SSGCID: Target Page

BrmaA.18809.a

3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) (EC 1.1.1.34)

CENTER ID:	BrmaA.18809.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.18809.a.B2.GE41804	XtalPred domain		115	515

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0J9Y7P2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQGIRMAECL KWVGNFMIDE VWLSKKINDV IVDKDDQWRI KLFNDIISEI GENFKYKSTP CKTVFGNGAD TEDVNDQTIT YNSDSNNELT VSENSKTNET TSALIRNLKL GKTTDNCASL NDKEIIQLLN FGHLKQRDLE QKLSYDFLRA VSLRRSHFYE TANISFQNLP FQQYDYSYVN GSCCENVIGY VPVPTGIVGP LLVNGRKCFV PMSTTEGALV ASTNRGCRAV FESGGVTVRM YRDGMTRAPV VQFANVARTL EMKDFLDSST GFEEIKAVFD STSSFARLQR LETDITGRLL FIRFEAKTGD AMGMNMVSKG TNAALSYLKQ KCPEMEVLSL SGNYCVDKKA SAINWIKGRG KSVVAEAVIS AAVVQTVLKT TVDALVRLGQ AKLLIGSSMA GTIGGWNAHA ANIVAAIFIA TGQDVAQVVS SSMCLTTMEK TELDDLYISC SMRCLEVGTV GGGTILPAQN ACLQMMGCVT ATSVPGECAK HLAEIICSTV LAGELSLLAA QCSNDLVKSH IRLNRSTRCL MTSADSVCDS RHESSSVTHD NDQQKKVRVE ISFRDHPAEK PVKFEMQCSN IL

NT Sequence

atgcagggta taagaatggc agaatgtctg aaatgggtag gaaatttcat gattgatgaa gtatggcttt cgaaaaagat aaatgacgtt atcgttgata aagatgacca gtggagaatc aagttattca atgacatcat atcagaaata ggcgaaaatt ttaaatacaa aagtacccca tgtaaaacag ttttcgggaa tggagcagat acggaagatg tgaatgatca gacgataaca tacaatagtg attcaaataa cgaattaaca gtctcagaaa attcgaagac taatgaaacg acatcagcat taatccgaaa tttgaagtta ggtaaaacga cggataattg cgcgtcattg aatgacaaag aaataattca gctgttaaat tttggacatt taaaacagcg agatttagaa caaaaattaa gttatgattt tctccgtgct gtttcattgc gaaggtcaca tttttatgag acggccaata tatcattcca aaatctacca ttccagcagt atgactacag ttacgtgaac ggatcatgct gcgaaaatgt tattggttat gttccggtac cgacgggaat tgttggccca ttattagtga atgggcgtaa atgtttcgta ccaatgtcta caacggaagg tgctcttgtc gctagtacca accgtggttg tcgtgcagtc tttgaatctg gcggtgtaac tgttcggatg tatagggatg gaatgacgcg tgcgccagtc gttcagtttg caaatgtcgc taggacgctc gagatgaagg attttctaga ttcatcaacc ggtttcgagg aaatcaaagc tgtttttgat tcaacaagca gttttgcgag actacaaagg cttgagacag atatcactgg tcgtctgtta tttataagat ttgaagctaa aacaggtgat gcgatgggta tgaatatggt ttcaaaaggt acaaatgcag cattatcata tctaaagcag aaatgtcccg aaatggaggt attgtcgtta tctggaaatt actgtgtaga caaaaaagct agcgcgatta attggattaa aggacgtggg aaatcggtgg ttgctgaggc agtaatttca gctgcagtgg tacaaactgt ccttaaaact acggttgatg cactggtacg attaggacag gcaaaactat tgattggttc ctccatggct ggcactattg gtggttggaa tgcgcatgca gctaacattg ttgcagccat atttattgcg actggacagg atgttgctca ggttgtatct agtagtatgt gtttaactac aatggaaaaa actgagcttg atgatttgta tatttcctgt agcatgcgtt gcctagaagt tggaactgtt ggaggtggta caatattacc agctcaaaat gcatgtttgc agatgatggg ttgcgtaaca gcaacttcag tccccggtga atgtgcgaaa catttggcag aaattatctg ttcaacagtg ctagcgggtg aattatcgct tttggctgcg cagtgttcga atgatctcgt taaaagtcac ataaggctta acaggtctac cagatgtttg atgacgtctg cggattctgt atgtgattct cgacatgaat caagctcagt aacccatgac aacgatcaac agaagaaagt aagggtagaa atctcatttc gggatcatcc cgcagagaag cccgttaaat ttgaaatgca gtgctccaat attttgtga

Details for BrmaA.18809.a.B2.GE41804

HARVESTED ON:	8/7/2017
SEQUENCED ON:	9/20/2017
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDN CASLNDKEII QLLNFGHLKQ RDLEQKLSYD FLRAVSLRRS HFYETANISF QNLPFQQYDY SYVNGSCCEN VIGYVPVPTG IVGPLLVNGR KCFVPMSTTE GALVASTNRG CRAVFESGGV TVRMYRDGMT RAPVVQFANV ARTLEMKDFL DSSTGFEEIK AVFDSTSSFA RLQRLETDIT GRLLFIRFEA KTGDAMGMNM VSKGTNAALS YLKQKCPEME VLSLSGNYCV DKKASAINWI KGRGKSVVAE AVISAAVVQT VLKTTVDALV RLGQAKLLIG SSMAGTIGGW NAHAANIVAA IFIATGQDVA QVVSSSMCLT TMEKTELDDL YISCSMRCLE VGTVGGGTIL PAQNACLQMM GCVTATSVPG ECAKHLAEII CSTVLAGELS LLAAQCSND

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataatt gcgcgtcatt gaatgacaaa gaaataattc agctgttaaa ttttggacat ttaaaacagc gagatttaga acaaaaatta agttatgatt ttctccgtgc tgtttcattg cgaaggtcac atttttatga gacggccaat atatcattcc aaaatctacc attccagcag tatgactaca gttacgtgaa cggatcatgc tgcgaaaatg ttattggtta tgttccggta ccgacgggaa ttgttggccc attattagtg aatgggcgta aatgtttcgt accaatgtct acaacggaag gtgctcttgt cgctagtacc aaccgtggtt gtcgtgcagt ctttgaatct ggcggtgtaa ctgttcggat gtatagggat ggaatgacgc gtgcgccagt cgttcagttt gcaaatgtcg ctaggacgct cgagatgaag gattttctag attcatcaac cggtttcgag gaaatcaaag ctgtttttga ttcaacaagc agttttgcga gactacaaag gcttgagaca gatatcactg gtcgtctgtt atttataaga tttgaagcta aaacaggtga tgcgatgggt atgaatatgg tttcaaaagg tacaaatgca gcattatcat atctaaagca gaaatgtccc gaaatggagg tattgtcgtt atctggaaat tactgtgtag acaaaaaagc tagcgcgatt aattggatta aaggacgtgg gaaatcggtg gttgctgagg cagtaatttc agctgcagtg gtacaaactg tccttaaaac tacggttgat gcactggtac gattaggaca ggcaaaacta ttgattggtt cctccatggc tggcactatt ggtggttgga atgcgcatgc agctaacatt gttgcagcca tatttattgc gactggacag gatgttgctc aggttgtatc tagtagtatg tgtttaacta caatggaaaa aactgagctt gatgatttgt atatttcctg tagcatgcgt tgcctagaag ttggaactgt tggaggtggt acaatattac cagctcaaaa tgcatgtttg cagatgatgg gttgcgtaac agcaacttca gtccccggtg aatgtgcgaa acatttggca gaaattatct gttcaacagt gctagcgggt gaattatcgc ttttggctgc gcagtgttcg aatgattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t