SSGCID: Target Page

BrmaA.17143.c

tubulin alpha chain - mouse, putative

CENTER ID:	BrmaA.17143.c
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.17143.c.B1.GE36393	truncation		1	451

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001902537.1
UniProt:	A8QGK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFCQKREVIS LHIGQAGVQI GSACWELFCL EHGIDPSGRL NLQSDQLGKR DISSLTTFFS EAADGHYVPR TLLADLEPTV VDVVRTGKYK DLFHPLQMVT GREDAANNYA RGHYSIGKDM IDKVMDRIRR LAENCGGLQG FLLFHSFGGG TGSGFLSLLM EHLTIDYGKK SKLEFSIYPA PHISTSVVEP YNSVLMTHAT LEHADCSFIM DNEAIYDLYH HKLDVDRPSY PNLNRLIAQV VSSTTASLRF EGALNVDLIE FQTNLVPYPR IHFPLVTYAP IVSVKKACYE TLSVMDITRD CFETSNQMVK CDPTKGKYMS ICLLYRGNVT PNDVNEAISA VRHRRQINFV EWCPTGFKIG VNHEPPTAVP DGDTASVPRA LCMLANTTAI AEAWARLDAK FDLMYSKRAF THWFIGEGME EDEFIDARED MDLLERDYKE LAANEFDADS NDGNDF

NT Sequence

atgttttgtc aaaaacgtga agttatttcg ttgcatattg gacaagctgg agtacaaatt ggtagtgctt gttgggagct attttgcttg gagcatggta tcgatccgag cggtcgcctg aatttgcaat cggatcagtt ggggaaacgt gacatctcat cgttgacaac ttttttctcc gaagctgctg atgggcatta cgtgccccgt actttattgg ctgatttaga gccaacggtt gtcgatgtgg ttcgtacggg aaaatataag gatttatttc atccgctaca gatggtaact ggtcgtgagg atgcagcaaa taattatgcc cgtgggcatt attcaattgg taaagatatg attgataaag ttatggatcg tattcgacgt ttggctgaga attgtggtgg attgcaagga ttcctcctgt tccattcatt tggtggtggt accggttcag gttttttatc tctgctaatg gaacacttaa caattgatta tggtaaaaaa agcaagctag aattttcgat ctatccggca ccgcatattt cgacatctgt agtggagcca tataattctg tccttatgac tcatgctacc cttgagcatg ctgattgttc attcattatg gataatgagg caatttatga cttatatcat cataagttgg atgttgatcg accatcctat ccaaatctaa atcgtctcat tgctcaagtt gtttcgtcga ctaccgcttc gctgcgcttt gaaggtgcgc taaacgtaga tctgattgaa tttcagacaa atttggtgcc atatccacgt attcatttcc cattggtgac atatgctccg atagtttccg tcaagaaagc ttgttatgaa actctttcgg tgatggatat taccagagat tgttttgaaa cttccaatca aatggtgaaa tgtgatccga caaagggtaa atatatgtca atttgccttt tataccgtgg taatgtcaca ccaaatgatg tcaatgaagc cattagtgct gtccgtcatc gcagacagat taattttgta gagtggtgtc caactggatt caagattgga gttaaccatg agccaccaac agctgtacca gatggtgata ctgcatctgt accacgtgct ctctgcatgt tagccaatac aacagctatt gctgaagcat gggctcgttt ggatgccaaa ttcgatctga tgtattcaaa acgagctttt acacattggt ttattggtga gggtatggag gaagatgaat ttattgacgc acgtgaagac atggaccttc ttgagaggga ctataaggaa cttgcagcga atgaatttga tgctgattcg aacgatggca acgatttctg a

Details for BrmaA.17143.c.B1.GE36393

HARVESTED ON:	11/21/2012
SEQUENCED ON:	11/27/2012
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

annnnnnntt ntnnnnttta agaaggagan ataccatggc tcaccaccac caccaccata tgcgtgaagt tatttcgttg catattggac aagctggagt acaaattggt agtgcttgtt gggagctatt ttgcttggag catggtatcg atccgagcgg tcgcctgaat ttgcaatcgg atcagttggg gaaacgtgac atctcatcgt tgacaacttt tttctccgaa gctgctgatg ggcattacgt gccccgtact ttattggctg atttagagcc aacggttgtc gatgtggttc gtacgggaaa atataaggat ttatttcatc cgctacagat ggtaactggt cgtgaggatg cagcaaataa ttatgcccgt gggcattatt caattggtaa agatatgatt gataaagtta tggatcgtat tcgacgtttg gctgagaatt gtggtggatt gcaaggattc ctcctgttcc attcatttgg tggtggtacc ggttcaggtt ttttatctct gctaatggaa cacttaacaa ttgattatgg taaaaaaagc aagctagaat tttcgatcta tccggcaccg catatttcga catctgtagt ggagccatat aattctgtcc ttatgactca tgctaccctt gagcatgctg attgttcatt cattatggat aatgaggcaa tttatgactt atatcatcat aagttggatg ttgatcgacc atcctatcca aatctaaatc gtctcattgc tcaagttgtt tcgtcgacta ccgcttcgct gcgctttgaa gnngcgctaa acgtagatct gattgaattt canacaaatt tggtgccnta tccacgtatt catttcccat tggtgacata tgctcnatag tttccgtcan naaagctngn tatgaanctc tttcggtgat ggatnntacc agagatngtt tgaaacttnn ntcnannggt gaaannnnan cnanaanggn aannatannt canttgcntt nnaccnnngn angtcnnnnc natgatgnca nnannntann gctnncntca tcnnnnnnnn nnnnttngnn nnnnnnnngn ntnnnannnn nnnntnnnnn nnnannncnn nncnnangnn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnaann nnnnnnnnnn nttnnnnnnn nnnnt

Expected Protein Sequence

MAHHHHHHMR EVISLHIGQA GVQIGSACWE LFCLEHGIDP SGRLNLQSDQ LGKRDISSLT TFFSEAADGH YVPRTLLADL EPTVVDVVRT GKYKDLFHPL QMVTGREDAA NNYARGHYSI GKDMIDKVMD RIRRLAENCG GLQGFLLFHS FGGGTGSGFL SLLMEHLTID YGKKSKLEFS IYPAPHISTS VVEPYNSVLM THATLEHADC SFIMDNEAIY DLYHHKLDVD RPSYPNLNRL IAQVVSSTTA SLRFEGALNV DLIEFQTNLV PYPRIHFPLV TYAPIVSVKK ACYETLSVMD ITRDCFETSN QMVKCDPTKG KYMSICLLYR GNVTPNDVNE AISAVRHRRQ INFVEWCPTG FKIGVNHEPP TAVPDGDTAS VPRALCMLAN TTAIAEAWAR LDAKFDLMYS KRAFTHWFIG EGMEEDEFID AREDMDLLER DYKELAANEF DADSNDGNDF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg aagttatttc gttgcatatt ggacaagctg gagtacaaat tggtagtgct tgttgggagc tattttgctt ggagcatggt atcgatccga gcggtcgcct gaatttgcaa tcggatcagt tggggaaacg tgacatctca tcgttgacaa cttttttctc cgaagctgct gatgggcatt acgtgccccg tactttattg gctgatttag agccaacggt tgtcgatgtg gttcgtacgg gaaaatataa ggatttattt catccgctac agatggtaac tggtcgtgag gatgcagcaa ataattatgc ccgtgggcat tattcaattg gtaaagatat gattgataaa gttatggatc gtattcgacg tttggctgag aattgtggtg gattgcaagg attcctcctg ttccattcat ttggtggtgg taccggttca ggttttttat ctctgctaat ggaacactta acaattgatt atggtaaaaa aagcaagcta gaattttcga tctatccggc accgcatatt tcgacatctg tagtggagcc atataattct gtccttatga ctcatgctac ccttgagcat gctgattgtt cattcattat ggataatgag gcaatttatg acttatatca tcataagttg gatgttgatc gaccatccta tccaaatcta aatcgtctca ttgctcaagt tgtttcgtcg actaccgctt cgctgcgctt tgaaggtgcg ctaaacgtag atctgattga atttcagaca aatttggtgc catatccacg tattcatttc ccattggtga catatgctcc gatagtttcc gtcaagaaag cttgttatga aactctttcg gtgatggata ttaccagaga ttgttttgaa acttccaatc aaatggtgaa atgtgatccg acaaagggta aatatatgtc aatttgcctt ttataccgtg gtaatgtcac accaaatgat gtcaatgaag ccattagtgc tgtccgtcat cgcagacaga ttaattttgt agagtggtgt ccaactggat tcaagattgg agttaaccat gagccaccaa cagctgtacc agatggtgat actgcatctg taccacgtgc tctctgcatg ttagccaata caacagctat tgctgaagca tgggctcgtt tggatgccaa attcgatctg atgtattcaa aacgagcttt tacacattgg tttattggtg agggtatgga ggaagatgaa tttattgacg cacgtgaaga catggacctt cttgagaggg actataagga acttgcagcg aatgaatttg atgctgattc gaacgatggc aacgatttct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat