BrmaA.17143.a

tubulin beta chain, putative

CENTER ID: BrmaA.17143.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17143.a.B1.GE36391 full length 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001893005.1
UniProt: A8NQI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MREILQIQAG QCGNQIGTKF WEVISDEHGI KPDGTYHGES DLQRERINVY YNEASNGRYV PRTVLVDLEP GTMDSVRNSP YGKLFRPDNF VFGQSGAGNN WAKGHYTEGA ELVDSVLDVV RKEVEGCDCL QGFQLTHSLG GGTGSGMGTL MISKIREEYP DRIMSTFSVV PSPKVSDVVL EPYNATLSVH QLVENTDETF CIDNEALYDI CFRTLKLSDP NYNNLNHLVS MTMSGVTTCL RFPGQLNADL RKLAVNMIPF PRLHFFIPGF APLFALNSAA YRALTVAELT QQVFDAKNMM AACDPRHGRY LTVAVMFRGS MSMSEVDSQM FNVQDKNSSY FVEWIPNNVK TAVCDIPPRG LKMSATFIGN STAIMELFKR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNLNDLVS EYQQYQDATI EDDDEYNESG SDIYNNQVEP EY
NT Sequence
atgcgagaaa ttcttcaaat tcaggctggc caatgtggta atcaaattgg cacaaagttt tgggaggtga tttcggatga acatggtata aaaccagatg gcacatatca tggtgaatcg gatttgcaac gcgagcgtat taatgtttac tacaatgaag caagcaatgg tcgttacgtc cctcgcactg ttctggttga tctagaaccc ggtacaatgg attcagttcg caacagtcca tacgggaagc tctttcgtcc cgataatttt gtttttggtc aaagtggtgc tggtaacaac tgggccaaag gacattatac agaaggagca gaactagttg acagcgttct ggatgtcgtc agaaaagaag ttgaaggatg cgactgcttg cagggttttc aactcactca ttcgttggga ggcggtacag gctcaggcat gggtacgcta atgatttcaa aaattcgtga agagtatccg gaccgtatca tgagtacatt ttcagtcgtt ccatcaccaa aggtttcgga tgtcgtactg gaaccataca atgctactct atcagttcat caattagttg aaaatacaga tgagacattc tgtatcgaca atgaggcatt gtatgatatt tgcttccgaa ctttgaagtt atcagatccc aactataaca atctcaatca tttggtctca atgacaatgt ctggagtaac aacttgtctt cgttttcctg gccaacttaa tgctgacctc cgtaaattgg cggtaaatat gattccgttt ccacgtctcc atttcttcat accgggattt gcaccacttt ttgcactcaa ttcagctgcc tatcgcgctc tgaccgttgc tgaactcaca cagcaggttt tcgatgcaaa gaacatgatg gcagcatgcg atccacgaca tggccgttat cttacggtag cggttatgtt ccgtggctcg atgtcgatga gcgaagtgga cagtcagatg ttcaatgtac aggataagaa ttcatcgtat ttcgtggagt ggattccaaa caatgttaaa acagctgttt gtgatattcc accacgcggc ttaaaaatga gtgcaacttt tattggtaat tcgacagcta tcatggagct ttttaaacgt gtctcggaac agtttactgc aatgttccgt cgtaaggctt ttcttcattg gtacactggc gaagggatgg atgagatgga atttactgag gcagaaagca atttgaatga tctcgtgtcg gaatatcagc aataccagga tgctaccatt gaggatgatg atgaatacaa tgagagtggc agtgatatat ataataatca ggttgaaccg gaatattaa
Details for BrmaA.17143.a.B1.GE36391
HARVESTED ON: 11/21/2012
SEQUENCED ON: 11/27/2012
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 48
Validated AA Sequence
MAHHHHHHMR EILQIQAGQC GNQIGTKFWE VISDEHGIKP DGTYHGESDL QRERINVYYN EASNGRYVPR TVLVDLEPGT MDSVRNSPYG KLFRPDNFVF GQSGAGNNWA KGHYTEGAEL VDSVLDVVRK EVEGCDCLQG FQLTHSLGGG TGSGMGTLMI SKIREEYPDR IMSTFSVVPS PKVSDVVLEP YNATLSVHQL VXXQMRHSVS TMRHCMIFAS EL
Validated NT Sequence
ttnnnnnntt taagaaggag anataccatg gctcaccacc accaccacca tatgcgagaa attcttcaaa ttcaggctgg ccaatgtggt aatcaaattg gcacaaagtt ttgggaggtg atttcggatg aacatggtat aaaaccagat ggcacatatc atggtgaatc ggatttgcaa cgcgagcgta ttaatgttta ctacaatgaa gcaagcaatg gtcgttacgt ccctcgcact gttctggttg atctagaacc cggtacaatg gattcagttc gcaacagtcc atacgggaag ctctttcgtc ccgataattt tgtttttggt caaagtggtg ctggtaacaa ctgggccaaa ggacattata cagaaggagc agaactagtt gacagcgttc tggatgtcgt cagaaaagaa gttgaaggat gcgactgctt gcagggtttt caactcactc attcgttggg aggcggtaca ggctcaggca tgggtacgct aatgatttca aaaattcgtg aagagtatcc ggaccgtatc atgagtacat tttcagtcgt tccatcacca aaggtttcgg atgtcgtact ggaaccatac aatgctactc tatcagttca tcaattagtt nnnnnacaga tgagacattc tgtatcgacn atgaggcatt gtatgatatt tgcttccgaa ctttgaagtt atcagatccc aactataaca atctcaatca tttggtctca atgacaatgt ctggagtaac aacttgtctt cgttttcctg gccaacttaa tgctgacctc cgtaaattgg cggtaaatat gattccgttt ccncgtctcc atttcttcat accgggattt gcaccacttt ttgcnctcaa ttcagctgcc tatcgcgctc tgaccgntgc tganncncnc agcnnnttcn atgcaangaa natgangnng catgcnancn ngacatnnnn nnntnngnag nnnngnncga tgtnnnnann nannnnnnnt nnnanntcan nnnnngnann nntcnnattt nnnnnnnnnc annangnnna nnnnnnnntn nnnnnnnnnn naannnnnna nntnnnnnnn nnnnnnnnnn ngnnnttnnn nnnnnnnnan nnntnnnnnn na
Expected Protein Sequence
MAHHHHHHMR EILQIQAGQC GNQIGTKFWE VISDEHGIKP DGTYHGESDL QRERINVYYN EASNGRYVPR TVLVDLEPGT MDSVRNSPYG KLFRPDNFVF GQSGAGNNWA KGHYTEGAEL VDSVLDVVRK EVEGCDCLQG FQLTHSLGGG TGSGMGTLMI SKIREEYPDR IMSTFSVVPS PKVSDVLEPY NATLSVHQLV ENTDETFCID NEALYDICFR TLKLSDPNYN NLNHLVSMTM SGVTTCLRFP GQLNADLRKL AVNMIPFPRL HFFIPGFAPL FALNSAAYRA LTVAELTQQV FDAKNMMAAC DPRHGRYLTV AVMFRGSMSM SEVDNQMFNV QDKNSSYFVE WIPNNVKTAV CDIPPRGLKM SATFIGNSTA IMELFKRVSE QFTAMFRRKA FLHWYTGEGM DEMEFTEAES NLNDLVSEYQ QYQDATIEDD DEYNESGSDI YNNQVEPEY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag aaattcttca aattcaggct ggccaatgtg gtaatcaaat tggcacaaag ttttgggagg tgatttcgga tgaacatggt ataaaaccag atggcacata tcatggtgaa tcggatttgc aacgcgagcg tattaatgtt tactacaatg aagcaagcaa tggtcgttac gtccctcgca ctgttctggt tgatctagaa cccggtacaa tggattcagt tcgcaacagt ccatacggga agctctttcg tcccgataat tttgtttttg gtcaaagtgg tgctggtaac aactgggcca aaggacatta tacagaagga gcagaactag ttgacagcgt tctggatgtc gtcagaaaag aagttgaagg atgcgactgc ttgcagggtt ttcaactcac tcattcgttg ggaggcggta caggctcagg catgggtacg ctaatgattt caaaaattcg tgaagagtat ccggaccgta tcatgagtac attttcagtc gttccatcac caaaggtttc ggatgtygta ctggaaccat acaatgctac tctatcagtt catcaattag ttgaaaatac agatgagaca ttctgtatcg acaatgaggc attgtatgat atttgcttcc gaactttgaa gttatcagat cccaactata acaatctcaa tcatttggtc tcaatgacaa tgtctggagt aacaacttgt cttcgttttc ctggccaact taatgctgac ctccgtaaat tggcggtaaa tatgattccg tttccacgtc tccatttctt cataccggga tttgcaccac tttttgcact caattcagct gcctatcgcg ctctgaccgt tgctgaactc acacagcagg ttttcgatgc aaagaacatg atggcagcat gtgatccacg acatggccgt tatcttacgg tggcggttat gttccgtggc tcgatgtcga tgagcgaagt ggacaatcag atgttcaatg tacaggataa gaattcatcg tatttcgtgg agtggattcc aaacaatgtt aaaacagctg tttgtgatat tccaccacgc ggcttaaaaa tgagtgcaac ttttattggt aattcgacag ctatcatgga gctttttaaa cgtgtctcgg aacagtttac tgcaatgttc cgtcgtaagg cttttcttca ttggtacact ggcgaaggga tggatgagat ggaatttact gaggcagaaa gcaatttgaa tgatctcgtg tcggaatatc agcaatacca ggatgctacc attgaggatg atgatgaata caatgagagt ggcagtgata tatataataa tcaggttgaa ccggaatatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat