BrmaA.00879.a

Beta-centractin, putative

CENTER ID: BrmaA.00879.a
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.00879.a.B1.GE36305 full length 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: XP_001900845.1
UniProt: A8Q9C7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSYDVIANQ PVVIDNGSGV IKAGFAGDQT PKCRFANFVG RPKHTRVMAG ALEGDLFIGP KAQEYRGLLS LKYPMEHGIV TDWNDMERVW QYIYSKDQLQ IFPEEHPVLL TEAPLNPLKN REKSAEIFFE TFNIPALHIQ MQAVLSLYST GRTTGVVLDS GDGVTHVVPI FEGFAIQHGI ERMDVAGRDV TRYLRLLLRK EGANFHRSSE FEIVREIKEK MCYIATNAAK EESSESEKTA YKLPDGSTLE IGPARFRAAE ILFRPDIIGE EWPGIANALD LSIRRCEIDL RKNLYSNIVL SGGSTMFAGF GDRLLAETRR LAPKDVKIRI SAPQERLYGT WIGGSILASL DTFKKMWVSK KEYEDEGKKV LHRKTF
NT Sequence
atggattcct atgatgtaat agcaaatcaa ccagttgtta tcgataatgg gtctggggta ataaaggctg ggtttgctgg tgaccagacg ccaaagtgtc gttttgctaa ttttgttgga cgaccaaagc atacaagagt aatggctggt gctctggagg gtgacttgtt cataggtccg aaagcacaag agtatcgagg actgctgtcg ttgaaatatc caatggagca tggcatagta acggattgga atgacatgga acgcgtatgg cagtatattt atagtaaaga tcagttgcag atattcccag aagaacatcc agtgttgctt acagaagcac ctcttaatcc acttaaaaac cgagaaaaat cagctgaaat tttcttcgaa acttttaata tacctgcact gcacatccaa atgcaagctg tgctttcgct ttattcaaca ggtcgtacaa caggtgtcgt tttagactca ggagatggtg ttacacatgt tgtacctatc tttgagggtt ttgctataca gcacggaatt gagagaatgg atgtcgcagg acgagacgtg accagatatc ttcgtctctt acttcgcaaa gaaggagcta actttcatcg ttcatcagaa tttgaaattg tacgggaaat taaggaaaaa atgtgttaca ttgctacaaa tgctgcaaaa gaagaaagta gcgaaagcga gaaaaccgct tataaactgc ccgatggttc gacacttgaa attggaccag caagatttcg tgctgctgag atactgtttc ggccagacat tattggcgag gaatggccag gcattgcaaa tgctctcgat ttgtccatta gacgttgtga aatagatctg cggaaaaatc tgtatagtaa tattgtatta agtggtggat caaccatgtt cgctggattt ggggatcgat tattagctga aacgcgtcgt ttggcaccaa aagacgtgaa gattcggata tctgcaccgc aggagcggtt gtacggtaca tggataggag gttcaattct agccagtctt gatactttca agaagatgtg ggttagcaaa aaggaatatg aagacgaagg gaagaaagtg ctacatagga agacattctg a
Details for BrmaA.00879.a.B1.GE36305
HARVESTED ON: 10/23/2012
SEQUENCED ON: 10/26/2012
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 75
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMD SYDVIANQPV VIDNGSGVIK AGFAGDQTPK CRFANFVGRP KHTRVMAGAL EGDLFIGPKA QEYRGLLSLK YPMEHGIVTD WNDMERVWQY IYSKDQLQIF PEEHPVLLTE APLNPLKNRE KSAEIFFETF NIPALHIQMQ AVLSLYSTGR TTGVVLDSGD GVTHVVPIFE GFAIQHGIER MDVAGRDVTR YLRLLLRKEG ANFHRSSEFE IVREIKEKMC YIATNAAKEE SSESEKTAYK LPDGSTLEIG PARFRAAEIL FXPDIXGRXX XQALQMLSIC PLXVVXXXCX KSVXXXXXSX XXXXXXGXDX XEXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
cnnnnnnnnt tttntnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggattc ctatgatgta atagcaaatc aaccagttgt tatcgataat gggtctgggg taataaaggc tgggtttgct ggtgaccaga cgccaaagtg tcgttttgct aattttgttg gacgaccaaa gcatacaaga gtaatggctg gtgctctgga gggtgacttg ttcataggtc cgaaagcaca agagtatcga ggactgctgt cgttgaaata tccaatggag catggcatag taacggattg gaatgacatg gaacgcgtat ggcagtatat ttatagtaaa gatcagttgc agatattccc agaagaacat ccagtgttgc ttacagaagc acctcttaat ccacttaaaa accgagaaaa atcagctgaa attttcttcg aaacttttaa tatacctgca ctgcacatcc aaatgcaagc tgtgctttcg ctttattcaa caggtcgtac aacaggtgtc gttttagact caggagatgg tgttacacat gttgtaccta tctttgaggg ttttgctata cagcacggaa ttgagagaat ggatgtcgca ggacgagacg tgaccagata tcttcgtctc ttacttcgca aagaaggagc taactttcat cgttcatcag aatttgaaat tgtacgggaa attaaggaaa aaatgtgtta cattgctaca aatgctgcaa aagaagaaag tagcgaaagc gagaaaaccg cttataaact gcccgatggt tcgacacttg aaattggacc agcaagattt cgtgctgctg agatactgtt tcngccagac attantgggc gagnnanggn ccaggcattg caaatgctct cgatttgtcc attanacgtt gtgaantnnn nctgcngaaa atctgtanag nannntgnan tnagtnnnng nncnnnngnn cncttngggn tcgatntnnc tgaacncnnn ntnnnnnnaa nnnntnnnnt nnnntgnncn ncngnnnnnn ncgnnnnngn nnnnngtnnn nnnnagcntn nnnnntnnnn nannnnnngn nnnncnannn nnnnnnnnnn nngnnnnnnn nnna
Expected Protein Sequence
MAHHHHHHMD SYDVIANQPV VIDNGSGVIK AGFAGDQTPK CRFANFVGRP KHTRVMAGAL EGDLFIGPKA QEYRGLLSLK YPMEHGIVTD WNDMERVWQY IYSKDQLQIF PEEHPVLLTE APLNPLKNRE KSAEIFFETF NIPALHIQMQ AVLSLYSTGR TTGVVLDSGD GVTHVVPIFE GFAIQHGIER MDVAGRDVTR YLRLLLRKEG ANFHRSSEFE IVREIKEKMC YIATNAAKEE SSESEKTAYK LPDGSTLEIG PARFRAAEIL FRPDIIGEEW PGIANALDLS IRRCEIDLRK NLYSNIVLSG GSTMFAGFGD RLLAETRRLA PKDVKIRISA PQERLYGTWI GGSILASLDT FKKMWVSKKE YEDEGKKVLH RKTF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt cctatgatgt aatagcaaat caaccagttg ttatcgataa tgggtctggg gtaataaagg ctgggtttgc tggtgaccag acgccaaagt gtcgttttgc taattttgtt ggacgaccaa agcatacaag agtaatggct ggtgctctgg agggtgactt gttcataggt ccgaaagcac aagagtatcg aggactgctg tcgttgaaat atccaatgga gcatggcata gtaacggatt ggaatgacat ggaacgcgta tggcagtata tttatagtaa agatcagttg cagatattcc cagaagaaca tccagtgttg cttacagaag cacctcttaa tccacttaaa aaccgagaaa aatcagctga aattttcttc gaaactttta atatacctgc actgcacatc caaatgcaag ctgtgctttc gctttattca acaggtcgta caacaggtgt cgttttagac tcaggagatg gtgttacaca tgttgtacct atctttgagg gttttgctat acagcacgga attgagagaa tggatgtcgc aggacgagac gtgaccagat atcttcgtct cttacttcgc aaagaaggag ctaactttca tcgttcatca gaatttgaaa ttgtacggga aattaaggaa aaaatgtgtt acattgctac aaatgctgca aaagaagaaa gtagcgaaag cgagaaaacc gcttataaac tgcccgatgg ttcgacactt gaaattggac cagcaagatt tcgtgctgct gagatactgt ttcggccaga cattattggc gaggaatggc caggcattgc aaatgctctc gatttgtcca ttagacgttg tgaaatagat ctgcggaaaa atctgtatag taatattgta ttaagtggtg gatcaaccat gttcgctgga tttggggatc gattattagc tgaaacgcgt cgtttggcac caaaagacgt gaagattcgg atatctgcac cgcaggagcg gttgtacggt acatggatag gaggttcaat tctagccagt cttgatactt tcaagaagat gtgggttagc aaaaaggaat atgaagacga agggaagaaa gtgctacata ggaagacatt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat