BrmeA.00027.c

Butyryl-CoA dehydrogenase (EC 1.3.99.2)

CENTER ID: BrmeA.00027.c
ORGANISM: Brucella melitensis 16M
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmeA.00027.c.B1.GE36671 Full length( BrmeA.00027.c ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruMel92729_3157
BV-BRC: fig|224914.11.peg.3157
RefSeq: NP_541796.1
UniProt: Q8YBR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLLTDTQEQI REAARDFAQE RLAPGAAARD REHAFPRAEL TEMGALGFLG MLVPEEWGGS DLGMVAYALA LEEIAAGDGA CSTIVSVHSS VGCMPILRFG TEDQKRRFLP KMACGEWIGG FALTEPQAGS DASALKTRAR LDGDHYVIDG SKQFITSGKN GNVVIVFAVT DPAAGKKGIS AFIVPTDTPG YEVMSVEHKL GQHSSDTCAL GFTNMRVPVE NRLGAEGEGY KIALANLEGG RIGIAAQAVG MARAAFEAAR DYARERITFG KPIIEHQAVA FRLADMATRI ETARQMVLHA AALREAGKPC LTEASMAKLV ASEMAEQVCS AAIQIHGGYG YLADYRSSAS IAMSASARFM KAQATCSGSS SRAGYNQPST
NT Sequence
ATGCTTCTGA CCGATACGCA GGAACAGATC CGCGAAGCCG CCCGCGACTT CGCACAGGAG CGCCTTGCCC CCGGCGCCGC GGCGCGTGAC CGTGAACATG CATTTCCCCG CGCCGAACTG ACTGAAATGG GCGCGCTTGG CTTTCTCGGA ATGCTGGTGC CGGAAGAATG GGGCGGCTCC GACCTCGGCA TGGTTGCCTA TGCTCTGGCG CTGGAAGAGA TAGCGGCAGG CGACGGTGCC TGCTCGACCA TCGTATCGGT GCATTCCTCC GTCGGCTGTA TGCCGATCTT ACGTTTCGGC ACGGAAGACC AGAAGCGCCG CTTCCTGCCC AAAATGGCCT GTGGCGAATG GATCGGCGGC TTTGCCCTCA CCGAGCCGCA GGCCGGTTCC GATGCCTCCG CGCTGAAAAC GCGCGCCCGG CTCGACGGCG ATCATTATGT GATCGACGGT TCCAAGCAGT TCATCACATC AGGCAAGAAC GGCAATGTCG TGATCGTTTT TGCCGTTACC GATCCTGCCG CAGGCAAGAA GGGTATTTCC GCCTTCATCG TGCCCACCGA TACGCCAGGT TATGAGGTGA TGTCGGTCGA ACACAAGCTC GGCCAGCATT CATCCGACAC CTGTGCGCTC GGCTTCACCA ATATGCGCGT TCCGGTCGAA AACCGGCTGG GCGCTGAAGG CGAAGGCTAC AAGATCGCCC TTGCCAATCT GGAAGGCGGA CGCATCGGCA TCGCAGCCCA GGCGGTTGGC ATGGCGCGCG CCGCTTTCGA GGCGGCACGC GATTATGCCC GCGAACGCAT TACCTTCGGC AAGCCGATCA TCGAACATCA GGCCGTGGCC TTCCGCCTTG CCGACATGGC AACCCGGATC GAAACCGCCC GGCAGATGGT GTTACACGCC GCTGCCCTGC GCGAAGCAGG CAAGCCATGC CTTACCGAAG CTTCAATGGC AAAGCTGGTC GCATCGGAAA TGGCCGAACA GGTCTGTTCC GCAGCCATCC AGATTCATGG CGGCTATGGC TATCTGGCCG ATTATCGGTC GAGCGCATCT ATCGCGATGT CCGCGTCTGC CAGATTTATG AAGGCACAAG CGACGTGCAG CGGCTCGTCA TCGCGCGCGG GCTATAATCA GCCTTCGACC
Details for BrmeA.00027.c.B1.GE36671
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 42kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 88
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHML LTDTQEQIRE AARDFAQERL APGAAARDRE HAFPRAELTE MGALGFLGML VPEEWGGSDL GMVAYALALE EIAAGDGACS TIVSVHSSVG CMPILRFGTE DQKRRFLPKM ACGEWIGGFA LTEPQAGSDA SALKTRARLD GDHYVIDGSK QFITSGKNGN VVIVFAVTDP AAGKKGISAF IVPTDTPGYE VMSVEHKLGQ HSSDTCALGF TNMRVPVENR LGAEGEGYKI ALANLEGGRI GIAAXAVGMA RAAFEAARDY ARERITFGKP IIEHXXVXFR XXXXXTXIET AXAXGVHAAA XXXXXAMXXX XXLXASXXPX XLXPXXXX
Validated NT Sequence
cnctannnnn nnttnnttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgcttc tgaccgatac gcaggaacag atccgcgaag ccgcccgcga cttcgcacag gagcgccttg cccccggcgc cgcggcgcgt gaccgtgaac atgcatttcc ccgcgccgaa ctgactgaaa tgggcgcgct tggctttctc ggaatgctgg tgccggaaga atggggcggc tccgacctcg gcatggttgc ctatgctctg gcgctggaag agatagcggc aggcgacggt gcctgctcga ccatcgtatc ggtgcattcc tccgtcggct gtatgccgat cttacgtttc ggcacggaag accagaagcg ccgcttcctg cccaaaatgg cctgtggcga atggatcggc ggctttgccc tcaccgagcc gcaggccggt tccgatgcct ccgcgctgaa aacgcgcgcc cggctcgacg gcgatcatta tgtgatcgac ggttccaagc agttcatcac atcaggcaag aacggcaatg tcgtgatcgt ttttgccgtt accgatcctg ccgcaggcaa gaagggtatt tccgccttca tcgtgcccac cgatacgcca ggttatgagg tgatgtcggt cgaacacaag ctcggccagc attcatccga cacctgtgcg ctcggcttca ccaatatgcg cgttccggtc gaaaaccggc tgggcgctga aggcgaaggc tacaagatcg cccttgccaa tctggaaggc ggacgcatcg gcatcgcagc ccangcggtt ggcatggcgc gcgccgcttt cgaggcggca cgcgattatg cccgcgaacg cattaccttc ggcaagccga tcatcgaaca tcnnnccgtg gnnttccgcc ntgncnacnt ggnnacccng atcgaaaccg cccnggcana nggngtncac gccgctgccn tgcncnancn nnaagccatg ncnanntnnn nnaanctngn cgcatcnnan tngccnaann gnctgntccc anccntncnn nnttc
Expected Protein Sequence
MAHHHHHHML LTDTQEQIRE AARDFAQERL APGAAARDRE HAFPRAELTE MGALGFLGML VPEEWGGSDL GMVAYALALE EIAAGDGACS TIVSVHSSVG CMPILRFGTE DQKRRFLPKM ACGEWIGGFA LTEPQAGSDA SALKTRARLD GDHYVIDGSK QFITSGKNGN VVIVFAVTDP AAGKKGISAF IVPTDTPGYE VMSVEHKLGQ HSSDTCALGF TNMRVPVENR LGAEGEGYKI ALANLEGGRI GIAAQAVGMA RAAFEAARDY ARERITFGKP IIEHQAVAFR LADMATRIET ARQMVLHAAA LREAGKPCLT EASMAKLVAS EMAEQVCSAA IQIHGGYGYL ADYRSSASIA MSASARFMKA QATCSGSSSR AGYNQPST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc tgaccgatac gcaggaacag atccgcgaag ccgcccgcga cttcgcacag gagcgccttg cccccggcgc cgcggcgcgt gaccgtgaac atgcatttcc ccgcgccgaa ctgactgaaa tgggcgcgct tggctttctc ggaatgctgg tgccggaaga atggggcggc tccgacctcg gcatggttgc ctatgctctg gcgctggaag agatagcggc aggcgacggt gcctgctcga ccatcgtatc ggtgcattcc tccgtcggct gtatgccgat cttacgtttc ggcacggaag accagaagcg ccgcttcctg cccaaaatgg cctgtggcga atggatcggc ggctttgccc tcaccgagcc gcaggccggt tccgatgcct ccgcgctgaa aacgcgcgcc cggctcgacg gcgatcatta tgtgatcgac ggttccaagc agttcatcac atcaggcaag aacggcaatg tcgtgatcgt ttttgccgtt accgatcctg ccgcaggcaa gaagggtatt tccgccttca tcgtgcccac cgatacgcca ggttatgagg tgatgtcggt cgaacacaag ctcggccagc attcatccga cacctgtgcg ctcggcttca ccaatatgcg cgttccggtc gaaaaccggc tgggcgctga aggcgaaggc tacaagatcg cccttgccaa tctggaaggc ggacgcatcg gcatcgcagc ccaggcggtt ggcatggcgc gcgccgcttt cgaggcggca cgcgattatg cccgcgaacg cattaccttc ggcaagccga tcatcgaaca tcaggccgtg gccttccgcc ttgccgacat ggcaacccgg atcgaaaccg cccggcagat ggtgttacac gccgctgccc tgcgcgaagc aggcaagcca tgccttaccg aagcttcaat ggcaaagctg gtcgcatcgg aaatggccga acaggtctgt tccgcagcca tccagattca tggcggctat ggctatctgg ccgattatcg gtcgagcgca tctatcgcga tgtccgcgtc tgccagattt atgaaggcac aagcgacgtg cagcggctcg tcatcgcgcg cgggctataa tcagccttcg acctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat