BrmaA.00975.a

inosine-5-monophosphate dehydrogenase, putative

CENTER ID: BrmaA.00975.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.00975.a.B2.GE39611 ordered region 9 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001897693.1
UniProt: A8PQ14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDGDNAIRIN EDGQTIDELM SNNVGLTYND FNILPGYIGF DVSSVDLTTH LTRGITLKTP LVSSPMDTVT ECEMAIAMAL HGGIGIIHAN FASVEDQAEE VIKVKRYKQG FITHPHCIKE MDTVLDLMRI KLKYGFTGTP VTSTGHVGGQ LIGLVTSRDV DFIDESKYPT TKISEVMVPF DRLITGSEDL TLEHAYKILE NEKKGKLPIV NSRNELVSLI ARTDLKKARD FPCSSYDSKG QLRVGAAINT RESAKEAVKK LVAAGADVLV IDSSQGASMY QVNLLKWIKT NYPETPQIIA GNVVTQRQAE ILINAGADAI RVGMGSGSIC ITQEVTAVGR AQGTAVYQVA KYARTRGIPV IADGGIRDVG YITKALALGA STVMMGGLLA GTTEAPGEYF WGPSGVRLKN YRGMGSLDAM EANVGSQDRY FSSRSDSIKV AQGVSATMRD RGSIHKFVPY LVRGIQHGFQ DIGVKNLDEL RNGIARGEVR FERRSSNAQV EGGVHSLHSY DKRLY
NT Sequence
atggatggtg acaatgctat cagaattaat gaagatgggc agacaattga tgaacttatg agcaataatg ttgggttgac atacaatgat ttcaatatat tgccgggtta tatcggtttt gacgtttctt ccgtggattt aacaacgcat ttaactcgtg gtattacgct taaaacacca ttggtgtcat caccgatgga tacagtaacc gaatgcgaaa tggcgattgc tatggcatta catggtggta ttggtataat tcacgccaat ttcgcctccg tggaagatca ggctgaagaa gttatcaaag tgaagcgtta taagcaaggg tttataacac atccgcattg cataaaagaa atggatacag tgttagattt gatgaggata aagctgaaat atggtttcac aggaacacca gttacgtcaa caggacatgt tggtggccaa ctgataggtt tggtaacttc tcgtgatgta gattttattg atgagagcaa atatccgact acgaaaataa gtgaagtaat ggtcccattc gaccggctta taacgggcag cgaagatcta actttagaac atgcttacaa aatcttagaa aatgagaaga aagggaaact acctattgtg aacagcagaa acgaactggt atccttaata gctcgtactg atttaaagaa agctcgagat tttccatgta gttcttacga ttcgaaggga cagttaagag tgggcgcagc tataaatacc cgagaatccg caaaagaagc ggtaaagaaa ttagttgcgg ctggtgctga tgttcttgtc attgattctt cacaaggtgc cagcatgtat caagtgaatc ttttgaagtg gataaagacc aattatccag agacgccaca gataattgca ggcaatgttg taacacagag gcaagcagaa atcctaatca atgctggggc agatgccatt cgcgttggta tgggaagtgg ttcaatttgt attactcaag aagttactgc agttggacga gcacaaggca cagcggtgta tcaagttgca aaatacgcta gaactcgtgg gattcccgtc atcgctgatg gtggaataag ggatgttggt tacatcacga aggcattggc acttggtgca tcgacagtga tgatgggtgg tcttttagca ggcacaacag aagcacccgg agaatatttc tggggtccaa gcggtgtgcg tcttaaaaat tatcgtggaa tgggttcact ggatgctatg gaggcaaatg tcggtagtca agataggtat tttagcagta ggtcagattc gataaaggtt gcgcaggggg tgtctgctac aatgagggat agaggtagca tccacaaatt cgttccatat ttggtgcgtg gaattcaaca cggctttcag gatatcggtg tgaaaaattt agatgaactc aggaatggta ttgctcgtgg tgaagtcaga tttgaacgtc gatcatcgaa tgctcaagtt gagggaggag tgcattcctt gcactcttac gataaacgtc tttattga
Details for BrmaA.00975.a.B2.GE39611
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI NEDGQTIDEL MSNNVGLTYN DFNILPGYIG FDVSSVDLTT HLTRGITLKT PLVSSPMDTV TECEMAIAMA LHGGIGIIHA NFASVEDQAE EVIKVKRYKQ GFITHPHCIK EMDTVLDLMR IKLKYGFTGT PVTSTGHVGG QLIGLVTSRD VDFIDESKYP TTKISEVMVP FDRLITGSED LTLEHAYKIL ENEKKGKLPI VNSRNELVSL IARTDLKKAR DFPCSSYDSK GQLRVGAAIN TRESAKEAVK KLVAAGADVL VIDSSQGASM YQVNLLKWIK TNYPETPQII AGNVVTQRQA EILINAGADA IRVGMGSGSI CITQEVTAVG RAQGTAVYQV AKYARTRGIP VIADGGIRDV GYITKALALG ASTVMMGGLL AGTTEAPGEY FWGPSGVRLK NYRGMGSLDA MEANVGSQDR YFSSRSDSIK VAQGVSATMR DRGSIHKFVP YLVRGIQHGF QDIGVKNLDE LRNGIARGEV RFE
Validated NT Sequence
ccatggctca ccaccaccac caccatatga ttaatgaaga tgggcagaca attgatgaac ttatgagcaa taatgttggg ttgacataca atgatttcaa tatattgccg ggttatatcg gttttgacgt ttcttccgtg gatttaacaa cgcatttaac tcgtggtatt acgcttaaaa caccattggt gtcatcaccg atggatacag taaccgaatg cgaaatggcg attgctatgg cattacatgg tggtattggt ataattcacg ccaatttcgc ctccgtggaa gatcaggctg aagaagttat caaagtgaag cgttataagc aagggtttat aacacatccg cattgcataa aagaaatgga tacagtgtta gatttgatga ggataaagct gaaatatggt ttcacaggaa caccagttac gtcaacagga catgttggtg gccaactgat aggtttggta acttctcgtg atgtagattt tattgatgag agcaaatatc cgactacgaa aataagtgaa gtaatggtcc cattcgaccg gcttataacg ggcagcgaag atctaacttt agaacatgct tacaaaatct tagaaaatga gaagaaaggg aaactaccta ttgtgaacag cagaaacgaa ctggtatcct taatagctcg tactgattta aagaaagctc gagattttcc atgtagttct tacgattcga agggacagtt aagagtgggc gcagctataa atacccgaga atccgcaaaa gaagcggtaa agaaattagt tgcggctggt gctgatgttc ttgtcattga ttcttcacaa ggtgccagca tgtatcaagt gaatcttttg aagtggataa agaccaatta tccagagacg ccacagataa ttgcaggcaa tgttgtaaca cagaggcaag cagaaatcct aatcaatgct ggggcagatg ccattcgcgt tggtatggga agtggttcaa tttgtattac tcaagaagtt actgcagttg gacgagcaca aggcacagcg gtgtatcaag ttgcaaaata cgctagaact cgtgggattc ccgtcatcgc tgatggtgga ataagggatg ttggttacat cacgaaggca ttggcacttg gtgcatcgac agtgatgatg ggtggtcttt tagcaggcac aacagaagca cccggagaat atttctgggg tccaagcggt gtgcgtctta aaaattatcg tggaatgggt tcactggatg ctatggaggc aaatgtcggt agtcaagata ggtattttag cagtaggtca gattcgataa aggttgcgca gggggtgtct gctacaatga gggatagagg tagcatccac aaattcgttc catatttggt gcgtggaatt caacacggct ttcaggatat cggtgtgaaa aatttagatg aactcaggaa tggtattgct cgtggtgaag tcagatttga acgt
Expected Protein Sequence
MAHHHHHHIN EDGQTIDELM SNNVGLTYND FNILPGYIGF DVSSVDLTTH LTRGITLKTP LVSSPMDTVT ECEMAIAMAL HGGIGIIHAN FASVEDQAEE VIKVKRYKQG FITHPHCIKE MDTVLDLMRI KLKYGFTGTP VTSTGHVGGQ LIGLVTSRDV DFIDESKYPT TKISEVMVPF DRLITGSEDL TLEHAYKILE NEKKGKLPIV NSRNELVSLI ARTDLKKARD FPCSSYDSKG QLRVGAAINT RESAKEAVKK LVAAGADVLV IDSSQGASMY QVNLLKWIKT NYPETPQIIA GNVVTQRQAE ILINAGADAI RVGMGSGSIC ITQEVTAVGR AQGTAVYQVA KYARTRGIPV IADGGIRDVG YITKALALGA STVMMGGLLA GTTEAPGEYF WGPSGVRLKN YRGMGSLDAM EANVGSQDRY FSSRSDSIKV AQGVSATMRD RGSIHKFVPY LVRGIQHGFQ DIGVKNLDEL RNGIARGEVR FERRSSNAQV EGG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattaatg aagatgggca gacaattgat gaacttatga gcaataatgt tgggttgaca tacaatgatt tcaatatatt gccgggttat atcggttttg acgtttcttc cgtggattta acaacgcatt taactcgtgg tattacgctt aaaacaccat tggtgtcatc accgatggat acagtaaccg aatgcgaaat ggcgattgct atggcattac atggtggtat tggtataatt cacgccaatt tcgcctccgt ggaagatcag gctgaagaag ttatcaaagt gaagcgttat aagcaagggt ttataacaca tccgcattgc ataaaagaaa tggatacagt gttagatttg atgaggataa agctgaaata tggtttcaca ggaacaccag ttacgtcaac aggacatgtt ggtggccaac tgataggttt ggtaacttct cgtgatgtag attttattga tgagagcaaa tatccgacta cgaaaataag tgaagtaatg gtcccattcg accggcttat aacgggcagc gaagatctaa ctttagaaca tgcttacaaa atcttagaaa atgagaagaa agggaaacta cctattgtga acagcagaaa cgaactggta tccttaatag ctcgtactga tttaaagaaa gctcgagatt ttccatgtag ttcttacgat tcgaagggac agttaagagt gggcgcagct ataaataccc gagaatccgc aaaagaagcg gtaaagaaat tagttgcggc tggtgctgat gttcttgtca ttgattcttc acaaggtgcc agcatgtatc aagtgaatct tttgaagtgg ataaagacca attatccaga gacgccacag ataattgcag gcaatgttgt aacacagagg caagcagaaa tcctaatcaa tgctggggca gatgccattc gcgttggtat gggaagtggt tcaatttgta ttactcaaga agttactgca gttggacgag cacaaggcac agcggtgtat caagttgcaa aatacgctag aactcgtggg attcccgtca tcgctgatgg tggaataagg gatgttggtt acatcacgaa ggcattggca cttggtgcat cgacagtgat gatgggtggt cttttagcag gcacaacaga agcacccgga gaatatttct ggggtccaag cggtgtgcgt cttaaaaatt atcgtggaat gggttcactg gatgctatgg aggcaaatgt cggtagtcaa gataggtatt ttagcagtag gtcagattcg ataaaggttg cgcagggggt gtctgctaca atgagggata gaggtagcat ccacaaattc gttccatatt tggtgcgtgg aattcaacac ggctttcagg atatcggtgt gaaaaattta gatgaactca ggaatggtat tgctcgtggt gaagtcagat ttgaacgtcg atcatcgaat gctcaagttg agggaggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat