BrmaA.17935.a

T-complex protein 1, beta subunit, putative

CENTER ID: BrmaA.17935.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17935.a.B1.GE36397 full length 1 529
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001897161.1
UniProt: A8NUR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPVQILKQN AHEERGEGAR LSSFVGAIAI GDLVKSTLGP KGMDKILLCG EGDSQQVQVT NDGATILKAI GVDNPAAKVL VEISMTQDSE VGDGTTSVTV LAAELLKEAE RLVAARIHPQ TIIAGYRRAL KIAQDALRAS AKTSSPTDMR DDLLKIARTT LGSKILAQHK EHFAQLAVDA VLRLKGSGCL ESIQIIKKLG GSMDDSYLDE GFLLEKRPGM YQPKKVENAK ILIANTPMDT DKVKVFGSRV RVDSVAKVAE LEVAEKQKMK DKVNKILAHK INVFINRQLI YNYPEQLFAD AGIMAIEHAD FEGIERLALV LGGEIVSTFD SPELVKLGSC DLIEEVMVGE DRLLRFSGVP IGEACSIVVR GSTQQILDEA ERSLHDALCV LVTHIKEALT VAGAGASEIL MSTAAMSESQ KIAGKESLAV EAFARALAQL PTYICDNAGL DSAEIVSKLR AYHSNNNHSM GIDIEKGEPT DVIEKGLIES FSVKLCMVSS AAEAAEQILR VDNIIKSAPR QRARDRRPC
NT Sequence
atgcttccag tacaaatatt aaaacaaaat gctcatgaag aacgaggtga aggagcacgt ttatcatcat ttgttggtgc aatcgcaatt ggtgatttag taaagtctac attgggaccg aaaggaatgg ataagatttt gttgtgtggt gaaggtgact cacagcaagt gcaggtaaca aatgatggag ctactatttt gaaagctatt ggtgttgaca atccagctgc gaaagttctc gtcgaaattt cgatgacaca agattcggaa gtgggtgatg gaacaacatc agtgaccgtt cttgctgctg agctattgaa agaagccgaa cgactggttg ctgcccgaat tcatccacag acaattattg ctggctatcg acgagcttta aaaatagccc aagatgcgct ccgagcatcg gctaaaactt ctagtccaac agatatgcgt gatgacttgt tgaaaatagc tcgaacaaca ctgggttcca agattttagc gcaacataag gaacattttg ctcaacttgc cgttgatgct gtgttgcgtc tgaaaggttc tggatgtctg gaatcaattc agattatcaa aaaactgggt ggttcgatgg atgacagtta tctggatgaa gggtttttgc ttgaaaaacg tccaggcatg taccaaccaa aaaaagttga aaatgcaaaa attctgattg ctaatacgcc gatggatacc gacaaggtga aagtattcgg ttcaagagtg cgtgttgatt ctgtagctaa agttgcagaa ctggaagttg ctgaaaaaca aaaaatgaag gacaaggtaa ataaaatcct agcacataaa attaacgtat ttatcaaccg tcagttgata tacaattatc cggagcagct ctttgctgat gctggtatca tggccatcga gcatgccgat tttgaaggaa tagaaagact cgctcttgtt cttggtggtg aaatcgtgtc aacatttgat tcacctgaat tagttaaact tggttcttgt gatctcatcg aggaagttat ggttggtgag gatcggctgc tgcgattttc tggtgttcct attggtgagg cttgcagtat tgttgtccgt ggttctactc aacaaattct tgatgaagcg gaaagatcac ttcatgacgc gttatgtgtc ttagtgactc atattaagga agcactcact gtcgccggcg caggagcgtc cgaaattttg atgagcactg cagcgatgtc cgaaagtcag aagattgctg ggaaggagtc gcttgctgta gaggcatttg ctcgtgcact ggcacaatta ccaacttaca tttgcgataa tgctggtctg gacagtgcag aaatcgtatc aaagttacgt gcttaccaca gcaataataa tcatagcatg ggaattgata ttgaaaaagg ggagccaacg gacgttattg aaaaaggtct gatcgagtcg ttcagtgtaa agttgtgtat ggtttccagt gcagcagaag cggctgagca gattcttcgt gtggacaata tcatcaaaag cgcaccacga caacgagcgc gcgatcgtag accttgttaa
Details for BrmaA.17935.a.B1.GE36397
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 90
Validated AA Sequence
MDKILLCGEG DSQQVQVTND GATILKAIGV DNPAAKVLVE ISMTQDSEVG DGTTSVTVLA AELLKEAERL VAARIHPQTI IAGYRRALKI AQDALRASAK TSSPTDMRDD LLKIARTTLG SKILAQHKEH FAQLAVDAVL RLKGSGCLES IQIIKKLGGS MDDSYLDEGF LLEKRPGMYX PKKVENAKIL IANTPMDTDK VKVFGSRVRV DSVAKVAELE VAEKQKMKDK VNKILAHKIN VFINRQLIYN YPEQLFADAG IMAIEHADFE GIERLALVLG GEIVSTFDSP ELVKLGSCDL IEEVMVGEDR LLRFSGVPIG EACSIVVRGS TQQILDEAER SLHDALCVLV THIKEALTVA GAGASEILMS TAAMSESQKI AGKESLAVEA FARALAQLPT YICDNAGLDS AEIVSKLRAY HSNNNHSMGI DIEKGEPTDV IEKGLIESFS VKLCMVSSAA EAAEQILRVD NIIKSAPRQR ARDRRPC
Validated NT Sequence
atcttactca cttaacaagg tctacgatcg cgcgctcgtt gtcgtggtgc gcttttgatg atattgtcca cacgaagaat ctgctcagcc gcttctgctg cactggaaac catacacaac tttacactga acgactcgat cagacctttt tcaataacgt ccgttggctc ccctttttca atatcaattc ccatgctatg attattattg ctgtggtaag cacgtaactt tgatacgatt tctgcactgt ccagaccagc attatcgcaa atgtaagttg gtaattgtgc cagtgcacga gcaaatgcct ctacagcaag cgactccttc ccagcaatct tctgactttc ggacatcgct gcagtgctca tcaaaatttc ggacgctcct gcgccggcga cagtgagtgc ttccttaata tgagtcacta agacacataa cgcgtcatga agtgatcttt ccgcttcatc aagaatttgt tgagtagaac cacggacaac aatactgcaa gcctcaccaa taggaacacc agaaaatcgc agcagccgat cctcaccaac cataacttcc tcgatgagat cacaagaacc aagtttaact aattcaggtg aatcaaatgt tgacacgatt tcaccaccaa gaacaagagc gagtctttct attccttcaa aatcggcatg ctcgatggcc atgataccag catcagcaaa gagctgctcc ggataattgt atatcaactg acggttgata aatacgttaa ttttatgtgc taggatttta tttaccttgt ccttcatttt ttgtttttca gcaacttcca gttctgcaac tttagctaca gaatcaacac gcactcttga accgaatact ttcaccttgt cggtatccat cggcgtatta gcaatcagaa tttttgcatt ttcaactttt tttggtnggt acatgcctgg acgtttttca agcaaaaacc cttcatccag ataactgtca tccatcgaac cacccagttt tttgataatc tgaattgatt ccagacatcc agaacctttc agacgcaaca cagcatcaac ggcaagttga gcaaaatgtt ccttatgttg cgctaaaatc ttggaaccca gtgttgttcg agctattttc aacaagtcat cacgcatatc tgttggacta gaagttttag ccgatgctcg gagcgcatct tgggctattt ttaaagctcg tcgatagcca gcaataattg tctgtggatg aattcgagca gcaaccagtc gttcggcttc tttcaatagc tcagcagcaa gaacggtcac tgatgttgtt ccatcaccca cttccgaatc ttgtgtcatc gaaatttcga cgagaacttt cgcagctgga ttgtcaacac caatagcttt caaaatagta gctccatcat ttgttacctg cacttgctgt gagtcacctt caccacacaa caaaatctta tccattcctt tcggtcccaa tgtagacttt actaaatcac caattgcgat tgcaccaaca aatgatgata aacgtgctcc ttcacctcgt tcttcatgag cattttgttt taatatttgt actggaa
Expected Protein Sequence
MAHHHHHHML PVQILKQNAH EERGEGARLS SFVGAIAIGD LVKSTLGPKG MDKILLCGEG DSQQVQVTND GATILKAIGV DNPAAKVLVE ISMTQDSEVG DGTTSVTVLA AELLKEAERL VAARIHPQTI IAGYRRALKI AQDALRASAK TSSPTDMRDD LLKIARTTLG SKILAQHKEH FAQLAVDAVL RLKGSGCLES IQIIKKLGGS MDDSYLDEGF LLEKRPGMYQ PKKVENAKIL IANTPMDTDK VKVFGSRVRV DSVAKVAELE VAEKQKMKDK VNKILAHKIN VFINRQLIYN YPEQLFADAG IMAIEHADFE GIERLALVLG GEIVSTFDSP ELVKLGSCDL IEEVMVGEDR LLRFSGVPIG EACSIVVRGS TQQILDEAER SLHDALCVLV THIKEALTVA GAGASEILMS TAAMSESQKI AGKESLAVEA FARALAQLPT YICDNAGLDS AEIVSKLRAY HSNNNHSMGI DIEKGEPTDV IEKGLIESFS VKLCMVSSAA EAAEQILRVD NIIKSAPRQR ARDRRPC
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcttc cagtacaaat attaaaacaa aatgctcatg aagaacgagg tgaaggagca cgtttatcat catttgttgg tgcaatcgca attggtgatt tagtaaagtc tacattggga ccgaaaggaa tggataagat tttgttgtgt ggtgaaggtg actcacagca agtgcaggta acaaatgatg gagctactat tttgaaagct attggtgttg acaatccagc tgcgaaagtt ctcgtcgaaa tttcgatgac acaagattcg gaagtgggtg atggaacaac atcagtgacc gttcttgctg ctgagctatt gaaagaagcc gaacgactgg ttgctgcccg aattcatcca cagacaatta ttgctggcta tcgacgagct ttaaaaatag cccaagatgc gctccgagca tcggctaaaa cttctagtcc aacagatatg cgtgatgact tgttgaaaat agctcgaaca acactgggtt ccaagatttt agcgcaacat aaggaacatt ttgctcaact tgccgttgat gctgtgttgc gtctgaaagg ttctggatgt ctggaatcaa ttcagattat caaaaaactg ggtggttcga tggatgacag ttatctggat gaagggtttt tgcttgaaaa acgtccaggc atgtaccaac caaaaaaagt tgaaaatgca aaaattctga ttgctaatac gccgatggat accgacaagg tgaaagtatt cggttcaaga gtgcgtgttg attctgtagc taaagttgca gaactggaag ttgctgaaaa acaaaaaatg aaggacaagg taaataaaat cctagcacat aaaattaacg tatttatcaa ccgtcagttg atatacaatt atccggagca gctctttgct gatgctggta tcatggccat cgagcatgcc gattttgaag gaatagaaag actcgctctt gttcttggtg gtgaaatcgt gtcaacattt gattcacctg aattagttaa acttggttct tgtgatctca tcgaggaagt tatggttggt gaggatcggc tgctgcgatt ttctggtgtt cctattggtg aggcttgcag tattgttgtc cgtggttcta ctcaacaaat tcttgatgaa gcggaaagat cacttcatga cgcgttatgt gtcttagtga ctcatattaa ggaagcactc actgtcgccg gcgcaggagc gtccgaaatt ttgatgagca ctgcagcgat gtccgaaagt cagaagattg ctgggaagga gtcgcttgct gtagaggcat ttgctcgtgc actggcacaa ttaccaactt acatttgcga taatgctggt ctggacagtg cagaaatcgt atcaaagtta cgtgcttacc acagcaataa taatcatagc atgggaattg atattgaaaa aggggagcca acggacgtta ttgaaaaagg tctgatcgag tcgttcagtg taaagttgtg tatggtttcc agtgcagcag aagcggctga gcagattctt cgtgtggaca atatcatcaa aagcgcacca cgacaacgag cgcgcgatcg tagaccttgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat