BrmaA.18347.a

myosin heavy chain

CENTER ID: BrmaA.18347.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18347.a.B1.GE36421 truncation 1 612
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001891705.1
UniProt: A8NEA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
DQSKPYDSKK NVWIPDAEDG YIAAEIKSSK GDSVTVVSVR GNEVTLKAEI VQEMNPPKFE KTEDMSNLTF LNDASVLHNL RARYGQMLIY TYSGLFCVVI NPYKRLPIYE PSVAEMYMGK RRTEMPPHLF AVSDEAYRNM LIDHENQSML ITGESGAGKT ENTKKVIAYF ATVGASQQKG DAAAAATPDQ PDKKKVTLED QIVQTNPVLE AFGNAKTVRN NNSSRFGKFI RIHFSKLGRV ASCDIEHYLL EKSRVIRQAP GERCYHIFYQ LYSDFIPTLK KELLLDKPLK EYYFVAQAEL TIDGVNDKEE HQLTDEAFDI LHFSQEEKMN CYKLMAAIMH MGNMKFKQRP REEQAEPDGT DDAEKASAMY GIGHEEFLKA LTKPKVKVGN EWVNKGQNID QVTWAVGAMA KGLYSRVFNW LVKKCNKTLD QKGISRDFFI GVLDIAGFEI FDFNSFEQLW INFVNEKLQQ FFNHHMFVLE QEEYAREGIQ WTFIDFGLDL QACIELIEKP LGILSMLDEE CIVPKATDLT LAQKLNDQHL GKHPNFEKPK PPKGKQGEAH FAMRHYAGTV RYNVSNWLEK NKDPLNDTVV SVMKHSTGNL LLTEIWQDYT TQEEAAAAAK DGGGAGKKKG KSGSFMTVSM LYRESLNNLM TMLNMTHPHF IRCIIPNEKK QSGLLDAALV LNQLTCNGVL EGIRICRKGF PNRTLHKDFK QRYSILAANE SISSTDMRKC AEAMLSKIVK ENILTEENFR IGETKVFFKA GVLAHLEDVR DEALKTLLTK LQSQIKWYLG LTDKKRRIQQ KAGLLILQRN VRAWCSLRTW EWFKLYTKVR PMLKEGKIAE ELEKLQEKLK SLEETLQKEE NLRKNLDDSA KKMETEKAEL FEKLEAVKNN LTKSEGNLSQ MESAKSDADK KLEELNEQLA DVEDQNTEIQ RSKKKVEGEI DALKKQIQDL EMSARKAEME KQSKDHQIRS LQDELQQQAE SVAKLNKEIR HQEELNKKIT EDLQGEEDKT NHANKIKSKL EQTLNDLEDN LERERRAKAD SEKSKRKLEG ELKITQESID EATRQRRDLE NNMKRKEAEL NALSSRLEDE QATISKLQRQ IKELQSRAAE LEDEVQNERQ SRSKTDKARS DIQHELEELN DRLDEQGGAT AAQIEINKKR EAELAKLRRD IEEANMNHES QLAALRKKHT DAVAELTDQL DQIQKQKQKL EKDKMQYVRN TEDLAAQLDG ESAAKVSNEK LVKQFELQLN DLQARADEQS RQLHDYSSVR NRLGNENSDM IRQLEDAEEQ ISTLQRFKGQ LTQQIEDIRS ATDEEIRERQ VLAAQVKNLQ IEAEQAHNNL EEEIESKNEV ARQLSKANGE IQQWQSRFEQ DGMLQTTELE EVRRKQGIKI NELQEALEQA NQKIITLEKA RSRLLGDLDD AQVDVERANA TASQLEKKQK GFDALVDEWK KKADDMSAEL DSVQREVRNL STDLFKMKTT YEEYSDTAEG LRHENKALSN EIKDLTDQLG EGGRSQHEMQ KMIRRLEIEK EELQHALDEA EGALEAEESK VMRAQVEVSQ IRSEIEKRIQ EKEEDFENTR RNHQRALESM QASLEAESKS KNELLRVKKK LESDINELEI ALDHANKANA DAQKNLKRCT EQIREIQLQI EEEQRQRDEM KQHYYTAEKR AQILEAEKDE ISQALEQAER ARKQAELDAS ESRDQANELN MQVSNLGAAK RKAEGELQVN YSLPEEAEQA ALKGGKKVIA KLEQRIHDLE AELDGEQHRY QEANKNVAKS ERRVRELQFQ VDEDKKNYER LQDLVDKLQS KIKTQKKQLE DAEELANTNL QKYRQIQHQL EDAEERADNA ENSVTKMRSM SRTTMSVGPG TGLQATRSAT VLRSPSRNLL ERFCSTTNDS GECGDEAFPD VASLKPLGLQ QSTGEEELAN TNLQKYRQIQ HQLEDAEERA DNAENSVTKM RSMSRTTMSV GPGTGLQATR SATVLRSPSR NRISDY
NT Sequence
gatcagtcga aaccgtacga ttcaaagaaa aatgtttgga ttccggatgc ggaagatggt tatattgctg cggaaattaa atctagcaag ggtgactcag tcaccgtggt ttctgttcgt ggaaatgagg taacacttaa agcagaaatt gtgcaagaaa tgaatccacc aaaatttgaa aaaaccgaag atatgtcaaa tctgactttc ctgaatgatg cttccgttct gcataactta cgtgcacgtt acggtcaaat gctgatttac acatattcag gattgttctg tgttgtgata aatccgtata aaagacttcc aatttatgaa ccatccgtag ccgaaatgta catgggtaaa cgaagaaccg aaatgccacc acatttattt gctgtttctg atgaagctta tcgtaatatg ctgatcgatc atgaaaatca atcaatgtta attaccggtg aatccggtgc cggaaaaacc gaaaatacca aaaaagttat tgcctacttt gcaacggtag gtgcatctca acaaaaaggt gatgcagcag cagcggcaac accagatcaa ccggataaaa agaaggtaac tttggaggat caaattgttc aaacaaatcc ggtactggaa gcttttggta atgcaaaaac ggtccggaac aataattcat ctcgatttgg caaatttatt cgaattcact tctcgaaact aggtcgtgtc gcatcatgtg acattgaaca ctatttactt gaaaaatcac gtgttatacg tcaagcacca ggtgaacgat gctatcatat attttatcag ctgtattccg atttcatacc aacattaaag aaagaattat tattggataa gccattgaag gaatattatt ttgttgcaca agcagaacta acaattgatg gtgttaatga taaggaagag catcaattaa ctgatgaagc atttgatatt ttacattttt cacaagagga gaagatgaat tgttataaat taatggcagc aataatgcat atgggaaata tgaaatttaa acaacgacca cgtgaagagc aagcggaacc ggatggtaca gatgatgcgg aaaaagcatc agcaatgtac ggtattggtc atgaggaatt cttgaaagca ttaacaaagc caaaagttaa agttggcaat gaatgggtca ataaaggtca aaatattgat caagtaacat gggctgttgg agcaatggct aaaggtctat attcgagagt gtttaattgg cttgttaaaa aatgtaataa aacattggat caaaaaggta tatcaagaga tttcttcatt ggtgtattgg atattgctgg atttgaaata tttgatttca attcattcga acaactttgg attaattttg taaatgaaaa attacaacaa tttttcaatc atcatatgtt tgtattggag caagaagaat atgcccgaga aggcattcaa tggacattca ttgattttgg cttagattta caagcctgta ttgaattaat tgaaaaacca ttaggcattc tatcaatgtt agatgaggaa tgtatcgtac ctaaagcaac tgatcttaca ttggcgcaaa agttaaacga tcaacatttg ggtaaacatc caaattttga aaagccaaaa ccgccaaaag gaaaacaagg tgaagcgcat tttgctatgc gacattatgc tggtactgtt cgatacaatg tgagtaattg gttggaaaag aataaagatc cattaaatga tacggttgta tcagttatga aacattcaac tggtaattta cttctcacag aaatttggca agattatacg acacaggaag aagctgcagc tgctgctaaa gatggaggag gagcgggcaa aaagaagggt aaatccggtt cattcatgac cgtttctatg ttatatcgtg aatcattgaa taatctaatg acaatgttga atatgacaca tccgcatttt attcggtgta ttattccgaa tgaaaagaag caatccggtt tactggatgc ggcattagta ttaaatcagc ttacatgtaa tggtgtattg gaaggtattc gtatctgtcg taaaggtttc cctaacagaa cattacataa ggatttcaag caacgctact caatacttgc tgcaaatgaa tcgatcagta gcacagatat gagaaaatgt gctgaagcaa tgctaagtaa aattgttaaa gaaaatatat taacggaaga aaattttcga attggtgaaa caaaagtttt ctttaaagca ggtgtattag cacatttgga agatgttcgt gatgaagcat tgaaaacatt gctaacaaaa ttacaatcac aaattaaatg gtatcttggc ttaactgata aaaaacgacg tattcaacaa aaagctggct tattaatact acaacgtaat gttcgtgcgt ggtgttcatt acgtacgtgg gaatggttta aattatacac caaagtacga ccaatgttaa aagaaggtaa aatagccgaa gaattggaaa aattacagga gaaattaaaa tcacttgagg agacattaca aaaggaggaa aatttacgaa aaaatttgga tgatagtgca aaaaaaatgg aaaccgaaaa agcggaatta tttgaaaaat tagaagctgt aaaaaataat cttacaaaat ctgaaggtaa tctatcacaa atggaatcag caaaatcaga tgctgataaa aaacttgagg agttaaatga gcaacttgct gatgttgaag atcaaaatac agaaattcaa cgatcaaaaa agaaggttga aggtgaaatt gatgcattaa aaaagcaaat tcaagattta gaaatgagtg cacgtaaagc agaaatggaa aagcaatcaa aagatcatca aattcgttca cttcaagatg aattacaaca acaagcagaa tcggtggcaa aattaaacaa agaaattcga catcaggaag aattaaataa gaaaattacg gaagatttac aaggtgaaga agataaaaca aatcatgcaa ataagattaa aagtaaatta gagcaaacgt taaatgattt ggaggataat ttggaacgtg aacgacgtgc aaaagcagat agtgaaaaat caaagcgaaa acttgagggt gaattaaaaa ttacacaaga aagtattgat gaagcaacac gtcaacgacg tgacttagaa aataatatga aacgaaaaga agccgaatta aatgcattaa gtagtcgatt ggaagatgaa caagcaacaa ttagcaaatt gcaacgacaa attaaagaat tgcaaagtcg tgcagctgaa ttggaagatg aagtgcaaaa tgaacgacaa tcacgatcaa agactgataa ggcacgttca gatatacagc atgaattaga ggagttgaat gatcgactag atgagcaagg tggtgcaact gcagcacaaa ttgaaattaa taagaaacgt gaagctgaat tagctaaatt aagacgtgat attgaggagg caaatatgaa tcatgaaagt caactagcag cattacgtaa aaagcataca gatgctgtag ctgaactaac cgatcaatta gatcaaattc aaaaacaaaa gcaaaaattg gaaaaagata aaatgcaata tgtgcgtaat acggaagatt tagctgcaca attagatggt gaaagtgctg ctaaagtaag caatgaaaaa ttagtaaaac aatttgaatt acaattaaat gatttacaag cacgtgcaga tgagcaatca cgtcaattgc atgattatag ttcagtacgt aatcgtcttg gaaatgagaa tagtgatatg ataagacaat tagaagatgc agaagagcaa atttcaacat tacaacgatt taaaggacaa ttaacacaac aaattgagga tatacgttca gcaactgatg aagaaatacg tgaacggcaa gtgcttgcag cgcaagtgaa aaatttacaa attgaagcgg aacaagcaca taataattta gaggaagaaa ttgaatcaaa gaatgaagtt gcaagacaat taagtaaagc aaatggtgaa atacagcaat ggcaatcacg ttttgagcaa gatggtatgc ttcaaactac tgaacttgaa gaagtacgtc gtaaacaagg tattaaaatt aatgaattac aagaagcact tgaacaagct aatcaaaaaa ttataacatt ggaaaaagca cgatcacgtt tacttggtga tcttgatgat gcgcaagttg acgtagaacg agcaaatgct acagcaagtc agctagagaa gaagcagaaa ggttttgatg ctcttgttga tgaatggaaa aagaaagctg atgatatgtc agcagagctt gattctgttc aacgtgaagt acgtaatctg tcaacggatt tattcaaaat gaagacaaca tatgaggaat actctgatac agctgaagga cttcgtcatg aaaataaagc tttaagcaat gaaattaaag atttaactga tcagcttggt gaaggtggtc gttcacaaca tgaaatgcaa aaaatgattc gacgattgga aattgaaaaa gaagaattac aacatgcact tgatgaagca gaaggagcat tagaagcaga agaaagtaaa gtaatgcgtg cacaagtgga agtgtcacaa atacgttctg aaattgagaa gcgaatacaa gagaaggaag aagattttga aaatactcga cgaaatcatc aacgtgcatt agaatcgatg caagcatcac tagaagctga atcaaagagt aaaaatgaac ttttacgtgt aaagaagaag cttgaatcag atattaatga acttgaaatt gcacttgatc atgctaataa agcaaatgct gatgcacaaa agaatttgaa acgatgcaca gagcaaattc gtgaaattca attacagatc gaagaagaac aacgacaacg tgatgagatg aagcagcatt attatacagc ggaaaaacgt gcacaaattt tggaagctga aaaagatgaa attagccaag cgctagaaca agcggaacga gcacgtaaac aagcagaatt agacgcaagt gaaagccgtg atcaagctaa tgaattaaat atgcaagtta gcaatcttgg tgcagcaaag aggaaagctg aaggagaact acaggtaaat tattcattgc cagaagaagc agaacaagca gcactaaaag gtggtaaaaa agtgattgct aaattagaac aacgtattca tgatctcgaa gctgaacttg atggtgaaca acatcgttat caggaagcaa ataaaaatgt tgctaaatca gagaggcgag tacgtgaatt acaatttcaa gtggatgaag ataagaaaaa ttatgaacga ttgcaagatc ttgttgataa acttcaaagt aaaattaaaa cacaaaagaa gcaattggaa gatgctgaag agcttgcaaa tacaaatttg caaaaatatc gacaaattca gcatcaattg gaagatgctg aagagcgtgc tgataatgcg gaaaattccg tcacaaaaat gcgctcaatg agccggacaa caatgtcagt tggaccgggt accggtttgc aagcaactcg ttccgctacc gtactccgtt caccatctcg taatctcctt gaacgtttct gttcaacaac caatgatagc ggtgaatgtg gtgacgaagc ttttccggat gtagcatcac ttaaaccact tggactacaa caatctaccg gtgaagaaga gcttgcaaat acaaatttgc aaaaatatcg acaaattcag catcaattgg aagatgctga agagcgtgct gataatgcgg aaaattccgt cacaaaaatg cgctcaatga gccggacgac aatgtcagtt ggaccgggta ccggtttgca agcaactcgt tccgctaccg tactccgttc accatctcgt aatcgtattt ccgactatta g
Details for BrmaA.18347.a.B1.GE36421
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 73
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMT YSGLFCVVIN PYKRLPIYSP SIIKHYMGKR RNEMPPHLFA VSDEAYRNIL IDHENQSMLI TGESGAGKTE NTKKVITYFA IVGATQQKKD ESKKGGTLEE QIVQTNPVLE AFGNAKTVRN NNSSRFGKFI RTHFTKDGKL AGGDIEHYLF EKSRVVRQAP GERSYHIYYQ LMSGFHPKIR EELRLTNDIK YYHFCSQAEL TIDGVDDKEE MKITXEAFDI MGFEDHETHD LYSNVAGIMH MGEMKFKQRP XEEQAEPMER KXQRMLVFVL ALIMXNS
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgacat attcaggatt gttctgtgtg gtaatcaatc catacaaacg tttgcctatt tatagtccat ctatcatcaa acattacatg ggtaaaagac gaaacgaaat gccaccacat ttattcgctg tatccgatga agcatatcga aatattctca ttgatcatga gaatcaatca atgctgatta ccggtgagtc tggtgctggt aaaactgaaa acaccaaaaa ggtcattaca tactttgcta ttgtcggtgc cacacagcag aagaaagatg aaagcaagaa gggagggact ctagaagaac aaatcgtgca aactaatcca gtattggaag ctttcggtaa tgcaaaaacg gtccgaaata acaattcctc acgattcggc aaattcatcc gaactcattt cactaaagat gggaaactgg ctggtggtga tattgaacac tatctttttg aaaagtcacg tgttgtgcgc caagctcctg gtgaacgtag ttatcacatt tattatcaac ttatgtccgg ttttcatccg aaaataagag aagaactacg tttgactaac gatattaaat attatcactt ctgttcccaa gcagaactaa ccatcgatgg tgtagatgat aaagaagaaa tgaaaatcac gcangaagca tttgatatta tgggcttcga ggatcatgag actcatgacc tgtacagcaa tgtggctggc attatgcata tgggtgaaat gaaatttaag caacgaccac ntgaagagca agcagaaccg atggagagga agangcaaag aatgctggtt tttgttttgg cgttgattat gaanaattcc taaaagcttt aacaaagcnn cgtgttcgtg tcggcacaat ggnnataaag gccnnantnn anngngcatn ggntgnanan gcntngaanc tatcnnntcn anangtnaat gntntcgnnn nnngtannna atnaannnan naaatcnaaa annntttnnn cnnnnnnnnn nnnnnnnnnt nnnnnnnnnn ntnnnnnnnn nnannnnnnn nnnnnnnnnn nnannnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn nngnnnc
Expected Protein Sequence
MAHHHHHHMT YSGLFCVVIN PYKRLPIYEP SVAEMYMGKR RTEMPPHLFA VSDEAYRNML IDHENQSMLI TGESGAGKTE NTKKVIAYFA TVGASQQKGD AAAAATPDQP DKKKVTLEDQ IVQTNPVLEA FGNAKTVRNN NSSRFGKFIR IHFSKLGRVA SCDIEHYLLE KSRVIRQAPG ERCYHIFYQL YSDFIPTLKK ELLLDKPLKE YYFVAQAELT IDGVNDKEEH QLTDEAFDIL HFSQEEKMNC YKLMAAIMHM GNMKFKQRPR EEQAEPDGTD DAEKASAMYG IGHEEFLKAL TKPKVKVGNE WVNKGQNIDQ VTWAVGAMAK GLYSRVFNWL VKKCNKTLDQ KGISRDFFIG VLDIAGFEIF DFNSFEQLWI NFVNEKLQQF FNHHMFVLEQ EEYAREGIQW TFIDFGLDLQ ACIELIEKPL GILSMLDEEC IVPKATDLTL AQKLNDQHLG KHPNFEKPKP PKGKQGEAHF AMRHYAGTVR YNVSNWLEKN KDPLNDTVVS VMKHSTGNLL LTEIWQDYTT QEEAAAAAKD GGGAGKKKGK SGSFMTVSML YRESLNNLMT MLNMTHPHFI RCIIPNEKKQ SGLLDAALVL NQLTCNGVLE GIRICRKGFP N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacat attcaggatt gttctgtgtt gtgataaatc cgtataaaag acttccaatt tatgaaccat ccgtagccga aatgtacatg ggtaaacgaa gaaccgaaat gccaccacat ttatttgctg tttctgatga agcttatcgt aatatgctga tcgatcatga aaatcaatca atgttaatta ccggtgaatc cggtgccgga aaaaccgaaa ataccaaaaa agttattgcc tactttgcaa cggtaggtgc atctcaacaa aaaggtgatg cagcagcagc ggcaacacca gatcaaccgg ataaaaagaa ggtaactttg gaggatcaaa ttgttcaaac aaatccggta ctggaagctt ttggtaatgc aaaaacggtc cggaacaata attcatctcg atttggcaaa tttattcgaa ttcacttctc gaaactaggt cgtgtcgcat catgtgacat tgaacactat ttacttgaaa aatcacgtgt tatacgtcaa gcaccaggtg aacgatgcta tcatatattt tatcagctgt attccgattt cataccaaca ttaaagaaag aattattatt ggataagcca ttgaaggaat attattttgt tgcacaagca gaactaacaa ttgatggtgt taatgataag gaagagcatc aattaactga tgaagcattt gatattttac atttttcaca agaggagaag atgaattgtt ataaattaat ggcagcaata atgcatatgg gaaatatgaa atttaaacaa cgaccacgtg aagagcaagc ggaaccggat ggtacagatg atgcggaaaa agcatcagca atgtacggta ttggtcatga ggaattcttg aaagcattaa caaagccaaa agttaaagtt ggcaatgaat gggtcaataa aggtcaaaat attgatcaag taacatgggc tgttggagca atggctaaag gtctatattc gagagtgttt aattggcttg ttaaaaaatg taataaaaca ttggatcaaa aaggtatatc aagagatttc ttcattggtg tattggatat tgctggattt gaaatatttg atttcaattc attcgaacaa ctttggatta attttgtaaa tgaaaaatta caacaatttt tcaatcatca tatgtttgta ttggagcaag aagaatatgc ccgagaaggc attcaatgga cattcattga ttttggctta gatttacaag cctgtattga attaattgaa aaaccattag gcattctatc aatgttagat gaggaatgta tcgtacctaa agcaactgat cttacattgg cgcaaaagtt aaacgatcaa catttgggta aacatccaaa ttttgaaaag ccaaaaccgc caaaaggaaa acaaggtgaa gcgcattttg ctatgcgaca ttatgctggt actgttcgat acaatgtgag taattggttg gaaaagaata aagatccatt aaatgatacg gttgtatcag ttatgaaaca ttcaactggt aatttacttc tcacagaaat ttggcaagat tatacgacac aggaagaagc tgcagctgct gctaaagatg gaggaggagc gggcaaaaag aagggtaaat ccggttcatt catgaccgtt tctatgttat atcgtgaatc attgaataat ctaatgacaa tgttgaatat gacacatccg cattttattc ggtgtattat tccgaatgaa aagaagcaat ccggtttact ggatgcggca ttagtattaa atcagcttac atgtaatggt gtattggaag gtattcgtat ctgtcgtaaa ggtttcccta actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat