BrmaA.01418.a

cyclophilin B, putative

CENTER ID: BrmaA.01418.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.01418.a.B4.GE39481 cyclophilin-like domain 163 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001901410.1
UniProt: A8QCK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHLITFSSSL VEHYFFDGLN SSSAAYFCRR SLHKFRMMKT DGYVFLFMHL VLCLLQTPTS IIWRAAVINL GSISHVMDHI SLSRCHHICT NPSIRCGGVR LRSMQHTVII SAYLRIALCS TVDCIFGNNL LSGSPVYPFT DFFMTWQYRS LLLLCLLNVY ATLKSDNEPK GPKVTDKVIL TIKIGDEEAG IITIGLFGKT VPKTVKNFIQ LSKKAEGEGY TGSKFHRIIK DFMVQAGDFI NGDGTGSISI YGKKFADENF KLKHYGAGWV SMANAGPDSN GSQFFITLKK TPWLDGRHVV FGKVLSGMKV VRKMENTKTL KPSDKPVEDI IIIKAEHIIV DAPFSVHKTD AED
NT Sequence
atgcatctta taacgttttc ctcgtcttta gtggagcatt atttttttga tggcttgaac tcttcctctg cggcgtactt ctgtagaaga tcgcttcaca aattcagaat gatgaaaact gatggatatg tattcctgtt tatgcatttg gtgctttgct tactgcaaac tccaacaagc attatatggc gtgcagctgt gatcaacctt ggtagcattt ctcatgttat ggaccacata tcacttagca gatgtcatca catctgtacg aatccttcga tcagatgtgg gggtgtacgt ttacgcagca tgcagcatac cgtcatcata tctgcttatt tgcgaatagc gctttgtagt actgtcgatt gcatttttgg gaataatcta ctctctggtt ctcccgttta ccccttcacg gattttttta tgacttggca gtacagatca ctactgctgt tatgtctatt gaatgtttat gcaacactga aatcagacaa cgaaccgaaa ggaccaaaag taactgacaa ggtaattttg acgataaaaa ttggagatga agaagcaggc ataataacaa ttggcttatt tggaaaaaca gttccgaaaa ctgtaaaaaa ttttatccag ctatcaaaga aagcagaagg agaaggatat actggctcaa aattccatcg aattatcaaa gatttcatgg tacaggctgg cgacttcatt aatggtgacg gtaccggcag tatatctatc tatggtaaaa agttcgccga tgaaaatttc aaattgaagc attacggtgc aggatgggtt agcatggcaa atgctggacc agattcgaat ggttctcagt tcttcattac tttaaaaaaa acaccctggc tcgatggcag acacgtcgtt ttcggcaagg tcctcagcgg aatgaaagta gtaagaaaaa tggaaaatac gaaaacatta aaacctagtg acaagcccgt agaggatatc atcatcataa aagcagaaca tattattgta gatgctccat tttcagtcca caagactgac gcagaagatt aa
Details for BrmaA.01418.a.B4.GE39481
HARVESTED ON: 9/10/2015
SEQUENCED ON: 9/16/2015
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHML KSDNEPKGPK VTDKVILTIK IGDEEAGIIT IGLFGKTVPK TVKNFIQLSK KAEGEGYTGS KFHRIIKDFM VQAGDFINGD GTGSISIYGK KFADENFKLK HYGAGWVSMA NAGPDSNGSQ FFITLKKTPW LDGRHVVFGK VRELIIFVSF FVASFQLLPF QVLSGMKVVR KMENTKTLKP SDKPVEDIII IKAEHIIVDA PFSVHK
Validated NT Sequence
atcttactca cttacttgtg gactgaaaat ggagcatcta caataatatg ttctgctttt atgatgatga tatcctctac gggcttgtca ctaggtttta atgttttcgt attttccatt tttcttacta ctttcattcc gctgaggacc tggaaaggaa gaagttgaaa agaagccaca aagaacgaaa caaaaataat tagctctcgt accttgccga aaacgacgtg tctgccatcg agccagggtg ttttttttaa agtaatgaag aactgagaac cattcgaatc tggtccagca tttgccatgc taacccatcc tgcaccgtaa tgcttcaatt tgaaattttc atcggcgaac tttttaccat agatagatat actgccggta ccgtcaccat taatgaagtc gccagcctgt accatgaaat ctttgataat tcgatggaat tttgagccag tatatccttc tccttctgct ttctttgata gctggataaa attttttaca gttttcggaa ctgtttttcc aaataagcca attgttatta tgcctgcttc ttcatctcca atttttatcg tcaaaattac cttgtcagtt acttttggtc ctttcggttc gttgtctgat ttcagcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHLK SDNEPKGPKV TDKVILTIKI GDEEAGIITI GLFGKTVPKT VKNFIQLSKK AEGEGYTGSK FHRIIKDFMV QAGDFINGDG TGSISIYGKK FADENFKLKH YGAGWVSMAN AGPDSNGSQF FITLKKTPWL DGRHVVFGKV LSGMKVVRKM ENTKTLKPSD KPVEDIIIIK AEHIIVDAPF SVHK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgaaat cagacaacga accgaaagga ccaaaagtaa ctgacaaggt aattttgacg ataaaaattg gagatgaaga agcaggcata ataacaattg gcttatttgg aaaaacagtt ccgaaaactg taaaaaattt tatccagcta tcaaagaaag cagaaggaga aggatatact ggctcaaaat tccatcgaat tatcaaagat ttcatggtac aggctggcga cttcattaat ggtgacggta ccggcagtat atctatctat ggtaaaaagt tcgccgatga aaatttcaaa ttgaagcatt acggtgcagg atgggttagc atggcaaatg ctggaccaga ttcgaatggt tctcagttct tcattacttt aaaaaaaaca ccctggctcg atggcagaca cgtcgttttc ggcaaggtcc tcagcggaat gaaagtagta agaaaaatgg aaaatacgaa aacattaaaa cctagtgaca agcccgtaga ggatatcatc atcataaaag cagaacatat tattgtagat gctccatttt cagtccacaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat