SSGCID: Target Page

BrmaA.18815.b

Serine protease Z688.6, putative

CENTER ID:	BrmaA.18815.b
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.18815.b.B2.GE44591	mature protein		20	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A4E9F7E6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFTKLKIIAI LLFIGNGNAL LLHDPVTHIK LPKQTKENKN CQWSEEWLEN VPIDHFSFHD NRVFRLRYLI STEHFVSNGP IFFYTGNEGN VELFAQNTGL MWDLAPEFNA AIIFAEHRFY GKSQPFGNES YATIRNLGYL SSEQALGDFA LLIYHLKNKR LLVAQNSSVI AFGGSYGGML AAWMRIKYPH LVEGSIASSA PVFWFIDMSS SVPQDAYNRI VKRSFLSSGC IEKNILDGWI ALKNLSLTTT GRAYLNGLFH LDKKSHLKIS TDWIMLKEYL EDIFESMAMV NYPYPTNYLA ELPGWPVKVA CQFFNSNKSK NDEELAQSMY GIMNLYYNYT GQKKTFCIKP DVCNDSAYGA LGDPFGWPWQ SCTEMVMQQC SSGPPNDFFI KNCPFSLKGQ ELYCINTFGK LGYTKALMRP HWSILNYGNR YPTATNIVFS NGYLDPWSAG GWSLKSRVMG SLISIIIKDG AHHYDLRGKH QLDTNSVKDA RRLEKFYIKY WLKEAKLKQQ KKNY

NT Sequence

atgtttacta aactgaaaat tattgcaata ttattattta ttggaaatgg aaatgcatta ctgctccacg atccagtaac tcatattaaa ttaccaaaac aaacaaagga aaataaaaat tgtcaatggt cagaagaatg gttggaaaat gttcctattg atcatttttc atttcatgat aatcgagttt tccgtttgcg ttatttaata agcacagaac actttgtttc taatggacca atttttttct atactggcaa tgaaggaaac gtcgaactgt ttgcgcaaaa tacaggtttg atgtgggatt tagcaccgga atttaatgca gccattatat tcgccgaaca tcgattttat gggaaatcac aaccgttcgg caacgaaagt tatgcaacaa tccggaatct tggttattta tcatctgaac aagcattagg tgatttcgcc cttctcatat accatcttaa aaataagcgt cttttggttg ctcaaaattc atctgtcata gcatttggtg ggtcgtatgg tggtatgctt gctgcatgga tgcgtatcaa atatccacat ttagtagaag ggtctatagc atcaagtgct ccggttttct ggtttattga tatgtcaagc tctgtgccgc aagatgcgta caatcgtatt gttaagcgat ctttccttag ttccggttgt atagagaaaa atatccttga tggatggatt gcacttaaaa atttgtcctt gactacaact ggtcgagctt atctaaatgg cttgtttcat ttggacaaaa agtcacactt gaaaataagc actgattgga taatgcttaa agaatatctc gaagatatat ttgaatccat ggcgatggtt aactatccat atccaactaa ctatctagct gaacttccag gatggcctgt taaagttgca tgtcaatttt tcaacagcaa taaatcaaaa aatgatgaag agttggcaca aagtatgtat ggaataatga atctctatta taattataca ggtcaaaaaa aaacattttg cattaaacca gatgtgtgca atgattccgc atatggagca cttggtgatc catttggttg gccatggcag tcttgtactg aaatggtaat gcagcaatgt tccagtggtc cacctaatga tttctttatt aagaattgcc catttagtct taaaggtcaa gaattatact gcatcaatac atttggaaaa cttggatata caaaagcttt aatgcggcca cattggagta ttttaaatta tggtaatcga tatccaaccg ccacgaatat tgtattcagt aacggttatt tggatccatg gtctgctggt ggttggtcat tgaaatcacg agtgatggga tcattgatta gcattatcat taaggatggc gcacatcatt acgatcttcg cggcaaacat caattggata cgaattcggt gaaagatgca agacgtttgg aaaaatttta tatcaaatat tggttgaaag aagcaaaatt gaaacaacag aaaaaaaatt attaa

Details for BrmaA.18815.b.B2.GE44591

HARVESTED ON:	1/17/2022
SEQUENCED ON:	1/20/2022
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHLL LHDPVTHIKL PKQTKENKNC QWSEEWLENV PIDHFSFHDN RVFRLRYLIS TEHFVSNGPI FFYTGNEGNV ELFAQNTGLM WDLAPEFNAA IIFAEHRFYG KSQPFGNESY ATIRNLGYLS SEQALGDFAL LIYHLKNKRL LVAQNSSVIA FGGSYGGMLA AWMRIKYPHL VEGSIASSAP VFWFIDMSSS VPQDAYNRIV KRSFLSSGCI EKNILDGWIA LKNLSLTTTG RAYLNGLFHL DKKSHLKIST DWIMLKEYLE DIFESMAMVN YPYPTNYLAE LPGWPVKVAC QFFNSNKSKN DEELAQSMYG IMNLYYNYTG QKKTFCIKPD VCNDSAYGAL GDPFGWPWQS CTEMVMQQCS SGPPNDFFIK NCPFSLKGQE LYCINTFGKL GYTKALMRPH WSILNYGNRY PTATNIVFSN GYLDPWSAGG WSLKSRVMGS LISIIIKDGA HHYDLRGKHQ LDTNSVKDAR RLEKFYIKYW LKEAKLKQQK KNY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttactgc tccacgatcc agtaactcat attaaattac caaaacaaac aaaggaaaat aaaaattgtc aatggtcaga agaatggttg gaaaatgttc ctattgatca tttttcattt catgataatc gagttttccg tttgcgttat ttaataagca cagaacactt tgtttctaat ggaccaattt ttttctatac tggcaatgaa ggaaacgtcg aactgtttgc gcaaaataca ggtttgatgt gggatttagc accggaattt aatgcagcca ttatattcgc cgaacatcga ttttatggga aatcacaacc gttcggcaac gaaagttatg caacaatccg gaatcttggt tatttatcat ctgaacaagc attaggtgat ttcgcccttc tcatatacca tcttaaaaat aagcgtcttt tggttgctca aaattcatct gtcatagcat ttggtgggtc gtatggtggt atgcttgctg catggatgcg tatcaaatat ccacatttag tagaagggtc tatagcatca agtgctccgg ttttctggtt tattgatatg tcaagctctg tgccgcaaga tgcgtacaat cgtattgtta agcgatcttt ccttagttcc ggttgtatag agaaaaatat ccttgatgga tggattgcac ttaaaaattt gtccttgact acaactggtc gagcttatct aaatggcttg tttcatttgg acaaaaagtc acacttgaaa ataagcactg attggataat gcttaaagaa tatctcgaag atatatttga atccatggcg atggttaact atccatatcc aactaactat ctagctgaac ttccaggatg gcctgttaaa gttgcatgtc aatttttcaa cagcaataaa tcaaaaaatg atgaagagtt ggcacaaagt atgtatggaa taatgaatct ctattataat tatacaggtc aaaaaaaaac attttgcatt aaaccagatg tgtgcaatga ttccgcatat ggagcacttg gtgatccatt tggttggcca tggcagtctt gtactgaaat ggtaatgcag caatgttcca gtggtccacc taatgatttc tttattaaga attgcccatt tagtcttaaa ggtcaagaat tatactgcat caatacattt ggaaaacttg gatatacaaa agctttaatg cggccacatt ggagtatttt aaattatggt aatcgatatc caaccgccac gaatattgta ttcagtaacg gttatttgga tccatggtct gctggtggtt ggtcattgaa atcacgagtg atgggatcat tgattagcat tatcattaag gatggcgcac atcattacga tcttcgcggc aaacatcaat tggatacgaa ttcggtgaaa gatgcaagac gtttggaaaa attttatatc aaatattggt tgaaagaagc aaaattgaaa caacagaaaa aaaattattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat