BrmaA.01122.a

Probable dimethyladenosine transferase, putative

CENTER ID: BrmaA.01122.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.01122.a.B1.GE36319 full length 1 364
BrmaA.01122.a.B2.GE39476 folded domain 13 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001893303.1
UniProt: A8NT02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATNFSRLPP LPSLKSFLYA YQLQAKKILS QNYLMDMNLT RKIVRQAEMK KGDYVVEIGP GPGSITRAIL EMDCRRLDVI EIDHRFVPPL EVLKEASEER MFIHRADILK TNIEQIWSQA GLERVAWEED RLPTAHIIGN LPFNIASPLI IKFLREMLYR QGPWSFGRVP LLLTFQMEVA ERLCSPIDSP FRARISIMSA FVTEPKLLFQ IPGRCFVPSP KVNVGVVRFV PRQDPLIKTS FEVVEKVCRR IFNYRQKYVI KGVRSLYPKE LAKHLADDLL KRCRIDPTTT AICLGVEQFA DICYVYEEHC RKYPGVFLYE HSNQNRTLEE LARLPNAIPP PNPFDKEFPS EGVKLSDALA LFGE
NT Sequence
atggcgacga atttttcgcg cttaccacct ttaccatcat tgaaaagctt cctttatgcc tatcaattac aagccaaaaa aattctttca caaaattatt taatggatat gaatttaaca cggaagatag taaggcaagc ggaaatgaaa aaaggcgact atgtagtcga gattggacct ggcccaggat cgatcactcg tgctatttta gaaatggatt gtcgacgact tgatgtcatc gaaatcgacc atcgattcgt tccaccactt gaagtgctaa aggaagcaag cgaagaacgt atgttcattc atcgagctga tattttaaaa acaaatattg aacaaatttg gtctcaagct ggtttggagc gtgttgcttg ggaggaggat agacttccga cggcgcatat tattggcaac cttcctttca atattgcttc tccacttatt ataaaattcc ttcgtgaaat gttatatcgc caaggaccat ggtcatttgg tcgtgtccca ttgctgctaa cgtttcagat ggaagtagct gaacgtctct gttccccaat tgattctcca tttcgagcta gaatttcaat catgtctgca tttgtaaccg aaccaaaatt gctttttcaa ataccaggaa gatgctttgt tccatctcct aaagttaatg tcggtgtggt acgttttgtt ccaagacagg acccattgat taaaacatcc tttgaggtag tagaaaaagt ttgccgacgt atttttaact acaggcaaaa atatgttatc aagggtgttc gaagtcttta tcctaaagaa ttagccaaac atttggccga tgatttgttg aaacgatgta gaattgatcc tacaactact gcaatatgtc ttggagtaga gcagtttgct gacatttgct atgtttatga ggaacactgc cgaaaatatc ctggtgtatt tctatatgaa cattcaaatc aaaatcgtac tctggaagaa cttgcacgac ttccaaatgc tataccacca ccaaatcctt ttgataaaga atttccatca gaaggtgtga aattgtcaga tgctttagca ttatttggtg agtag
Details for BrmaA.01122.a.B1.GE36319
HARVESTED ON: 10/23/2012
SEQUENCED ON: 10/23/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMA TNFSRLPPLP SLKSFLYAYQ LQAKKILSQN YLMDMNLTRK IVRQAEMKKG DYVVEIGPGP GSITRAILEM DCRRLDVIEI DHRFVPPLEV LKEASEERMF IHRADILKTN IEQIWSQAGL ERVAWEEDRL PTAHIIGNLP FNIASPLIIK FLREMLYRQG PWSFGRVPLL LTFQMEVAER LCSPIDSPFR ARISIMSAFV TEPKLLFQIP GRCFVPSPKV NVGVVRFVPR QDPLIKTSFE VVEKVCRRIF NYXQKYVIKG VRTLYPKELA KHLADDLLKR CRXDPTT
Validated NT Sequence
atggctcacc accaccacca ccatatggcg acgaattttt cgcgcttacc acctttacca tcattgaaaa gcttccttta tgcctatcaa ttacaagcca aaaaaattct ttcacaaaat tatttaatgg atatgaattt aacacggaag atagtaaggc aagcggaaat gaaaaaaggc gactatgtag tcgagattgg acctggccca ggatcgatca ctcgtgctat tttagaaatg gattgtcgac gacttgatgt catcgaaatc gaccatcgat tcgttccacc acttgaagtg ctaaaggaag caagcgaaga acgtatgttc attcatcgag ctgatatttt aaaaacaaat attgaacaaa tttggtctca agctggtttg gagcgtgttg cttgggagga ggatagactt ccgacggcgc atattattgg caaccttcct ttcaatattg cttctccact tattataaaa ttccttcgtg aaatgttata tcgccaagga ccatggtcat ttggtcgtgt cccattgctg ctaacgtttc agatggaagt agctgaacgt ctctgttctc caattgattc tccatttcga gctagaattt caatcatgtc tgcatttgta accgaaccaa aattgctttt tcaaatacca ggaagatgct ttgttccatc tcctaaagtt aatgtcggtg tggtacgttt tgttccaaga caggacccat tgattaaaac atcctttgag gtagtagaaa aagtttgccg acgtattttt aactacnggc aaaaatatgt tatcaagggt gttcgaactc tttatcctaa agaattagcc aaacatttgg ccgatgattt gttgaaacga tgtagaantg atcctacaac tac
Expected Protein Sequence
MAHHHHHHMA TNFSRLPPLP SLKSFLYAYQ LQAKKILSQN YLMDMNLTRK IVRQAEMKKG DYVVEIGPGP GSITRAILEM DCRRLDVIEI DHRFVPPLEV LKEASEERMF IHRADILKTN IEQIWSQAGL ERVAWEEDRL PTAHIIGNLP FNIASPLIIK FLREMLYRQG PWSFGRVPLL LTFQMEVAER LCPIDSPFRA RISIMSAFVT EPKLLFQIPG RCFVPSPKVN VGVVRFVPRQ DPLIKTSFEV VEKVCRRIFN YRQKYVIKGV RTLYPKELAK HLADDLLKRC RIDPTTTAIC LGVEQFADIC YVYEEHCRKY PGVFLYEHSN QNRTLEELAR LPNAIPPPNP FDKEFPSEGV KLSDALALFG E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga cgaatttttc gcgcttacca cctttaccat cattgaaaag cttcctttat gcctatcaat tacaagccaa aaaaattctt tcacaaaatt atttaatgga tatgaattta acacggaaga tagtaaggca agcggaaatg aaaaaaggcg actatgtagt cgagattgga cctggcccag gatcgatcac tcgtgctatt ttagaaatgg attgtcgacg acttgatgtc atcgaaatcg accatcgatt cgttccacca cttgaagtgc taaaggaagc aagcgaagaa cgtatgttca ttcatcgagc tgatatttta aaaacaaata ttgaacaaat ttggtctcaa gctggtttgg agcgtgttgc ttgggaggag gatagacttc cgacggcgca tattattggc aaccttcctt tcaatattgc ttctccactt attataaaat tccttcgtga aatgttatat cgccaaggac catggtcatt tggtcgtgtc ccattgctgc taacgtttca gatggaagta gctgaacgtc tctgttcycc aattgattct ccatttcgag ctagaatttc aatcatgtct gcatttgtaa ccgaaccaaa attgcttttt caaataccag gaagatgctt tgttccatct cctaaagtta atgtcggtgt ggtacgtttt gttccaagac aggacccatt gattaaaaca tcctttgagg tagtagaaaa agtttgccga cgtattttta actacaggca aaaatatgtt atcaagggtg ttcgaactct ttatcctaaa gaattagcca aacatttggc cgatgatttg ttgaaacgat gtagaattga tcctacaact actgcaatat gtcttggagt agagcagttt gctgacattt gctatgttta tgaggaacac tgccgaaaat atcctggtgt atttctatat gaacattcaa atcaaaatcg tactctggaa gaacttgcac gacttccaaa tgctatacca ccaccaaatc cttttgataa agaatttcca tcagaaggtg tgaaattgtc agatgcttta gcattatttg gtgagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BrmaA.01122.a.B2.GE39476
HARVESTED ON: data unavailable
SEQUENCED ON: 9/16/2015
EXPECTED MW: 37kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LKSFLYAYQL QAKKILSQNY LMDMNLTRKI VRQAEMKKGD YVVEIGPGPG SITRAILEMD CRRLDVIEID HRFVPPLEVL KEASEERMFI HRADILKTNI EQIWSQAGLE RVAWEEDRLP TAHIIGNLPF NIASPLIIKF LREMLYRQGP WSFGRVPLLL TFQMEVAERL CSPIDSPFRA RISIMSAFVT EPKLLFQIPG RCFVPSPKVN VGVVRFVPRQ DPLIKTSFEV VEKVCRRIFN YRQKYVIKGV RSLYPKELAK HLADDLLKRC RIDPTTTAIC LGVEQFADIC YVYEEHCRKX XXVFLYE
Validated NT Sequence
tcttactcac ttattcatat agaaatacac nnnnntattt tcggcagtgt tcctcataaa catagcaaat gtcagcaaac tgctctactc caagacatat tgcagtagtt gtaggatcaa ttctacatcg tttcaacaaa tcatcggcca aatgtttggc taattcttta ggataaagac ttcgaacacc cttgataaca tatttttgcc tgtagttaaa aatacgtcgg caaacttttt ctactacctc aaaggatgtt ttaatcaatg ggtcctgtct tggaacaaaa cgtaccacac cgacattaac tttaggagat ggaacaaagc atcttcctgg tatttgaaaa agcaattttg gttcggttac aaatgcagac atgattgaaa ttctagctcg aaatggagaa tcaattgggg aacagagacg ttcagctact tccatctgaa acgttagcag caatgggaca cgaccaaatg accatggtcc ttggcgatat aacatttcac gaaggaattt tataataagt ggagaagcaa tattgaaagg aaggttgcca ataatatgcg ccgtcggaag tctatcctcc tcccaagcaa cacgctccaa accagcttga gaccaaattt gttcaatatt tgtttttaaa atatcagctc gatgaatgaa catacgttct tcgcttgctt cctttagcac ttcaagtggt ggaacgaatc gatggtcgat ttcgatgaca tcaagtcgtc gacaatccat ttctaaaata gcacgagtga tcgatcctgg gccaggtcca atctcgacta catagtcgcc ttttttcatt tccgcttgcc ttactatctt ccgtgttaaa ttcatatcca ttaaataatt ttgtgaaaga atttttttgg cttgtaattg ataggcataa aggaagcttt tcaatgacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHSL KSFLYAYQLQ AKKILSQNYL MDMNLTRKIV RQAEMKKGDY VVEIGPGPGS ITRAILEMDC RRLDVIEIDH RFVPPLEVLK EASEERMFIH RADILKTNIE QIWSQAGLER VAWEEDRLPT AHIIGNLPFN IASPLIIKFL REMLYRQGPW SFGRVPLLLT FQMEVAERLC SPIDSPFRAR ISIMSAFVTE PKLLFQIPGR CFVPSPKVNV GVVRFVPRQD PLIKTSFEVV EKVCRRIFNY RQKYVIKGVR SLYPKELAKH LADDLLKRCR IDPTTTAICL GVEQFADICY VYEEHCRKYP GVFLYE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcattga aaagcttcct ttatgcctat caattacaag ccaaaaaaat tctttcacaa aattatttaa tggatatgaa tttaacacgg aagatagtaa ggcaagcgga aatgaaaaaa ggcgactatg tagtcgagat tggacctggc ccaggatcga tcactcgtgc tattttagaa atggattgtc gacgacttga tgtcatcgaa atcgaccatc gattcgttcc accacttgaa gtgctaaagg aagcaagcga agaacgtatg ttcattcatc gagctgatat tttaaaaaca aatattgaac aaatttggtc tcaagctggt ttggagcgtg ttgcttggga ggaggataga cttccgacgg cgcatattat tggcaacctt cctttcaata ttgcttctcc acttattata aaattccttc gtgaaatgtt atatcgccaa ggaccatggt catttggtcg tgtcccattg ctgctaacgt ttcagatgga agtagctgaa cgtctctgtt ccccaattga ttctccattt cgagctagaa tttcaatcat gtctgcattt gtaaccgaac caaaattgct ttttcaaata ccaggaagat gctttgttcc atctcctaaa gttaatgtcg gtgtggtacg ttttgttcca agacaggacc cattgattaa aacatccttt gaggtagtag aaaaagtttg ccgacgtatt tttaactaca ggcaaaaata tgttatcaag ggtgttcgaa gtctttatcc taaagaatta gccaaacatt tggccgatga tttgttgaaa cgatgtagaa ttgatcctac aactactgca atatgtcttg gagtagagca gtttgctgac atttgctatg tttatgagga acactgccga aaatatcctg gtgtatttct atatgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at