BrmaA.17109.a

Csnk2b protein, putative

CENTER ID: BrmaA.17109.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17109.a.B2.GE39616 casein kinase 2 (CK2) domain 8 204
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.17109.a.B2.PS37839 casein kinase 2 (CK2) domain 8 204

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001901236.1
UniProt: A8QBV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSSEEVSWI TWFCGLRGNE FFCEVDEEYI QDKFNLTGLN EQVPHYRQAL DMILDLEPDD DFEDNPNQSD LVEQAAEMLY GLIHARYILT NRGIQQMIDK WHDHDFGVCP RVYCENQPML PIGLSDVPGE SMVKLYCPRC CDVYVPKSSK HQNTDGSYFG TGFPHMLFFV HPEERPKRPA TSFVPRLYGF KIHPTAYTLQ YQQSQCNGNV NNVMSTISQC YVWSSRSRTE
NT Sequence
atgagtagtt cagaggaagt gtcatggatc acatggtttt gcggcctacg aggaaatgag ttcttttgtg aggtagacga ggaatatatc caagataagt ttaatttaac tggtctcaat gagcaagttc cacactaccg gcaagcactt gatatgattt tggatttaga accagatgac gactttgaag acaatccgaa ccagtcagat ttagttgagc aagctgctga aatgctttat ggattaattc atgctcgcta tattcttaca aatcgcggaa tccagcaaat gattgacaag tggcatgatc atgactttgg cgtatgtcct cgtgtttatt gcgagaatca accaatgcta ccaataggtc tttcggatgt acctggcgaa tcaatggtta aactctactg tcctcgttgt tgcgacgttt atgtgccaaa gtcatcaaag catcagaata ctgatggttc ttattttggt acaggatttc ctcacatgct tttctttgtg catccggaag agagaccaaa gcgacccgca acaagtttcg ttccacgatt gtatggtttc aaaatacacc ctactgcgta cactttacag taccagcaaa gccagtgcaa tggtaatgtg aacaacgtga tgtcgacaat ttcgcagtgt tatgtgtggt cttcacgaag tagaactgag tga
Details for BrmaA.17109.a.B2.GE39616
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 24kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS WITWFCGLRG NEFFCEVDEE YIQDKFNLTG LNEQVPHYRQ ALDMILDLEP DDDFEDNPNQ SDLVEQAAEM LYGLIHARYI LTNRGIQQMI DKWHDHDFGV CPRVYCENQP MLPIGLSDVP GESMVKLYCP RCCDVYVPKS SKHQNTDGSY FGTGFPHMLF FVHPEERPKR PATSFVPRLY GFKIHPTAYT LSTSKAK
Validated NT Sequence
atggctcacc accaccacca ccatatgtca tggatcacat ggttttgcgg cctacgagga aatgagttct tttgtgaggt agacgaggaa tatatccaag ataagtttaa tttaactggt ctcaatgagc aagttccaca ctaccggcaa gcacttgata tgattttgga tttagaacca gatgacgact ttgaagacaa tccgaaccag tcagatttag ttgagcaagc tgctgaaatg ctttatggat taattcatgc tcgctatatt cttacaaatc gcggaatcca gcaaatgatt gacaagtggc atgatcatga ctttggcgta tgtcctcgtg tttattgcga gaatcaacca atgctaccaa taggtctttc ggatgtacct ggcgaatcaa tggttaaact ctactgtcct cgttgttgcg acgtttatgt gccaaagtca tcaaagcatc agaatactga tggttcttat tttggtacag gatttcctca catgcttttc tttgtgcatc cggaagagag accaaagcga cccgcaacaa gtttcgttcc acgattgtat ggtttcaaaa tacaccctac tgcgtacact ttaagtacca gcaaagctaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHSW ITWFCGLRGN EFFCEVDEEY IQDKFNLTGL NEQVPHYRQA LDMILDLEPD DDFEDNPNQS DLVEQAAEML YGLIHARYIL TNRGIQQMID KWHDHDFGVC PRVYCENQPM LPIGLSDVPG ESMVKLYCPR CCDVYVPKSS KHQNTDGSYF GTGFPHMLFF VHPEERPKRP ATSFVPRLYG FKIHPTAYTL QYQQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcatgga tcacatggtt ttgcggccta cgaggaaatg agttcttttg tgaggtagac gaggaatata tccaagataa gtttaattta actggtctca atgagcaagt tccacactac cggcaagcac ttgatatgat tttggattta gaaccagatg acgactttga agacaatccg aaccagtcag atttagttga gcaagctgct gaaatgcttt atggattaat tcatgctcgc tatattctta caaatcgcgg aatccagcaa atgattgaca agtggcatga tcatgacttt ggcgtatgtc ctcgtgttta ttgcgagaat caaccaatgc taccaatagg tctttcggat gtacctggcg aatcaatggt taaactctac tgtcctcgtt gttgcgacgt ttatgtgcca aagtcatcaa agcatcagaa tactgatggt tcttattttg gtacaggatt tcctcacatg cttttctttg tgcatccgga agagagacca aagcgacccg caacaagttt cgttccacga ttgtatggtt tcaaaataca ccctactgcg tacactttac agtaccagca aagctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BrmaA.17109.a.B2.PS37839
PURIFICATION DATe: 2/17/2016
CONCENTRATION: 2.4mg/ml
OBSERVED MW: 24kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS WITWFCGLRG NEFFCEVDEE YIQDKFNLTG LNEQVPHYRQ ALDMILDLEP DDDFEDNPNQ SDLVEQAAEM LYGLIHARYI LTNRGIQQMI DKWHDHDFGV CPRVYCENQP MLPIGLSDVP GESMVKLYCP RCCDVYVPKS SKHQNTDGSY FGTGFPHMLF FVHPEERPKR PATSFVPRLY GFKIHPTAYT LSTSKAK
Validated NT Sequence
atggctcacc accaccacca ccatatgtca tggatcacat ggttttgcgg cctacgagga aatgagttct tttgtgaggt agacgaggaa tatatccaag ataagtttaa tttaactggt ctcaatgagc aagttccaca ctaccggcaa gcacttgata tgattttgga tttagaacca gatgacgact ttgaagacaa tccgaaccag tcagatttag ttgagcaagc tgctgaaatg ctttatggat taattcatgc tcgctatatt cttacaaatc gcggaatcca gcaaatgatt gacaagtggc atgatcatga ctttggcgta tgtcctcgtg tttattgcga gaatcaacca atgctaccaa taggtctttc ggatgtacct ggcgaatcaa tggttaaact ctactgtcct cgttgttgcg acgtttatgt gccaaagtca tcaaagcatc agaatactga tggttcttat tttggtacag gatttcctca catgcttttc tttgtgcatc cggaagagag accaaagcga cccgcaacaa gtttcgttcc acgattgtat ggtttcaaaa tacaccctac tgcgtacact ttaagtacca gcaaagctaa gtgagtaaga t
Expressed Protein Sequence
MAHHHHHHSW ITWFCGLRGN EFFCEVDEEY IQDKFNLTGL NEQVPHYRQA LDMILDLEPD DDFEDNPNQS DLVEQAAEML YGLIHARYIL TNRGIQQMID KWHDHDFGVC PRVYCENQPM LPIGLSDVPG ESMVKLYCPR CCDVYVPKSS KHQNTDGSYF GTGFPHMLFF VHPEERPKRP ATSFVPRLYG FKIHPTAYTL QYQQS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTCATGGA TCACATGGTT TTGCGGCCTA CGAGGAAATG AGTTCTTTTG TGAGGTAGAC GAGGAATATA TCCAAGATAA GTTTAATTTA ACTGGTCTCA ATGAGCAAGT TCCACACTAC CGGCAAGCAC TTGATATGAT TTTGGATTTA GAACCAGATG ACGACTTTGA AGACAATCCG AACCAGTCAG ATTTAGTTGA GCAAGCTGCT GAAATGCTTT ATGGATTAAT TCATGCTCGC TATATTCTTA CAAATCGCGG AATCCAGCAA ATGATTGACA AGTGGCATGA TCATGACTTT GGCGTATGTC CTCGTGTTTA TTGCGAGAAT CAACCAATGC TACCAATAGG TCTTTCGGAT GTACCTGGCG AATCAATGGT TAAACTCTAC TGTCCTCGTT GTTGCGACGT TTATGTGCCA AAGTCATCAA AGCATCAGAA TACTGATGGT TCTTATTTTG GTACAGGATT TCCTCACATG CTTTTCTTTG TGCATCCGGA AGAGAGACCA AAGCGACCCG CAACAAGTTT CGTTCCACGA TTGTATGGTT TCAAAATACA CCCTACTGCG TACACTTTAC AGTACCAGCA AAGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT