SSGCID: Target Page

BrmaA.18889.b

BMA-AARS-1, isoform b; alanyl-tRNA synthetase (AlaRS), putative

CENTER ID:	BrmaA.18889.b
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.18889.b.B1.GE41316	full length		1	833

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0J9XRL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNKLWSNTAY YIWATSCLRV HIRRLSAQQI RLSFLEYFKE HNHTYVPSSS VIPEDDSSVT FVNAGMNQFK PLFLGAKYTE GKLAALRNVV NWQKCIRIGG KHNDFDDVGR DLTHHTFFEM LGNYSFGGYS KMEACLYAWN FLTDVLKIPA DRLYITYFGG DESMKLKEDR ECRDIWIKLG VPEDRVLGFC SNHNFWEMAQ TGPCGPCSEI HYDLIGNRKA QKLVNSSNPT VVEIWNLVFM QFSRDISGKI SSLPTLYIDC GMGFERLVSI VQGLHSAYDT DLFLPLMRII HEYSKVRGYG GQLGDIDTAY RIVADHLRAA CIMISDGVEP SSRNRGYHLR RVLRRAALNF TLTLGAERGM LASLVPDFVN HITLLYNNVA ACETVIAKTV MSEEQLFWRS YDKGCKLLEH NIASQQHVLS GEIAWMLSGT YGLPLSITQK ICREKGLKVD VDGFQQCLAN FQKAQKAEEE LWQKIDLEEM ILNGVEPTND SEKYCCERIE LGKYEFPSRT GTVVAIFDVN GKNVMSLNPG ELGSVVMDST IFFAEQGGQL YDRGILQDNL NNTIFIVNSV KRRNGYIIHT GKVADNGILE KGVNLTQIID PKQRFFLMCG HTATHILHFA LEKVFGVSVR QMGSFIGPDK LHFDFFIPDQ KIALEKIEKV MAMVNNIIES NINVTVKYIL QEELGSLHKD FSAFDISQEA SSNGSYRIIT VESSALLGQD IIEPCCGTHV LNTSDIGRFV IIGQKSRGAN VRRIYAVTNS AALESIHNAT KLKDELSDAL SNVSGTFIDT QRLLKDFQKN LPLPSKSSIY EMLKIAKKRN KCLSQKLRLP DAT

NT Sequence

atgaataaat tatggtcgaa tactgcatat tacatctggg ccacttcatg tttgcgtgtg catatacgtc gtttatcggc acaacagatt cgtttatctt ttttagagta cttcaaagaa cataatcata catatgtgcc ttcttcatct gttattccag aagatgatag ctccgttaca tttgttaatg cggggatgaa tcagttcaag ccactttttc tgggtgctaa atatacagaa ggcaaattgg ccgctttacg taatgttgtt aactggcaaa agtgtattcg tattggtgga aagcataatg atttcgacga tgttggccgt gatttgacac atcatacatt tttcgaaatg cttggaaact attcatttgg tggctactca aagatggaag catgtttata tgcatggaac ttcctcacag acgtcctcaa gataccagct gatcgcttat atattaccta tttcggtggg gatgaaagca tgaagttaaa ggaagatcgt gaatgccgcg atatttggat taaattaggt gtccctgaag atcgtgttct tggcttttgt tccaatcata atttctggga gatggctcag acgggtccat gtggtccttg ttcggaaatt cattatgatc ttattggtaa tcgaaaggca cagaaacttg ttaattcaag taatccaaca gtagtcgaaa tttggaatct tgtttttatg cagttcagta gggatatatc aggcaaaatc agtagtttac caacgttgta catagattgc ggaatgggtt tcgaacgatt agtgagtatt gtccaaggat tacactctgc ctatgatacc gacttatttc ttccattaat gagaattatt cacgagtatt cgaaagttag aggatatggt ggacaactgg gggatatcga cacagcatat cgaattgtcg cggatcatct tcgtgctgct tgtattatga tttcagacgg tgtcgaacct agttcacgaa atcgagggta tcatcttcga cgggttttgc gacgtgctgc attgaatttt accctcacgc ttggtgcaga acgcgggatg ttagcatctt tagtgcctga ttttgttaat catataactt tactgtacaa caatgtcgca gcttgtgaaa ctgtaatagc taaaacggtt atgtctgagg aacagttgtt ttggagaagt tatgacaaag gctgtaaatt acttgagcat aatattgcat ctcagcaaca tgtgctgtca ggagaaattg cgtggatgtt gtcaggaact tacggtcttc cattgtcaat aacccaaaag atatgtagag aaaaagggtt gaaagttgat gtagatggct tccaacaatg tctggctaat tttcaaaaag ctcaaaaagc tgaagaggag ctttggcaaa aaatcgattt agaagaaatg atactgaatg gtgtagagcc aacgaatgat tcagaaaaat attgttgtga acggattgaa ttgggcaagt atgaattccc gagcaggaca ggtactgttg tggcaatctt tgatgtcaat ggtaaaaatg taatgtcgct taatccaggc gaattagggt ctgtcgttat ggattcgact attttttttg ctgaacaagg agggcaattg tacgacagag gcatccttca agataattta aataatacta tatttattgt gaatagtgtg aaaagacgaa atggctacat cattcataca gggaaagtag cagataatgg aattttggaa aaaggcgtaa acttgacgca gattatagat cctaagcaac ggtttttttt aatgtgtggt catactgcta cccatattct tcattttgct ctcgaaaaag tatttggtgt atcagtccgt cagatgggtt catttattgg ccctgataag ttacattttg atttctttat tcctgaccaa aaaatcgctc ttgaaaagat cgaaaaagta atggcaatgg tgaataatat tattgaaagt aacataaacg ttacggtgaa atacattttg caagaagaat taggttctct gcataaggat ttttctgcat tcgacatttc acaagaggca tcctccaacg gctcatatcg gatcattaca gttgagagtt cggcattact cggtcaagat atcatcgaac catgttgtgg gacgcatgtt ttaaacacaa gtgatattgg tcgatttgtt ataattggtc agaaatcacg aggtgccaat gtacgtcgaa tttacgcagt aacaaatagc gctgctctgg aaagcattca taatgcgacg aagcttaaag atgaactatc tgatgcgttg agcaatgtta gcggaacctt catcgatacg cagaggctgt tgaaagattt tcaaaagaat cttccattgc catccaaatc atcaatatat gaaatgctta aaatagcgaa gaagagaaac aagtgcctaa gtcagaaact tcgtttacct gatgcaactt aa

Details for BrmaA.18889.b.B1.GE41316

HARVESTED ON:	3/31/2017
SEQUENCED ON:	4/10/2017
EXPECTED MW:	95kDa
OBSERVED MW:	95kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN KLWSNTAYYI WATSCLRVHI RRLSAQQIRL SFLEYFKEHN HTYVPSSSVI PEDDSSVTFV NAGMNQFKPL FLGAKYTEGK LAALRNVVNW QKCIRIGGKH NDFDDVGRDL THHTFFEMLG NYSFGGYSKM EACLYAWNFL TDVLKIPADR LYITYFGGDE SMKLKEDREC RDIWIKLGVP EDRVLGFCSN HNFWEMAQTG PCGPCSEIHY DLIGNRKAQK LVNSSNPTVV EIWNLVFMQF SRDISGKISS LPTLYIDCGM GFERLVSIVQ GLHSAYDTDL FLPLMRIIHE YSKVRGYGGQ LGDIDTAYRI VADHLRAACI MISDGVEPSS RNRGYHLRRV LRRAALNFTL TLGAERGMLA SLVPDFVNHI TLLYNNVAAC ETVIAKTVMS EEQLFWRSYD KGCKLLEHNI ASQQHVLSGE IAWMLSGTYG LPLSITQKIC REKGLKVDVD GFQQCLANFQ KAQKAEEELW QKIDLEEMIL NGVEPTNDSE KYCCERIELG KYEFPSRTGT VVAIFDVNGK NVMSLNPGEL GSVVMDSTIF FAEQGGQLYD RGILQDNLNN TIFIVNSVKR RNGYIIHTGK VADNGILEKG VNLTQIIDPK QRFFLMCGHT ATHILHFALE KVFGVSVRQM GSFIGPDKLH FDFFIPDQKI ALEKIEKVMA MVNNIIESNI NVTVKYILQE ELGSLHKDFS AFDISQEASS NGSYRIITVE SSALLGQDII EPCCGTHVLN TSDIGRFVII GQKSRGANVR RIYAVTNSAA LESIHNATKL KDELSDALSN VSGTFIDTQR LLKDFQKNLP LPSKSSIYEM LKIAKKRNKC LSQKLRLPDA T

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata aattatggtc gaatactgca tattacatct gggccacttc atgtttgcgt gtgcatatac gtcgtttatc ggcacaacag attcgtttat cttttttaga gtacttcaaa gaacataatc atacatatgt gccttcttca tctgttattc cagaagatga tagctccgtt acatttgtta atgcggggat gaatcagttc aagccacttt ttctgggtgc taaatataca gaaggcaaat tggccgcttt acgtaatgtt gttaactggc aaaagtgtat tcgtattggt ggaaagcata atgatttcga cgatgttggc cgtgatttga cacatcatac atttttcgaa atgcttggaa actattcatt tggtggctac tcaaagatgg aagcatgttt atatgcatgg aacttcctca cagacgtcct caagatacca gctgatcgct tatatattac ctatttcggt ggggatgaaa gcatgaagtt aaaggaagat cgtgaatgcc gcgatatttg gattaaatta ggtgtccctg aagatcgtgt tcttggcttt tgttccaatc ataatttctg ggagatggct cagacgggtc catgtggtcc ttgttcggaa attcattatg atcttattgg taatcgaaag gcacagaaac ttgttaattc aagtaatcca acagtagtcg aaatttggaa tcttgttttt atgcagttca gtagggatat atcaggcaaa atcagtagtt taccaacgtt gtacatagat tgcggaatgg gtttcgaacg attagtgagt attgtccaag gattacactc tgcctatgat accgacttat ttcttccatt aatgagaatt attcacgagt attcgaaagt tagaggatat ggtggacaac tgggggatat cgacacagca tatcgaattg tcgcggatca tcttcgtgct gcttgtatta tgatttcaga cggtgtcgaa cctagttcac gaaatcgagg gtatcatctt cgacgggttt tgcgacgtgc tgcattgaat tttaccctca cgcttggtgc agaacgcggg atgttagcat ctttagtgcc tgattttgtt aatcatataa ctttactgta caacaatgtc gcagcttgtg aaactgtaat agctaaaacg gttatgtctg aggaacagtt gttttggaga agttatgaca aaggctgtaa attacttgag cataatattg catctcagca acatgtgctg tcaggagaaa ttgcgtggat gttgtcagga acttacggtc ttccattgtc aataacccaa aagatatgta gagaaaaagg gttgaaagtt gatgtagatg gcttccaaca atgtctggct aattttcaaa aagctcaaaa agctgaagag gagctttggc aaaaaatcga tttagaagaa atgatactga atggtgtaga gccaacgaat gattcagaaa aatattgttg tgaacggatt gaattgggca agtatgaatt cccgagcagg acaggtactg ttgtggcaat ctttgatgtc aatggtaaaa atgtaatgtc gcttaatcca ggcgaattag ggtctgtcgt tatggattcg actatttttt ttgctgaaca aggagggcaa ttgtacgaca gaggcatcct tcaagataat ttaaataata ctatatttat tgtgaatagt gtgaaaagac gaaatggcta catcattcat acagggaaag tagcagataa tggaattttg gaaaaaggcg taaacttgac gcagattata gatcctaagc aacggttttt tttaatgtgt ggtcatactg ctacccatat tcttcatttt gctctcgaaa aagtatttgg tgtatcagtc cgtcagatgg gttcatttat tggccctgat aagttacatt ttgatttctt tattcctgac caaaaaatcg ctcttgaaaa gatcgaaaaa gtaatggcaa tggtgaataa tattattgaa agtaacataa acgttacggt gaaatacatt ttgcaagaag aattaggttc tctgcataag gatttttctg cattcgacat ttcacaagag gcatcctcca acggctcata tcggatcatt acagttgaga gttcggcatt actcggtcaa gatatcatcg aaccatgttg tgggacgcat gttttaaaca caagtgatat tggtcgattt gttataattg gtcagaaatc acgaggtgcc aatgtacgtc gaatttacgc agtaacaaat agcgctgctc tggaaagcat tcataatgcg acgaagctta aagatgaact atctgatgcg ttgagcaatg ttagcggaac cttcatcgat acgcagaggc tgttgaaaga ttttcaaaag aatcttccat tgccatccaa atcatcaata tatgaaatgc ttaaaatagc gaagaagaga aacaagtgcc taagtcagaa acttcgttta cctgatgcaa cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat