SSGCID: Target Page

BrmaA.00825.b

Asparagine--tRNA ligase (EC 6.1.1.22)

CENTER ID:	BrmaA.00825.b
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00825.b.UX2.GE45546	2xti domain		112	539

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A4E9FJW9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVYICPETG DDGNDGSELK PLRTLYQAMI ITKSSKGDFL IRTKKDGKQI WEAASKTALK KSWKHYEQEM LKNEKVAAKM LEKDATEVGV KAALEEAKKV QIELDTSLSY ITGVKIRDLV KHRNERVCIK GWIHRMRRQG KSLMFFILRD GTGFLQVLLM DKLCQTYDAL TVNTECTVEI YGAIKEVPEG KEAPNGHELI ADFWKIIGNA PPGGIDNVLN EEASVDKMLD NRHLVIRGEN AAALLRLRAA ATRAMREHFY NAGYLEVAPP TLVQTQVEGG STLFNLDYFG EQSFLTQSSQ LYLETCIPTL GDVFCIAQSY RAEKSRTRRH LAEYAHVEAE CPFITLDDLM EKIEELVCDT VDRLLADEEA KKLLEHINPK FQPPERPFLR MEYKDAIKWL QEHNVENEFG NTFTYGEDIA EAAERFMTDT INKPILLNRF PSEIKAFYMQ RDAQDNTLTE SVDLLMPGVG EIVGGSMRIW KFDELSKAFK NVEIDPKPYY WYLDQRLYGT CPHGGYGLGL ERFICWLTNT NHIRDGRCGV CFIFSALGAL EMYVALRTKK RPVKLSVQDV MDCSGMEKCK GRGGNEPAVF RWVAEHGVKT DKSYPYKEND SVSCPRNTPQ RRKYGLADAF YLPPSNEQIL KKILALYGPV CVSLHSSLQS FVAYRSEKVN HAVIAVGYGV QNGMEYFIIK NSWGPTWGQK GYGRIRAGVF MCGIGHFSNV PIFK

NT Sequence

atgactgttt acatttgtcc agaaactgga gatgatggta atgatggtag cgagctgaaa cctttaagga cgctttatca ggcaatgata attactaagt cttccaaagg tgacttctta ataaggacaa agaaagatgg aaaacaaata tgggaagctg cttcaaagac ggcattgaag aaaagttgga aacattacga gcaagaaatg ttgaagaatg aaaaagttgc tgcaaagatg ttagagaagg atgcgacaga agtgggagta aaagcagccc ttgaagaagc caaaaaagtg caaatagaac tggacacgag tctgtcatat atcactggtg tgaagattcg tgatctagtt aagcaccgta atgaacgtgt ttgcatcaaa ggatggattc atagaatgcg tcgacaagga aagtcactga tgtttttcat ccttcgagat gggactggtt tccttcaagt actactcatg gataaattgt gccagacgta tgacgctctc actgtgaata ctgaatgcac ggttgagata tacggcgcaa taaaggaagt tccggaaggt aaagaggcac cgaacgggca cgagttaata gctgattttt ggaaaataat aggaaatgca cctcctggag gcattgataa tgtgttaaac gaagaagcaa gtgtcgacaa aatgttggac aatagacatt tggttattcg aggtgaaaat gctgctgcat tgctacgttt gagagcagct gctactcgag caatgcggga acatttctat aatgctggct atttggaagt tgcaccaccg actttagtgc aaacgcaggt agaaggtgga tcaacgttat ttaatctgga ttattttggt gagcaatctt ttcttacgca gtcatcacag ctctatctag aaacttgtat tcccactctt ggtgatgtct tctgcattgc tcagtcttac agggcggaaa aatctcgcac tcgtcgacac ttggcggaat atgcacatgt tgaagccgag tgtcctttca taacgctgga tgatttgatg gagaaaattg aagaactcgt ttgcgacaca gtggatcgac ttttagctga cgaggaggca aaaaaattat tggaacacat taatccgaaa tttcaaccgc cagaaagacc atttttaaga atggaataca aggatgctat taaatggttg caagaacaca atgttgagaa tgaatttggt aatactttta cttatgggga agatattgct gaagcagcgg aacgtttcat gactgatacc atcaataagc ctattctact gaatcgtttc ccgagcgaaa ttaaggcatt ttatatgcag agggatgcac aggataatac cttaacagaa tctgttgatc ttcttatgcc tggcgttggc gaaattgttg gtggcagtat gagaatttgg aagttcgatg aactatcgaa agcttttaaa aatgtggaaa ttgacccgaa accgtattac tggtacttgg atcaacgttt atatggcacc tgtcctcatg gtggctatgg acttggtttg gaacgtttta tttgttggtt aacaaacacc aatcatattc gcgatggtcg ttgtggtgtg tgttttattt tctcagcatt aggagcgctt gaaatgtatg tggcgctcag gactaaaaaa cggccggtaa aactatcagt acaggatgta atggattgta gtggaatgga aaaatgcaaa ggaagaggcg gaaatgaacc tgcggttttt cgttgggttg ctgagcacgg tgtaaagacc gataagagtt acccatataa ggaaaatgat agtgtttcat gcccgagaaa taccccacaa cgacgaaagt atggtttagc tgatgcattc tatttacctc ctagcaatga acaaattctc aagaagatac tagcactata tggaccagtt tgtgtatcgt tacattcgtc attacaaagt tttgtagctt atcgaagtga aaaagtaaat catgcggtaa tagcggttgg ttatggtgtc cagaatggga tggaatattt tatcatcaaa aacagttggg gccctacatg gggtcaaaaa ggatacggtc gtattcgcgc tggggtattt atgtgcggta ttggtcattt ttcgaacgta ccaatcttca aatga

Details for BrmaA.00825.b.UX2.GE45546

HARVESTED ON:	7/3/2024
SEQUENCED ON:	7/3/2024
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG STGVKIRDLV KHRNERVCIK GWIHRMRRQG KSLMFFILRD GTGFLQVLLM DKLCQTYDAL TVNTECTVEI YGAIKEVPEG KEAPNGHELI ADFWKIIGNA PPGGIDNVLN EEASVDKMLD NRHLVIRGEN AAALLRLRAA ATRAMREHFY NAGYLEVAPP TLVQTQVEGG STLFNLDYFG EQSFLTQSSQ LYLETCIPTL GDVFCIAQSY RAEKSRTRRH LAEYAHVEAE CPFITLDDLM EKIEELVCDT VDRLLADEEA KKLLEHINPK FQPPERPFLR MEYKDAIKWL QEHNVENEFG NTFTYGEDIA EAAERFMTDT INKPILLNRF PSEIKAFYMQ RDAQDNTLTE SVDLLMPGVG EIVGGSMRIW KFDELSKAFK NVEIDPKPYY WYLDQRLYGT CPHGGYGLGL ERFICWLTNT NHIRDGRCG

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga cgggcgttaa aatccgggac ctggtgaaac atcgcaacga gcgggtctgt ataaagggtt ggatacaccg aatgcgccgg caggggaaaa gcttaatgtt ctttattttg cgtgacggta caggattctt gcaggtgtta ctgatggaca agctgtgtca aacatacgat gcgttaacgg tcaacacgga gtgtacagta gaaatttatg gtgccattaa agaggtacct gagggaaagg aagcgccaaa tggacatgaa ttgattgcag acttctggaa gatcatcggc aacgcgccac cgggcgggat cgacaacgtt ttgaatgaag aggccagcgt agataagatg ctcgataacc gccacctcgt catccgtggg gagaacgcag cagctctctt acgcctgcgt gcggcggcaa cccgtgctat gcgcgagcac ttttacaacg caggttacct ggaagtggct ccgccaaccc tggttcagac acaagtcgag ggcgggagca ccttgttcaa cttagactac ttcggggaac agtcgttctt aacccaaagc agtcaactgt acttggagac gtgcatccca accctgggcg acgtgttttg tatagcacaa tcgtatcgtg ccgagaagtc ccgtacccgg cgtcatttag cagagtacgc ccacgtagag gcagaatgcc cgtttatcac attagacgac ctgatggaaa agatagagga gctggtctgt gataccgtag accgcctgct ggcggatgaa gaagctaaga agctgctgga gcatataaac ccaaagttcc agccaccgga gcgtcctttc ttgcggatgg agtataaaga cgcaataaag tggctgcagg agcataacgt agaaaacgag ttcgggaaca ccttcaccta cggcgaggac atagcagagg ctgccgagcg ctttatgacg gacacgataa acaaacccat actgttgaac cgctttccta gtgagatcaa agctttctac atgcaacgtg acgcccaaga caacacgctg actgagtccg tggacttgct gatgccaggt gtaggggaga tcgtgggcgg atcgatgcgc atctggaaat ttgacgagtt gtcaaaggcc ttcaagaacg tcgagatcga tcctaagcca tactattggt atttagacca gcggctgtac ggtacatgcc cgcacggtgg atacgggtta ggcttagagc gattcatctg ctggttgact aatacgaacc acatccgaga cggcagatgc ggctgactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat