BrmaA.18982.a

Calcium-transporting ATPase (EC 3.6.3.8)

CENTER ID: BrmaA.18982.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18982.a.HK12.GE00494 full length 1 1050
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18982.a.HG12.PD38292 full length 1 1050
BrmaA.18982.a.HG12.PD38293 full length 1 1050

External Resources

RESOURCE REFERENCE ID
UniProt: A8PF97

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENAHTKSTD EVLQYFGVSG DVGLTEKQVL QNREKYGSNE LPAEEGKKLW ELILEQFDDL LVKILLLAAI ISFVLALFEE HDDQTSAVTA FVEPFVILLI LIANATVGVW QERNAESAIE ALKEYEPEMA KVMREGKHGI QMIRANELVP GDIVEVSVGD KIPADLRLIK IYSTTLRIDQ SILTGESVSV IKYTDSVPDP RAVNQDKKNC LFSGTNVAAG KARGVVFGIG LNTEIGKIRT EMAETETDRT PLQQKLDEFG EQLSKVISII CVAVWAINIG HFNDPAHGGS WIKGAIYYFK IAVALAVAAI PEGLPAVITT CLALGTRRMA KKNAIVRSLP SVETLGCTSV ICSDKTGTLT TNQMSVSKMF VASKVTGDDI DFLEFTISGS TYEPSGQVFH HGRQINCASG EFEALTELAT ICAMCNDSSV DYNETKHMYE KVGEATETAL VVLCEKMNVY GTNKVGLSPR DLGNVCNRVI QQKWKKEFTL EFSRDRKSMS AFCIPSSGGT NAKMFVKGAP EGVLNRCTHV RVNGQRIPLT QKITQKIVDQ CIHYGTGRDT LRCLALGTID SPPDPRTMNL EDSSQFILYE KDITFVGVVG MLDPPRAEVI PSIKECRMAG IRVIMITGDN KNTAEAIGRR IGLFAEDEDS TGKAFTGREF DDLPPEQQSD ACRRAKLFAR VEPTHKSKIV EFLQSHGEIT AMTGDGVNDA PALKKSEIGI AMGSGTAVAK TASEMVLADD NFATIVAAVE EGRAIYNNMK QFIRYLISSN IGEVVSIFLV AALGIPEALI PVQLLWVNLV TDGLPATALG FNPPDLDIMD RPPRSAGESL ISKWLFFRYM AVGSYVGLAT VGAAMWWFLL YEDGPQISYY QLTHWMRCEI EPENFADLDC AVFEDAHPNA MALSVLVTIE MFNALNSLSE NQSLLVMPPW KNIWLMSSIA LSLSLHFVIL YVEILATIFQ ITPLTLVEWF AVLKISFPVI LLDEVLKFIA RNYIDGETVC RIGDYEKPVK KRVSHTLLTV VMLTTLCVGY FYWLLKPYWS QLMHAFSMDL QSNSNSFHSH SHDDL
NT Sequence
atggagaacg cccacacgaa atcaaccgat gaggtcttac agtatttcgg tgtcagtggt gatgttggcc ttaccgagaa acaggttctt caaaacagag aaaaatatgg ctccaatgaa ctgccagctg aagaagggaa gaaattatgg gaattaatat tggaacagtt cgacgatctc cttgtcaaaa ttttgcttct tgctgctatc atttcattcg tgctagcctt atttgaagaa cacgacgatc aaacatcagc ggtcactgca ttcgttgaac cttttgtaat tttgcttatc ttaatagcaa atgcaacggt cggtgtttgg caagagagga atgccgaaag tgctatagaa gctcttaaag aatacgaacc agagatggca aaagtaatgc gtgaagggaa gcacggaatt cagatgattc gtgctaatga acttgtgccg ggtgatatcg ttgaggtttc agtgggtgat aaaattcccg cagatttacg tttgataaaa atttattcaa caacattgcg tattgatcaa tcaatcctta ccggtgaatc agtttctgta attaaatata cggatagcgt acctgatcca agggctgtta atcaggataa gaagaattgt cttttctccg gaacaaacgt tgctgctggg aaggctcgtg gtgttgtttt tggtattggg ttaaatacgg aaattggtaa aatccgtacc gaaatggctg aaactgaaac cgatcgtaca ccgctacagc agaaacttga tgaattcggt gaacagttgt ccaaagttat ttcaattatt tgtgtggctg tgtgggcgat caatatcggt cacttcaacg atcctgctca tggtggttcg tggatcaaag gtgctatata ttacttcaaa attgctgttg cgcttgctgt tgctgctatc ccagaaggtc ttcctgctgt tatcacaact tgcttagctt tgggcacacg tcgaatggct aaaaaaaatg ctattgtgcg ttcgttacca tcagtggaaa ctttgggttg tacttccgta atttgctcag ataaaaccgg aacacttact actaatcaaa tgagtgtttc gaaaatgttt gttgcaagca aagtaaccgg cgacgatatt gatttcctcg aatttactat tagcggatca acttatgaac catcaggaca ggtatttcat catggacggc aaataaactg tgcaagtggt gaatttgagg ctttaacaga attggcaact atttgtgcaa tgtgcaacga ctcttctgtt gactataacg aaacgaaaca tatgtatgag aaagttggtg aagcaacgga aacagcatta gttgttttat gcgaaaaaat gaatgtatat ggaacgaata aagtgggctt atcacctcgt gatctgggaa atgtttgtaa ccgagtgatt caacaaaaat ggaagaaaga atttactttg gagttttcac gtgatcggaa aagtatgagc gcattttgta taccatcatc cggtgggacg aatgcaaaaa tgtttgtgaa aggagcacca gaaggtgtac taaatcggtg tactcatgtt cgagtgaatg gacagcgtat accattaaca caaaaaataa cgcagaaaat cgttgatcag tgcattcatt atggtaccgg acgggatact ttacgatgtt tagctttggg aacaatcgat agtcctccag atcctcgcac tatgaatttg gaagattcgt cacagtttat actttacgag aaggatataa catttgtggg cgttgttggc atgttagatc caccacgagc agaagtgatt ccttcaatta aagaatgtcg tatggccggt atccgagtta tcatgatcac cggtgataac aaaaatactg cagaagctat tggtcgtcgt attggacttt ttgctgagga cgaagattct accggtaagg catttaccgg acgggaattt gacgatctac caccagagca acaaagcgat gcttgccgac gagctaaact ttttgcacgt gttgaaccca ctcataaatc aaaaattgtt gaatttcttc agtcccatgg tgaaattact gccatgactg gcgatggagt aaacgatgca ccagcactga aaaaatccga aatcggcatt gctatgggtt caggaacagc tgttgcaaaa acagcgtcag aaatggttct agctgatgat aattttgcga caattgttgc tgctgttgaa gaaggacgag ctatttacaa taacatgaag caatttattc gttatcttat ttcatcaaat attggcgagg ttgtatcgat ttttttagta gcagcattag gcatacccga agcattaatt cccgttcagc tactgtgggt gaatttagtc accgatggtc ttccggctac tgctttaggt tttaatcctc cggatctgga tattatggac cgaccaccac gttctgctgg agagtcgctt atctcgaaat ggcttttctt tcgttacatg gctgtcggat catatgttgg attagcaact gttggtgcag cgatgtggtg gtttttgttg tatgaagatg gtccacagat cagttattac caattgacac attggatgcg ttgcgaaatt gaaccagaaa actttgccga tcttgactgt gctgtttttg aggatgcaca tccgaatgca atggcacttt cagttctagt tacaattgaa atgttcaacg cactaaatag tttatcggaa aatcaatcgc ttttggtaat gccaccatgg aaaaacatct ggttgatgtc atcgatagct ttatcgctgt cattacattt tgttatctta tacgtggaaa ttctggcaac gatcttccag attacaccac ttacactagt ggaatggttc gcagtgctaa aaatttcatt ccccgttatt cttctggatg aagttttgaa atttatcgct cgaaactaca tcgatggtga gactgtttgc cgtatcggtg attatgaaaa gccagtcaag aaacgtgtgt cacataccct acttactgtt gttatgctaa ccacgctttg tgttggttac ttttattggc ttttgaaacc atattggtca caactaatgc atgcttttag tatggatcta caatcgaatt caaacagttt tcattcacat tcgcatgatg atttatga
Details for BrmaA.18982.a.HK12.GE00494
HARVESTED ON: 12/7/2015
SEQUENCED ON: 5/4/2017
EXPECTED MW: 118kDa
OBSERVED MW: 118kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MHHHHHHHHE NLYFQGGMEN AHTKSTDEVL QYFGVSGDVG LTEKQVLQNR EKYGSNELPA EEGKKLWELI LEQFDDLLVK ILLLAAIISF VLALFEEHDD QTSAVTAFVE PFVILLILIA NATVGVWQER NAESAIEALK EYEPEMAKVM REGKHGIQMI RANELVPGDI VEVSVGDKIP ADLRLIKIYS TTLRIDQSIL TGESVSVIKY TDSVPDPRAV NQDKKNCLFS GTNVAAGKAR GVVFGIGLNT EIGKIRTEMA ETETDRTPLQ QKLDEFGEQL SKVISIICVA VWAINIGHFN DPAHGGSWIK GAIYYFKIAV ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ MSVSKMFVAS KVTGDDIDFL EFTISGSTYE PSGQVFHHGR QINCASGEFE ALTELATICA MCNDSSVDYN ETKHMYEKVG EATETALVVL CEKMNVYGTN KVGLSPRDLG NVCNRVIQQK WKKEFTLEFS RDRKSMSAFC IPSSGGTNAK MFVKGAPEGV LNRCTHVRVN GQRIPLTQKI TQKIVDQCIH YGTGRDTLRC LALGTIDSPP DPRTMNLEDS SQFILYEKDI TFVGVVGMLD PPRAEVIPSI KECRMAGIRV IMITGDNKNT AEAIGRRIGL FAEDEDSTGK AFTGREFDDL PPEQQSDACR RAKLFARVEP THKSKIVEFL QSHGEITAMT GDGVNDAPAL KKSEIGIAMG SGTAVAKTAS EMVLADDNFA TIVAAVEEGR AIYNNMKQFI RYLISSNIGE VVSIFLVAAL GIPEALIPVQ LLWVNLVTDG LPATALGFNP PDLDIMDRPP RSAGESLISK WLFFRYMAVG SYVGLATVGA AMWWFLLYED GPQISYYQLT HWMRCEIEPE NFADLDCAVF EDAHPNAMAL SVLVTIEMFN ALNSLSENQS LLVMPPWKNI WLMSSIALSL SLHFVILYVE ILATIFQITP LTLVEWFAVL KISFPVILLD EVLKFIARNY IDGETVCRIG DYEKPVKKRV SHTLLTVVML TTLCVGYFYW LLKPYWSQLM HAFSMD
Full NT Sequence (Expression Vector + Insert)
aattcgccgc caccatgcat catcatcacc accatcatca cgagaatctg tatttccagg gcggtatgga aaacgctcat acgaaaagca ccgacgaagt cctgcagtac tttggtgtga gcggtgacgt tggtctgacc gaaaaacaag ttctgcagaa tcgtgagaaa tacggttcta atgaactgcc ggcggaagag ggcaaaaagc tgtgggagct gattctggag caatttgacg atctgctggt gaagattttg ctgctggcag cgatcattag cttcgttttg gcgctgtttg aggaacacga cgatcaaacc tctgcagtta cggcattcgt ggagccgttc gtcatcttgc tgattctgat cgctaacgcg accgttggcg tctggcaaga gcgtaacgca gagagcgcaa ttgaagcgct gaaagaatat gagccggaaa tggcgaaagt gatgcgtgaa ggcaaacacg gcattcagat gatccgtgcg aacgaactgg tgccgggtga catcgttgaa gtttccgtcg gtgataagat tccggccgat ttacgtctga tcaaaatcta tagcaccacc ttgcgtattg accagtccat tctgacgggt gagagcgtca gcgtcattaa gtatacggac tctgtcccgg accctcgcgc ggtgaatcaa gacaagaaaa actgcctgtt ctccggcacg aacgttgcgg caggcaaggc ccgcggtgtg gtgttcggta ttggcctgaa caccgagatc ggcaaaattc gcaccgagat ggcagaaacg gaaaccgatc gtaccccatt gcagcaaaaa ctggatgaat ttggcgaaca actgagcaag gttatttcaa tcatttgtgt ggccgtgtgg gccatcaata ttggccactt taatgacccg gcgcacggcg gcagctggat caaaggtgcg atctattact ttaagatcgc ggtggcgttg gccgtcgcgg cgatcccaga gggcctgcca gcggttatca cgacctgttt ggcgctgggt acgcgccgta tggcgaagaa aaacgcgatc gtgcgtagcc tgccgagcgt agaaacgttg ggttgcacca gcgtcatctg cagcgacaaa accggcaccc tcacgaccaa tcagatgagc gtcagcaaaa tgtttgtcgc gtccaaggtc actggtgatg acattgactt tctggagttt actatctccg gcagcaccta cgagccgagc ggtcaggttt ttcaccacgg tcgccagatc aattgcgcca gcggcgagtt tgaagctctg accgagcttg caaccatttg cgccatgtgt aacgatagct ctgttgacta taatgaaacc aagcacatgt acgaaaaagt cggtgaggcg accgagactg ccctggtggt cctgtgtgag aaaatgaacg tgtacggtac caataaagtc ggcttgagcc cgcgtgacct gggtaacgtt tgcaaccgtg tgattcagca aaaatggaaa aaagagttca cgttagagtt tagccgtgac cgtaagtcga tgagcgcctt ttgtatccct tcctcgggtg gtaccaatgc gaagatgttc gtcaagggtg ctcctgaggg tgttctcaat cgttgcaccc atgttcgtgt gaatggtcag cgcatcccgc tgacccaaaa gattactcaa aagattgtcg atcaatgcat tcactacggt acgggtcgtg acaccttgcg ttgtttggca ctgggtacga ttgattcccc gccggacccg cgtacgatga acctggaaga ttcgagccaa tttattctgt atgaaaaaga tatcaccttc gtcggcgttg tgggtatgct ggacccgcca cgtgccgaag tgattccgtc tatcaaagag tgtcgcatgg ctggtattcg tgtcatcatg attaccggcg ataacaagaa cacggcggaa gcgatcggtc gtcgtattgg tctgttcgcg gaagatgaag attctacggg caaggcattc acgggtcgcg agttcgacga tctgccgccg gagcagcaga gcgacgcatg tcgtcgcgca aagctgtttg cgcgcgtgga gccgacccac aagtctaaga tcgttgagtt cctgcaatct catggtgaga ttacggcgat gactggcgat ggcgtgaatg atgccccggc gctgaagaaa agcgagatcg gtatcgcgat gggcagcggc accgcagtgg ccaaaacggc gagcgaaatg gttctggcag atgacaactt cgcgacgatt gttgcagccg tggaagaggg ccgtgcgatc tataacaata tgaaacaatt tatccgctac ctgattagct ctaacatcgg cgaagttgtg agcattttcc tggttgcagc gcttggtatt ccggaagctc tgattcctgt tcaactgctg tgggtgaacc tggtgaccga cggtctgccg gcaaccgcgc ttggcttcaa tccgccggac ctggacatca tggatcgccc gccgcgtagc gccggtgaat ctctgatttc caagtggctg ttctttcgct acatggctgt gggtagctac gtgggcctcg ccaccgttgg tgcggcgatg tggtggttcc tgctgtatga agatggtccg cagatttctt actaccaact gacccactgg atgcgctgcg aaattgaacc ggaaaacttt gcggacctgg actgcgctgt ttttgaagat gcacacccaa atgcgatggc gctgagcgtc ctggtcacga ttgagatgtt taatgcattg aatagcttgt ccgagaatca aagcctgctg gttatgcctc cgtggaagaa catctggctg atgagcagca ttgctttgag cctgagcttg cactttgtca ttctgtacgt cgagatcctg gcaaccattt tccagatcac cccgctgact ctggtcgagt ggtttgctgt tctgaagatt tcgttcccgg tcatcctgtt ggacgaagtg ctgaagttta ttgcacgtaa ctatatcgac ggtgaaacgg tgtgtcgtat tggcgactac gagaaaccgg tcaagaaacg tgttagccac acgctgctga ccgtggttat gctgaccacc ctgtgtgtgg gctattttta ttggctgctg aaaccgtact ggtcccagct gatgcatgca tttagcatgg attgataagg ttgagcggcc gctcgaggcc ggcaaggcca gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatgtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag aattaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaagtttaa acggatctct agcg
Details for BrmaA.18982.a.HG12.PD38292
PURIFICATION DATe: 12/14/2017
CONCENTRATION: 0.05mg/ml
OBSERVED MW: 118kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 50 mM HEPES pH 7.5, 90 mM NaCl, 5% glycerol, 1 mM TCEP, 0.01% C12E9/0.005% CHS
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 9000µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGGMEN AHTKSTDEVL QYFGVSGDVG LTEKQVLQNR EKYGSNELPA EEGKKLWELI LEQFDDLLVK ILLLAAIISF VLALFEEHDD QTSAVTAFVE PFVILLILIA NATVGVWQER NAESAIEALK EYEPEMAKVM REGKHGIQMI RANELVPGDI VEVSVGDKIP ADLRLIKIYS TTLRIDQSIL TGESVSVIKY TDSVPDPRAV NQDKKNCLFS GTNVAAGKAR GVVFGIGLNT EIGKIRTEMA ETETDRTPLQ QKLDEFGEQL SKVISIICVA VWAINIGHFN DPAHGGSWIK GAIYYFKIAV ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ MSVSKMFVAS KVTGDDIDFL EFTISGSTYE PSGQVFHHGR QINCASGEFE ALTELATICA MCNDSSVDYN ETKHMYEKVG EATETALVVL CEKMNVYGTN KVGLSPRDLG NVCNRVIQQK WKKEFTLEFS RDRKSMSAFC IPSSGGTNAK MFVKGAPEGV LNRCTHVRVN GQRIPLTQKI TQKIVDQCIH YGTGRDTLRC LALGTIDSPP DPRTMNLEDS SQFILYEKDI TFVGVVGMLD PPRAEVIPSI KECRMAGIRV IMITGDNKNT AEAIGRRIGL FAEDEDSTGK AFTGREFDDL PPEQQSDACR RAKLFARVEP THKSKIVEFL QSHGEITAMT GDGVNDAPAL KKSEIGIAMG SGTAVAKTAS EMVLADDNFA TIVAAVEEGR AIYNNMKQFI RYLISSNIGE VVSIFLVAAL GIPEALIPVQ LLWVNLVTDG LPATALGFNP PDLDIMDRPP RSAGESLISK WLFFRYMAVG SYVGLATVGA AMWWFLLYED GPQISYYQLT HWMRCEIEPE NFADLDCAVF EDAHPNAMAL SVLVTIEMFN ALNSLSENQS LLVMPPWKNI WLMSSIALSL SLHFVILYVE ILATIFQITP LTLVEWFAVL KISFPVILLD EVLKFIARNY IDGETVCRIG DYEKPVKKRV SHTLLTVVML TTLCVGYFYW LLKPYWSQLM HAFSMD
Full NT Sequence (Expression Vector + Insert)
AATTCGCCGC CACCATGCAT CATCATCACC ACCATCATCA CGAGAATCTG TATTTCCAGG GCGGTATGGA AAACGCTCAT ACGAAAAGCA CCGACGAAGT CCTGCAGTAC TTTGGTGTGA GCGGTGACGT TGGTCTGACC GAAAAACAAG TTCTGCAGAA TCGTGAGAAA TACGGTTCTA ATGAACTGCC GGCGGAAGAG GGCAAAAAGC TGTGGGAGCT GATTCTGGAG CAATTTGACG ATCTGCTGGT GAAGATTTTG CTGCTGGCAG CGATCATTAG CTTCGTTTTG GCGCTGTTTG AGGAACACGA CGATCAAACC TCTGCAGTTA CGGCATTCGT GGAGCCGTTC GTCATCTTGC TGATTCTGAT CGCTAACGCG ACCGTTGGCG TCTGGCAAGA GCGTAACGCA GAGAGCGCAA TTGAAGCGCT GAAAGAATAT GAGCCGGAAA TGGCGAAAGT GATGCGTGAA GGCAAACACG GCATTCAGAT GATCCGTGCG AACGAACTGG TGCCGGGTGA CATCGTTGAA GTTTCCGTCG GTGATAAGAT TCCGGCCGAT TTACGTCTGA TCAAAATCTA TAGCACCACC TTGCGTATTG ACCAGTCCAT TCTGACGGGT GAGAGCGTCA GCGTCATTAA GTATACGGAC TCTGTCCCGG ACCCTCGCGC GGTGAATCAA GACAAGAAAA ACTGCCTGTT CTCCGGCACG AACGTTGCGG CAGGCAAGGC CCGCGGTGTG GTGTTCGGTA TTGGCCTGAA CACCGAGATC GGCAAAATTC GCACCGAGAT GGCAGAAACG GAAACCGATC GTACCCCATT GCAGCAAAAA CTGGATGAAT TTGGCGAACA ACTGAGCAAG GTTATTTCAA TCATTTGTGT GGCCGTGTGG GCCATCAATA TTGGCCACTT TAATGACCCG GCGCACGGCG GCAGCTGGAT CAAAGGTGCG ATCTATTACT TTAAGATCGC GGTGGCGTTG GCCGTCGCGG CGATCCCAGA GGGCCTGCCA GCGGTTATCA CGACCTGTTT GGCGCTGGGT ACGCGCCGTA TGGCGAAGAA AAACGCGATC GTGCGTAGCC TGCCGAGCGT AGAAACGTTG GGTTGCACCA GCGTCATCTG CAGCGACAAA ACCGGCACCC TCACGACCAA TCAGATGAGC GTCAGCAAAA TGTTTGTCGC GTCCAAGGTC ACTGGTGATG ACATTGACTT TCTGGAGTTT ACTATCTCCG GCAGCACCTA CGAGCCGAGC GGTCAGGTTT TTCACCACGG TCGCCAGATC AATTGCGCCA GCGGCGAGTT TGAAGCTCTG ACCGAGCTTG CAACCATTTG CGCCATGTGT AACGATAGCT CTGTTGACTA TAATGAAACC AAGCACATGT ACGAAAAAGT CGGTGAGGCG ACCGAGACTG CCCTGGTGGT CCTGTGTGAG AAAATGAACG TGTACGGTAC CAATAAAGTC GGCTTGAGCC CGCGTGACCT GGGTAACGTT TGCAACCGTG TGATTCAGCA AAAATGGAAA AAAGAGTTCA CGTTAGAGTT TAGCCGTGAC CGTAAGTCGA TGAGCGCCTT TTGTATCCCT TCCTCGGGTG GTACCAATGC GAAGATGTTC GTCAAGGGTG CTCCTGAGGG TGTTCTCAAT CGTTGCACCC ATGTTCGTGT GAATGGTCAG CGCATCCCGC TGACCCAAAA GATTACTCAA AAGATTGTCG ATCAATGCAT TCACTACGGT ACGGGTCGTG ACACCTTGCG TTGTTTGGCA CTGGGTACGA TTGATTCCCC GCCGGACCCG CGTACGATGA ACCTGGAAGA TTCGAGCCAA TTTATTCTGT ATGAAAAAGA TATCACCTTC GTCGGCGTTG TGGGTATGCT GGACCCGCCA CGTGCCGAAG TGATTCCGTC TATCAAAGAG TGTCGCATGG CTGGTATTCG TGTCATCATG ATTACCGGCG ATAACAAGAA CACGGCGGAA GCGATCGGTC GTCGTATTGG TCTGTTCGCG GAAGATGAAG ATTCTACGGG CAAGGCATTC ACGGGTCGCG AGTTCGACGA TCTGCCGCCG GAGCAGCAGA GCGACGCATG TCGTCGCGCA AAGCTGTTTG CGCGCGTGGA GCCGACCCAC AAGTCTAAGA TCGTTGAGTT CCTGCAATCT CATGGTGAGA TTACGGCGAT GACTGGCGAT GGCGTGAATG ATGCCCCGGC GCTGAAGAAA AGCGAGATCG GTATCGCGAT GGGCAGCGGC ACCGCAGTGG CCAAAACGGC GAGCGAAATG GTTCTGGCAG ATGACAACTT CGCGACGATT GTTGCAGCCG TGGAAGAGGG CCGTGCGATC TATAACAATA TGAAACAATT TATCCGCTAC CTGATTAGCT CTAACATCGG CGAAGTTGTG AGCATTTTCC TGGTTGCAGC GCTTGGTATT CCGGAAGCTC TGATTCCTGT TCAACTGCTG TGGGTGAACC TGGTGACCGA CGGTCTGCCG GCAACCGCGC TTGGCTTCAA TCCGCCGGAC CTGGACATCA TGGATCGCCC GCCGCGTAGC GCCGGTGAAT CTCTGATTTC CAAGTGGCTG TTCTTTCGCT ACATGGCTGT GGGTAGCTAC GTGGGCCTCG CCACCGTTGG TGCGGCGATG TGGTGGTTCC TGCTGTATGA AGATGGTCCG CAGATTTCTT ACTACCAACT GACCCACTGG ATGCGCTGCG AAATTGAACC GGAAAACTTT GCGGACCTGG ACTGCGCTGT TTTTGAAGAT GCACACCCAA ATGCGATGGC GCTGAGCGTC CTGGTCACGA TTGAGATGTT TAATGCATTG AATAGCTTGT CCGAGAATCA AAGCCTGCTG GTTATGCCTC CGTGGAAGAA CATCTGGCTG ATGAGCAGCA TTGCTTTGAG CCTGAGCTTG CACTTTGTCA TTCTGTACGT CGAGATCCTG GCAACCATTT TCCAGATCAC CCCGCTGACT CTGGTCGAGT GGTTTGCTGT TCTGAAGATT TCGTTCCCGG TCATCCTGTT GGACGAAGTG CTGAAGTTTA TTGCACGTAA CTATATCGAC GGTGAAACGG TGTGTCGTAT TGGCGACTAC GAGAAACCGG TCAAGAAACG TGTTAGCCAC ACGCTGCTGA CCGTGGTTAT GCTGACCACC CTGTGTGTGG GCTATTTTTA TTGGCTGCTG AAACCGTACT GGTCCCAGCT GATGCATGCA TTTAGCATGG ATTGATAAGG TTGAGCGGCC GCTCGAGGCC GGCAAGGCCA GATCCCCCGA CCTCGACCTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTGGTCGAG ATCCCTCGGA GATCTCTAGC TAGAGGATCG ATCCCCGCCC CGGACGAACT AAACCTGACT ACGACATCTC TGCCCCTTCT TCGCGGGGCA GTGCATGTAA TCCCTTCAGT TGGTTGGTAC AACTTGCCAA CTGAACCCTA AACGGGTAGC ATATGCTTCC CGGGTAGTAG TATATACTAT CCAGACTAAC CCTAATTCAA TAGCATATGT TACCCAACGG GAAGCATATG CTATCGAATT AGGGTTAGTA AAAGGGTCCT AAGGAACAGC GATGTAGGTG GGCGGGCCAA GATAGGGGCG CGATTGCTGC GATCTGGAGG ACAAATTACA CACACTTGCG CCTGAGCGCC AAGCACAGGG TTGTTGGTCC TCATATTCAC GAGGTCGCTG AGAGCACGGT GGGCTAATGT TGCCATGGGT AGCATATACT ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA TATGCTATCC TAATAGAGAT TAGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTCAT GATAAGCTGT CAAACATGAG AATTAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCG
Details for BrmaA.18982.a.HG12.PD38293
PURIFICATION DATe: 12/14/2017
CONCENTRATION: 0.03mg/ml
OBSERVED MW: 118kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: REQUIRED50 mM HEPES pH 7.5, 90 mM NaCl, 5% glycerol, 1 mM TCEP, 0.01% C12E9/0.005% CHS
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 9000µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MHHHHHHHHE NLYFQGGMEN AHTKSTDEVL QYFGVSGDVG LTEKQVLQNR EKYGSNELPA EEGKKLWELI LEQFDDLLVK ILLLAAIISF VLALFEEHDD QTSAVTAFVE PFVILLILIA NATVGVWQER NAESAIEALK EYEPEMAKVM REGKHGIQMI RANELVPGDI VEVSVGDKIP ADLRLIKIYS TTLRIDQSIL TGESVSVIKY TDSVPDPRAV NQDKKNCLFS GTNVAAGKAR GVVFGIGLNT EIGKIRTEMA ETETDRTPLQ QKLDEFGEQL SKVISIICVA VWAINIGHFN DPAHGGSWIK GAIYYFKIAV ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ MSVSKMFVAS KVTGDDIDFL EFTISGSTYE PSGQVFHHGR QINCASGEFE ALTELATICA MCNDSSVDYN ETKHMYEKVG EATETALVVL CEKMNVYGTN KVGLSPRDLG NVCNRVIQQK WKKEFTLEFS RDRKSMSAFC IPSSGGTNAK MFVKGAPEGV LNRCTHVRVN GQRIPLTQKI TQKIVDQCIH YGTGRDTLRC LALGTIDSPP DPRTMNLEDS SQFILYEKDI TFVGVVGMLD PPRAEVIPSI KECRMAGIRV IMITGDNKNT AEAIGRRIGL FAEDEDSTGK AFTGREFDDL PPEQQSDACR RAKLFARVEP THKSKIVEFL QSHGEITAMT GDGVNDAPAL KKSEIGIAMG SGTAVAKTAS EMVLADDNFA TIVAAVEEGR AIYNNMKQFI RYLISSNIGE VVSIFLVAAL GIPEALIPVQ LLWVNLVTDG LPATALGFNP PDLDIMDRPP RSAGESLISK WLFFRYMAVG SYVGLATVGA AMWWFLLYED GPQISYYQLT HWMRCEIEPE NFADLDCAVF EDAHPNAMAL SVLVTIEMFN ALNSLSENQS LLVMPPWKNI WLMSSIALSL SLHFVILYVE ILATIFQITP LTLVEWFAVL KISFPVILLD EVLKFIARNY IDGETVCRIG DYEKPVKKRV SHTLLTVVML TTLCVGYFYW LLKPYWSQLM HAFSMD
Full NT Sequence (Expression Vector + Insert)
AATTCGCCGC CACCATGCAT CATCATCACC ACCATCATCA CGAGAATCTG TATTTCCAGG GCGGTATGGA AAACGCTCAT ACGAAAAGCA CCGACGAAGT CCTGCAGTAC TTTGGTGTGA GCGGTGACGT TGGTCTGACC GAAAAACAAG TTCTGCAGAA TCGTGAGAAA TACGGTTCTA ATGAACTGCC GGCGGAAGAG GGCAAAAAGC TGTGGGAGCT GATTCTGGAG CAATTTGACG ATCTGCTGGT GAAGATTTTG CTGCTGGCAG CGATCATTAG CTTCGTTTTG GCGCTGTTTG AGGAACACGA CGATCAAACC TCTGCAGTTA CGGCATTCGT GGAGCCGTTC GTCATCTTGC TGATTCTGAT CGCTAACGCG ACCGTTGGCG TCTGGCAAGA GCGTAACGCA GAGAGCGCAA TTGAAGCGCT GAAAGAATAT GAGCCGGAAA TGGCGAAAGT GATGCGTGAA GGCAAACACG GCATTCAGAT GATCCGTGCG AACGAACTGG TGCCGGGTGA CATCGTTGAA GTTTCCGTCG GTGATAAGAT TCCGGCCGAT TTACGTCTGA TCAAAATCTA TAGCACCACC TTGCGTATTG ACCAGTCCAT TCTGACGGGT GAGAGCGTCA GCGTCATTAA GTATACGGAC TCTGTCCCGG ACCCTCGCGC GGTGAATCAA GACAAGAAAA ACTGCCTGTT CTCCGGCACG AACGTTGCGG CAGGCAAGGC CCGCGGTGTG GTGTTCGGTA TTGGCCTGAA CACCGAGATC GGCAAAATTC GCACCGAGAT GGCAGAAACG GAAACCGATC GTACCCCATT GCAGCAAAAA CTGGATGAAT TTGGCGAACA ACTGAGCAAG GTTATTTCAA TCATTTGTGT GGCCGTGTGG GCCATCAATA TTGGCCACTT TAATGACCCG GCGCACGGCG GCAGCTGGAT CAAAGGTGCG ATCTATTACT TTAAGATCGC GGTGGCGTTG GCCGTCGCGG CGATCCCAGA GGGCCTGCCA GCGGTTATCA CGACCTGTTT GGCGCTGGGT ACGCGCCGTA TGGCGAAGAA AAACGCGATC GTGCGTAGCC TGCCGAGCGT AGAAACGTTG GGTTGCACCA GCGTCATCTG CAGCGACAAA ACCGGCACCC TCACGACCAA TCAGATGAGC GTCAGCAAAA TGTTTGTCGC GTCCAAGGTC ACTGGTGATG ACATTGACTT TCTGGAGTTT ACTATCTCCG GCAGCACCTA CGAGCCGAGC GGTCAGGTTT TTCACCACGG TCGCCAGATC AATTGCGCCA GCGGCGAGTT TGAAGCTCTG ACCGAGCTTG CAACCATTTG CGCCATGTGT AACGATAGCT CTGTTGACTA TAATGAAACC AAGCACATGT ACGAAAAAGT CGGTGAGGCG ACCGAGACTG CCCTGGTGGT CCTGTGTGAG AAAATGAACG TGTACGGTAC CAATAAAGTC GGCTTGAGCC CGCGTGACCT GGGTAACGTT TGCAACCGTG TGATTCAGCA AAAATGGAAA AAAGAGTTCA CGTTAGAGTT TAGCCGTGAC CGTAAGTCGA TGAGCGCCTT TTGTATCCCT TCCTCGGGTG GTACCAATGC GAAGATGTTC GTCAAGGGTG CTCCTGAGGG TGTTCTCAAT CGTTGCACCC ATGTTCGTGT GAATGGTCAG CGCATCCCGC TGACCCAAAA GATTACTCAA AAGATTGTCG ATCAATGCAT TCACTACGGT ACGGGTCGTG ACACCTTGCG TTGTTTGGCA CTGGGTACGA TTGATTCCCC GCCGGACCCG CGTACGATGA ACCTGGAAGA TTCGAGCCAA TTTATTCTGT ATGAAAAAGA TATCACCTTC GTCGGCGTTG TGGGTATGCT GGACCCGCCA CGTGCCGAAG TGATTCCGTC TATCAAAGAG TGTCGCATGG CTGGTATTCG TGTCATCATG ATTACCGGCG ATAACAAGAA CACGGCGGAA GCGATCGGTC GTCGTATTGG TCTGTTCGCG GAAGATGAAG ATTCTACGGG CAAGGCATTC ACGGGTCGCG AGTTCGACGA TCTGCCGCCG GAGCAGCAGA GCGACGCATG TCGTCGCGCA AAGCTGTTTG CGCGCGTGGA GCCGACCCAC AAGTCTAAGA TCGTTGAGTT CCTGCAATCT CATGGTGAGA TTACGGCGAT GACTGGCGAT GGCGTGAATG ATGCCCCGGC GCTGAAGAAA AGCGAGATCG GTATCGCGAT GGGCAGCGGC ACCGCAGTGG CCAAAACGGC GAGCGAAATG GTTCTGGCAG ATGACAACTT CGCGACGATT GTTGCAGCCG TGGAAGAGGG CCGTGCGATC TATAACAATA TGAAACAATT TATCCGCTAC CTGATTAGCT CTAACATCGG CGAAGTTGTG AGCATTTTCC TGGTTGCAGC GCTTGGTATT CCGGAAGCTC TGATTCCTGT TCAACTGCTG TGGGTGAACC TGGTGACCGA CGGTCTGCCG GCAACCGCGC TTGGCTTCAA TCCGCCGGAC CTGGACATCA TGGATCGCCC GCCGCGTAGC GCCGGTGAAT CTCTGATTTC CAAGTGGCTG TTCTTTCGCT ACATGGCTGT GGGTAGCTAC GTGGGCCTCG CCACCGTTGG TGCGGCGATG TGGTGGTTCC TGCTGTATGA AGATGGTCCG CAGATTTCTT ACTACCAACT GACCCACTGG ATGCGCTGCG AAATTGAACC GGAAAACTTT GCGGACCTGG ACTGCGCTGT TTTTGAAGAT GCACACCCAA ATGCGATGGC GCTGAGCGTC CTGGTCACGA TTGAGATGTT TAATGCATTG AATAGCTTGT CCGAGAATCA AAGCCTGCTG GTTATGCCTC CGTGGAAGAA CATCTGGCTG ATGAGCAGCA TTGCTTTGAG CCTGAGCTTG CACTTTGTCA TTCTGTACGT CGAGATCCTG GCAACCATTT TCCAGATCAC CCCGCTGACT CTGGTCGAGT GGTTTGCTGT TCTGAAGATT TCGTTCCCGG TCATCCTGTT GGACGAAGTG CTGAAGTTTA TTGCACGTAA CTATATCGAC GGTGAAACGG TGTGTCGTAT TGGCGACTAC GAGAAACCGG TCAAGAAACG TGTTAGCCAC ACGCTGCTGA CCGTGGTTAT GCTGACCACC CTGTGTGTGG GCTATTTTTA TTGGCTGCTG AAACCGTACT GGTCCCAGCT GATGCATGCA TTTAGCATGG ATTGATAAGG TTGAGCGGCC GCTCGAGGCC GGCAAGGCCA GATCCCCCGA CCTCGACCTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTGGTCGAG ATCCCTCGGA GATCTCTAGC TAGAGGATCG ATCCCCGCCC CGGACGAACT AAACCTGACT ACGACATCTC TGCCCCTTCT TCGCGGGGCA GTGCATGTAA TCCCTTCAGT TGGTTGGTAC AACTTGCCAA CTGAACCCTA AACGGGTAGC ATATGCTTCC CGGGTAGTAG TATATACTAT CCAGACTAAC CCTAATTCAA TAGCATATGT TACCCAACGG GAAGCATATG CTATCGAATT AGGGTTAGTA AAAGGGTCCT AAGGAACAGC GATGTAGGTG GGCGGGCCAA GATAGGGGCG CGATTGCTGC GATCTGGAGG ACAAATTACA CACACTTGCG CCTGAGCGCC AAGCACAGGG TTGTTGGTCC TCATATTCAC GAGGTCGCTG AGAGCACGGT GGGCTAATGT TGCCATGGGT AGCATATACT ACCCAAATAT CTGGATAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA TATGCTATCC TAATAGAGAT TAGGGTAGTA TATGCTATCC TAATTTATAT CTGGGTAGCA TATACTACCC AAATATCTGG ATAGCATATG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT TTATATCTGG GTAGCATAGG CTATCCTAAT CTATATCTGG GTAGCATATG CTATCCTAAT CTATATCTGG GTAGTATATG CTATCCTAAT CTGTATCCGG GTAGCATATG CTATCCTCAT GATAAGCTGT CAAACATGAG AATTAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GTACATTTAT ATTGGCTCAT GTCCAATATG ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TCCGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTACGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACACCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAATA ACCCCGCCCC GTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCCTCA CTCTCTTCCG CATCGCTGTC TGCGAGGGCC AGCTGTTGGG CTCGCGGTTG AGGACAAACT CTTCGCGGTC TTTCCAGTAC TCTTGGATCG GAAACCCGTC GGCCTCCGAA CGGTACTCCG CCACCGAGGG ACCTGAGCGA GTCCGCATCG ACCGGATCGG AAAACCTCTC GAGAAAGGCG TCTAACCAGT CACAGTCGCA AGGTAGGCTG AGCACCGTGG CGGGCGGCAG CGGGTGGCGG TCGGGGTTGT TTCTGGCGGA GGTGCTGCTG ATGATGTAAT TAAAGTAGGC GGTCTTGAGA CGGCGGATGG TCGAGGTGAG GTGTGGCAGG CTTGAGATCC AGCTGTTGGG GTGAGTACTC CCTCTCAAAA GCGGGCATTA CTTCTGCGCT AAGATTGTCA GTTTCCAAAA ACGAGGAGGA TTTGATATTC ACCTGGCCCG ATCTGGCCAT ACACTTGAGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGGT CCAAGTTTAA ACGGATCTCT AGCG