BrmaA.18265.a

RNA-binding protein, putative

CENTER ID: BrmaA.18265.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.18265.a.B2.GE39621 ordered region 67 470
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128178
RefSeq: XP_001892111.1
UniProt: A8NID4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
NKIYSQMITK RNKKQNLEHF EYTKNTKEVK DEKTEILGSS KSGMKMVGEQ SIGQSSAFAV LDSQYKSWRL IVRNLPFKTT QEDLQTVFGN FGPLSEIVLP KCKDKRFPNS CAGFAFIQFR KRQNAMEAIE KLNMSEVLGR KIAVDYALSK DTYETVVHEE KQKNEEIKKE IKQEVESNDG SIIEKEGRDD SEIKEEVVSE GLSDEDSYKI EKKSEKQAAQ EFKEDKAVLE GRVVFIRNLS YETTDKELKE ALSKFGNISL AVLCYYAGSE HPKGTAFVHF ETADGAEKCL SAIDQAPGIL IGGRRIFGHQ ALPRSEAARI EKEKLSKKPK DKRNLFLLRA GFIRPGSTAA AGMSEADAEK RARMAVVARK KLKNLHMFVS PTRLVVHNLP KPLTDKAFRS MCFIAAGNPD AKITECRIWR DRNKLGTSGE AVSRGFGFVN FLNHEDALSA MKHLNNNPDI FTKEKRPIVE FSIENLVALR LRESRLKKSQ QNQKGNEQVI CDKNKLDLEQ TKKHLSAGGQ KPLPSYLGPK IRKRDANMKK AQVKQKIGKK RKLQVRMSGT VLKKRKTNHI AEATPKKKKT SRSKQLTKYL TLSG
NT Sequence
aataaaattt attcacagat gattacgaaa agaaacaaaa aacagaattt ggaacatttc gagtatacta aaaatactaa agaggtgaaa gatgaaaaga ctgaaatatt aggttcatcc aaatccggca tgaaaatggt tggagaacaa tctattggac agagtagtgc ttttgctgtg cttgattcgc agtacaaatc atggcgattg attgtgcgca atttaccatt taagacgaca caggaagatc tacaaacagt atttggcaac tttggcccac tcagtgaaat tgttttgccg aagtgcaaag ataaacggtt ccctaattcg tgtgctggtt tcgcgtttat ccaatttcgg aaacgtcaga atgccatgga agcaattgag aaattaaata tgtctgaggt actgggaaga aaaattgcag tggactatgc tttgtcgaaa gatacttacg aaacagtagt acatgaagaa aaacagaaga atgaagaaat taagaaagaa atcaagcaag aggttgagtc gaacgatggc agtatcattg agaaggaggg aagagacgat agcgaaatca aggaagaagt agtgagtgaa ggcttatctg atgaagatag ttacaaaatc gaaaagaaat cagaaaaaca agctgcacaa gaattcaagg aagacaaagc agtattggaa ggacgtgtcg tattcatcag aaacttgtca tatgaaacaa cagataagga gttgaaggaa gccttgtcca agtttgggaa catttcatta gcagttcttt gttattacgc gggtagtgag cacccaaagg gaaccgcttt tgtgcatttc gaaacagctg atggggcaga aaaatgctta tctgccattg atcaggctcc tggtatttta attggtggtc gtcgaatttt cggtcatcaa gcattgccaa gaagtgaagc agcaagaatt gaaaaggaga aactgtcaaa aaaaccaaaa gacaagcgaa atcttttcct tctacgtgct ggttttatac gacctggttc aactgctgca gctgggatga gtgaagcaga tgctgagaag cgtgcacgta tggcagtagt tgcacgaaaa aagctaaaga atcttcacat gtttgtctct ccaacgcgtt tggttgttca caatttacca aaacctttaa cagataaagc ctttagatcg atgtgcttta ttgcagctgg caatccagat gccaaaataa ctgaatgtcg gatttggcga gatagaaata aattaggtac gtctggtgaa gccgtatctc gaggttttgg ctttgtcaac tttttgaacc atgaggatgc attatcagct atgaagcatt taaataataa tccggatata tttaccaaag aaaagagacc cattgttgaa ttttctattg aaaacctggt tgctcttcga cttcgtgaat caaggctgaa gaagtcgcag cagaaccaaa agggtaatga acaagttata tgtgataaga ataaactgga tttggaacaa acaaagaagc atctttcggc tggtggacag aaacctcttc cttcttattt gggaccaaaa attcgaaaaa gagatgcaaa tatgaaaaaa gctcaggtga aacaaaaaat tggtaagaaa agaaaactgc aagtaagaat gtctggaaca gtattaaaga aaagaaagac aaatcatata gctgaagcga cgccgaagaa gaaaaagaca agtcgttcta aacagcttac aaaatatctt accctctcag gttga
Details for BrmaA.18265.a.B2.GE39621
HARVESTED ON: data unavailable
SEQUENCED ON: 10/23/2015
EXPECTED MW: 47kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS WRLIVRNLPF KTTQEDLQTV FGNFGPLSEI VLPKCKDKRF PNSCAGFAFI QFRKRQNAME AIEKLNMSEV LGRKIAVDYA LSKDTYETVV HEEKQKNEEI KKEIKQEVES NDGSIIEKEG RDDSEIKEEV VSEGLSDEDT YKIEKKSEKQ AAQEFKEDKA VLEGRVVFIR NLSYETTDKE LKEALSKFGN ISLAVLCYYA GSEHPKGTAF VHFETADGAE KCLSAIDQAP GILIGGRRIF GHQALPRSEA ARIEKEKLSK KPKDKRNLFL LRAGFIRPGS TAAAGMSEAD AEKRARMAVV ARKKLKNLHM FVSPTRLVVH NLPKPLTDKA FRSMCFIAAG NPDAKITECR IWRDRNKLGT SGEAVSRGFG FVNFLNHEDA LSAMKHLNNN PDIXX
Validated NT Sequence
ccatggctca ccaccaccac caccatatgt catggcgatt gattgtgcgc aatttaccat ttaagacgac acaggaagat ctacaaacag tatttggcaa ctttggccca ctcagtgaaa ttgttttgcc gaagtgcaaa gataaacggt tccctaattc gtgtgctggt ttcgcgttta tccaatttcg gaaacgtcag aatgccatgg aagcaattga gaaattaaat atgtctgagg tactgggaag aaaaattgca gtggactatg ctttgtcgaa agatacttac gaaacagtag tacatgaaga aaaacagaag aatgaagaaa ttaagaaaga aatcaagcaa gaggttgagt cgaacgatgg cagtatcatt gagaaggagg gaagagacga tagcgaaatc aaggaagaag tagtgagtga aggcttatct gatgaagata cttacaaaat cgaaaagaaa tcagaaaaac aagctgcaca agaattcaag gaagacaaag cagtattgga aggacgtgtc gtattcatca gaaacttgtc atatgaaaca acagataagg agttgaagga agccttgtcc aagtttggga acatttcatt agcagttctt tgttattacg cgggtagtga gcacccaaag ggaaccgctt ttgtgcattt cgaaacagct gatggggcag aaaaatgctt atctgccatt gatcaggctc ctggtatttt aattggtggt cgtcgaattt tcggtcatca agcattgcca agaagtgaag cagcaagaat tgaaaaggag aaactgtcaa aaaaaccaaa agacaagcga aatcttttcc ttctacgtgc tggttttata cgacctggtt caactgctgc agctgggatg agtgaagcag atgctgagaa gcgtgcacgt atggcagtag ttgcacgaaa aaagctaaag aaccttcaca tgtttgtctc tccaacgcgt ttggttgttc acaatttacc aaaaccttta acagataaag cctttagatc gatgtgcttt attgcagctg gcaatccaga tgccaaaata actgaatgtc ggatttggcg agatagaaat aaattaggta cgtctggtga agccgtatct cgaggttttg gctttgtcaa ctttttgaac catgaggatg cattatcagc tatgaagcat ttaaataata atccggatat annnnnna
Expected Protein Sequence
MAHHHHHHSW RLIVRNLPFK TTQEDLQTVF GNFGPLSEIV LPKCKDKRFP NSCAGFAFIQ FRKRQNAMEA IEKLNMSEVL GRKIAVDYAL SKDTYETVVH EEKQKNEEIK KEIKQEVESN DGSIIEKEGR DDSEIKEEVV SEGLSDEDSY KIEKKSEKQA AQEFKEDKAV LEGRVVFIRN LSYETTDKEL KEALSKFGNI SLAVLCYYAG SEHPKGTAFV HFETADGAEK CLSAIDQAPG ILIGGRRIFG HQALPRSEAA RIEKEKLSKK PKDKRNLFLL RAGFIRPGST AAAGMSEADA EKRARMAVVA RKKLKNLHMF VSPTRLVVHN LPKPLTDKAF RSMCFIAAGN PDAKITECRI WRDRNKLGTS GEAVSRGFGF VNFLNHEDAL SAMKHLNNNP DIFTKEKRPI VE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcatggc gattgattgt gcgcaattta ccatttaaga cgacacagga agatctacaa acagtatttg gcaactttgg cccactcagt gaaattgttt tgccgaagtg caaagataaa cggttcccta attcgtgtgc tggtttcgcg tttatccaat ttcggaaacg tcagaatgcc atggaagcaa ttgagaaatt aaatatgtct gaggtactgg gaagaaaaat tgcagtggac tatgctttgt cgaaagatac ttacgaaaca gtagtacatg aagaaaaaca gaagaatgaa gaaattaaga aagaaatcaa gcaagaggtt gagtcgaacg atggcagtat cattgagaag gagggaagag acgatagcga aatcaaggaa gaagtagtga gtgaaggctt atctgatgaa gatagttaca aaatcgaaaa gaaatcagaa aaacaagctg cacaagaatt caaggaagac aaagcagtat tggaaggacg tgtcgtattc atcagaaact tgtcatatga aacaacagat aaggagttga aggaagcctt gtccaagttt gggaacattt cattagcagt tctttgttat tacgcgggta gtgagcaccc aaagggaacc gcttttgtgc atttcgaaac agctgatggg gcagaaaaat gcttatctgc cattgatcag gctcctggta ttttaattgg tggtcgtcga attttcggtc atcaagcatt gccaagaagt gaagcagcaa gaattgaaaa ggagaaactg tcaaaaaaac caaaagacaa gcgaaatctt ttccttctac gtgctggttt tatacgacct ggttcaactg ctgcagctgg gatgagtgaa gcagatgctg agaagcgtgc acgtatggca gtagttgcac gaaaaaagct aaagaatctt cacatgtttg tctctccaac gcgtttggtt gttcacaatt taccaaaacc tttaacagat aaagccttta gatcgatgtg ctttattgca gctggcaatc cagatgccaa aataactgaa tgtcggattt ggcgagatag aaataaatta ggtacgtctg gtgaagccgt atctcgaggt tttggctttg tcaacttttt gaaccatgag gatgcattat cagctatgaa gcatttaaat aataatccgg atatatttac caaagaaaag agacccattg ttgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat