BrmaA.01238.b

Seryl-tRNA synthetase, cytoplasmic (SerRS), putative

CENTER ID: BrmaA.01238.b
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.01238.b.B1.GE41315 full length 1 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0H5SAX5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLDIDLFRE EKGGDPELVR DSQRKRYKDV KLVDDVIDYD QRWRRDRFST DNFNRLINLC SKVIGEKMKK KESVADDNTV PIDYMERLEA LCADDIKGLK ISQIKKLKNT IDQKLVEKRR EMEKLEAKRH QRLIQIGNII HPTVPVSDNE DNNRVEKTFG DIKTKKKYSH IDLCMMIDGF DGDRGTSVAG GRGYFLKGPL VFLEQAVINF ALQMLHCKGF IALYTPFFMR KEVMQEVAQL NQFDQELYKV TGKSSEVIGD ETNDEKYLIA TSEQPIAAYH RNEWISEADL PIKYAGISSC FRQEVGSHGR DTRGMFRVHQ FEKVEQFVIC SPYDDESWRI LDEMVENAED FCKALGIPFR IVCIVSGELN NAAAKKLDLE AWFPGSAAFR ELVSCSNCTD YQARRLKIRY GKTKKLDGEV SYVHMLNGTM CATTRVLCAL LENYQEENGI RVPEILRQFM PHPYKELIPF IKEAPIENDL KKIN
NT Sequence
atggttctag acattgattt gtttcgagaa gagaaaggcg gtgatccgga actggtacga gattcgcaac gaaaacgata taaggatgtc aaacttgttg acgacgttat tgattatgat caacgatgga gaagagatcg tttttccaca gataatttca acaggttgat caatttatgc agcaaagtca ttggagaaaa aatgaagaaa aaagaatcag tagctgatga taataccgta ccaatcgatt atatggaacg cctagaagca ctttgtgctg atgacataaa gggtttgaaa atatcacaaa tcaaaaaact aaagaataca attgatcaga agctggtgga aaaaaggaga gaaatggaga agcttgaagc aaaacgccat caaaggctta ttcagatagg caacattata catcccacgg tgccggtcag tgataacgaa gacaataata gagtggagaa gacttttggt gacatcaaaa ctaaaaagaa atactcacat atagatcttt gtatgatgat tgatgggttt gatggtgatc gtggaacttc tgtcgctggt ggtcgcggtt attttttgaa aggtccgtta gtatttttgg agcaagcagt tatcaatttt gctttacaaa tgctacactg taaaggcttc attgcgcttt acactccatt cttcatgcgt aaagaagtga tgcaagaagt ggcacaactt aatcagtttg atcaggagtt atacaaggtt actgggaaaa gttctgaagt tatcggcgac gaaaccaatg acgagaaata cctcatagct acatccgagc agccaattgc agcttatcac cgaaacgaat ggattagtga agctgacttg ccaattaaat atgccggcat atcgtcttgt ttccgccaag aggttggaag tcatgggcga gatactcgtg gtatgtttcg agtacatcaa tttgaaaaag ttgagcaatt tgtcatatgt tctccatatg atgatgaaag ttggagaata cttgatgaga tggttgaaaa tgctgaagat ttctgtaaag cacttggtat accatttcga attgtatgca ttgtttcggg agaactaaat aatgctgcgg cgaaaaagct tgatttagaa gcgtggtttc caggcagtgc cgcattcaga gaattggttt cttgttcgaa ttgtaccgat tatcaagctc gacgtttgaa aattcgttat gggaaaacga aaaagttgga tggtgaagta tcttacgtac atatgttgaa tggtacaatg tgtgcaacta cacgtgtatt atgtgcgtta ttggaaaatt atcaagaaga aaatggtata cgtgttccgg aaattctaag acaatttatg ccacatccct acaaggaatt aataccattc attaaggaag caccgattga aaatgattta aagaaaataa attaa
Details for BrmaA.01238.b.B1.GE41315
HARVESTED ON: 3/31/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 57kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV LDIDFFREEK GGDPELVRDS QRKRYKDVKL VDDVIDYDQR WRRDRFSTDN FNRLINLCSK VIGEKMKKKE SVADDNTVPI DYMERLEALC ADDIKGLKIS QIKKLKNTID QKLVEKRREM EKLEAKRHQR LIQIGNIIHP TVPVSDNEDN NRVEKTFGDI KTKKKYSHID LCMMIDGFDG DRGTSVAGGR GYFLKGPLVF LEQAVINFAL QMLHCKGFIA LYTPFFMRKE VMQEVAQLNQ FDQELYKVTG KSSEVIGDET NDEKYLIATS EQPIAAYHRN EWISEADLPI KYAGISSCFR QEVGSHGRDT RGMFRVHQFE KVEQFVICSP YDDESWRILD EMVENAEDFC KALGIPFRIV CIVSGELNNA AAKKLDLEAW FPGSAAFREL VSCSNCTDYQ ARRLKIRYGK TKKLDGEVSY VHMLNGTMCA TTRVLCALLE NYQEENGIRV PEILRQFMPH PYKELIPFIK EAPX
Validated NT Sequence
cnatcggtgc ttccttaatg aatggtatta attccttgta gggatgtggc ataaattgtc ttagaatttc cggaacacgt ataccatttt cttcttgata attttccagt aacgcacata atacacgtgt agttgcacac attgtaccat tcaacatatg tacgtaagat acttcaccat ccaacttttt cgttttccca taacgaattt tcaaacgtcg agcttgataa tcggtacaat tcgaacaaga aaccaattct ctgaatgcgg cactgcctgg aaaccacgct tctaaatcaa gctttttcgc cgcagcatta tttagttctc ccgaaacaat gcatacaatt cgaaatggta taccaagtgc tttacagaaa tcttcagcat tttcaaccat ctcatcaagt attctccaac tttcatcatc atatggagaa catatgacaa attgctcaac tttttcaaat tgatgtactc gaaacatacc acgagtatct cgcccatgac ttccaacctc ttggcggaaa caagacgata tgccggcata tttaattggc aagtcagctt cactaatcca ttcgtttcgg tgataagctg caattggctg ctcggatgta gctatgaggt atttctcgtc attggtttcg tcgccgataa cttcagaact tttcccagta accttgtata actcctgatc aaactgatta agttgtgcca cttcttgcat cacttcttta cgcatgaaga atggagtgta aagcgcaatg aagcctttac agtgtagcat ttgtaaagca aaattgataa ctgcttgctc caaaaatact aacggacctt tcaaaaaata accgcgacca ccagcgacag aagttccacg atcaccatca aacccatcaa tcatcataca aagatctata tgtgagtatt tctttttagt tttgatgtca ccaaaagtct tctccactct attattgtct tcgttatcac tgaccggcac cgtgggatgt ataatgttgc ctatctgaat aagcctttga tggcgttttg cttcaagctt ctccatttct ctcctttttt ccaccagctt ctgatcaatt gtattcttta gttttttgat ttgtgatatt ttcaaaccct ttatgtcatc agcacaaagt gcttctaggc gttccatata atcgattggt acggtattat catcagctac tgattctttt ttcttcattt tttctccaat gactttgctg cataaattga tcaacctgtt gaaattatct gtggaaaaac gatctcttct ccatcgttga tcataatcaa taacgtcgtc aacaagtttg acatccttat atcgttttcg ttgcgaatct cgtaccagtt ccggatcacc gcctttctct tctcgaaaaa aatcaatgtc tagaaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMV LDIDLFREEK GGDPELVRDS QRKRYKDVKL VDDVIDYDQR WRRDRFSTDN FNRLINLCSK VIGEKMKKKE SVADDNTVPI DYMERLEALC ADDIKGLKIS QIKKLKNTID QKLVEKRREM EKLEAKRHQR LIQIGNIIHP TVPVSDNEDN NRVEKTFGDI KTKKKYSHID LCMMIDGFDG DRGTSVAGGR GYFLKGPLVF LEQAVINFAL QMLHCKGFIA LYTPFFMRKE VMQEVAQLNQ FDQELYKVTG KSSEVIGDET NDEKYLIATS EQPIAAYHRN EWISEADLPI KYAGISSCFR QEVGSHGRDT RGMFRVHQFE KVEQFVICSP YDDESWRILD EMVENAEDFC KALGIPFRIV CIVSGELNNA AAKKLDLEAW FPGSAAFREL VSCSNCTDYQ ARRLKIRYGK TKKLDGEVSY VHMLNGTMCA TTRVLCALLE NYQEENGIRV PEILRQFMPH PYKELIPFIK EAPIENDLKK IN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttc tagacattga tttgtttcga gaagagaaag gcggtgatcc ggaactggta cgagattcgc aacgaaaacg atataaggat gtcaaacttg ttgacgacgt tattgattat gatcaacgat ggagaagaga tcgtttttcc acagataatt tcaacaggtt gatcaattta tgcagcaaag tcattggaga aaaaatgaag aaaaaagaat cagtagctga tgataatacc gtaccaatcg attatatgga acgcctagaa gcactttgtg ctgatgacat aaagggtttg aaaatatcac aaatcaaaaa actaaagaat acaattgatc agaagctggt ggaaaaaagg agagaaatgg agaagcttga agcaaaacgc catcaaaggc ttattcagat aggcaacatt atacatccca cggtgccggt cagtgataac gaagacaata atagagtgga gaagactttt ggtgacatca aaactaaaaa gaaatactca catatagatc tttgtatgat gattgatggg tttgatggtg atcgtggaac ttctgtcgct ggtggtcgcg gttatttttt gaaaggtccg ttagtatttt tggagcaagc agttatcaat tttgctttac aaatgctaca ctgtaaaggc ttcattgcgc tttacactcc attcttcatg cgtaaagaag tgatgcaaga agtggcacaa cttaatcagt ttgatcagga gttatacaag gttactggga aaagttctga agttatcggc gacgaaacca atgacgagaa atacctcata gctacatccg agcagccaat tgcagcttat caccgaaacg aatggattag tgaagctgac ttgccaatta aatatgccgg catatcgtct tgtttccgcc aagaggttgg aagtcatggg cgagatactc gtggtatgtt tcgagtacat caatttgaaa aagttgagca atttgtcata tgttctccat atgatgatga aagttggaga atacttgatg agatggttga aaatgctgaa gatttctgta aagcacttgg tataccattt cgaattgtat gcattgtttc gggagaacta aataatgctg cggcgaaaaa gcttgattta gaagcgtggt ttccaggcag tgccgcattc agagaattgg tttcttgttc gaattgtacc gattatcaag ctcgacgttt gaaaattcgt tatgggaaaa cgaaaaagtt ggatggtgaa gtatcttacg tacatatgtt gaatggtaca atgtgtgcaa ctacacgtgt attatgtgcg ttattggaaa attatcaaga agaaaatggt atacgtgttc cggaaattct aagacaattt atgccacatc cctacaagga attaatacca ttcattaagg aagcaccgat tgaaaatgat ttaaagaaaa taaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat