BrmaA.19165.a

pantothenate kinase (PanK)-like protein; BMA-PNK-1, isoform e (Bm2143e)

CENTER ID: BrmaA.19165.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.19165.a.A2.GE39852 truncation 1 50 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0J9Y261

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGGDPTYDT MVTGEVKKGE NENRDGDVKD IRCEVVGRRQ RLLSLSVPPM PWFGIDIGGT LVKLVYFEPE DHNDFIESEE EILRRRKIQR YLVTGKAYGG TGVRDDHLRL CNVGINGRVG TIHFIRFPTD RMLDFVNLVK RKGFAEMSST VCATGGGSLK YAKEAADLGL QLHKTDELES LIKGIEFIAA TNPDECYYYE NPLNDILCRK VIWRWSHGRC STSDSVPESD KKDGLQYPYI VCNIGSGVSV LAVRGHNQFK RIGGSRYMRN YVWM
NT Sequence
atgtcgggtg gtgatcctac atacgatact atggtgactg gagaggtgaa aaagggtgag aatgaaaaca gggatggcga tgtaaaagat attcgatgtg aagtggttgg ccgaaggcaa agattacttt cactttctgt gcctccaatg ccatggtttg gtattgatat aggaggcaca ttggtaaaac ttgtctactt cgaaccggaa gatcataatg attttattga aagtgaagaa gaaatcttac gacgtaggaa aattcaacgt tatcttgtaa caggcaaagc ctatggtggt actggtgtaa gggatgatca cctacgtctc tgtaatgtcg gaatcaatgg cagagttggt actatacatt ttattcgatt tccaacagac agaatgcttg attttgtaaa tttggttaag aggaaggggt tcgccgaaat gagcagtaca gtatgtgcta ctgggggcgg ctctttaaaa tatgccaaag aagcggcgga tttgggtttg cagttgcaca aaactgatga attggaatcg ttgattaagg gtattgaatt tattgctgca acaaatcctg acgaatgcta ttattatgaa aaccccttga atgatatttt gtgtagaaaa gtaatttggc ggtggtcaca cggtcgctgc tcaacatccg attctgttcc tgaaagtgac aagaaggatg gcttgcagta cccatacatt gtttgcaata ttggtagtgg tgtgtctgtg ttagctgtac gtggtcacaa tcaatttaag cggattggag gcagtaggta tatgcgcaat tatgtgtgga tgtga
Details for BrmaA.19165.a.A2.GE39852
HARVESTED ON: 1/26/2016
SEQUENCED ON: 1/28/2016
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 110
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPWFGIDIG GTLVKLVYFE PEDHNDFIES EEEILRRRKI QRYLVTGKAY GGTGVRDDHL RLCNVGINGR VGTIHFIRFP TDRMLDFVNL VKRKGFAEMS STVCATGGGS LKYAKEAADL GLQLHKTDEL ESLIKGIEFI AATNPDECYY YENPLNDILC RKVIWRWSHG RCSTSDSVPE SDKKDGLQYP YIVCNIGSGV SVLAVRGHNQ FKRIGGSSIG GGFFQGICAI MCGCNKQHEQ VLQPSFSRIR LLT
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPWFGIDIG GTLVKLVYFE PEDHNDFIES EEEILRRRKI QRYLVTGKAY GGTGVRDDHL RLCNVGINGR VGTIHFIRFP TDRMLDFVNL VKRKGFAEMS STVCATGGGS LKYAKEAADL GLQLHKTDEL ESLIKGIEFI AATNPDECYY YENPLNDILC RKVIWRWSHG RCSTSDSVPE SDKKDGLQYP YIVCNIGSGV SVLAVRGHNQ FKRIGGSRYM RNYVWM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccatggttt ggtattgata taggaggcac attggtaaaa cttgtctact tcgaaccgga agatcataat gattttattg aaagtgaaga agaaatctta cgacgtagga aaattcaacg ttatcttgta acaggcaaag cctatggtgg tactggtgta agggatgatc acctacgtct ctgtaatgtc ggaatcaatg gcagagttgg tactatacat tttattcgat ttccaacaga cagaatgctt gattttgtaa atttggttaa gaggaagggg ttcgccgaaa tgagcagtac agtatgtgct actgggggcg gctctttaaa atatgccaaa gaagcggcgg atttgggttt gcagttgcac aaaactgatg aattggaatc gttgattaag ggtattgaat ttattgctgc aacaaatcct gacgaatgct attattatga aaaccccttg aatgatattt tgtgtagaaa agtaatttgg cggtggtcac acggtcgctg ctcaacatcc gattctgttc ctgaaagtga caagaaggat ggcttgcagt acccatacat tgtttgcaat attggtagtg gtgtgtctgt gttagctgta cgtggtcaca atcaatttaa gcggattgga ggcagtaggt atatgcgcaa ttatgtgtgg atgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa