BrmeA.00057.b

Sugar transport ATP-binding protein

CENTER ID: BrmeA.00057.b
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00057.b.A1.GE33701 full length 1 529
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|224914.11.peg.2665
RefSeq: NP_541339.1
UniProt: Q8YD16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLANPVSLD ERGALPDTFG KPLLEMRGIN KSFGVVKALS DVNFTVMPGE IHAFVGENGA GKSTLMKVLS GVYPHGSYEG AIYYDGEERQ FRDINDSEAL GIVIIHQELA LIPLMSIAEN IFLVNPPASR GVIDRSAVHK RTRELLKKVG LAETPDTLIT DIGIGKQQLV EIAKALSKRV RLLILDEPTA SLNENDSAAL LALLREFRAQ GITSILISHK LNEIREVADK ITVLRDGKAV ATLDCHAGEV EEDDIIRKMV DRDLESRYPK RDPKIGNVIF EVDNWSVYHP LHPDRHSVKN VSFKVRAGEI VGIAGLMGAG RTEFAMSLFG RSWGTNITGE ARINGKTVDI STVARAIKAG LAYVTEDRKK LGLVLGQNIS RNISLAHLRG VSPKGIIDDI REMKIANSYR DQMRIRCHNV YQETGTLSGG NQQKVVLSKW LFTGPDVLIL DEPTRGIDVG AKYDIYTIIN SLAESGKGVV VISSEMPELI GICDRIVVMH EGAFVGEVAG EEATQENIMR AIMRNTGKG
NT Sequence
atgaacctag ccaatcctgt ctcgctggac gagcgtgggg cactgccgga tactttcgga aagcctcttc ttgaaatgcg tgggatcaac aagtccttcg gtgtcgtgaa ggcgctgagc gatgtgaact tcaccgtcat gccgggcgaa atacatgcct ttgtgggtga aaacggcgcg ggaaaatcca cgctgatgaa ggtgctttcg ggtgtttatc cccatggaag ctatgaaggc gcgatctatt acgacgggga agagcggcag tttcgggata ttaacgattc cgaggcgctc ggcatcgtca tcattcatca ggaattggcc ctcattccgc tgatgtcgat tgccgagaat atttttctcg tcaatcctcc ggcaagccgg ggtgtcatcg accgctccgc cgtgcacaag cggacgcggg agctattgaa aaaggtcggt ctggcggaaa cgcccgatac gctcatcacc gatatcggca ttggcaagca gcagcttgtt gaaatcgcca aggcgctttc caagcgtgtg cggctgttga tcctcgatga accaacagca agcctcaacg aaaatgacag tgcggcgctt ctggcgctgc tgcgcgagtt tcgcgcgcag ggcatcacct cgatcctcat ttcgcacaag ctcaatgaaa tccgcgaagt ggccgacaag attaccgttc tgcgcgacgg caaggcggtt gcgacacttg attgccatgc gggcgaagtg gaggaagacg atatcatccg caagatggtg gaccgcgatc tggagagccg ttatcccaag cgcgatccca agatcggcaa tgtcatcttc gaggtcgata actggtcggt ctatcatccg ctgcatcccg atcggcattc ggtcaagaac gtgtcattca aggtgcgggc gggcgagatc gtcggcattg ccggattgat gggagcgggg cgcacggaat tcgccatgag cctgtttggc cgttcatggg gcacgaatat cactggcgag gcgcgcatca atggcaagac ggtggatatt tccaccgtgg cgcgggcaat caaggcgggc cttgcctatg ttaccgagga tcgcaagaag cttggcctgg ttcttggcca gaatatttcg cgcaatatct cgctggcgca cttacgcggc gtgagcccga aaggcattat cgacgatatc cgggaaatga agatcgcgaa ttcctatcgc gaccagatgc gtattcgctg tcacaatgtc tatcaggaaa ccggcacgct ttcgggcggc aaccagcaga aggtggtgct gtcgaaatgg cttttcactg gccccgacgt gctgatcctg gacgagccga cacgcggtat cgatgttggc gcgaaatatg acatttacac aatcatcaat tcactggcgg aaagcggcaa gggcgttgtc gtcatttctt cggaaatgcc ggagctgatc ggcatttgcg accgcatcgt cgtcatgcat gagggcgcct tcgtcggcga agtggcaggc gaggaagcaa cgcaggaaaa catcatgcgc gccatcatgc gaaacacggg aaaagggtaa
Details for BrmeA.00057.b.A1.GE33701
HARVESTED ON: 9/28/2011
SEQUENCED ON: 10/4/2011
EXPECTED MW: 60kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 70
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLANPVSL DERGALPDTF GKPLLEMRGI NKSFGVVKAL SDVNFTVMPG EIHAFVGENG AGKSTLMKVL SGVYPHGSYE GAIYYDGEER QFRDINDSEA LGIVIIHQEL ALIPLMSIAE NIFLVNPPAS RGVIDRSAVH KRTRELLKKV GLAETPDTLI TDIGIGKQQL VEIAKALSKR VRLLILDEPT ASLNENDSAA LLALLREFRA QGITSILISH KLNEIREVAD KITVLRDGXR LRHLIAMRAK WRKTISSARW WXAIWRAVIP SAIPRSAMSS SRSITGXSII RCIPIGIXSR XCHSXCGXXX SXXLXXXWXR XXXXXXXXWG XXXLXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
tttntttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaaccta gccaatcctg tctcgctgga cgagcgtggg gcactgccgg atactttcgg aaagcctctt cttgaaatgc gtgggatcaa caagtccttc ggtgtcgtga aggcgctgag cgatgtgaac ttcaccgtca tgccgggcga aatacatgcc tttgtgggtg aaaacggcgc gggaaaatcc acgctgatga aggtgctttc gggtgtttat ccccatggaa gctatgaagg cgcgatctat tacgacgggg aagagcggca gtttcgggat attaacgatt ccgaggcgct cggcatcgtc atcattcatc aggaattggc cctcattccg ctgatgtcga ttgccgagaa tatttttctc gtcaatcctc cggcaagccg gggtgtcatc gaccgctccg ccgtgcacaa gcggacgcgg gagctattga aaaaggtcgg tctggcggaa acgcccgata cgctcatcac cgatatcggc attggcaagc agcagcttgt tgaaatcgcc aaggcgcttt ccaagcgtgt gcggctgttg atcctcgatg aaccaacagc aagcctcaac gaaaatgaca gtgcggcgct tctggcgctg ctgcgcgagt ttcgcgcgca gggcatcacc tcgatcctca tttcgcacaa gctcaatgaa atccgcgaag tggccgacaa gattaccgtt ctgcgcgacg gnnngcggtt gcgacacttg attgccatgc gggcgaagtg gaggaagacg atatcatccg caagatggtg gancgcgatc tggagagccg ttatcccnag cgcgatcccn agatcggcaa tgtcatcttc gaggtcgata actggtcngt ctatcatccg ctgcatcccg atcggcattc ngtcaagaan gtgtcattca nggtgcgggc nggnnnnntc gtnnncattg nnnnanngat ggnnncgggn nnanngnnnn nntgnncnnt tntggggnnn nanntnnctg gnnnnnnnnn natnnngnnn nngnnngnnn nnnnnnnnnn gnnnnannnn nnnnnnnnnn cnnnnnnnnn nnannnnnnn nnnnnnnnaa nnnntt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLANPVSL DERGALPDTF GKPLLEMRGI NKSFGVVKAL SDVNFTVMPG EIHAFVGENG AGKSTLMKVL SGVYPHGSYE GAIYYDGEER QFRDINDSEA LGIVIIHQEL ALIPLMSIAE NIFLVNPPAS RGVIDRSAVH KRTRELLKKV GLAETPDTLI TDIGIGKQQL VEIAKALSKR VRLLILDEPT ASLNENDSAA LLALLREFRA QGITSILISH KLNEIREVAD KITVLRDGKA VATLDCHAGE VEEDDIIRKM VDRDLESRYP KRDPKIGNVI FEVDNWSVYH PLHPDRHSVK NVSFKVRAGE IVGIAGLMGA GRTEFAMSLF GRSWGTNITG EARINGKTVD ISTVARAIKA GLAYVTEDRK KLGLVLGQNI SRNISLAHLR GVSPKGIIDD IREMKIANSY RDQMRIRCHN VYQETGTLSG GNQQKVVLSK WLFTGPDVLI LDEPTRGIDV GAKYDIYTII NSLAESGKGV VVISSEMPEL IGICDRIVVM HEGAFVGEVA GEEATQENIM RAIMRNTGKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacctagcc aatcctgtct cgctggacga gcgtggggca ctgccggata ctttcggaaa gcctcttctt gaaatgcgtg ggatcaacaa gtccttcggt gtcgtgaagg cgctgagcga tgtgaacttc accgtcatgc cgggcgaaat acatgccttt gtgggtgaaa acggcgcggg aaaatccacg ctgatgaagg tgctttcggg tgtttatccc catggaagct atgaaggcgc gatctattac gacggggaag agcggcagtt tcgggatatt aacgattccg aggcgctcgg catcgtcatc attcatcagg aattggccct cattccgctg atgtcgattg ccgagaatat ttttctcgtc aatcctccgg caagccgggg tgtcatcgac cgctccgccg tgcacaagcg gacgcgggag ctattgaaaa aggtcggtct ggcggaaacg cccgatacgc tcatcaccga tatcggcatt ggcaagcagc agcttgttga aatcgccaag gcgctttcca agcgtgtgcg gctgttgatc ctcgatgaac caacagcaag cctcaacgaa aatgacagtg cggcgcttct ggcgctgctg cgcgagtttc gcgcgcaggg catcacctcg atcctcattt cgcacaagct caatgaaatc cgcgaagtgg ccgacaagat taccgttctg cgcgacggca aggcggttgc gacacttgat tgccatgcgg gcgaagtgga ggaagacgat atcatccgca agatggtgga ccgcgatctg gagagccgtt atcccaagcg cgatcccaag atcggcaatg tcatcttcga ggtcgataac tggtcggtct atcatccgct gcatcccgat cggcattcgg tcaagaacgt gtcattcaag gtgcgggcgg gcgagatcgt cggcattgcc ggattgatgg gagcggggcg cacggaattc gccatgagcc tgtttggccg ttcatggggc acgaatatca ctggcgaggc gcgcatcaat ggcaagacgg tggatatttc caccgtggcg cgggcaatca aggcgggcct tgcctatgtt accgaggatc gcaagaagct tggcctggtt cttggccaga atatttcgcg caatatctcg ctggcgcact tacgcggcgt gagcccgaaa ggcattatcg acgatatccg ggaaatgaag atcgcgaatt cctatcgcga ccagatgcgt attcgctgtc acaatgtcta tcaggaaacc ggcacgcttt cgggcggcaa ccagcagaag gtggtgctgt cgaaatggct tttcactggc cccgacgtgc tgatcctgga cgagccgaca cgcggtatcg atgttggcgc gaaatatgac atttacacaa tcatcaattc actggcggaa agcggcaagg gcgttgtcgt catttcttcg gaaatgccgg agctgatcgg catttgcgac cgcatcgtcg tcatgcatga gggcgccttc gtcggcgaag tggcaggcga ggaagcaacg caggaaaaca tcatgcgcgc catcatgcga aacacgggaa aagggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa