SSGCID: Target Page

BrmaA.00739.a

protein phosphatase 2A., putative

CENTER ID:	BrmaA.00739.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00739.a.B4.GE39471	protein kinase		118	375
BrmaA.00739.a.B5.GE39472	serine threonine phosphatase		475	731

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001899715.1
UniProt:	A8Q2I7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPALETVNK EKNKGVRKRL FSRFLSKLNL ISLQTRAQHL YDRINESITS LAYIRYSETR RRYNSSEKAI DTTVTIFCNQ EIQTRKVGHG SLRESVWPVP LLEAIFLPEF PNNSHITEKD FLILNCIGNG TFSKVYRVCL KLDHNFLFAI KKQSKSEVLM RNVIQQVKDE ASIHRALSDN VFITKCYASW QSRTHLFTLL QYAMGYGDLF TLWRDYGPFM EDTLRIYGAE IAFALDYIHR NNVVYRDLKM ENIVLDLDGH IQIVDFGFSK KLSNGERTRT YMAPEIAKEK FYGKEVDWWS YGVLLHIMNT NSYPFPNCDV SSHTELRYDS YSTPCCDPML GDLFNKLLSV DGERRMNTFA QVKSHPFFES INWDDVALKK LVPFAHTEKL QRCPSYNFLY DKSTSDFSDT EVEENWVAFE EQYEENKLPK ADGKTKSRLS HIHDISIRLL NAGLRKGTHL RRIVKEEELI GLLQETKAVL QSQNTFTEIN TPVVVCGDIH GQYNDMLRIF DKCGFPPESN YLFLGDYVDR GKQSIETVCL LFCFKIKYPE NFFLLRGNHE CAGINRVYGF YEEVNRRYRS VRLWEDTFNY LPYAACIAGK ILCMHGGLSP KLNDFDSLRN IKRPSDPQPT SMELDILWSD PDERVQGWQP NSRGISFVFG VDVLTDVCEK LNIDLVARAH QVVQDGYEFF GNRRLVTIFS APHYCGEFDN AAAAMNVAED LSCSFHVFKP TAKAVRMAMK QKEKAE

NT Sequence

atgacacctg cattagaaac tgtgaataaa gaaaaaaata aaggagtgag aaaaaggctg tttagtcgat ttctaagtaa gctaaactta atatcgctac aaacgagggc ccaacatttg tacgatcgaa ttaatgaaag cattacttca cttgcatata ttcgatattc tgaaacgaga cgacgctata attcaagtga aaaagcgatc gatacaactg tgacaatctt ttgcaatcag gaaattcaaa cgagaaaagt tggtcatggc tcactcaggg aaagtgtttg gccagtgcca ttgctggaag caatttttct accggaattc ccgaataatt cacatatcac agaaaaagat ttcttgatcc tgaattgcat tggaaacggc acattcagta aagtatatag ggtatgcttg aaacttgatc acaacttttt atttgccata aaaaagcagt cgaaaagcga agttttaatg aggaacgtca ttcaacaagt taaagatgaa gcttcaatac atagagctct ctcggataac gttttcatca cgaaatgtta tgctagctgg cagtcgagga cacatctctt cacactactt caatatgcaa tgggttacgg ggatttgttc acgctatggc gtgattatgg acccttcatg gaagatacat taagaatata tggtgccgaa atcgcttttg ctttagatta tattcacaga aacaacgtag tttaccgaga tttaaaaatg gaaaatattg tgcttgatct tgatggccat attcagattg ttgatttcgg tttctccaaa aaactttcca atggagagcg aacacgaacg tacatggctc ccgaaatagc taaggagaaa ttttatggca aagaagtgga ttggtggtct tatggagtat tacttcatat catgaataca aatagttatc catttccaaa ttgtgatgtc agttcgcata ctgaacttag atatgacagt tatagtactc cctgttgtga tccaatgcta ggcgaccttt tcaataagtt attgagtgta gacggagaac gaagaatgaa tacatttgcg caagtgaaat ctcatccttt ttttgaaagc attaattggg atgatgttgc actgaaaaag ttagtaccat ttgcccatac agagaaattg caacgttgtc caagctacaa ttttttgtat gacaagagca catctgattt cagtgacaca gaggtagagg agaactgggt tgcttttgag gaacaatatg aggaaaacaa actgccaaaa gcggatggga aaacgaagtc acgattatca catattcatg acataagcat tcgcctgcta aatgctggat tacggaaggg gacgcacctg agaagaatag taaaggaaga ggaattaata ggattacttc aagaaacaaa agctgtgcta caaagtcaaa atacatttac tgaaattaat acaccagtgg tcgtttgtgg tgatatacat ggacagtaca acgatatgct tcggattttt gacaaatgcg gttttccacc ggaatcgaac tatttattcc ttggtgatta tgtagatcgt ggcaagcagt caattgaaac cgtgtgcctc cttttctgtt tcaaaattaa gtatcctgaa aatttctttt tattaagagg aaatcatgaa tgtgctggaa ttaatcgagt gtatggattc tatgaagagg tcaaccgacg ttatcgttct gttcgtcttt gggaggatac attcaattac ctaccatatg ctgcttgcat agctgggaaa attttgtgta tgcacggcgg tctgtcacca aaattaaatg atttcgattc gttgcgtaac ataaaacgtc catcagatcc acaaccgacc tccatggaac ttgacattct ttggtcagat ccagacgaaa gagtacaagg ttggcaacca aatagccgag gtatctcgtt tgttttcggt gttgatgttt tgacagacgt atgcgagaag ctaaacatcg atctagtcgc tcgtgcacat caggtggtac aagatggata cgagtttttt ggcaatcgtc gtttagtgac catattttcg gcaccacatt actgtggaga gtttgataat gcagctgcag cgatgaatgt agcagaagat ctcagttgct cgtttcacgt gtttaagcca acagcaaaag cagttcgcat ggcaatgaaa caaaaagaaa aagctgaata a

Details for BrmaA.00739.a.B4.GE39471

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	31kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHEK DFLILNCIGN GTFSKVYRVC LKLDHNFLFA IKKQSKSEVL MRNVIQQVKD EASIHRALSD NVFITKCYAS WQSRTHLFTL LQYAMGYGDL FTLWRDYGPF MEDTLRIYGA EIAFALDYIH RNNVVYRDLK MENIVLDLDG HIQIVDFGFS KKLSNGERTR TYMAPEIAKE KFYGKEVDWW SYGVLLHIMN TNSYPFPNCD VSSHTELRYD SYSTPCCDPM LGDLFNKLLS VDGERRMNTF AQVKSHPFFE SINWDD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaaaag atttcttgat cctgaattgc attggaaacg gcacattcag taaagtatat agggtatgct tgaaacttga tcacaacttt ttatttgcca taaaaaagca gtcgaaaagc gaagttttaa tgaggaacgt cattcaacaa gttaaagatg aagcttcaat acatagagct ctctcggata acgttttcat cacgaaatgt tatgctagct ggcagtcgag gacacatctc ttcacactac ttcaatatgc aatgggttac ggggatttgt tcacgctatg gcgtgattat ggacccttca tggaagatac attaagaata tatggtgccg aaatcgcttt tgctttagat tatattcaca gaaacaacgt agtttaccga gatttaaaaa tggaaaatat tgtgcttgat cttgatggcc atattcagat tgttgatttc ggtttctcca aaaaactttc caatggagag cgaacacgaa cgtacatggc tcccgaaata gctaaggaga aattttatgg caaagaagtg gattggtggt cttatggagt attacttcat atcatgaata caaatagtta tccatttcca aattgtgatg tcagttcgca tactgaactt agatatgaca gttatagtac tccctgttgt gatccaatgc taggcgacct tttcaataag ttattgagtg tagacggaga acgaagaatg aatacatttg cgcaagtgaa atctcatcct ttttttgaaa gcattaattg ggatgattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BrmaA.00739.a.B5.GE39472

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	30kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHME TKAVLQSQNT FTEINTPVVV CGDIHGQYND MLRIFDKCGF PPESNYLFLG DYVDRGKQSI ETVCLLFCFK IKYPENFFLL RGNHECAGIN RVYGFYEEVN RRYRSVRLWE VFQDTFNYLP YAACIAGKIL CMHGGLSPKL NDFDSLRNIK RPSDPQPTSM ELDILWSDPD ERVQGWQPNS RGISFVFGVD VLTDVCEKLN IDLVARAHQV VQDGYEFFGN RRLVTIFSAP HYCGEFDNAA AAMNVAEDLS CXXHVFKPT

Validated NT Sequence

atcttactca cttatgttgg cttaaacacg tgnnnnnagc aactgagatc ttctgctaca ttcatcgctg cagctgcatt atcaaactct ccacagtaat gtggtgccga aaatatggtc actaaacgac gattgccaaa aaactcgtat ccatcttgta ccacctgatg tgcacgagcg actagatcga tgtttagctt ctcgcatacg tctgtcaaaa catcaacacc gaaaacaaac gagatacctc ggctatttgg ttgccaacct tgtactcttt cgtctggatc tgaccaaaga atgtcaagtt ccatggaggt cggttgtgga tctgatggac gttttatgtt acgcaacgaa tcgaaatcat ttaattttgg tgacagaccg ccgtgcatac acaaaatttt cccagctatg caagcagcat atggtaggta attgaatgta tcttgaaaga cctcccaaag acgaacagaa cgataacgtc ggttgacctc ttcatagaat ccatacactc gattaattcc agcacattca tgatttcctc ttaataaaaa gaaattttca ggatacttaa ttttgaaaca gaaaaggagg cacacggttt caattgactg cttgccacga tctacataat caccaaggaa taaatagttc gattccggtg gaaaaccgca tttgtcaaaa atccgaagca tatcgttgta ctgtccatgt atatcaccac aaacgaccac tggtgtatta atttcagtaa atgtattttg actttgtagc acagcttttg tttccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHET KAVLQSQNTF TEINTPVVVC GDIHGQYNDM LRIFDKCGFP PESNYLFLGD YVDRGKQSIE TVCLLFCFKI KYPENFFLLR GNHECAGINR VYGFYEEVNR RYRSVRLWED TFNYLPYAAC IAGKILCMHG GLSPKLNDFD SLRNIKRPSD PQPTSMELDI LWSDPDERVQ GWQPNSRGIS FVFGVDVLTD VCEKLNIDLV ARAHQVVQDG YEFFGNRRLV TIFSAPHYCG EFDNAAAAMN VAEDLSCSFH VFKPT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaaacaa aagctgtgct acaaagtcaa aatacattta ctgaaattaa tacaccagtg gtcgtttgtg gtgatataca tggacagtac aacgatatgc ttcggatttt tgacaaatgc ggttttccac cggaatcgaa ctatttattc cttggtgatt atgtagatcg tggcaagcag tcaattgaaa ccgtgtgcct ccttttctgt ttcaaaatta agtatcctga aaatttcttt ttattaagag gaaatcatga atgtgctgga attaatcgag tgtatggatt ctatgaagag gtcaaccgac gttatcgttc tgttcgtctt tgggaggata cattcaatta cctaccatat gctgcttgca tagctgggaa aattttgtgt atgcacggcg gtctgtcacc aaaattaaat gatttcgatt cgttgcgtaa cataaaacgt ccatcagatc cacaaccgac ctccatggaa cttgacattc tttggtcaga tccagacgaa agagtacaag gttggcaacc aaatagccga ggtatctcgt ttgttttcgg tgttgatgtt ttgacagacg tatgcgagaa gctaaacatc gatctagtcg ctcgtgcaca tcaggtggta caagatggat acgagttttt tggcaatcgt cgtttagtga ccatattttc ggcaccacat tactgtggag agtttgataa tgcagctgca gcgatgaatg tagcagaaga tctcagttgc tcgtttcacg tgtttaagcc aacatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat