SSGCID: Target Page

BrmeA.00057.a

Maltose/maltodextrin transport ATP-binding protein malk

CENTER ID:	BrmeA.00057.a
ORGANISM:	Brucella melitensis 16M
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmeA.00057.a.A1.GE33702	full length		1	363

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|224914.11.peg.1896
RefSeq:	NP_540630.1
UniProt:	Q8YF14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAQLSIKNLV KRYGSIEVVH GINLEIADKE FVALVGPSGC GKSTTLRMIA GLESISGGTL EIGGKVVNDL PPRDRNISMV FQSYALYPHM SVRENMGFSL KIAKQPQAEI DRRVNEAAAV LGLEALMDRR PAQLSGGQRQ RVAMGRAIVR NPEVFLFDEP LSNLDAKLRT QMRTEIKKLH AKVQSTVVYV THDQVEAMTL ADRIVIMRDG HIEQAGTPDE VFKRPATQIV AGFIGSPPMN MAEATVKGDE LLFANGDRLP LPARFKARVG EGAKVTFGLR PDDIFPKGHG LSTGDGVHEK ELRVVITEPL GNETLVFAEF AGREWVARML NPRPVQPGES IAMQFDLAQA HLFDAATGKS LTV

NT Sequence

atggcacagc tttccatcaa aaatctcgtc aagcgttatg gcagcatcga agtggtgcat ggcatcaatc tcgaaatcgc cgacaaggag tttgtcgcgc tggtcggccc gtccggttgc ggaaaatcca cgacgctgcg gatgattgcc ggcctggaat ccatctccgg tggcacgctt gaaatcggcg gcaaagtcgt caacgacttg cccccgcgtg atcgcaacat ctccatggtg ttccagtcct atgcgctcta tccgcatatg agcgtacgcg agaacatggg tttttcgctg aagattgcaa agcagccgca ggcggaaatc gaccgccgcg tcaacgaggc ggcggctgtt ctggggcttg aagcattgat ggaccgccgc cccgcgcagc tttcgggcgg ccagcgccag cgtgtcgcca tggggcgcgc catcgtgcgc aacccggaag tctttctctt cgatgagccg ctctcgaacc tcgatgccaa actgcgtacg cagatgcgca ccgaaatcaa gaagctgcac gccaaggtgc agtctacggt cgtctatgtg acccacgatc aggtcgaggc gatgacgctg gccgaccgca ttgtcatcat gcgcgacggc catatcgaac aggcgggcac cccggatgaa gtgttcaagc gcccggcgac gcaaatcgtt gcgggcttca tcggttcgcc gccgatgaac atggccgaag cgaccgtgaa gggtgacgag cttcttttcg ccaatggcga ccgcctgccg ctgcctgccc ggttcaaggc aagggtgggc gagggcgcaa aggttacttt cggcctgcgg cctgatgata ttttcccgaa aggtcacggc ctttcgaccg gcgatggcgt gcatgagaaa gaattgcgcg tcgtcatcac cgagccgctt ggcaatgaaa cactggtctt tgcggaattt gccgggcgcg aatgggtggc gcgtatgctg aacccgcgcc cggtgcagcc gggtgagagc attgccatgc agttcgatct ggcgcaggcg catcttttcg atgccgctac cggcaagagt ctgacggtgt aa

Details for BrmeA.00057.a.A1.GE33702

HARVESTED ON:	9/28/2011
SEQUENCED ON:	10/4/2011
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

ttntnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggcacagc tttccatcaa aaatctcgtc aagcgttatg gcagcatcga agtggtgcat ggcatcaatc tcgaaatcgc cgacaaggag tttgtcgcgc tggtcggccc gtccggttgc ggaaaatcca cgacgctgcg gatgattgcc ggcctggaat ccatctccgg tggcacgctt gaaatcggcg gcaaagtcgt caacgacttg cccccgcgtg atcgcaacat ctccatggtg ttccagtcct atgcgctcta tccgcatatg agcgtacgcg agaacatggg tttttcgctg aagattgcaa agcagccgca ggcggaaatc gaccgccgcg tcaacgaggc ggcggctgtt ctggggcttg aagcattgat ggaccgccgc cccgcgcagc tttcgggcgg ccagcgccag cgtgtcgcca tggggcgcgc catcgtgcgc aacccggaag tctttctctt cgatgagccg ctctcgaacc tcgatgccaa actgcgtacg cagatgcgca ccgaaatcaa gaagctgcac gccaaggtgc agtctacggt cgtctatgtg acccacgatc aggtcgaggc gatgacgctg gccgaccgca ttgtcatcat gcgcgacggc catatcgaac aggcgggcac cccggatgaa gtgttcaagc gcccggcgac gcnntcgttg cgggcttcat cggttcgccg ccgatgaaca tggccgaagc gaccgtgaag ggtgacgagc ttcttttcgc caatggcgac cgcctgccgc tgcctgcccg gttcnaggca agggtgggcg agggcgcaaa ggttactttn ngcctgcggc ctgatgatat tttcccgaaa ngtcanggcc tttcgancgg cgatggcgtg catgagaaag aantgcgcgt cgtcatcacc gannncttgg nnngaaanac tngnnnttgn nnantnnnng nnncnanggg nggcgnntat gcnnnnnncn ccgnncannn nnnnnnnnca nnnnnnnnan tncgatctgn nnagnnnttn nnnncnnntn cngnnnnnnn ntnnannnnn nnnnnnnnnn nnannnnnna nnnnnctnnn nnnnnnnnnn nnnnnnnnnn nnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAQLSIKNL VKRYGSIEVV HGINLEIADK EFVALVGPSG CGKSTTLRMI AGLESISGGT LEIGGKVVND LPPRDRNISM VFQSYALYPH MSVRENMGFS LKIAKQPQAE IDRRVNEAAA VLGLEALMDR RPAQLSGGQR QRVAMGRAIV RNPEVFLFDE PLSNLDAKLR TQMRTEIKKL HAKVQSTVVY VTHDQVEAMT LADRIVIMRD GHIEQAGTPD EVFKRPATQI VAGFIGSPPM NMAEATVKGD ELLFANGDRL PLPARFKARV GEGAKVTFGL RPDDIFPKGH GLSTGDGVHE KELRVVITEP LGNETLVFAE FAGREWVARM LNPRPVQPGE SIAMQFDLAQ AHLFDAATGK SLTV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacagctt tccatcaaaa atctcgtcaa gcgttatggc agcatcgaag tggtgcatgg catcaatctc gaaatcgccg acaaggagtt tgtcgcgctg gtcggcccgt ccggttgcgg aaaatccacg acgctgcgga tgattgccgg cctggaatcc atctccggtg gcacgcttga aatcggcggc aaagtcgtca acgacttgcc cccgcgtgat cgcaacatct ccatggtgtt ccagtcctat gcgctctatc cgcatatgag cgtacgcgag aacatgggtt tttcgctgaa gattgcaaag cagccgcagg cggaaatcga ccgccgcgtc aacgaggcgg cggctgttct ggggcttgaa gcattgatgg accgccgccc cgcgcagctt tcgggcggcc agcgccagcg tgtcgccatg gggcgcgcca tcgtgcgcaa cccggaagtc tttctcttcg atgagccgct ctcgaacctc gatgccaaac tgcgtacgca gatgcgcacc gaaatcaaga agctgcacgc caaggtgcag tctacggtcg tctatgtgac ccacgatcag gtcgaggcga tgacgctggc cgaccgcatt gtcatcatgc gcgacggcca tatcgaacag gcgggcaccc cggatgaagt gttcaagcgc ccggcgacgc aaatcgttgc gggcttcatc ggttcgccgc cgatgaacat ggccgaagcg accgtgaagg gtgacgagct tcttttcgcc aatggcgacc gcctgccgct gcctgcccgg ttcaaggcaa gggtgggcga gggcgcaaag gttactttcg gcctgcggcc tgatgatatt ttcccgaaag gtcacggcct ttcgaccggc gatggcgtgc atgagaaaga attgcgcgtc gtcatcaccg agccgcttgg caatgaaaca ctggtctttg cggaatttgc cgggcgcgaa tgggtggcgc gtatgctgaa cccgcgcccg gtgcagccgg gtgagagcat tgccatgcag ttcgatctgg cgcaggcgca tcttttcgat gccgctaccg gcaagagtct gacggtgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa