SSGCID: Target Page

BrmaA.01054.a

proteasome subunit beta type 1, putative

CENTER ID:	BrmaA.01054.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.01054.a.B1.GE36314	full length		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001894290.1
UniProt:	A8P1E7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGLMDELDMG SYPGGNMKAF KKKLAEVHAS PPWMTPVLKE ASEWNPYSFE GGTTAAIAGD NFAIMASDTR MSQFEINVLT RDAEKIHVLN DSIVLSCAGF YGDILQLKRL LEARLHKYRF DYRGDMTVDL CAELLSRNLY YRRFFPYYTG AILAGIDENG KGAVFSYDPI GCIEHIAYTA SGSAEPMIMP FFDCQIGLVT MSGDVEKPLL TIERATSLIK DAFRVAAERE ISTGDKIHLV IAEAGKPIRK MHLPLRED

NT Sequence

atgggtctta tggatgaatt agatatgggc tcctatcctg gtggaaacat gaaagctttt aaaaagaagc tagccgaagt ccatgcatca ccaccttgga tgaccccagt attgaaggaa gcatcggaat ggaatcctta ctctttcgaa ggaggaacta cagcagcaat tgctggtgat aactttgcga ttatggcgag tgatacacgc atgtcacaat ttgaaatcaa tgttttgact cgtgatgcag agaagatcca tgtcttaaat gattcaattg tactttcgtg tgctggattt tacggcgata ttctgcagct gaagcgattg ttggaggctc gtttacataa gtatcggttt gattatcgtg gggatatgac cgtagattta tgcgccgagt tattatcacg aaatttatat tatcgacgtt tttttccgta ttacaccggt gcaatattag ccggcattga tgagaatggt aaaggagctg tgtttagcta tgatcccatt gggtgcattg aacatatcgc gtacactgct tccggtagcg ctgaaccaat gattatgccg tttttcgatt gccagattgg tttggtaaca atgtctggtg atgtggagaa gccgcttttg acaattgaac gagctacatc acttataaaa gatgcttttc gtgtagcagc agaacgtgaa ataagcacag gagataagat acatttggtg attgctgaag caggcaaacc aattcgcaag atgcatttgc ctcttcgtga agattaa

Details for BrmaA.01054.a.B1.GE36314

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG LMDELDMGSY PGGNMKAFKK KLAEVHASPP WMTPVLKEAS EWNPYSFEGG TTAAIAGDNF AIMASDTRMS QFEINVLTRD AEKIHVLNDS IVLSCAGFYG DILQLKRLLE ARLHKYRFDY RGDMTVDLCA ELLSRNLYYR RFFPYYTGAI LAGIDENGKG AVFSYDPIGC IEHIAYTASG SAEPMIMPFF DCQIGLVTMS GDVEKPLLTI ERATSLIKXA FRVAAEREIS TGXXIHLVIA EXXNQFARCI CLXVXXX

Validated NT Sequence

ttntttanct ttaagaagga gatataccat ggctcaccac caccaccacc atatgggtct tatggatgaa ttagatatgg gctcctatcc tggtggaaac atgaaagctt ttaaaaagaa gctagccgaa gtccatgcat caccaccttg gatgacccca gtattgaagg aagcatcgga atggaatcct tactctttcg aaggaggaac tacagcagca attgctggtg ataactttgc gattatggcg agtgatacac gcatgtcaca atttgaaatc aatgttttga ctcgtgatgc agagaagatc catgtcttaa atgattcaat tgtactttcg tgtgctggat tttacggcga tattctgcag ctgaagcgat tgttggaggc tcgtttacat aagtatcggt ttgattatcg tggggatatg accgtagatt tatgcgccga gttattatca cgaaatttat attatcgacg tttttttccg tattacaccg gtgcaatatt agccggcatt gatgagaatg gtaaaggagc tgtgtttagc tatgatccca ttgggtgcat tgaacatatc gcgtacactg cttccggtag cgctgaacca atgattatgc cgtttttcga ttgccagatt ggtttggtaa caatgtctgg tgatgtggag aagccgcttt tgacaattga acgagctaca tcacttataa aanangcttt tcgtgtagca gcagaacgtg aaataagcac aggaganaan atacatttgg tgattgctga ancngnaaac caattcgcaa gatgcatttg cctcntcgtg aanantaant gannanatag gannnntncn ananaccccn naannnnnnn naaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc ttatggatga attagatatg ggctcctatc ctggtggaaa catgaaagct tttaaaaaga agctagccga agtccatgca tcaccacctt ggatgacccc agtattgaag gaagcatcgg aatggaatcc ttactctttc gaaggaggaa ctacagcagc aattgctggt gataactttg cgattatggc gagtgataca cgcatgtcac aatttgaaat caatgttttg actcgtgatg cagagaagat ccatgtctta aatgattcaa ttgtactttc gtgtgctgga ttttacggcg atattctgca gctgaagcga ttgttggagg ctcgtttaca taagtatcgg tttgattatc gtggggatat gaccgtagat ttatgcgccg agttattatc acgaaattta tattatcgac gtttttttcc gtattacacc ggtgcaatat tagccggcat tgatgagaat ggtaaaggag ctgtgtttag ctatgatccc attgggtgca ttgaacatat cgcgtacact gcttccggta gcgctgaacc aatgattatg ccgtttttcg attgccagat tggtttggta acaatgtctg gtgatgtgga gaagccgctt ttgacaattg aacgagctac atcacttata aaagatgctt ttcgtgtagc agcagaacgt gaaataagca caggagataa gatacatttg gtgattgctg aagcaggcaa accaattcgc aagatgcatt tgcctcttcg tgaagattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at