BrmaA.17143.b

tubulin alpha chain - mouse, putative

CENTER ID: BrmaA.17143.b
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17143.b.B2.GE39617 full length 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001896467.1
UniProt: A8PGR6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MREVISVHVG QAGVQIGNAA WELFCLEHGI QPDGTTCKIQ NKQHADNSFS TFFLETGTGR HVPRAILIDL EPSVIGPYKR LFHPEQMVTG KEDAANNYAR GHYTVGRELI DVVLDRIRRL AENCTGLQGF LIFHSFGGGT GSGFTSLLME RLTTDYDKKS KLEFSIYPAP QVSTAVVEPY NSILTTHTTL EHSDCSFMVD NEAIYDICRR NLDVERPSYL NLNRLISQVV SSITASLRFD GALNVDLTEF QTNLVPYPRI HFPLATYAPI ISAEKAYHES LSVMDITNSC FEPANQMVKC DPRLGKYMAV CLLYRGDVTS KDVNAAINSI KTKRTIQFVD WCPTGFKIGI NYQPPTVVPG GDVAKAPRAV SMLANTTAIA EAWARLDYKF DLMYAKRAFV HWYVGEGMEE GEFTEAREDL AALERDYDEV GFDSNDDNDG EDAMY
NT Sequence
atgcgtgaag taatctcagt tcatgttgga caagctggtg tacaaattgg taatgctgcg tgggaattat tttgtttgga acatggaata caaccggatg gaacaacatg caaaatccag aataaacaac atgctgataa ttcattttca acattcttcc tagaaaccgg taccggacga catgtaccac gagccatttt aattgatctt gaaccatctg ttatcggacc atataaacgt ttatttcatc ctgaacaaat ggttaccggt aaagaagatg cagcaaataa ttatgcacgt ggacattata cagttggtag agaattaatt gatgttgttc tggatcgaat aagacgactt gccgaaaatt gtaccggttt acagggtttt cttatatttc attcatttgg tggtggtaca ggttccggtt ttacatcatt attaatggaa cgattaacaa ctgattatga taaaaaaagt aaattggaat tttcaatcta tccagcacca caagtatcta cagctgttgt cgaaccatat aattcaattt taaccacaca tacaacttta gagcattctg attgctcatt tatggttgat aatgaagcaa tttatgatat ctgtcgtcgt aatttagatg tagaacgacc atcatatctc aatcttaatc gtttaatttc acaggttgtt tcatcaatta cagcttcatt acgttttgat ggtgcattaa atgtagattt gacagaattt caaacaaatt tagtaccata tccacgtata cattttccat tagctactta tgctccaata atttctgctg aaaaagctta tcatgaatcg ttatctgtaa tggatatcac taattcatgc tttgaaccag ctaatcaaat ggttaaatgt gatccacgtc ttggaaaata catggcagtt tgtcttttat atcgtggtga tgtgacatct aaagatgtta atgcagcaat taatagtatt aaaacaaaac gtacaataca atttgttgat tggtgtccaa caggtttcaa aataggtatc aattatcaac caccaacagt tgtaccaggc ggtgatgtcg caaaagcacc acgcgctgta agtatgctag ctaatacaac agctattgca gaagcttggg ctagacttga ttataagttt gatttaatgt atgcaaaacg tgcctttgta cattggtacg ttggagaggg tatggaagaa ggcgaattca cggaagcacg tgaagatttg gctgctttgg aaagagatta tgatgaggtt ggttttgata gtaatgatga taatgatggc gaagatgcaa tgtattga
Details for BrmaA.17143.b.B2.GE39617
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 51kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHMR EVISVHVGQA GVQIGNAAWE LFCLEHGIQP DGTTCKIQNK QHADNSFSTF FLETGTGRHV PRAILIDLEP SVIDEIRTGP YKRLFHPEQM VTGKEDAANN YARGHYTVGR ELIDVVLDRI RRLAENCTGL QGFLIFHSFG GGTGSGFTSL LMERLTTDYD KKSKLEFSIY PAPQVSTAVV EPYNSILTTH TTLEHSDCSF MVDNEAIYDI CRRNLDVERP SYLNLNRLIS QVVSSITASL RFDGALNVDL TEFQTNLVPY PRIHFPLATY APIISAEKAY HESLSVMDIT NSCFEPANQM VKCDPRLGKY MAVCLLYRGD VTSKDVNAAI NSIKTKRTIQ FVDWCPTGFK IGINYQPPTV VPGGDVAKAP RAVGFDSNDD NGGEDAMY
Validated NT Sequence
catggctcac caccaccacc accatatgcg tgaagtaatc tcagttcatg ttggacaagc tggtgtacaa attggtaatg ctgcgtggga attattttgt ttggaacatg gaatacaacc ggatggaaca acatgcaaaa tccagaataa acaacatgct gataattcat tttcaacatt cttcctagaa accggtaccg gacgacatgt accacgagcc attttaattg atcttgaacc atctgttatc gatgaaattc gaacaggacc atataaacgt ttatttcatc ctgaacaaat ggttaccggt aaagaagatg cagcaaataa ttatgcacgt ggacattata cagttggtag agaattaatt gatgttgttc tggatcgaat aagacgactt gccgaaaatt gtaccggttt acagggtttt cttatatttc attcatttgg tggtggtaca ggttccggtt ttacatcatt attaatggaa cgattaacaa ctgattatga taaaaaaagt aaattggaat tttcaatcta tccagcacca caagtatcta cagctgttgt cgaaccatat aattcaattt taaccacaca tacaacttta gagcattctg attgctcatt tatggttgat aatgaagcaa tttatgatat ctgtcgtcgt aatttagatg tagaacgacc atcatatctc aatcttaatc gtttaatttc acaggttgtt tcatcaatta cagcttcatt acgttttgat ggtgcattaa atgtagattt gacagaattt caaacaaatt tagtaccata tccacgtata cattttccat tagctactta tgctccaata atttctgctg aaaaagctta tcatgaatcg ttatctgtaa tggatatcac taattcatgc tttgaaccag ctaatcaaat ggttaaatgt gatccacgtc ttggaaaata catggcagtt tgtcttttat atcgtggtga tgtgacatct aaagatgtta atgcagcaat taatagtatt aaaacaaaac gtacaataca atttgttgat tggtgtccaa caggtttcaa aataggtatc aattatcaac caccaacagt tgtaccaggc ggtgatgtcg caaaagcacc acgcgctgtt ggttttgata gtaat
Expected Protein Sequence
MAHHHHHHMR EVISVHVGQA GVQIGNAAWE LFCLEHGIQP DGTTCKIQNK QHADNSFSTF FLETGTGRHV PRAILIDLEP SVIGPYKRLF HPEQMVTGKE DAANNYARGH YTVGRELIDV VLDRIRRLAE NCTGLQGFLI FHSFGGGTGS GFTSLLMERL TTDYDKKSKL EFSIYPAPQV STAVVEPYNS ILTTHTTLEH SDCSFMVDNE AIYDICRRNL DVERPSYLNL NRLISQVVSS ITASLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPIIS AEKAYHESLS VMDITNSCFE PANQMVKCDP RLGKYMAVCL LYRGDVTSKD VNAAINSIKT KRTIQFVDWC PTGFKIGINY QPPTVVPGGD VAKAPRAVSM LANTTAIAEA WARLDYKFDL MYAKRAFVHW YVGEGMEEGE FTEAREDLAA LERDYDEVGF DSNDDNDGED AMY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg aagtaatctc agttcatgtt ggacaagctg gtgtacaaat tggtaatgct gcgtgggaat tattttgttt ggaacatgga atacaaccgg atggaacaac atgcaaaatc cagaataaac aacatgctga taattcattt tcaacattct tcctagaaac cggtaccgga cgacatgtac cacgagccat tttaattgat cttgaaccat ctgttatcgg accatataaa cgtttatttc atcctgaaca aatggttacc ggtaaagaag atgcagcaaa taattatgca cgtggacatt atacagttgg tagagaatta attgatgttg ttctggatcg aataagacga cttgccgaaa attgtaccgg tttacagggt tttcttatat ttcattcatt tggtggtggt acaggttccg gttttacatc attattaatg gaacgattaa caactgatta tgataaaaaa agtaaattgg aattttcaat ctatccagca ccacaagtat ctacagctgt tgtcgaacca tataattcaa ttttaaccac acatacaact ttagagcatt ctgattgctc atttatggtt gataatgaag caatttatga tatctgtcgt cgtaatttag atgtagaacg accatcatat ctcaatctta atcgtttaat ttcacaggtt gtttcatcaa ttacagcttc attacgtttt gatggtgcat taaatgtaga tttgacagaa tttcaaacaa atttagtacc atatccacgt atacattttc cattagctac ttatgctcca ataatttctg ctgaaaaagc ttatcatgaa tcgttatctg taatggatat cactaattca tgctttgaac cagctaatca aatggttaaa tgtgatccac gtcttggaaa atacatggca gtttgtcttt tatatcgtgg tgatgtgaca tctaaagatg ttaatgcagc aattaatagt attaaaacaa aacgtacaat acaatttgtt gattggtgtc caacaggttt caaaataggt atcaattatc aaccaccaac agttgtacca ggcggtgatg tcgcaaaagc accacgcgct gtaagtatgc tagctaatac aacagctatt gcagaagctt gggctagact tgattataag tttgatttaa tgtatgcaaa acgtgccttt gtacattggt acgttggaga gggtatggaa gaaggcgaat tcacggaagc acgtgaagat ttggctgctt tggaaagaga ttatgatgag gttggttttg atagtaatga tgataatgat ggcgaagatg caatgtattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat