SSGCID: Target Page

BrmaA.18889.c

BMA-AARS-1, isoform e; alanyl-tRNA synthetase (AlaRS), putative

CENTER ID:	BrmaA.18889.c
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.18889.c.B1.GE41317	full length		1	293

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0J9XR05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRRDEFQGL TWLIPWVDVM ELIEIGDVFV MNKLWSNTAY YIWATSCLRV HIRRLSAQQI RLSFLEYFKE HNHTYVPSSS VIPEDDSSVT FVNAGMNQFK PLFLGAKYTE GKLAALRNVV NWQKCIRIGG KHNDFDDVGR DLTHHTFFEM LGNYSFGGYS KMEACLYAWN FLTDVLKIPA DRLYITYFGG DESMKLKEDR ECRDIWIKLG VPEDRVLGFC SNHNFWEMAQ TGPCGPCSEI HYDLIGNRKA QKLVNSSNPT VVEIWNLVFM QFSRLRNGFR TISEYCPRIT LCL

NT Sequence

atggatagac gtgatgaatt ccagggattg acgtggctga ttccgtgggt tgacgtgatg gagttgattg aaattggtga tgtattcgtg atgaataaat tatggtcgaa tactgcatat tacatctggg ccacttcatg tttgcgtgtg catatacgtc gtttatcggc acaacagatt cgtttatctt ttttagagta cttcaaagaa cataatcata catatgtgcc ttcttcatct gttattccag aagatgatag ctccgttaca tttgttaatg cggggatgaa tcagttcaag ccactttttc tgggtgctaa atatacagaa ggcaaattgg ccgctttacg taatgttgtt aactggcaaa agtgtattcg tattggtgga aagcataatg atttcgacga tgttggccgt gatttgacac atcatacatt tttcgaaatg cttggaaact attcatttgg tggctactca aagatggaag catgtttata tgcatggaac ttcctcacag acgtcctcaa gataccagct gatcgcttat atattaccta tttcggtggg gatgaaagca tgaagttaaa ggaagatcgt gaatgccgcg atatttggat taaattaggt gtccctgaag atcgtgttct tggcttttgt tccaatcata atttctggga gatggctcag acgggtccat gtggtccttg ttcggaaatt cattatgatc ttattggtaa tcgaaaggca cagaaacttg ttaattcaag taatccaaca gtagtcgaaa tttggaatct tgtttttatg cagttcagta gattgcggaa tgggtttcga acgattagtg agtattgtcc aaggattaca ctctgcctat ga

Details for BrmaA.18889.c.B1.GE41317

HARVESTED ON:	3/31/2017
SEQUENCED ON:	4/10/2017
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	94
PERCENT COVERAGE:	105

Validated AA Sequence

MAHHHHHHMD RRDEFQGLTW LIPWVDVMEL IEIGDVFVMN KLWSNTAYYI WATSCLRVHI RRLSAQQIRL SFLEYFKEHN HTYVPSSSVI PEDDSSVTFV NAGMNQFKPL FLGAKYTEGK LAALRNVVNW QKCIRIGGKH NDFDDVGRDL THHTFFEMLG NYSFGGYSKM EACLYAWNFL TDVLKIPADR LYITYFGGDE SMKLKEDREC RDIWIKLGVP EDRVLGFCSN HNFWEMAQTG PCGPCSEIHY DLIGNRKAQK LVNSSNPTVV EIWNLVFMQF SRDISGKISS LPTLYIDCGM GFERLVSXXX XLHSAYK

Validated NT Sequence

atcttactca cttataggca gagtgtaatc nnnnnnnnnn actcactaat cgttcgaaac ccattccgca atctatgtac aacgttggta aactactgat tttgcctgat atatccctac tgaactgcat aaaaacaaga ttccaaattt cgactactgt tggattactt gaattaacaa gtttctgtgc ctttcgatta ccaataagat cataatgaat ttccgaacaa ggaccacatg gacccgtctg agccatctcc cagaaattat gattggaaca aaagccaaga acacgatctt cagggacacc taatttaatc caaatatcgc ggcattcacg atcttccttt aacttcatgc tttcatcccc accgaaatag gtaatatata agcgatcagc tggtatcttg aggacgtctg tgaggaagtt ccatgcatat aaacatgctt ccatctttga gtagccacca aatgaatagt ttccaagcat ttcgaaaaat gtatgatgtg tcaaatcacg gccaacatcg tcgaaatcat tatgctttcc accaatacga atacactttt gccagttaac aacattacgt aaagcggcca atttgccttc tgtatattta gcacccagaa aaagtggctt gaactgattc atccccgcat taacaaatgt aacggagcta tcatcttctg gaataacaga tgaagaaggc acatatgtat gattatgttc tttgaagtac tctaaaaaag ataaacgaat ctgttgtgcc gataaacgac gtatatgcac acgcaaacat gaagtggccc agatgtaata tgcagtattc gaccataatt tattcatcac gaatacatca ccaatttcaa tcaactccat cacgtcaacc cacggaatca gccacgtcaa tccctggaat tcatcacgtc tatccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata gacgtgatga attccaggga ttgacgtggc tgattccgtg ggttgacgtg atggagttga ttgaaattgg tgatgtattc gtgatgaata aattatggtc gaatactgca tattacatct gggccacttc atgtttgcgt gtgcatatac gtcgtttatc ggcacaacag attcgtttat cttttttaga gtacttcaaa gaacataatc atacatatgt gccttcttca tctgttattc cagaagatga tagctccgtt acatttgtta atgcggggat gaatcagttc aagccacttt ttctgggtgc taaatataca gaaggcaaat tggccgcttt acgtaatgtt gttaactggc aaaagtgtat tcgtattggt ggaaagcata atgatttcga cgatgttggc cgtgatttga cacatcatac atttttcgaa atgcttggaa actattcatt tggtggctac tcaaagatgg aagcatgttt atatgcatgg aacttcctca cagacgtcct caagatacca gctgatcgct tatatattac ctatttcggt ggggatgaaa gcatgaagtt aaaggaagat cgtgaatgcc gcgatatttg gattaaatta ggtgtccctg aagatcgtgt tcttggcttt tgttccaatc ataatttctg ggagatggct cagacgggtc catgtggtcc ttgttcggaa attcattatg atcttattgg taatcgaaag gcacagaaac ttgttaattc aagtaatcca acagtagtcg aaatttggaa tcttgttttt atgcagttca gtagattgcg gaatgggttt cgaacgatta gtgagtattg tccaaggatt acactctgcc tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat