BrmaA.17369.a

vacuolar ATP synthase catalytic subunit A, osteoclast isoform, putative

CENTER ID: BrmaA.17369.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17369.a.B1.GE36394 full length 1 615
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001901660.1
UniProt: A8QEA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPTTKLMED AKESSYGFVY GVSGPVVTAE KMAGSAMYEL VRVGHSELVG EIIRLEGDYA TIQVYEDTSA VAIGDPVLRT GKPLSVELGP GIMGSIFDGI QRPLKDILEL TQSIYIPKGV NVNVLSRDAV WEFEPNRKLT VGDHITGGDT IGFVRENVLI NHRIMLPPIA CGTITYIAAA GEYRVTDVVL ETEFAGEKSK FTMLQIWPVR QPRPCAEKLA ANFPLLCGQR VLDAMFPCVQ GGTTAVPGAF GCGKTVISQS LSKFSNSDAI IYVGCGERGN EMSEVLRDFP ELTMEVNGVT TSIMKRTTLV ANTSNMPVAA REASIYTGIT LAEYFRDMGL NVSMMADSTS RWAEALREIS GRLGEMPADS GYPAYLAARL ASFYERAGKV KCLGSPEREG SVTIVGAVSP PGGDFADPVT SATLGIVQVF WGLDKKLAQR KHFPSINWLI SYSKYMRALD EYYDKNFPEF VALRTTCKEI LQEEEDLSEI VQLVGKASLA ESDKITLEVA KLIKDDFLQQ NGYTPYDRFC PFYKTVGMLK NMIAFYDLAK HAVESTAQAE NKITWAIIRD HMSDIMYELS SMKFKDPVKD GEQKIKKDYD ELLEQMQTAF RNLEE
NT Sequence
atgccaccga ctactaaatt gatggaagat gctaaagaga gctcctatgg ctttgtttac ggtgtttccg ggcctgtcgt aacagccgag aaaatggccg gttcagcaat gtacgagctg gtacgtgttg ggcattctga attggttggt gaaattatcc gtttggaagg tgattatgct acgattcaag tttatgaaga tacctcagcg gtagcaatag gtgatcctgt acttcggacc ggtaaaccat tgtcagtaga actaggaccc ggcattatgg gttccatttt cgatggtatt caacgaccct tgaaagatat tttagaacta acacaatcca tttatattcc caaaggtgtt aatgttaacg tgctttcacg tgatgcagtt tgggagtttg aaccaaatcg taaactcact gttggtgatc acattacggg tggtgataca atcggttttg tgcgtgaaaa cgtcctcatc aaccaccgaa taatgcttcc accaatagcg tgtggcacaa ttacgtatat agcagcagct ggtgaatatc gcgttactga tgttgtgttg gaaactgaat ttgctggtga aaaatccaaa ttcacaatgc ttcagatctg gcctgtgcgc cagccacgtc cgtgtgctga aaaattggca gctaactttc ccttactgtg tggtcaacgt gttctggatg caatgttccc ttgtgttcaa ggtggtacta ctgctgttcc aggagcattc ggctgtggta aaactgttat ttcacaatca ttatccaaat tctccaattc tgatgccatc atttatgttg gatgtggtga acgaggtaat gaaatgtcgg aagtactgcg tgactttcca gaactaacta tggaagtcaa cggtgtcact acatcaatta tgaagcgtac aactttggtc gctaacacat caaatatgcc tgtagctgca cgcgaagcat cgatttacac cggcattacg cttgctgaat attttcgtga catgggtcta aatgtttcaa tgatggctga ttctacatca cggtgggccg aagctcttcg tgaaatttct ggtcgtttgg gtgaaatgcc tgccgattct ggatacccgg catatttggc tgctcgtctg gcatcgttct atgaacgagc cggtaaagta aagtgcctcg gaagtccaga acgtgaagga tcggttacta ttgtgggtgc tgtttcacca cctggtggtg attttgctga tccagttacc tcggctactc ttggtattgt tcaagtgttc tggggattag acaagaaact ggcgcagcgc aaacactttc catccattaa ttggctaata tcatacagca aatatatgag agcactggat gaatattatg acaaaaattt cccagaattt gttgccctac gtacaacatg caaggaaatt cttcaagaag aagaggactt gtccgaaatt gtacaattag tgggtaaagc ttcattagct gagtcggaca aaattaccct agaagtagct aagttgatca aagacgactt tttgcaacaa aacggataca caccctatga ccgattctgt ccattctaca aaacggtggg aatgctgaaa aacatgattg ctttctatga tctagcaaaa catgctgtgg aatcaacagc acaagctgaa aataagatta cctgggcaat aattcgtgat cacatgagtg atattatgta cgagctatcg tctatgaaat tcaaagatcc agtcaaggat ggtgagcaaa aaattaaaaa agattatgat gaattattag aacaaatgca gactgcgttt agaaatttag aagaataa
Details for BrmaA.17369.a.B1.GE36394
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMP PTTKLMEDAK ESSYGFVYGV SGPVVTAEKM AGSAMYELVR VGHSELVGEI IRLEGDYATI QVYEDTSAVA IGDPVLRTGK PLSVELGPGI MGSIFDGIQR PLKDILELTQ SIYIPKGVNV NVLSRDAVWE FEPNRKLTVG DHITGGDTIG FVRENVLINH RIMLPPIACG TITYIAAAGE YRVTDVVLET EFAGEKSKFT MLQIWPVRQP RPCAEKLAAN FPLLCGQRVL DAMFPCVQGG TTAVPGAFGC GKTVISQSLS KFSNSDAXIY VGCGERGNEM SXYCVTXXXI XXXXVXTXXX RXXXSLXHXX CCSCXXXSXX XASXXXXXXX XXXXXXXDXX XGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttnttnnnnt taagaaggag anataccatg gctcaccacc accaccacca tatgccaccg actactaaat tgatggaaga tgctaaagag agctcctatg gctttgttta cggtgtttcc gggcctgtcg taacagccga gaaaatggcc ggttcagcaa tgtacgagct ggtacgtgtt gggcattctg aattggttgg tgaaattatc cgtttggaag gtgattatgc tacgattcaa gtttatgaag atacctcagc ggtagcaata ggtgatcctg tacttcggac cggtaaacca ttgtcagtag aactaggacc cggcattatg ggttccattt tcgatggtat tcaacgaccc ttgaaagata ttttagaact aacacaatcc atttatattc ccaaaggtgt taatgttaac gtgctttcac gtgatgcagt ttgggagttt gaaccaaatc gtaaactcac tgttggtgat cacattacgg gtggtgatac aatcggtttt gtgcgtgaaa acgtcctcat caaccaccga ataatgcttc caccaatagc gtgtggcaca attacgtata tagcagcagc tggtgaatat cgcgttactg atgttgtgtt ggaaactgaa tttgctggtg aaaaatccaa attcacaatg cttcagatct ggcctgtgcg ccagccacgt ccgtgtgctg aaaaattggc agctaacttt cccttactgt gtggtcaacg tgttctggat gcaatgttcc cttgtgttca aggtggtact actgctgttc cnggagcatt cggctgtggt aaaactgtta tttcacaatc attatccaaa ttctccaatt ctgatgccnt catttatgtt ggatgtggtg aacgaggtaa tgaaatgtcn nantactgcg tgactttncn nannatatng antnacgnng tcnctacnnc anntgancgt anantttngt cgctannnca tcnannatgc tgtagctgcn nncnancatc nanttannnn gcntcnntnn tgannnnttn nngnnnngnn nnnangnntn angnngnnga tnnnnnnnnn gggnnnnann nnnnnnnnnn nnnnnnngnn nnnnnnngnn nttnnnnnnn nngnnnnnnn ntnannnnnn nnnnannnnn cnnnnnnnnn nnnnnnnnnn ngg
Expected Protein Sequence
MAHHHHHHMP PTTKLMEDAK ESSYGFVYGV SGPVVTAEKM AGSAMYELVR VGHSELVGEI IRLEGDYATI QVYEDTSAVA IGDPVLRTGK PLSVELGPGI MGSIFDGIQR PLKDILELTQ SIYIPKGVNV NVLSRDAVWE FEPNRKLTVG DHITGGDTIG FVRENVLINH RIMLPPIACG TITYIAAAGE YRVTDVVLET EFAGEKSKFT MLQIWPVRQP RPCAEKLAAN FPLLCGQRVL DAMFPCVQGG TTAVPGAFGC GKTVISQSLS KFSNSDAIIY VGCGERGNEM SEVLRDFPEL TMEVNGVTTS IMKRTTLVAN TSNMPVAARE ASIYTGITLA EYFRDMGLNV SMMADSTSRW AEALREISGR LGEMPADSGY PAYLAARLAS FYERAGKVKC LGSPEREGSV TIVGAVSPPG GDFADPVTSA TLGIVQVFWG LDKKLAQRKH FPSINWLISY SKYMRALDEY YDKNFPEFVA LRTTCKEILQ EEEDLSEIVQ LVGKASLAES DKITLEVAKL IKDDFLQQNG YTPYDRFCPF YKTVGMLKNM IAFYDLAKHA VESTAQAENK ITWAIIRDHM SDIMYELSSM KFKDPVKDGE QKIKKDYDEL LEQMQTAFRN LEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccac cgactactaa attgatggaa gatgctaaag agagctccta tggctttgtt tacggtgttt ccgggcctgt cgtaacagcc gagaaaatgg ccggttcagc aatgtacgag ctggtacgtg ttgggcattc tgaattggtt ggtgaaatta tccgtttgga aggtgattat gctacgattc aagtttatga agatacctca gcggtagcaa taggtgatcc tgtacttcgg accggtaaac cattgtcagt agaactagga cccggcatta tgggttccat tttcgatggt attcaacgac ccttgaaaga tattttagaa ctaacacaat ccatttatat tcccaaaggt gttaatgtta acgtgctttc acgtgatgca gtttgggagt ttgaaccaaa tcgtaaactc actgttggtg atcacattac gggtggtgat acaatcggtt ttgtgcgtga aaacgtcctc atcaaccacc gaataatgct tccaccaata gcgtgtggca caattacgta tatagcagca gctggtgaat atcgcgttac tgatgttgtg ttggaaactg aatttgctgg tgaaaaatcc aaattcacaa tgcttcagat ctggcctgtg cgccagccac gtccgtgtgc tgaaaaattg gcagctaact ttcccttact gtgtggtcaa cgtgttctgg atgcaatgtt cccttgtgtt caaggtggta ctactgctgt tccaggagca ttcggctgtg gtaaaactgt tatttcacaa tcattatcca aattctccaa ttctgatgcc atcatttatg ttggatgtgg tgaacgaggt aatgaaatgt cggaagtact gcgtgacttt ccagaactaa ctatggaagt caacggtgtc actacatcaa ttatgaagcg tacaactttg gtcgctaaca catcaaatat gcctgtagct gcacgcgaag catcgattta caccggcatt acgcttgctg aatattttcg tgacatgggt ctaaatgttt caatgatggc tgattctaca tcacggtggg ccgaagctct tcgtgaaatt tctggtcgtt tgggtgaaat gcctgccgat tctggatacc cggcatattt ggctgctcgt ctggcatcgt tctatgaacg agccggtaaa gtaaagtgcc tcggaagtcc agaacgtgaa ggatcggtta ctattgtggg tgctgtttca ccacctggtg gtgattttgc tgatccagtt acctcggcta ctcttggtat tgttcaagtg ttctggggat tagacaagaa actggcgcag cgcaaacact ttccatccat taattggcta atatcataca gcaaatatat gagagcactg gatgaatatt atgacaaaaa tttcccagaa tttgttgccc tacgtacaac atgcaaggaa attcttcaag aagaagagga cttgtccgaa attgtacaat tagtgggtaa agcttcatta gctgagtcgg acaaaattac cctagaagta gctaagttga tcaaagacga ctttttgcaa caaaacggat acacacccta tgaccgattc tgtccattct acaaaacggt gggaatgctg aaaaacatga ttgctttcta tgatctagca aaacatgctg tggaatcaac agcacaagct gaaaataaga ttacctgggc aataattcgt gatcacatga gtgatattat gtacgagcta tcgtctatga aattcaaaga tccagtcaag gatggtgagc aaaaaattaa aaaagattat gatgaattat tagaacaaat gcagactgcg tttagaaatt tagaagaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat