BrmaA.17369.c

vacuolar ATP synthase subunit B, putative

CENTER ID: BrmaA.17369.c
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17369.c.B1.GE36396 full length 1 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001893872.1
UniProt: A8NY32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKAAIDVNQG INGQKGVTIK NYKTNPRLTY KTVTGVNGPL VILGDVKFPQ YAEIVQLTLP DGTKRSGQVL EITRNKAVVQ VFEGTAGIDA KNTSCEFTGD ILRTPVSEDM LGRVLNGSGK PIDKGPPVLA EDFLDINGQP INPRCRIYPE EMIQTGISAI DVMNSIARGQ KIPIFSAAGL PHNEIAAQIV RQGGLVKLHD KHYDAKDDNF AIVFAAMGVN METARFFKQD FEENGSMENV CLFLNLANDP TIERIITPRL ALTVAEFLAY QCEKHVLVVL TDMSSYAEAL REVSAAREEV PGRRGFPGYM YTDLAAIYER AGRVEGRNGS ITQIPILSMP NDDITHPIPD LTGYITEGQI YVDRQLHNRQ IYPPINVLPS LSRLMKSAIG EGMTREDHSD VSNQLYACYA IGKDVQAMKA VVGEEALSSD DLLYLEFLTK FEKNFISQGN YENRTVFESL DIGWQLLRIF PREMLKRIPA STLDKYYPRG GGARDIKEMK GKEGK
NT Sequence
atgaaagcag ctattgatgt caatcaagga atcaatggtc aaaaaggtgt tactataaag aactataaga ccaatccacg tctcacatat aagacggtga ctggtgttaa tggtccattg gttatcctgg gtgatgtgaa atttcctcag tacgccgaaa ttgtacaatt gacactccct gatggaacga aacgttccgg acaggttctt gaaatcactc gcaataaagc tgttgttcag gtttttgaag gaactgcagg gatagatgcc aaaaatacat catgtgaatt caccggcgac attctgcgaa caccggtttc cgaagatatg cttggtcgtg tattaaatgg gtccggcaaa ccaattgata agggcccacc ggttttggca gaggatttcc tcgatatcaa cggtcagcca atcaatccaa gatgcaggat atatccagaa gagatgattc agacaggaat ttctgcaatc gatgtgatga attccattgc tcgcggtcag aaaataccta ttttttctgc tgccggtctt ccacataacg aaattgccgc acaaattgta cgccagggtg gacttgttaa attgcatgat aaacattatg atgcaaagga tgataacttt gctatcgttt ttgcggctat gggtgttaac atggaaaccg cacggttttt caagcaggat ttcgaagaga atggttccat ggagaatgtt tgcctgtttt tgaatcttgc taatgatcca accatcgaac gtattattac gccacgactt gcattgaccg ttgccgartt tttggcttat cagtgtgaga aacacgtact ggttgttctg actgatatgt cttcatatgc cgaagcgctt cgagaagtat cagcagctcg tgaagaagtg cctgggcggc gtggttttcc aggctacatg tatactgatt tggctgctat atatgaacgt gctggtcgtg tagaaggacg caatggctcg attactcaaa ttcctatctt gtcaatgccc aacgatgaca taacacatcc aatacctgac ttaacgggtt atattaccga aggacaaatc tacgtagacc gtcagttaca caaccgtcaa atctatccac cgatcaatgt actaccatcg ctgtcacgtc tgatgaaatc agccataggt gaaggtatga ctcgtgagga tcattcggat gtttccaatc aactatacgc atgttatgca atcggaaaag atgtgcaagc aatgaaagcc gttgtgggtg aagaagcttt gtcttcagat gatctacttt atctcgaatt tttgacgaag ttcgagaaga atttcatatc gcagggtaat tatgaaaatc gaacagtttt cgaatcctta gatatcggat ggcagttact gcgaatattt ccacgtgaaa tgttaaaacg tatccctgca tcaacattag acaagtatta tccaagagga ggaggtgcaa gggatatcaa agagatgaaa gggaaggaag gaaaataa
Details for BrmaA.17369.c.B1.GE36396
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMK AAIDVNQGIN GQKGVTIKNY KTNPRLTYKT VTGVNGPLVI LGDVKFPQYA EIVQLTLPDG TKRSGQVLEI TRNKAVVQVF EGTAGIDAKN TSCEFTGDIL RTPVSEDMLG RVLNGSGKPI DKGPPVLAED FLDINGQPIN PRCRIYPEEM IQTGISAIDV MNSIARGQKI PIFSAAGLPH NEIAAQIVRQ GGLVKLHDKH YDAKDDNFAI VFAAMGVNME TARXFKQDFX XNGSXENXCL FLNLANDPTI ERIITPDLH
Validated NT Sequence
tttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgaaagc agctattgat gtcaatcaag gaatcaatgg tcaaaaaggt gttactataa agaactataa gaccaatcca cgtctcacat ataagacggt gactggtgtt aatggtccat tggttatcct gggtgatgtg aaatttcctc agtacgccga aattgtacaa ttgacactcc ctgatggaac gaaacgttcc ggacaggttc ttgaaatcac tcgcaataaa gctgttgttc aggtttttga aggaactgca gggatagatg ccaaaaatac atcatgtgaa ttcaccggcg acattctgcg aacaccggtt tccgaagata tgcttggtcg tgtattaaat gggtccggca aaccaattga taagggccca ccggttttgg cagaggattt cctcgatatc aacggtcagc caatcaatcc aagatgcagg atatatccag aagagatgat tcagacagga atttctgcaa tcgatgtgat gaattccatt gctcgcggtc agaaaatacc tattttttct gctgccggtc ttccacataa cgaaattgcc gcacaaattg tacgccaggg tggacttgtt aaattgcatg ataaacatta tgatgcaaag gatgataact ttgctatcgt ttttgcggct atgggtgtta acatggaaac cgcacggntt ttcaagcagg atttcnaana gaatggntcc ntggagaatg nttgcctgtt tttgaatctt gctaatgatc caaccatcga acgtattatt acgcccgact tgcattgacc gttgccnant ttttggctta tcantgngag aaacacgtac tggntgttct gactganntg ncntcatatg ccgaancnnt ncnagaagta tcancagnnn nnngaagaan ngcctgggcg gcgngntttc nnngctacnt gnanactgat ttngnnnctn nnnntnangn gctggnngng nannannnnn cangnnngnt nnnncaantt nnnnnntgnc atgncnanna tganannnnn cnannnctga ctnnnggnnn nntncnnnan nnnnnnnnnn nnnnnncnnn nnnnnnnnnc nnnnnnnnnn nnnnccnnnn nnnnnnnnng nnnaannnnc ccnnngnnna
Expected Protein Sequence
MAHHHHHHMK AAIDVNQGIN GQKGVTIKNY KTNPRLTYKT VTGVNGPLVI LGDVKFPQYA EIVQLTLPDG TKRSGQVLEI TRNKAVVQVF EGTAGIDAKN TSCEFTGDIL RTPVSEDMLG RVLNGSGKPI DKGPPVLAED FLDINGQPIN PRCRIYPEEM IQTGISAIDV MNSIARGQKI PIFSAAGLPH NEIAAQIVRQ GGLVKLHDKH YDAKDDNFAI VFAAMGVNME TARFFKQDFE ENGSMENVCL FLNLANDPTI ERIITPRLAL TVAFLAYQCE KHVLVVLTDM SSYAEALREV SAAREEVPGR RGFPGYMYTD LAAIYERAGR VEGRNGSITQ IPILSMPNDD ITHPIPDLTG YITEGQIYVD RQLHNRQIYP PINVLPSLSR LMKSAIGEGM TREDHSDVSN QLYACYAIGK DVQAMKAVVG EEALSSDDLL YLEFLTKFEK NFISQGNYEN RTVFESLDIG WQLLRIFPRE MLKRIPASTL DKYYPRGGGA RDIKEMKGKE GK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cagctattga tgtcaatcaa ggaatcaatg gtcaaaaagg tgttactata aagaactata agaccaatcc acgtctcaca tataagacgg tgactggtgt taatggtcca ttggttatcc tgggtgatgt gaaatttcct cagtacgccg aaattgtaca attgacactc cctgatggaa cgaaacgttc cggacaggtt cttgaaatca ctcgcaataa agctgttgtt caggtttttg aaggaactgc agggatagat gccaaaaata catcatgtga attcaccggc gacattctgc gaacaccggt ttccgaagat atgcttggtc gtgtattaaa tgggtccggc aaaccaattg ataagggccc accggttttg gcagaggatt tcctcgatat caacggtcag ccaatcaatc caagatgcag gatatatcca gaagagatga ttcagacagg aatttctgca atcgatgtga tgaattccat tgctcgcggt cagaaaatac ctattttttc tgctgccggt cttccacata acgaaattgc cgcacaaatt gtacgccagg gtggacttgt taaattgcat gataaacatt atgatgcaaa ggatgataac tttgctatcg tttttgcggc tatgggtgtt aacatggaaa ccgcacggtt tttcaagcag gatttcgaag agaatggttc catggagaat gtttgcctgt ttttgaatct tgctaatgat ccaaccatcg aacgtattat tacgccacga cttgcattga ccgttgccga rtttttggct tatcagtgtg agaaacacgt actggttgtt ctgactgata tgtcttcata tgccgaagcg cttcgagaag tatcagcagc tcgtgaagaa gtgcctgggc ggcgtggttt tccaggctac atgtatactg atttggctgc tatatatgaa cgtgctggtc gtgtagaagg acgcaatggc tcgattactc aaattcctat cttgtcaatg cccaacgatg acataacaca tccaatacct gacttaacgg gttatattac cgaaggacaa atctacgtag accgtcagtt acacaaccgt caaatctatc caccgatcaa tgtactacca tcgctgtcac gtctgatgaa atcagccata ggtgaaggta tgactcgtga ggatcattcg gatgtttcca atcaactata cgcatgttat gcaatcggaa aagatgtgca agcaatgaaa gccgttgtgg gtgaagaagc tttgtcttca gatgatctac tttatctcga atttttgacg aagttcgaga agaatttcat atcgcagggt aattatgaaa atcgaacagt tttcgaatcc ttagatatcg gatggcagtt actgcgaata tttccacgtg aaatgttaaa acgtatccct gcatcaacat tagacaagta ttatccaaga ggaggaggtg caagggatat caaagagatg aaagggaagg aaggaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat