BrmaA.18809.b

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID: BrmaA.18809.b
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18809.b.B2.GE41805 XtalkPred domain 110 510
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0J9Y6Q0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAECLKWVGN FMIDEVWLSK KINDVIVDKD DQWRIKLFND IISEIGENFK YKSTPCKTVF GNGADTEDVN DQTITYNSDS NNELTVSENS KTNETTSALI RNLKLGKTTD NCASLNDKEI IQLLNFGHLK QRDLEQKLSY DFLRAVSLRR SHFYETANIS FQNLPFQQYD YSYVNGSCCE NVIGYVPVPT GIVGPLLVNG RKCFVPMSTT EGALVASTNR GCRAVFESGG VTVRMYRDGM TRAPVVQFAN VARTLEMKDF LDSSTGFEEI KAVFDSTSSF ARLQRLETDI TGRLLFIRFE AKTGDAMGMN MVSKGTNAAL SYLKQKCPEM EVLSLSGNYC VDKKASAINW IKGRGKSVVA EAVISAAVVQ TVLKTTVDAL VRLGQAKLLI GSSMAGTIGG WNAHAANIVA AIFIATGQDV AQVVSSSMCL TTMEKTELDD LYISCSMRCL EVGTVGGGTI LPAQNACLQM MGCVTATSVP GECAKHLAEI ICSTVLAGEL SLLAAQCSND LVKSHIRLNR STRCLMTSAD SVCDSRHESS SVTHDNDQQK KVRVEISFRD HPAEKPVKFE MQCSNIL
NT Sequence
atggcagaat gtctgaaatg ggtaggaaat ttcatgattg atgaagtatg gctttcgaaa aagataaatg acgttatcgt tgataaagat gaccagtgga gaatcaagtt attcaatgac atcatatcag aaataggcga aaattttaaa tacaaaagta ccccatgtaa aacagttttc gggaatggag cagatacgga agatgtgaat gatcagacga taacatacaa tagtgattca aataacgaat taacagtctc agaaaattcg aagactaatg aaacgacatc agcattaatc cgaaatttga agttaggtaa aacgacggat aattgcgcgt cattgaatga caaagaaata attcagctgt taaattttgg acatttaaaa cagcgagatt tagaacaaaa attaagttat gattttctcc gtgctgtttc attgcgaagg tcacattttt atgagacggc caatatatca ttccaaaatc taccattcca gcagtatgac tacagttacg tgaacggatc atgctgcgaa aatgttattg gttatgttcc ggtaccgacg ggaattgttg gcccattatt agtgaatggg cgtaaatgtt tcgtaccaat gtctacaacg gaaggtgctc ttgtcgctag taccaaccgt ggttgtcgtg cagtctttga atctggcggt gtaactgttc ggatgtatag ggatggaatg acgcgtgcgc cagtcgttca gtttgcaaat gtcgctagga cgctcgagat gaaggatttt ctagattcat caaccggttt cgaggaaatc aaagctgttt ttgattcaac aagcagtttt gcgagactac aaaggcttga gacagatatc actggtcgtc tgttatttat aagatttgaa gctaaaacag gtgatgcgat gggtatgaat atggtttcaa aaggtacaaa tgcagcatta tcatatctaa agcagaaatg tcccgaaatg gaggtattgt cgttatctgg aaattactgt gtagacaaaa aagctagcgc gattaattgg attaaaggac gtgggaaatc ggtggttgct gaggcagtaa tttcagctgc agtggtacaa actgtcctta aaactacggt tgatgcactg gtacgattag gacaggcaaa actattgatt ggttcctcca tggctggcac tattggtggt tggaatgcgc atgcagctaa cattgttgca gccatattta ttgcgactgg acaggatgtt gctcaggttg tatctagtag tatgtgttta actacaatgg aaaaaactga gcttgatgat ttgtatattt cctgtagcat gcgttgccta gaagttggaa ctgttggagg tggtacaata ttaccagctc aaaatgcatg tttgcagatg atgggttgcg taacagcaac ttcagtcccc ggtgaatgtg cgaaacattt ggcagaaatt atctgttcaa cagtgctagc gggtgaatta tcgcttttgg ctgcgcagtg ttcgaatgat ctcgttaaaa gtcacataag gcttaacagg tctaccagat gtttgatgac gtctgcggat tctgtatgtg attctcgaca tgaatcaagc tcagtaaccc atgacaacga tcaacagaag aaagtaaggg tagaaatctc atttcgggat catcccgcag agaagcccgt taaatttgaa atgcagtgct ccaatatttt gtga
Details for BrmaA.18809.b.B2.GE41805
HARVESTED ON: 8/7/2017
SEQUENCED ON: 8/12/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD NCASLNDKEI IQLLNFGHLK QRDLEQKLSY DFLRAVSLRR SHFYETANIS FQNLPFQQYD YSYVNGSCCE NVIGYVPVPT GIVGPLLVNG RKCFVPMSTT EGALVASTNR GCRAVFESGG VTVRMYRDGM TRAPVVQFAN VARTLEMKDF LDSSTGFEEI KAVFDSTSSF ARLQRLETDI TGRLLFIRFE AKTGDAMGMN MVSKGTNAAL SYLKQKCPEM EVLSLSGNYC VDKKASAINW IKGRGKSVVA EAVISAAVVQ TVLKTTVDAL VRLGQAKLLI GSSMAGTIGG WNAHAANIVA AIFIATGQDV AQVVSSSMCL TTMEKTELDD LYISCSMRCL EVGTVGGGTI LPAQNACLQM MGCVTATSVP GECAKHLAEI ICSTVLAGEL SXXAAQCSND
Validated NT Sequence
atcttactca cttaatcatt cgaacactgc gcagcnnnnn ncgataattc acccgctagc actgttgaac agataatttc tgccaaatgt ttcgcacatt caccggggac tgaagttgct gttacgcaac ccatcatctg caaacatgca ttttgagctg gtaatattgt accacctcca acagttccaa cttctaggca acgcatgcta caggaaatat acaaatcatc aagctcagtt ttttccattg tagttaaaca catactacta gatacaacct gagcaacatc ctgtccagtc gcaataaata tggctgcaac aatgttagct gcatgcgcat tccaaccacc aatagtgcca gccatggagg aaccaatcaa tagttttgcc tgtcctaatc gtaccagtgc atcaaccgta gttttaagga cagtttgtac cactgcagct gaaattactg cctcagcaac caccgatttc ccacgtcctt taatccaatt aatcgcgcta gcttttttgt ctacacagta atttccagat aacgacaata cctccatttc gggacatttc tgctttagat atgataatgc tgcatttgta ccttttgaaa ccatattcat acccatcgca tcacctgttt tagcttcaaa tcttataaat aacagacgac cagtgatatc tgtctcaagc ctttgtagtc tcgcaaaact gcttgttgaa tcaaaaacag ctttgatttc ctcgaaaccg gttgatgaat ctagaaaatc cttcatctcg agcgtcctag cgacatttgc aaactgaacg actggcgcac gcgtcattcc atccctatac atccgaacag ttacaccgcc agattcaaag actgcacgac aaccacggtt ggtactagcg acaagagcac cttccgttgt agacattggt acgaaacatt tacgcccatt cactaataat gggccaacaa ttcccgtcgg taccggaaca taaccaataa cattttcgca gcatgatccg ttcacgtaac tgtagtcata ctgctggaat ggtagatttt ggaatgatat attggccgtc tcataaaaat gtgaccttcg caatgaaaca gcacggagaa aatcataact taatttttgt tctaaatctc gctgttttaa atgtccaaaa tttaacagct gaattatttc tttgtcattc aatgacgcgc aattatccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHDN CASLNDKEII QLLNFGHLKQ RDLEQKLSYD FLRAVSLRRS HFYETANISF QNLPFQQYDY SYVNGSCCEN VIGYVPVPTG IVGPLLVNGR KCFVPMSTTE GALVASTNRG CRAVFESGGV TVRMYRDGMT RAPVVQFANV ARTLEMKDFL DSSTGFEEIK AVFDSTSSFA RLQRLETDIT GRLLFIRFEA KTGDAMGMNM VSKGTNAALS YLKQKCPEME VLSLSGNYCV DKKASAINWI KGRGKSVVAE AVISAAVVQT VLKTTVDALV RLGQAKLLIG SSMAGTIGGW NAHAANIVAA IFIATGQDVA QVVSSSMCLT TMEKTELDDL YISCSMRCLE VGTVGGGTIL PAQNACLQMM GCVTATSVPG ECAKHLAEII CSTVLAGELS LLAAQCSND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataatt gcgcgtcatt gaatgacaaa gaaataattc agctgttaaa ttttggacat ttaaaacagc gagatttaga acaaaaatta agttatgatt ttctccgtgc tgtttcattg cgaaggtcac atttttatga gacggccaat atatcattcc aaaatctacc attccagcag tatgactaca gttacgtgaa cggatcatgc tgcgaaaatg ttattggtta tgttccggta ccgacgggaa ttgttggccc attattagtg aatgggcgta aatgtttcgt accaatgtct acaacggaag gtgctcttgt cgctagtacc aaccgtggtt gtcgtgcagt ctttgaatct ggcggtgtaa ctgttcggat gtatagggat ggaatgacgc gtgcgccagt cgttcagttt gcaaatgtcg ctaggacgct cgagatgaag gattttctag attcatcaac cggtttcgag gaaatcaaag ctgtttttga ttcaacaagc agttttgcga gactacaaag gcttgagaca gatatcactg gtcgtctgtt atttataaga tttgaagcta aaacaggtga tgcgatgggt atgaatatgg tttcaaaagg tacaaatgca gcattatcat atctaaagca gaaatgtccc gaaatggagg tattgtcgtt atctggaaat tactgtgtag acaaaaaagc tagcgcgatt aattggatta aaggacgtgg gaaatcggtg gttgctgagg cagtaatttc agctgcagtg gtacaaactg tccttaaaac tacggttgat gcactggtac gattaggaca ggcaaaacta ttgattggtt cctccatggc tggcactatt ggtggttgga atgcgcatgc agctaacatt gttgcagcca tatttattgc gactggacag gatgttgctc aggttgtatc tagtagtatg tgtttaacta caatggaaaa aactgagctt gatgatttgt atatttcctg tagcatgcgt tgcctagaag ttggaactgt tggaggtggt acaatattac cagctcaaaa tgcatgtttg cagatgatgg gttgcgtaac agcaacttca gtccccggtg aatgtgcgaa acatttggca gaaattatct gttcaacagt gctagcgggt gaattatcgc ttttggctgc gcagtgttcg aatgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t