BrmeA.00057.d

O-antigen export system, ATP-binding protein Wzt

CENTER ID: BrmeA.00057.d
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00057.d.B1.GE36673 Full length( BrmeA.00057.d ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_1580
BV-BRC: fig|224914.11.peg.1580
RefSeq: NP_540333.1
UniProt: F8WJX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAYGYQLHLV LRCEPPPNLR GSSCGDACGE ESHFLPMIQP SITLSNVHLH YAASAFKERS VKTLVNALLS MRRSAGANIE DIHALKGISV DIARGERVAL IGHNGAGKST FLKTIAGLYP ISSGTLKVTG TVRSLFDIGL GFEPDATGRE NILYRGLLLG LTPRFMREIE DEIIEFADLG DFIDYPIKTY SAGMQVRLAF AISTAVDGDI LLLDEVIGAG DAAFMTKAKA RIMNMVEKAE IMVLASHDLA NVRQLCTRAL VFKAGTIAFD GRVEDAISFY NSGMGAIA
NT Sequence
ATGGCCTACG GCTACCAATT ACATTTGGTG CTTAGGTGTG AGCCTCCTCC TAACCTGCGT GGCAGTAGCT GTGGGGATGC GTGCGGAGAA GAGAGCCATT TTTTACCTAT GATCCAGCCA TCGATTACCC TGTCAAATGT TCATCTGCAC TACGCTGCAT CAGCGTTCAA AGAACGCTCA GTCAAAACTC TAGTAAACGC CTTATTGAGT ATGCGGCGCA GCGCAGGAGC AAACATTGAA GACATCCATG CCCTAAAGGG TATTTCTGTA GATATAGCGC GGGGCGAACG CGTTGCCCTG ATAGGTCACA ACGGGGCTGG CAAAAGTACG TTCTTGAAAA CTATAGCCGG TCTCTACCCT ATATCATCTG GGACATTAAA AGTGACGGGT ACCGTAAGAT CCCTGTTCGA TATTGGTCTT GGGTTTGAGC CTGATGCAAC TGGCCGTGAG AATATTCTTT ACCGTGGGTT GCTTCTCGGA CTAACGCCAC GTTTCATGCG AGAGATCGAG GATGAGATCA TCGAGTTCGC GGATCTCGGC GATTTTATCG ATTATCCAAT CAAAACTTAT TCTGCCGGCA TGCAAGTTCG GCTCGCCTTC GCGATTTCGA CAGCAGTCGA CGGCGACATA CTCCTTCTAG ACGAAGTTAT AGGTGCAGGT GATGCGGCAT TCATGACTAA GGCGAAGGCC CGCATAATGA ATATGGTCGA GAAGGCTGAG ATAATGGTTC TAGCAAGCCA TGACCTTGCG AACGTCCGTC AGCTTTGCAC ACGAGCATTG GTTTTCAAAG CCGGCACAAT TGCATTTGAT GGCAGGGTAG AAGACGCGAT TTCCTTCTAT AACTCGGGAA TGGGAGCTAT AGCA
Details for BrmeA.00057.d.B1.GE36673
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM AYGYQLHLVL RCEPPPNLRG SSCGDACGEE SHFLPMIQPS ITLSNVHLHY AASAFKERSV KTLVNALLSM RRSAGANIED IHALKGISVD IARGERVALI GHNGAGKSTF LKTIAGLYPI SSGTLKVTGT VRSLFDIGLG FEPDATGREN ILYRGLLLGL TPRFMREIED EIIEFADLGD FIDYPIKTYS AGMQVRLAFA ISTAVDGDIL LLDXVIGAGD AAFMTXAXAR IMNMVEXAEI MVLASHDLAN VRQLCTRALV FKAGTIAFDG RVEXXISFYN SGMGAIA
Validated NT Sequence
nnnnnnnnnn nnctagaana ttttnnttan ctttaagaag gaganatacc atggctcacc accaccacca ccaccatatg gcctacggct accaattaca tttggtgctt aggtgtgagc ctcctcctaa cctgcgtggc agtagctgtg gggatgcgtg cggagaagag agccattttt tacctatgat ccagccatcg attaccctgt caaatgttca tctgcactac gctgcatcag cgttcaaaga acgctcagtc aaaactctag taaacgcctt attgagtatg cggcgcagcg caggagcaaa cattgaagac atccatgccc taaagggtat ttctgtagat atagcgcggg gcgaacgcgt tgccctgata ggtcacaacg gggctggcaa aagtacgttc ttgaaaacta tagccggtct ctaccctata tcatctggga cattaaaagt gacgggtacc gtaagatccc tgttcgatat tggtcttggg tttgagcctg atgcaactgg ccgtgagaat attctttacc gtgggttgct tctcggacta acgccacgtt tcatgcgaga gatcgaggat gagatcatcg agttcgcgga tctcggcgat tttatcgatt atccaatcaa aacttattct gccggcatgc aagttcggct cgccttcgcg atttcgacag cagtcgacgg cgacatactc cttctagacn aagttatagg tgcaggtgat gcggcattca tgactaangc gaangcccgc ataatgaata tggtcgagaa ngctgagata atggttctag caagccatga ccttgcgaac gtccgtcagc tttgcacacg agcattggtt ttcaaagccg gcacaattgc atttgatggc agggtagaan acncgatttc cttctataac tcgggaatgg gagctatagc ataantgann ananagnanc cnnnnnnnnn cccccnaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncnnn nnnnnnncnn nannnnnnnn nnnnnnnnnn n
Expected Protein Sequence
MAHHHHHHMA YGYQLHLVLR CEPPPNLRGS SCGDACGEES HFLPMIQPSI TLSNVHLHYA ASAFKERSVK TLVNALLSMR RSAGANIEDI HALKGISVDI ARGERVALIG HNGAGKSTFL KTIAGLYPIS SGTLKVTGTV RSLFDIGLGF EPDATGRENI LYRGLLLGLT PRFMREIEDE IIEFADLGDF IDYPIKTYSA GMQVRLAFAI STAVDGDILL LDEVIGAGDA AFMTKAKARI MNMVEKAEIM VLASHDLANV RQLCTRALVF KAGTIAFDGR VEDAISFYNS GMGAIA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct acggctacca attacatttg gtgcttaggt gtgagcctcc tcctaacctg cgtggcagta gctgtgggga tgcgtgcgga gaagagagcc attttttacc tatgatccag ccatcgatta ccctgtcaaa tgttcatctg cactacgctg catcagcgtt caaagaacgc tcagtcaaaa ctctagtaaa cgccttattg agtatgcggc gcagcgcagg agcaaacatt gaagacatcc atgccctaaa gggtatttct gtagatatag cgcggggcga acgcgttgcc ctgataggtc acaacggggc tggcaaaagt acgttcttga aaactatagc cggtctctac cctatatcat ctgggacatt aaaagtgacg ggtaccgtaa gatccctgtt cgatattggt cttgggtttg agcctgatgc aactggccgt gagaatattc tttaccgtgg gttgcttctc ggactaacgc cacgtttcat gcgagagatc gaggatgaga tcatcgagtt cgcggatctc ggcgatttta tcgattatcc aatcaaaact tattctgccg gcatgcaagt tcggctcgcc ttcgcgattt cgacagcagt cgacggcgac atactccttc tagacgaagt tataggtgca ggtgatgcgg cattcatgac taaggcgaag gcccgcataa tgaatatggt cgagaaggct gagataatgg ttctagcaag ccatgacctt gcgaacgtcc gtcagctttg cacacgagca ttggttttca aagccggcac aattgcattt gatggcaggg tagaagacgc gatttccttc tataactcgg gaatgggagc tatagcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at