SSGCID: Target Page

BrmaA.18215.a

proteasome subunit alpha 1 (T01 family)

CENTER ID:	BrmaA.18215.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.18215.a.B1.GE36400	full length		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001895018.1
UniProt:	A8P6Q9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRNQYDSDV TVWSPQGRLH QVDYAVEAMK QGSATVGVKS NTHAVLVALK RAANELCSHQ KKIYELDTHA GVSIAGLLSD GRILARFLQT ECSSWRWDYK EPVPIQVLNN RIQLKLQANT QIYGRRPFGV GLVIAGYDER GTHIVKTDPS AEVVETLACS IGARSQSART YLERNLDQFP TANVEQLVEY ALLALRDTLP AEDSLSKKNT TIAIVGKGTP FKVMEDDDVQ PFLDRIAGVP RTGQQIGGEQ QGTAEPMQL

NT Sequence

atgtttcgta accagtacga cagcgatgtt acggtttgga gtcctcaggg tcgactccat caggttgact atgctgtgga agcaatgaaa caaggtagtg ctactgtcgg agtgaaaagc aatacgcatg ctgttctggt agcattgaag cgtgctgcaa atgaactatg ttcgcatcag aagaaaattt atgaactgga cacgcatgcc ggtgtttcca ttgcgggtct tctttctgat ggtcgcatac ttgcgcgttt tttgcaaacc gaatgttcgt catggagatg ggattacaaa gagccagtcc ctattcaggt tttaaacaat cgcattcaac taaaactaca ggcaaataca caaatttatg gaagacgtcc attcggtgtt ggacttgtta ttgctggtta tgatgaaaga ggaacacata tcgtaaagac tgatccatca gcagaagttg tcgaaacatt ggcgtgctca ataggcgctc gttctcaaag cgcacgtacg tatctggagc ggaatttaga tcaatttcct actgcaaatg ttgaacagtt ggttgagtat gccttgctag cactgagaga tacactaccc gctgaagata gtttatcaaa aaagaataca acaattgcta ttgtgggtaa aggtacaccg ttcaaagtaa tggaagatga cgatgtgcaa ccatttttag atcgcattgc aggtgttccg agaactggcc aacaaattgg cggtgaacaa caaggaacgg cagaacctat gcagttgtga

Details for BrmaA.18215.a.B1.GE36400

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF RNQYDSDVTV WSPQGRLHQV DYAVEAMKQG SATVGVKSNT HAVLVALKRA ANELCSHQKK IYELDTHAGV SIAGLLSDGR ILARFLQTEC SSWRWDYKEP VPIQVLNNRI QLKLQANTQI YGRRPFGVGL VIAGYDERGT HIVKTDPSAE VVETLACSIG ARSQSARTYL ERNLDQFPTA NVEQLVEYAL LALRDTLPAE DSLSKKNTTI AIVGKGTPFK VMEDDDVQPF LDRIAGVPRT GQQIGGEQQG TAEPMQL

Validated NT Sequence

tcttactcac ttacaactgc ataggttctg ccgttccttg ttgttcaccg ccaatttgtt ggccagttct cggaacacct gcaatgcgat ctaaaaatgg ttgcacatcg tcatcttcca ttactttgaa cggtgtacct ttacccacaa tagcaattgt tgtattcttt tttgataaac tatcttcagc gggtagtgta tctctcagtg ctagcaaggc atactcaacc aactgttcaa catttgcagt aggaaattga tctaaattcc gctccagata cgtacgtgcg ctttgagaac gagcgcctat tgagcacgcc aatgtttcga caacttctgc tgatggatca gtctttacga tatgtgttcc tctttcatca taaccagcaa taacaagtcc aacaccgaat ggacgtcttc cataaatttg tgtatttgcc tgtagtttta gttgaatgcg attgtttaaa acctgaatag ggactggctc tttgtaatcc catctccatg acgaacattc ggtttgcaaa aaacgcgcaa gtatgcgacc atcagaaaga agacccgcaa tggaaacacc ggcatgcgtg tccagttcat aaattttctt ctgatgcgaa catagttcat ttgcagcacg cttcaatgct accagaacag catgcgtatt gcttttcact ccgacagtag cactaccttg tttcattgct tccacagcat agtcaacctg atggagtcga ccctgaggac tccaaaccgt aacatcgctg tcgtactggt tacgaaacat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc gtaaccagta cgacagcgat gttacggttt ggagtcctca gggtcgactc catcaggttg actatgctgt ggaagcaatg aaacaaggta gtgctactgt cggagtgaaa agcaatacgc atgctgttct ggtagcattg aagcgtgctg caaatgaact atgttcgcat cagaagaaaa tttatgaact ggacacgcat gccggtgttt ccattgcggg tcttctttct gatggtcgca tacttgcgcg ttttttgcaa accgaatgtt cgtcatggag atgggattac aaagagccag tccctattca ggttttaaac aatcgcattc aactaaaact acaggcaaat acacaaattt atggaagacg tccattcggt gttggacttg ttattgctgg ttatgatgaa agaggaacac atatcgtaaa gactgatcca tcagcagaag ttgtcgaaac attggcgtgc tcaataggcg ctcgttctca aagcgcacgt acgtatctgg agcggaattt agatcaattt cctactgcaa atgttgaaca gttggttgag tatgccttgc tagcactgag agatacacta cccgctgaag atagtttatc aaaaaagaat acaacaattg ctattgtggg taaaggtaca ccgttcaaag taatggaaga tgacgatgtg caaccatttt tagatcgcat tgcaggtgtt ccgagaactg gccaacaaat tggcggtgaa caacaaggaa cggcagaacc tatgcagttg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat