BrmaA.18316.a

glycogen synthase

CENTER ID: BrmaA.18316.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18316.a.B3.GE39486 folded domain 1 281
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001902152.1
UniProt: A8QFG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
TSTDANCKDV TVVAFIIYPA AANSFNVESL KGQAVCKQLH NTISRIKENL ASRMFEACLK GRIPDMEDLL LPDERIQLKR CILSAKRDNL PPICTHNMLD DACDPVLNAF RRTQLINQPF DRVKVIFHPE FLSSVSPLMN LDYEDFVRGC HMGVFPSYYE PWGYTPAECT VMGVPSVTTN LSGFGCFIQE QVQDPHTFGI FVIDRRFKEP NASIDELAKT LYDFTLLSRR QRIIMRNRTE RLSELIDWKT LGTFYREARR KALEVTHPNF MQVIEETVKK MSRPTSAPST PTTSRSVSPY DSDESDTAEQ EAFEAKAWAE AN
NT Sequence
acatcaactg atgcaaattg taaagatgtc accgtggttg catttattat ttatcccgca gcagcaaatt cattcaatgt ggaatcatta aagggacaag cggtatgcaa gcaattacat aatacaatat caagaataaa agaaaactta gcatcaagaa tgttcgaagc atgcttgaaa ggtcgtatac cagatatgga ggatttacta cttccagatg aacgtatcca attaaagcgt tgcatactaa gtgcaaagcg tgataatttg cctccaatat gtacgcataa tatgcttgat gatgcttgtg atcccgtatt aaatgctttc cgacgtactc aacttataaa tcaaccgttt gatcgcgtta aagtaatatt ccatccggaa tttctatcat ctgtttcacc cttaatgaat ttggattacg aagattttgt tcgcggttgt cacatgggtg tttttccaag ttactatgaa ccatggggtt atacaccagc tgaatgtacg gtaatgggtg ttccatcagt tacaaccaat ctatccggat ttggatgttt tattcaggag caagtgcagg atcctcatac attcgggatt tttgttattg atcgccgttt taaagaacct aatgcatcta ttgatgaact tgcaaaaact ctttatgatt ttacattact ttctcgtcgt cagcgtatta ttatgcgaaa tcgaacggaa agattaagcg aacttataga ttggaaaact cttggaacat tttatcgtga agcacgacga aaagcactgg aagttacgca tccaaatttc atgcaagtaa tcgaagaaac tgttaaaaaa atgtcacgac caacatcagc accatcaaca ccgactacaa gtcgatcggt gtcaccatac gatagcgatg aatcagacac cgcggaacaa gaagcatttg aagcaaaagc atgggcggaa gctaattag
Details for BrmaA.18316.a.B3.GE39486
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHMTXX DANCKDVTVV AFIIYPAAAN SFNVESLKGQ AVCKQLHNTI SRIKENLASR MFEACLKGRI PDMEDLLLPD ERIQLKRCIL SAKRDNLPPI CTHNMLDDAC DPVLNAFRRT QLINQPFDRV KVIFHPEFLS SVSPLMNLDY EDFVRGCHMG VFPSYYEPWG YTPAECTVMG VPSVTTNLSG FGCFIQEQVQ DPHTFGIFVI DRRFKEPNAS IDELAKTLYD FTLLSRRQRI IMRNRTERLS ELIDWKTLGT FYREARRKAL EVTHPNFMQV XXXTVKKM
Validated NT Sequence
ttactcactt acattttttt aacagtttnn nnnnttactt gcatgaaatt tggatgcgta acttccagtg cttttcgtcg tgcttcacga taaaatgttc caagagtttt ccaatctata agttcgctta atctttccgt tcgatttcgc ataataatac gctgacgacg agaaagtaat gtaaaatcat aaagagtttt tgcaagttca tcaatagatg cattaggttc tttaaaacgg cgatcaataa caaaaatccc gaatgtatga ggatcctgca cttgctcctg aataaaacaa ccaaatccgg atagattggt tgtaactgat ggaacaccca ttaccgtaca ttcagctggt gtataacccc atggttcata gtaacttgga aaaacaccca tgtgacaacc gcgaacaaaa tcttcgtaat ccaaattcat taagggtgaa acagatgata gaaattccgg atggaatatt actttaacgc gatcaaacgg ttgatttata agttgagtac gtcggaaagc atttaatacg ggatcacaag catcatcaag catattatgc gtacatattg gaggcaaatt atcacgcttt gcacttagta tgcaacgctt taattggata cgttcatctg gaagtagtaa atcctccata tctggtatac gacctttcaa gcatgcttcg aacattcttg atgctaagtt ttcttttatt cttgatattg tattatgtaa ttgcttgcat accgcttgtc cctttaatga ttccacattg aatgaatttg ctgctgcggg ataaataata aatgcaacca cggtgacatc tttacaattt gcatcagnnn atgtcatatg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHTS TDANCKDVTV VAFIIYPAAA NSFNVESLKG QAVCKQLHNT ISRIKENLAS RMFEACLKGR IPDMEDLLLP DERIQLKRCI LSAKRDNLPP ICTHNMLDDA CDPVLNAFRR TQLINQPFDR VKVIFHPEFL SSVSPLMNLD YEDFVRGCHM GVFPSYYEPW GYTPAECTVM GVPSVTTNLS GFGCFIQEQV QDPHTFGIFV IDRRFKEPNA SIDELAKTLY DFTLLSRRQR IIMRNRTERL SELIDWKTLG TFYREARRKA LEVTHPNFMQ VIEETVKKM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacatcaa ctgatgcaaa ttgtaaagat gtcaccgtgg ttgcatttat tatttatccc gcagcagcaa attcattcaa tgtggaatca ttaaagggac aagcggtatg caagcaatta cataatacaa tatcaagaat aaaagaaaac ttagcatcaa gaatgttcga agcatgcttg aaaggtcgta taccagatat ggaggattta ctacttccag atgaacgtat ccaattaaag cgttgcatac taagtgcaaa gcgtgataat ttgcctccaa tatgtacgca taatatgctt gatgatgctt gtgatcccgt attaaatgct ttccgacgta ctcaacttat aaatcaaccg tttgatcgcg ttaaagtaat attccatccg gaatttctat catctgtttc acccttaatg aatttggatt acgaagattt tgttcgcggt tgtcacatgg gtgtttttcc aagttactat gaaccatggg gttatacacc agctgaatgt acggtaatgg gtgttccatc agttacaacc aatctatccg gatttggatg ttttattcag gagcaagtgc aggatcctca tacattcggg atttttgtta ttgatcgccg ttttaaagaa cctaatgcat ctattgatga acttgcaaaa actctttatg attttacatt actttctcgt cgtcagcgta ttattatgcg aaatcgaacg gaaagattaa gcgaacttat agattggaaa actcttggaa cattttatcg tgaagcacga cgaaaagcac tggaagttac gcatccaaat ttcatgcaag taatcgaaga aactgttaaa aaaatgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t