPL HEAAE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacagcgt cacgcgccag ttattgttat cggctcaggt cctgctggtt atacggcagc gatttatgcg gcgcgcgcca tgctgaaacc cgttgtcatt gccggtttgc agcagggcgg ccagctgatg attacaacgg atgtggaaaa ctatccgggc tatgccgagc cggtgcaggg gccatggatg atggagcaga tggccaggca ggctgaaaat gtcggtgcgc aaatcgtgca tgacatcatc accgaagttg aaacgactgt gcggccgttc cgtctgaagg gggattccgg cacgatctat acctgcgatg cgcttattat cgcgaccggt gcacaggcca agtggctggg gcttgaaagc gagcagacct tcatgggcgg cggtgtttcg gcttgtgcga cctgcgacgg tttcttctat cgcggcaagg atgtggttgt cgtcggcggc ggcaacacgg ctgtcgaaga ggcgctttat ctgtcgcata ttgccaaaag cgtcacgatc gtccatcgcc gtgacggttt ccgtgcggaa aagatcatgc aggatcgcct gctttcgcgc gagaacgtct cggtggtgtg gaacagtgtg atcgatgaaa tcctcggcac cgaggcccgg cctccgatgg gcgcgaccgt gacgggtgta cgtctgaaaa atattgtcac cggcgaaacg caggaacgcg ccacgcatgg cgttttcatc gcaatcggcc acgctccggc agtttccttg tttgagggca agttgaaaca gaagccaaac ggctatttgt ggaccgcgcc cgattccacg gctaccgatg ttcccggcat tttcgcggcg ggcgacgtga cggacgacat ttaccggcag gctgtcacgg ctgcgggcat gggctgcatg gccgcgcttg aggcggaacg ctggctggcc gcacaggagc cgctgcacga agcggcggag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BrmeA.00058.a.A1.PS01310
| PURIFICATION DATe: | 12/16/2011 |
| CONCENTRATION: | 43.27mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTQRHAPVIV IGSGPAGYTA AIYAARAMLK PVVIAGLQQG GQLMITTDVE NYPGYAEPVQ GPWMMEQMAR QAENVGAQIV HDIITEVETT VRPFRLKGDS GTIYTCDALI IATGAQAKWL GLESEQTFMG GGVSACATCD GFFYRGKDVV VVGGGNTAVE EALYLSHIAK SVTIVHRRDG FRAEKIMQDR LLSRENVSVV WNSVIDEILG TEARPPMGAT VTGVRLKNIV TGETQERATH GVFIAIGHAP AVSLFEGKLK QKPNGYLWTA PDSTATDVPG IFAAGDVTDD IYRQAVTAAG MGCMAALEAE RWLAAQEPLH EAAE
Validated NT Sequence
ttattactcc gccgcttcgt gcagcggctc ctgtgcggcc agccagcgtt ccgcctcaag cgcggccatg cagcccatgc ccgcagccgt gacagcctgc cggtaaatgt cgtccgtcac gtcgcccgcc gcgaaaatgc cgggaacatc ggtagccgtg gaatcgggcg cggtccacaa atagccgttt ggcttctgtt tcaacttgcc ctcaaacaag gaaactgccg gagcgtggcc gattgcgatg aaaacgccat gcgtggcgcg ttcctgcgtt tcgccggtga caatattttt cagacgtaca cccgtcacgg tcgcgcccat cggaggccgg gcctcggtgc cgaggatttc atcgatcaca ctgttccaca ccaccgagac gttctcgcgc gaaagcaggc gatcctgcat gatcttttcc gcacggaaac cgtcacggcg atggacgatc gtgacgcttt tggcaatatg cgacagataa agcgcctctt cgacagccgt gttgccgccg ccgacgacaa ccacatcctt gccgcgatag aagaaaccgt cgcaggtcgc acaagccgaa acaccgccgc ccatgaaggt ctgctcgctt tcaagcccca gccacttggc ctgtgcaccg gtcgcgataa taagcgcatc gcaggtatag atcgtgccgg aatccccctt cagacggaac ggccgcacag tcgtttcaac ttcggtgatg atgtcatgca cgatttgcgc accgacattt tcagcctgcc tggccatctg ctccatcatc catggcccct gcaccggctc ggcatagccc ggatagtttt ccacatccgt tgtaatcatc agctggccgc cctgctgcaa accggcaatg acaacgggtt tcagcatggc gcgcgccgca taaatcgctg ccgtataacc agcaggacct gagccgataa caataactgg cgcgtgacgc tgtgtcatcg aaccaggacc ctgggtctga gcttccaggg tacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQRHAPVI VIGSGPAGYT AAIYAARAML KPVVIAGLQQ GGQLMITTDV ENYPGYAEPV QGPWMMEQMA RQAENVGAQI VHDIITEVET TVRPFRLKGD SGTIYTCDAL IIATGAQAKW LGLESEQTFM GGGVSACATC DGFFYRGKDV VVVGGGNTAV EEALYLSHIA KSVTIVHRRD GFRAEKIMQD RLLSRENVSV VWNSVIDEIL GTEARPPMGA TVTGVRLKNI VTGETQERAT HGVFIAIGHA PAVSLFEGKL KQKPNGYLWT APDSTATDVP GIFAAGDVTD DIYRQAVTAA GMGCMAALEA ERWLAAQEPL HEAAE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACAGCGT CACGCGCCAG TTATTGTTAT CGGCTCAGGT CCTGCTGGTT ATACGGCAGC GATTTATGCG GCGCGCGCCA TGCTGAAACC CGTTGTCATT GCCGGTTTGC AGCAGGGCGG CCAGCTGATG ATTACAACGG ATGTGGAAAA CTATCCGGGC TATGCCGAGC CGGTGCAGGG GCCATGGATG ATGGAGCAGA TGGCCAGGCA GGCTGAAAAT GTCGGTGCGC AAATCGTGCA TGACATCATC ACCGAAGTTG AAACGACTGT GCGGCCGTTC CGTCTGAAGG GGGATTCCGG CACGATCTAT ACCTGCGATG CGCTTATTAT CGCGACCGGT GCACAGGCCA AGTGGCTGGG GCTTGAAAGC GAGCAGACCT TCATGGGCGG CGGTGTTTCG GCTTGTGCGA CCTGCGACGG TTTCTTCTAT CGCGGCAAGG ATGTGGTTGT CGTCGGCGGC GGCAACACGG CTGTCGAAGA GGCGCTTTAT CTGTCGCATA TTGCCAAAAG CGTCACGATC GTCCATCGCC GTGACGGTTT CCGTGCGGAA AAGATCATGC AGGATCGCCT GCTTTCGCGC GAGAACGTCT CGGTGGTGTG GAACAGTGTG ATCGATGAAA TCCTCGGCAC CGAGGCCCGG CCTCCGATGG GCGCGACCGT GACGGGTGTA CGTCTGAAAA ATATTGTCAC CGGCGAAACG CAGGAACGCG CCACGCATGG CGTTTTCATC GCAATCGGCC ACGCTCCGGC AGTTTCCTTG TTTGAGGGCA AGTTGAAACA GAAGCCAAAC GGCTATTTGT GGACCGCGCC CGATTCCACG GCTACCGATG TTCCCGGCAT TTTCGCGGCG GGCGACGTGA CGGACGACAT TTACCGGCAG GCTGTCACGG CTGCGGGCAT GGGCTGCATG GCCGCGCTTG AGGCGGAACG CTGGCTGGCC GCACAGGAGC CGCTGCACGA AGCGGCGGAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA