BrmaA.00855.a

Glyceraldehyde 3-phosphate dehydrogenase, putative

CENTER ID:	BrmaA.00855.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00855.a.B1.GE36304	full length		1	339

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

4K9D

DEPOSITED: 4/19/2013

DETERMINATION: XRay

Diffraction Image

CLONE: BrmaA.00855.a.B1.GE36304

PROTEIN: BrmaA.00855.a.B1.PW36954

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126628
RefSeq:	XP_001899849.1
UniProt:	A8Q3K8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKPKVGING FGRIGRLVLR AAVEKDTVDV VAVNDPFINI DYMVYMFKYD STHGRFKGSV SAEGGKLIVT NGKTTHHISV HNSKDPAEIP WGVDGAEYVV ESTGVFTTTD KASAHLKGGA KKVIISAPSA DAPMFVMGVN NDTYDKANNH IISNASCTTN CLAPLAKVIH DKFGIIEGLM TTVHATTATQ KTVDGPSGKL WRDGRGAGQN IIPASTGAAK AVGKVIPDLN GKLTGMAFRV PTPDVSVVDL TCRLQKGATM DEIKAAVKEA ANGPMKGILE YTEDQVVSTD FTGDTHSSIF DALACISLNP NFVKLIAWYD NEYGYSNRVV DLISYIASR

NT Sequence

atgagcaaac caaaggttgg aatcaatgga tttggtcgca tcggtcgtct tgtcttgaga gcggcagttg aaaaagacac cgttgatgta gtggcggtca atgatccgtt tattaacatt gactatatgg tgtacatgtt caaatatgat tcaacacatg gacgcttcaa gggaagtgtt tctgcagagg gaggcaagct tattgtaaca aatggcaaaa cgacccatca tatctctgtg cacaacagca aagatcccgc tgagattcca tggggagtag acggagcgga gtatgttgtg gaatcgacgg gcgtttttac aacaacggac aaagcaagtg cgcatctgaa gggtggtgcc aagaaagtta tcatttcggc tccatctgct gatgcaccga tgtttgtaat gggtgtcaac aatgatacgt atgataaggc caacaatcat atcatctcta atgcttcttg caccaccaat tgtttggctc cgttggccaa ggtcattcat gacaaattcg gaattattga gggccttatg accactgtac atgcaacaac ggctactcag aagactgttg atggaccatc tggaaaactg tggcgagatg gtcgtggtgc tggccaaaac atcattccag caagcactgg tgcagccaag gccgtaggga aagttattcc ggatctgaat ggaaagctta ctggcatggc tttccgtgtt ccaactccgg atgtatcagt agttgatctc acttgccgac tccagaaagg tgcaacaatg gatgaaatta aggctgctgt aaaggaagca gctaatggac cgatgaaggg gattttggaa tacaccgaag accaggttgt atcaactgat ttcactggtg atacacattc atcgatcttc gatgccctgg catgcatttc tctaaatcca aatttcgtta agctgattgc atggtacgat aatgaatatg gctacagcaa ccgtgttgtt gacctcatct cgtacattgc cagcagataa

Details for BrmaA.00855.a.B1.GE36304

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

tcaacaacac ggttgctgta gccatattca ttatcgtacc atgcaatcag cttaacgaaa tttggattta gagaaatgca tgccagggca tcgaagatcg atgaatgtgt atcaccagtg aaatcagttg atacaacctg gtcttcggtg tattccaaaa tccccttcat cggtccatta gctgcttcct ttacagcagc cttaatttca tccattgttg cacctttctg gagtcggcaa gtgagatcaa ctactgatac atccggagtt ggaacacgga aagccatgcc agtaagcttt ccattcagat ccggaataac tttccctacg gccttggctg caccagtgct tgctggaatg atgttttggc cagcaccacg accatctcgc cacagttttc cagatggtcc atcaacagtc ttctgagtag ccgttgttgc atgtacagtg gtcataaggc cctcaataat tccgaatttg tcatgaatga ccttggccaa cggagccaaa caattggtgg tgcaagaagc attagagatg atatgattgt tggccttatc atacgtatca ttgttgacac ccattacaaa catcggtgca tcagcagatg gagccgaaat gataactttc ttggcaccac ccttcagatg cgcacttgct ttgtccgttg ttgtaaaaac gcccgtcgat tccacaacat actccgctcc gtctactccc catggaatct cagcgggatc tttgctgttg tgcacagaga tatgatgggt cgttttgcca tttgttacaa taagcttgcc tccctctgca gaaacacttc ccttgaagcg tccatgtgtt gaatcatatt tgaacatgta caccatatag tcaatgttaa taaacggatc attgaccgcc actacatcaa cggtgtcttt ttcaactgcc gctctcaaga caagacgacc gatgcgacca aatccattga ttccaacctt tggtttgctc atatggtggt ggtg

Expected Protein Sequence

MAHHHHHHMS KPKVGINGFG RIGRLVLRAA VEKDTVDVVA VNDPFINIDY MVYMFKYDST HGRFKGSVSA EGGKLIVTNG KTTHHISVHN SKDPAEIPWG VDGAEYVVES TGVFTTTDKA SAHLKGGAKK VIISAPSADA PMFVMGVNND TYDKANNHII SNASCTTNCL APLAKVIHDK FGIIEGLMTT VHATTATQKT VDGPSGKLWR DGRGAGQNII PASTGAAKAV GKVIPDLNGK LTGMAFRVPT PDVSVVDLTC RLQKGATMDE IKAAVKEAAN GPMKGILEYT EDQVVSTDFT GDTHSSIFDA LACISLNPNF VKLIAWYDNE YGYSNRVVDL ISYIASR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca aaccaaaggt tggaatcaat ggatttggtc gcatcggtcg tcttgtcttg agagcggcag ttgaaaaaga caccgttgat gtagtggcgg tcaatgatcc gtttattaac attgactata tggtgtacat gttcaaatat gattcaacac atggacgctt caagggaagt gtttctgcag agggaggcaa gcttattgta acaaatggca aaacgaccca tcatatctct gtgcacaaca gcaaagatcc cgctgagatt ccatggggag tagacggagc ggagtatgtt gtggaatcga cgggcgtttt tacaacaacg gacaaagcaa gtgcgcatct gaagggtggt gccaagaaag ttatcatttc ggctccatct gctgatgcac cgatgtttgt aatgggtgtc aacaatgata cgtatgataa ggccaacaat catatcatct ctaatgcttc ttgcaccacc aattgtttgg ctccgttggc caaggtcatt catgacaaat tcggaattat tgagggcctt atgaccactg tacatgcaac aacggctact cagaagactg ttgatggacc atctggaaaa ctgtggcgag atggtcgtgg tgctggccaa aacatcattc cagcaagcac tggtgcagcc aaggccgtag ggaaagttat tccggatctg aatggaaagc ttactggcat ggctttccgt gttccaactc cggatgtatc agtagttgat ctcacttgcc gactccagaa aggtgcaaca atggatgaaa ttaaggctgc tgtaaaggaa gcagctaatg gaccgatgaa ggggattttg gaatacaccg aagaccaggt tgtatcaact gatttcactg gtgatacaca ttcatcgatc ttcgatgccc tggcatgcat ttctctaaat ccaaatttcg ttaagctgat tgcatggtac gataatgaat atggctacag caaccgtgtt gttgacctca tctcgtacat tgccagcaga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat