BrmaA.17369.b

ATP synthase beta chain, mitochondrial precursor, putative

CENTER ID: BrmaA.17369.b
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17369.b.B2.GE39618 ATPase 78 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17369.b.B2.PW37860 ATPase 78 548

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001900654.1
UniProt: A8Q895

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASRILTNFS RSVSRLLCDP KILRSVATRT YGSSVKILER TLIPVNCRKD LHSSTSVLAT ADAAKKVVSP PVKPTVKGSK GRIVAVIGAV VDVQFDEGLP PILNGLEVSG RKPRLILEVS QHLGDNVVRT IAMDGTEGLV RGDEVIDTGD PIKIPVGPET LGRIMNVIGE PIDERGPINS KHFSPIHAEA PEFVDMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELI NNVAKAHGGY SVFAGVGERT REGNDLYHEM IEGGVIDLKG KNSKVSLVYG QMNEPPGARA RVCLTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMG GMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRGIA ELAIYPAVDP LDSTSRIMDP NIVGQKHYDI ARGVQKILQD YKSLQDIIAI LGMDELSEED KLVVSRARKI QKFLSQPFQV AEVFTGHKGK FVTLEETIRG FELILKGEMD HLPEVAFYMQ GGIDDVVVKA EELAKQSG
NT Sequence
atggcttcac ggattctgac gaattttagc cggtctgttt cgcgtttgct gtgcgatcca aagatattaa ggagtgtggc tactcgaaca tatggttcgt cagttaaaat actagaaaga acactgattc cggttaactg ccgaaaggat ttacattcat ctacgtcagt attagctacg gcagatgctg ctaaaaaggt tgtatctcca ccggtgaagc cgactgtaaa aggttcgaag ggacgtatag tagcggtaat tggtgccgta gtggatgtgc aatttgatga aggtttaccg ccaattctga atggacttga agtatctggg cgtaaaccac gtcttatctt ggaagtttcg caacatctcg gcgacaatgt agtacgaaca atagctatgg acggtacaga aggacttgtt cgtggggatg aagtcataga tactggcgat ccgatcaaaa ttccagttgg accagaaact cttggtcgta tcatgaacgt tattggtgaa ccaattgatg agcgaggtcc tatcaattcc aaacattttt ctccaattca tgctgaagca ccagaatttg tcgatatgag cgtggaacaa gaaattcttg tgactggaat taaagttgtt gacttgctcg cgccgtatgc aaagggtggt aaaattggat tatttggcgg agccggtgtg ggaaaaactg tgttgattat ggaactgatc aataatgtcg ctaaagcgca cggtggttat tcagtatttg cgggtgttgg tgaacgtact cgagagggta atgatttgta tcacgaaatg attgaaggtg gagtcatcga tttgaaaggc aaaaattcga aagtatcact tgtatatggc caaatgaatg aaccgccggg agcacgagca cgggtttgtt taactggttt gacagttgct gaatattttc gtgatcaaga aggacaggat gtactgcttt tcattgacaa tattttccga tttacacaag ctggttccga agtatcagcg ctgcttgggc gcattccatc agctgtaggt tatcaaccaa ctttagcaac agatatgggt ggcatgcagg agcgaatcac cacaaccaaa aagggttcaa ttacatcagt acaagcgatt tatgtacctg ctgatgattt aacagatcca gctccagcaa ccacatttgc tcatttagat gctacaactg tattatcccg aggtattgct gaactggcta tttatcctgc cgtagacccg cttgattcaa catctcgcat catggatcct aatattgttg ggcagaaaca ttatgatatt gctcgcggtg tacaaaagat tttacaggat tacaagtcgt tgcaagatat tattgccatt cttggcatgg atgaattgtc agaagaagat aaattggtgg tgtcaagagc ccggaagatt cagaagtttt tgtctcagcc attccaggtt gcggaagttt tcaccggaca caaagggaaa tttgtaactc tcgaagaaac aatcagaggg tttgaactta ttttaaaagg tgagatggat catttgccag aagtggcatt ttatatgcaa ggtggaatcg atgatgtagt tgtaaaagct gaagaattag ctaagcagtc tggttga
Details for BrmaA.17369.b.B2.GE39618
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG SKGRIVAVIG AVVDVQFDEG LPPILNGLEV SGRKPRLILE VSQHLGDNVV RTIAMDGTEG LVRGDEVIDT GDPIKIPVGP ETLGRIMNVI GEPIDERGPI NSKHFSPIHA EAPEFVDMSV EQEILVTGIK VVDLLAPYAK GGKIGLFGGA GVGKTVLIME LINNVAKAHG GYSVFAGVGE RTREGNDLYH EMIEGGVIDL KGKNSKVSLV YGQMNEPPGA RARVCLTGLT VAEYFRDQEG QDVLLFIDNI FRFTQAGSEV SALLGRIPSA VGYQPTLATD MGGMQERITT TKKGSITSVQ AIYVPADDLT DPAPATTFAH LDATTVLSRG IAELAIYPAV DPLDSTSRIM DPNIVGQKHY DIARGVQKIL QDYKSLQDII AILGMDELSE EDKLVVSRAR KIQKFLSQPF QVAEVFTGHK GKFVTLEETI RGFELILKGE MDHLPEVAFY MQGGIDDVV
Validated NT Sequence
catggctcac caccaccacc accatatggg ttcgaaggga cgtatagtag cggtaattgg tgccgtagtg gatgtgcaat ttgatgaagg tttaccgcca attctgaatg gacttgaagt atctgggcgt aaaccacgtc ttatcttgga agtttcgcaa catctcggcg acaatgtagt acgaacaata gctatggacg gtacagaagg acttgttcgt ggggatgaag tcatagatac tggcgatccg atcaaaattc cagttggacc agaaactctt ggtcgtatca tgaacgttat tggtgaacca attgatgagc gaggtcctat caattccaaa catttttctc caattcatgc tgaagcacca gaatttgtcg atatgagcgt ggaacaagaa attcttgtga ctggaattaa agttgttgac ttgctcgcgc cgtatgcaaa gggtggtaaa attggattat ttggcggagc cggtgtggga aaaactgtgt tgattatgga actgatcaat aatgtcgcta aagcgcacgg tggttattca gtatttgcgg gtgttggtga acgtactcga gagggtaatg atttgtatca cgaaatgatt gaaggtggag tcatcgattt gaaaggcaaa aattcgaaag tatcacttgt atatggccaa atgaatgaac cgccgggagc acgagcacgg gtttgtttaa ctggtttgac agttgctgaa tattttcgtg atcaagaagg acaggatgta ctgcttttca ttgacaatat tttccgattt acacaagctg gttccgaagt atcagcgctg cttgggcgca ttccatcagc tgtaggttat caaccaactt tagcaacaga tatgggtggc atgcaggagc gaatcaccac aaccaaaaag ggttcaatta catcagtaca agcgatttat gtacctgctg atgatttaac agatccagct ccagcaacca catttgctca tttagatgct acaactgtat tatcccgagg tattgctgaa ctggctattt atcctgccgt agacccgctt gattcaacat ctcgcatcat ggatcctaat attgttgggc agaaacatta tgatattgct cgcggtgtac aaaagatttt acaggattac aagtcgttgc aagatattat tgccattctt ggcatggatg aattgtcaga agaagataaa ttggtggtgt caagagcccg gaagattcag aagtttttgt ctcagccatt ccaggttgcg gaagttttca ccggacacaa agggaaattt gtaactctcg aagaaacaat cagagggttt gaacttattt taaaaggtga gatggatcat ttgccagaag tggcatttta tatgcaaggt ggaatcgatg atgtagttgt a
Expected Protein Sequence
MAHHHHHHGS KGRIVAVIGA VVDVQFDEGL PPILNGLEVS GRKPRLILEV SQHLGDNVVR TIAMDGTEGL VRGDEVIDTG DPIKIPVGPE TLGRIMNVIG EPIDERGPIN SKHFSPIHAE APEFVDMSVE QEILVTGIKV VDLLAPYAKG GKIGLFGGAG VGKTVLIMEL INNVAKAHGG YSVFAGVGER TREGNDLYHE MIEGGVIDLK GKNSKVSLVY GQMNEPPGAR ARVCLTGLTV AEYFRDQEGQ DVLLFIDNIF RFTQAGSEVS ALLGRIPSAV GYQPTLATDM GGMQERITTT KKGSITSVQA IYVPADDLTD PAPATTFAHL DATTVLSRGI AELAIYPAVD PLDSTSRIMD PNIVGQKHYD IARGVQKILQ DYKSLQDIIA ILGMDELSEE DKLVVSRARK IQKFLSQPFQ VAEVFTGHKG KFVTLEETIR GFELILKGEM DHLPEVAFYM QGGIDDVVVK AEELAKQSG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggttcga agggacgtat agtagcggta attggtgccg tagtggatgt gcaatttgat gaaggtttac cgccaattct gaatggactt gaagtatctg ggcgtaaacc acgtcttatc ttggaagttt cgcaacatct cggcgacaat gtagtacgaa caatagctat ggacggtaca gaaggacttg ttcgtgggga tgaagtcata gatactggcg atccgatcaa aattccagtt ggaccagaaa ctcttggtcg tatcatgaac gttattggtg aaccaattga tgagcgaggt cctatcaatt ccaaacattt ttctccaatt catgctgaag caccagaatt tgtcgatatg agcgtggaac aagaaattct tgtgactgga attaaagttg ttgacttgct cgcgccgtat gcaaagggtg gtaaaattgg attatttggc ggagccggtg tgggaaaaac tgtgttgatt atggaactga tcaataatgt cgctaaagcg cacggtggtt attcagtatt tgcgggtgtt ggtgaacgta ctcgagaggg taatgatttg tatcacgaaa tgattgaagg tggagtcatc gatttgaaag gcaaaaattc gaaagtatca cttgtatatg gccaaatgaa tgaaccgccg ggagcacgag cacgggtttg tttaactggt ttgacagttg ctgaatattt tcgtgatcaa gaaggacagg atgtactgct tttcattgac aatattttcc gatttacaca agctggttcc gaagtatcag cgctgcttgg gcgcattcca tcagctgtag gttatcaacc aactttagca acagatatgg gtggcatgca ggagcgaatc accacaacca aaaagggttc aattacatca gtacaagcga tttatgtacc tgctgatgat ttaacagatc cagctccagc aaccacattt gctcatttag atgctacaac tgtattatcc cgaggtattg ctgaactggc tatttatcct gccgtagacc cgcttgattc aacatctcgc atcatggatc ctaatattgt tgggcagaaa cattatgata ttgctcgcgg tgtacaaaag attttacagg attacaagtc gttgcaagat attattgcca ttcttggcat ggatgaattg tcagaagaag ataaattggt ggtgtcaaga gcccggaaga ttcagaagtt tttgtctcag ccattccagg ttgcggaagt tttcaccgga cacaaaggga aatttgtaac tctcgaagaa acaatcagag ggtttgaact tattttaaaa ggtgagatgg atcatttgcc agaagtggca ttttatatgc aaggtggaat cgatgatgta gttgtaaaag ctgaagaatt agctaagcag tctggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BrmaA.17369.b.B2.PW37860
PURIFICATION DATe: 2/22/2016
CONCENTRATION: 28mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG SKGRIVAVIG AVVDVQFDEG LPPILNGLEV SGRKPRLILE VSQHLGDNVV RTIAMDGTEG LVRGDEVIDT GDPIKIPVGP ETLGRIMNVI GEPIDERGPI NSKHFSPIHA EAPEFVDMSV EQEILVTGIK VVDLLAPYAK GGKIGLFGGA GVGKTVLIME LINNVAKAHG GYSVFAGVGE RTREGNDLYH EMIEGGVIDL KGKNSKVSLV YGQMNEPPGA RARVCLTGLT VAEYFRDQEG QDVLLFIDNI FRFTQAGSEV SALLGRIPSA VGYQPTLATD MGGMQERITT TKKGSITSVQ AIYVPADDLT DPAPATTFAH LDATTVLSRG IAELAIYPAV DPLDSTSRIM DPNIVGQKHY DIARGVQKIL QDYKSLQDII AILGMDELSE EDKLVVSRAR KIQKFLSQPF QVAEVFTGHK GKFVTLEETI RGFELILKGE MDHLPEVAFY MQGGIDDVV
Validated NT Sequence
catggctcac caccaccacc accatatggg ttcgaaggga cgtatagtag cggtaattgg tgccgtagtg gatgtgcaat ttgatgaagg tttaccgcca attctgaatg gacttgaagt atctgggcgt aaaccacgtc ttatcttgga agtttcgcaa catctcggcg acaatgtagt acgaacaata gctatggacg gtacagaagg acttgttcgt ggggatgaag tcatagatac tggcgatccg atcaaaattc cagttggacc agaaactctt ggtcgtatca tgaacgttat tggtgaacca attgatgagc gaggtcctat caattccaaa catttttctc caattcatgc tgaagcacca gaatttgtcg atatgagcgt ggaacaagaa attcttgtga ctggaattaa agttgttgac ttgctcgcgc cgtatgcaaa gggtggtaaa attggattat ttggcggagc cggtgtggga aaaactgtgt tgattatgga actgatcaat aatgtcgcta aagcgcacgg tggttattca gtatttgcgg gtgttggtga acgtactcga gagggtaatg atttgtatca cgaaatgatt gaaggtggag tcatcgattt gaaaggcaaa aattcgaaag tatcacttgt atatggccaa atgaatgaac cgccgggagc acgagcacgg gtttgtttaa ctggtttgac agttgctgaa tattttcgtg atcaagaagg acaggatgta ctgcttttca ttgacaatat tttccgattt acacaagctg gttccgaagt atcagcgctg cttgggcgca ttccatcagc tgtaggttat caaccaactt tagcaacaga tatgggtggc atgcaggagc gaatcaccac aaccaaaaag ggttcaatta catcagtaca agcgatttat gtacctgctg atgatttaac agatccagct ccagcaacca catttgctca tttagatgct acaactgtat tatcccgagg tattgctgaa ctggctattt atcctgccgt agacccgctt gattcaacat ctcgcatcat ggatcctaat attgttgggc agaaacatta tgatattgct cgcggtgtac aaaagatttt acaggattac aagtcgttgc aagatattat tgccattctt ggcatggatg aattgtcaga agaagataaa ttggtggtgt caagagcccg gaagattcag aagtttttgt ctcagccatt ccaggttgcg gaagttttca ccggacacaa agggaaattt gtaactctcg aagaaacaat cagagggttt gaacttattt taaaaggtga gatggatcat ttgccagaag tggcatttta tatgcaaggt ggaatcgatg atgtagttgt a
Expressed Protein Sequence
MAHHHHHHGS KGRIVAVIGA VVDVQFDEGL PPILNGLEVS GRKPRLILEV SQHLGDNVVR TIAMDGTEGL VRGDEVIDTG DPIKIPVGPE TLGRIMNVIG EPIDERGPIN SKHFSPIHAE APEFVDMSVE QEILVTGIKV VDLLAPYAKG GKIGLFGGAG VGKTVLIMEL INNVAKAHGG YSVFAGVGER TREGNDLYHE MIEGGVIDLK GKNSKVSLVY GQMNEPPGAR ARVCLTGLTV AEYFRDQEGQ DVLLFIDNIF RFTQAGSEVS ALLGRIPSAV GYQPTLATDM GGMQERITTT KKGSITSVQA IYVPADDLTD PAPATTFAHL DATTVLSRGI AELAIYPAVD PLDSTSRIMD PNIVGQKHYD IARGVQKILQ DYKSLQDIIA ILGMDELSEE DKLVVSRARK IQKFLSQPFQ VAEVFTGHKG KFVTLEETIR GFELILKGEM DHLPEVAFYM QGGIDDVVVK AEELAKQSG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGTTCGA AGGGACGTAT AGTAGCGGTA ATTGGTGCCG TAGTGGATGT GCAATTTGAT GAAGGTTTAC CGCCAATTCT GAATGGACTT GAAGTATCTG GGCGTAAACC ACGTCTTATC TTGGAAGTTT CGCAACATCT CGGCGACAAT GTAGTACGAA CAATAGCTAT GGACGGTACA GAAGGACTTG TTCGTGGGGA TGAAGTCATA GATACTGGCG ATCCGATCAA AATTCCAGTT GGACCAGAAA CTCTTGGTCG TATCATGAAC GTTATTGGTG AACCAATTGA TGAGCGAGGT CCTATCAATT CCAAACATTT TTCTCCAATT CATGCTGAAG CACCAGAATT TGTCGATATG AGCGTGGAAC AAGAAATTCT TGTGACTGGA ATTAAAGTTG TTGACTTGCT CGCGCCGTAT GCAAAGGGTG GTAAAATTGG ATTATTTGGC GGAGCCGGTG TGGGAAAAAC TGTGTTGATT ATGGAACTGA TCAATAATGT CGCTAAAGCG CACGGTGGTT ATTCAGTATT TGCGGGTGTT GGTGAACGTA CTCGAGAGGG TAATGATTTG TATCACGAAA TGATTGAAGG TGGAGTCATC GATTTGAAAG GCAAAAATTC GAAAGTATCA CTTGTATATG GCCAAATGAA TGAACCGCCG GGAGCACGAG CACGGGTTTG TTTAACTGGT TTGACAGTTG CTGAATATTT TCGTGATCAA GAAGGACAGG ATGTACTGCT TTTCATTGAC AATATTTTCC GATTTACACA AGCTGGTTCC GAAGTATCAG CGCTGCTTGG GCGCATTCCA TCAGCTGTAG GTTATCAACC AACTTTAGCA ACAGATATGG GTGGCATGCA GGAGCGAATC ACCACAACCA AAAAGGGTTC AATTACATCA GTACAAGCGA TTTATGTACC TGCTGATGAT TTAACAGATC CAGCTCCAGC AACCACATTT GCTCATTTAG ATGCTACAAC TGTATTATCC CGAGGTATTG CTGAACTGGC TATTTATCCT GCCGTAGACC CGCTTGATTC AACATCTCGC ATCATGGATC CTAATATTGT TGGGCAGAAA CATTATGATA TTGCTCGCGG TGTACAAAAG ATTTTACAGG ATTACAAGTC GTTGCAAGAT ATTATTGCCA TTCTTGGCAT GGATGAATTG TCAGAAGAAG ATAAATTGGT GGTGTCAAGA GCCCGGAAGA TTCAGAAGTT TTTGTCTCAG CCATTCCAGG TTGCGGAAGT TTTCACCGGA CACAAAGGGA AATTTGTAAC TCTCGAAGAA ACAATCAGAG GGTTTGAACT TATTTTAAAA GGTGAGATGG ATCATTTGCC AGAAGTGGCA TTTTATATGC AAGGTGGAAT CGATGATGTA GTTGTAAAAG CTGAAGAATT AGCTAAGCAG TCTGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T