BrmaA.00785.c

Cathepsin L-like cysteine proteinase

CENTER ID: BrmaA.00785.c
ORGANISM: Brugia malayi
ASSOCIATED DISEASE: Lymphatic filariasis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrmaA.00785.c.B2.GE44587 mature protein 20 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: Q6E7B3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWTIAQLAVF AFLADFAVAG EIEQLKEVLG KFDKDYKQGN MTRLASDFRS ALKEYGDGQQ GESTVVQEFL KKMESNGEQR AMEKLETEWK DYVTALGKHY DQKETNFRMA IFESNELMTE KINKKYEQGL VSYTTALNDL ADLTDEEFMV RNGLRLPNQT DLRGKRQTSE FYRYDKREQL PDQVDWRTKG AVTPVRNQCG SCYAFATAAA LEAYHKQMTG RLLDLSPQNI VDCTWDLGNK GCNGGYMPTA FEYAARNAIA AESKYPYVET EQRCKWQQSI AVAKDNGFNQ IPPGDELALK HAVAKRGPVV VGISASKRSF RFYKDGVYSE GNCGRPDHAV LVVGYGTHQS YGDYWIVKNS WGTDWGKDGY VYMARNRGNM CDIASMASFP V
NT Sequence
atgtggacaa ttgcgcaatt ggcggtattc gccttcctag ccgattttgc tgtcgctggc gaaatagaac agctgaaaga agttctagga aagtttgaca aagattacaa acagggcaat atgacgagac ttgcgtccga tttcagaagt gcattgaagg aatatggcga tggacaacaa ggggaatcga ccgttgtgca agaattcttg aaaaaaatgg agagcaatgg cgagcaacga gcaatggaaa aattagaaac cgaatggaaa gattatgtaa ccgctctcgg aaaacattac gatcaaaagg aaaccaattt tagaatggca atatttgaaa gcaacgaatt aatgacggaa aagataaata aaaaatatga acaaggctta gtttcttata caacggccct caatgacttg gctgatttga ccgatgaaga atttatggtc aggaatggac ttcgactgcc caatcaaact gatcttcgtg ggaagagaca gacttctgaa ttttataggt atgataaaag ggagcaactg ccagatcaag ttgactggag aacgaaaggc gctgtaacac ccgttagaaa tcagtgcggt agttgctatg cattcgctac agctgcagca ttggaagctt atcataagca gatgactgga agacttcttg atttatcccc tcaaaacatt gttgactgca cgtgggacct cggtaacaag ggttgtaacg gaggctatat gcccacggca ttcgaatacg cagcgagaaa tgcaattgca gcggaatcga aatatccgta tgttgaaact gaacaaagat gcaaatggca acaaagcatc gctgttgcca aggataatgg tttcaaccaa ataccgccgg gtgacgaatt agcacttaag catgccgtag ccaaacgtgg acctgtcgtt gttggaattt ccgcatcgaa acgctcgttc agattttata aagacggtgt ttactctgaa ggaaactgtg gtcgtcctga ccacgcagta ctggtggttg gttacggaac gcatcagtct tatggggatt actggattgt taagaacagt tggggtaccg attggggaaa agatggatat gtttatatgg cacgaaacag agggaatatg tgcgatatcg cctctatggc ttcatttccc gtttaa
Details for BrmaA.00785.c.B2.GE44587
HARVESTED ON: 1/17/2022
SEQUENCED ON: 1/20/2022
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGE IEQLKEVLGK FDKDYKQGNM TRLASDFRSA LKEYGDGQQG ESTVVQEFLK KMESNGEQRA MEKLETEWKD YVTALGKHYD QKETNFRMAI FESNELMTEK INKKYEQGLV SYTTALNDLA DLTDEEFMVR NGLRLPNQTD LRGKRQTSEF YRYDKREQLP DQVDWRTKGA VTPVRNQCGS CYAFATAAAL EAYHKQMTGR LLDLSPQNIV DCTWDLGNKG CNGGYMPTAF EYAARNAIAA ESKYPYVETE QRCKWQQSIA VAKDNGFNQI PPGDELALKH AVAKRGPVVV GISASKRSFR FYKDGVYSEG NCGRPDHAVL VVGYGTHQSY GDYWIVKNSW GTDWGKDGYV YMARNRGNMC DIASMASFPV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcgaaa tagaacagct gaaagaagtt ctaggaaagt ttgacaaaga ttacaaacag ggcaatatga cgagacttgc gtccgatttc agaagtgcat tgaaggaata tggcgatgga caacaagggg aatcgaccgt tgtgcaagaa ttcttgaaaa aaatggagag caatggcgag caacgagcaa tggaaaaatt agaaaccgaa tggaaagatt atgtaaccgc tctcggaaaa cattacgatc aaaaggaaac caattttaga atggcaatat ttgaaagcaa cgaattaatg acggaaaaga taaataaaaa atatgaacaa ggcttagttt cttatacaac ggccctcaat gacttggctg atttgaccga tgaagaattt atggtcagga atggacttcg actgcccaat caaactgatc ttcgtgggaa gagacagact tctgaatttt ataggtatga taaaagggag caactgccag atcaagttga ctggagaacg aaaggcgctg taacacccgt tagaaatcag tgcggtagtt gctatgcatt cgctacagct gcagcattgg aagcttatca taagcagatg actggaagac ttcttgattt atcccctcaa aacattgttg actgcacgtg ggacctcggt aacaagggtt gtaacggagg ctatatgccc acggcattcg aatacgcagc gagaaatgca attgcagcgg aatcgaaata tccgtatgtt gaaactgaac aaagatgcaa atggcaacaa agcatcgctg ttgccaagga taatggtttc aaccaaatac cgccgggtga cgaattagca cttaagcatg ccgtagccaa acgtggacct gtcgttgttg gaatttccgc atcgaaacgc tcgttcagat tttataaaga cggtgtttac tctgaaggaa actgtggtcg tcctgaccac gcagtactgg tggttggtta cggaacgcat cagtcttatg gggattactg gattgttaag aacagttggg gtaccgattg gggaaaagat ggatatgttt atatggcacg aaacagaggg aatatgtgcg atatcgcctc tatggcttca tttcccgttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat