SSGCID: Target Page

BrmaA.01255.b

transglutaminase, putative

CENTER ID:	BrmaA.01255.b
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.01255.b.B3.GE39614	ordered region		30	595

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001900589.1
UniProt:	A8Q7P0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLRISLILI YLLECVSVEE KESEFQQNGG VFVLNERNFM SFLQQHPTAL VEFYAPWCGH CKALAPEYAK AAKKLKVPLA KVDATVETKL AETYNIEEFP TLKFWQNDKD PIVYDGGLES NEIIQWVLEK TDPTYKAPPL AVAKLTKEKF SGFITLHQLV LVKFYAPWCG HCRKLAPEYE KAARKLKSAG IMLAEVDSTV EKSLSAEFDI TGYPTLYIFR NGKKFDYKGP RDTEGIVKHM LEQAEPALRK INSVKEAQHF MRKDDITVIG FFSDGKAELL ESLSEAAEMV RNDFSIAVCL QVNTKKYFKI DSDQIVIFFP EIYWSKYESK QIVYQKACDG TVEDLVRFFQ ENSTPLVGQR TKKNVATRYT KLPLVVIYYN IDFSVEYREG TQYWRKKVLD IANEYRKHKY HFAISDESEF ADELIAVGLG DSGLEHNVLV FGYDGKKYPM RPNEFDDELS ENLQAFMKKL SSGKIKPFMK SAPLPKDNKG PVKTVVASNF AQVVFDETKD VLMEFYAPWC GLCKAFESKY KELAVKLKSE SNLLLVKIDA TANDIPKNYD VSGFPTIYFA PAGKKKEPIK YKGNRDLDDL INFMKKHASI SFRSKIEL

NT Sequence

atgctgttaa ggataagtct aatcctgata tatctgcttg agtgtgtatc tgtggaagag aaagaaagcg aatttcaaca aaatggtggt gtctttgttt tgaatgagag aaatttcatg tccttccttc agcaacatcc aacagcacta gtagaatttt atgctccctg gtgtggccac tgtaaagcgc tggcaccgga gtatgcaaag gctgctaaga aactgaaggt gccattggct aaagtggatg ctaccgttga aacaaaactt gcagaaactt acaacatcga agaatttcca acactaaaat tttggcaaaa tgacaaagat ccaatcgttt acgatggagg attagaatct aacgagatta tacagtgggt tttggagaaa acagatccca cttataaggc acctccgcta gctgttgcga aattaacgaa agaaaaattt agtggattta tcactttaca ccaactcgta ttagtcaaat tctatgcgcc atggtgtggt cattgtagga aactcgcacc tgaatatgaa aaagctgcga gaaaattaaa aagtgcaggt ataatgcttg cagaagtgga ttctacagtt gagaagagtt tatcagcgga atttgatatc actggctatc caacattgta catcttccga aacggaaaga aatttgatta caaaggacct cgtgatacag agggtattgt gaaacacatg cttgaacagg ctgaacctgc tttaagaaaa attaacagtg tgaaggaggc tcagcatttt atgcgcaaag atgatataac agtcattggt tttttcagtg atggaaaagc agaactactg gaatctttaa gtgaagcagc tgaaatggtg cgaaatgatt tcagtattgc tgtttgtttg caagtgaaca ctaaaaaata ttttaaaatt gattctgacc aaatagttat ttttttccct gaaatatact ggagcaaata cgagtcgaag caaattgttt accaaaaggc atgcgatggt actgtggaag atttggtaag attttttcaa gaaaattcaa cacctcttgt gggtcaaaga accaaaaaaa atgttgccac cagatatact aaactccctc ttgttgtaat ttattataat attgacttct cagtagaata tcgcgaagga acacaatact ggaggaaaaa ggtgttagat attgcaaatg aatataggaa acataagtat cattttgcaa tttcagacga aagcgagttt gcagatgagc ttatagcggt gggattgggt gacagtgggc ttgagcataa tgttttagtt ttcggttatg atggtaaaaa gtacccgatg cgtccgaatg aattcgatga tgaattatct gagaatttac aagctttcat gaaaaaacta tcttcaggta aaataaaacc ttttatgaaa tctgcgccat tgccaaaaga taataagggt ccagtgaaaa ctgtcgttgc atcaaatttc gctcaggttg tgtttgatga aacgaaggat gtgctgatgg agttttatgc accgtggtgt gggctttgta aggcattcga gtcaaaatac aaagagttgg cagtgaaatt aaaaagtgaa tcaaatttgc tacttgtaaa aattgatgca acagccaatg atattccgaa gaattacgat gtgagtggtt ttccaacaat ttattttgca ccagctggta aaaagaagga accaatcaaa tacaaaggaa atcgtgactt agatgacctc attaacttca tgaaaaagca tgcctctatt tcgtttcgaa gtaaaatcga attgtag

Details for BrmaA.01255.b.B3.GE39614

HARVESTED ON:	10/30/2015
SEQUENCED ON:	10/23/2015
EXPECTED MW:	66kDa
OBSERVED MW:	73kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGV FVLNERNFMS FLQQHPTALV EFYAPWCGHC KALAPEYAKA AKKLKVPLAK VDATVETKLA ETYNIEEFPT LKFWQNDKDP IVYDGGLESN EIIQWVLEKT DPTYKAPPLA VAKLTKEKFS GFITLHQLVL VKFYAPWCGH CRKLAPEYEK AARKLKSAGI MLAEVDSTVE KSLSAEFDIT GYPTLYIFRN GKKFDYKGPR DTEGIVKHML EQAEPALRKI NSVKEAQHFM RKDDITVIGF FSDGKAELLE SLSEAAEMVR NDFSIAVCLQ VNTKKYFKID SDQIVIFFPE IYWSKYESKQ IVYQKACDGT VEDLVRFFQE NSTPLVGQRT KKNVATRYTK LPLVVIYYNI DFSVEYREGT QYWRKKVLDI ANEYRKHKYH FAISDESEFA DELIAVGLGD SGLEHNVLVF GYDGKKYPMR PNEFDDELSE NLQAFMKKLS SGKIKPFMKS APLPKDNKGP VKTVVASNFA QVVFDETKDV LMEFYAPWCG LCKAFESKYK ELAVKLKSES NLLLVKIDAT ANDIPKNYDV SGFPTIYFAP AGKKKEPIKY KGNRDLDDLI NFMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtgtct ttgttttgaa tgagagaaat ttcatgtcct tccttcagca acatccaaca gcactagtag aattttatgc tccctggtgt ggccactgta aagcgctggc accggagtat gcaaaggctg ctaagaaact gaaggtgcca ttggctaaag tggatgctac cgttgaaaca aaacttgcag aaacttacaa catcgaagaa tttccaacac taaaattttg gcaaaatgac aaagatccaa tcgtttacga tggaggatta gaatctaacg agattataca gtgggttttg gagaaaacag atcccactta taaggcacct ccgctagctg ttgcgaaatt aacgaaagaa aaatttagtg gatttatcac tttacaccaa ctcgtattag tcaaattcta tgcgccatgg tgtggtcatt gtaggaaact cgcacctgaa tatgaaaaag ctgcgagaaa attaaaaagt gcaggtataa tgcttgcaga agtggattct acagttgaga agagtttatc agcggaattt gatatcactg gctatccaac attgtacatc ttccgaaacg gaaagaaatt tgattacaaa ggacctcgtg atacagaggg tattgtgaaa cacatgcttg aacaggctga acctgcttta agaaaaatta acagtgtgaa ggaggctcag cattttatgc gcaaagatga tataacagtc attggttttt tcagtgatgg aaaagcagaa ctactggaat ctttaagtga agcagctgaa atggtgcgaa atgatttcag tattgctgtt tgtttgcaag tgaacactaa aaaatatttt aaaattgatt ctgaccaaat agttattttt ttccctgaaa tatactggag caaatacgag tcgaagcaaa ttgtttacca aaaggcatgc gatggtactg tggaagattt ggtaagattt tttcaagaaa attcaacacc tcttgtgggt caaagaacca aaaaaaatgt tgccaccaga tatactaaac tccctcttgt tgtaatttat tataatattg acttctcagt agaatatcgc gaaggaacac aatactggag gaaaaaggtg ttagatattg caaatgaata taggaaacat aagtatcatt ttgcaatttc agacgaaagc gagtttgcag atgagcttat agcggtggga ttgggtgaca gtgggcttga gcataatgtt ttagttttcg gttatgatgg taaaaagtac ccgatgcgtc cgaatgaatt cgatgatgaa ttatctgaga atttacaagc tttcatgaaa aaactatctt caggtaaaat aaaacctttt atgaaatctg cgccattgcc aaaagataat aagggtccag tgaaaactgt cgttgcatca aatttcgctc aggttgtgtt tgatgaaacg aaggatgtgc tgatggagtt ttatgcaccg tggtgtgggc tttgtaaggc attcgagtca aaatacaaag agttggcagt gaaattaaaa agtgaatcaa atttgctact tgtaaaaatt gatgcaacag ccaatgatat tccgaagaat tacgatgtga gtggttttcc aacaatttat tttgcaccag ctggtaaaaa gaaggaacca atcaaataca aaggaaatcg tgacttagat gacctcatta acttcatgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat