SSGCID: Target Page

BrmaA.00379.a

phosphopyruvate hydratase

CENTER ID:	BrmaA.00379.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00379.a.B1.GE36290	full length		1	436

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00379.a.B1.PW36940	full length		1	436

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001896281.1
UniProt:	A8PFE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPITRVHARP IYDSRGNPTV EVDLTTDKGI FRAAVPSGAS TGVHEALELR DNDKAVNHGK GVLKAVRNVN EHIGPALVAK NFCPTQQREI DHFMLELDGT ENKAKLGANA ILGVSLAVCK AGAVHKGMPL YKYIAELAGT KQIVLPVPAM NVINGGSHAG NKLAMQEFMI MPIGASSFSE AMRMGSEIYH YLKAEIKKRY GLDATAVGDE GGFAPNIQDN REGLDLLNTA IATAGYTGKV AIAMDCAASE YYMESAKLYD LDFKNPTSDK AQWKTGDQMM EIYQSFIKEY PVVSIEDWFD QDDWENWTKA LANTHIQIVG DDLTVTNPKR IAMAAEKKAC NCLLLKVNQI GSVTESIDAA NLARKNGWGV MVSHRSGETE DTFIADLVVG LATGQIKTGA PCRSERLAKY NQILRIEEEL GSAAIYAGQK FRNPQA

NT Sequence

atgccgatca cacgtgttca cgcccgtcct atttatgatt cacgtggtaa tccaaccgtc gaagttgatt tgaccaccga caaaggtatt ttccgtgcgg ctgtaccaag tggtgcttca actggtgtac atgaagcact tgaacttcgg gacaatgata aagctgtgaa tcatggcaaa ggtgttttga aagctgtaag aaatgtcaac gaacatattg gacctgctct agttgctaag aatttttgtc caactcaaca acgtgaaatc gaccatttta tgctrgaact cgatggaacc gaaaataaag caaaactggg tgccaatgca attttgggtg tttcattggc ggtttgcaag gctggtgcag tgcataaagg tatgccgttg tataagtata tagcagaatt ggctggtacc aaacagattg ttctgccagt tcctgctatg aatgttatca acggtggttc tcatgctggt aataaactgg caatgcagga atttatgatc atgcctattg gagctagttc attcagtgaa gcaatgcgca tgggttctga aatttaccat tacttgaagg cagaaatcaa aaaacgatac ggtctcgatg caacagcagt gggtgatgaa ggtggtttcg ctcctaatat tcaggataac agggaaggtc ttgatttgtt gaatacagca attgcaacag ctggatacac gggaaaagta gcaattgcta tggattgtgc cgcatcagaa tattatatgg aatcagctaa gctgtacgat ttagacttca aaaatccaac ctcggataaa gcccagtgga aaactggtga tcaaatgatg gaaatctatc aatccttcat taaggaatat ccagttgtat cgattgagga ttggtttgac caggatgact gggaaaattg gaccaaagca ttggctaata cgcatattca aattgttggc gatgacttaa ctgttacgaa tcctaagaga attgctatgg ctgctgagaa gaaagcttgc aactgcctgt tactcaaggt taatcaaatt ggctcagtga ctgaatcaat tgatgcggct aacttagcac gtaaaaatgg atggggtgta atggtatcgc atcgttcagg tgaaacggaa gatacattta tcgctgatct cgtcgttgga cttgctaccg gacagatcaa aactggagca ccatgtcgtt cggagcgtct cgccaaatac aatcagatac ttcgtattga agaagaactt ggatcagctg ccatttacgc tggtcaaaag ttccgaaatc ctcaagcata g

Details for BrmaA.00379.a.B1.GE36290

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	75

Validated AA Sequence

MLELDGTENK AKLGANAILG VSLAVCKAGA VHKGMPLYKY IAELAGTKQI VLPVPAMNVI NGGSHAGNKL AMQEFMIMPI GASSFSEAMR MGSEIYHYLK AEIKKRYGLD ATAVGDEGGF APNIQDNREG LDLLNTAIAT AGYTGKVAIA MDCAASEYYM ESAKLYDLDF KNPTSDKAQW KTGDQMMEIY QSFIKEYPVV SIEDWFDQDD WENWTKALAN THIQIVGDDL TVTNPKRIAM AAEKKACNCL LLKVNQIGSV TESIDAANLA RKNGWGVMVS HRSGETEDTF IADLVVGLAT GQIKTGAPCR SERLAKYNQI LRIEEELGSA AIYA

Validated NT Sequence

accagcgtaa atggcagctg atccaagttc ttcttcaata cgaagtatct gattgtattt ggcgagacgc tccgaacgac atggtgctcc agttttgatc tgtccggtag caagtccaac gacgagatca gcgataaatg tatcttccgt ttcacctgaa cgatgcgata ccattacacc ccatccattt ttacgtgcta agttagccgc atcaattgat tcagtcactg agccaatttg attaaccttg agtaacaggc agttgcaagc tttcttctca gcagccatag caattctctt aggattcgta acagttaagt catcgccaac aatttgaata tgcgtattag ccaatgcttt ggtccaattt tcccagtcat cctggtcaaa ccaatcctca atcgatacaa ctggatattc cttaatgaag gattgataga tttccatcat ttgatcacca gttttccact gggctttatc cgaggttgga tttttgaagt ctaaatcgta cagcttagct gattccatat aatattctga tgcggcacaa tccatagcaa ttgctacttt tcccgtgtat ccagctgttg caattgctgt attcaacaaa tcaagacctt ccctgttatc ctgaatatta ggagcgaaac caccttcatc acccactgct gttgcatcga gaccgtatcg ttttttgatt tctgccttca agtaatggta aatttcagaa cccatgcgca ttgcttcact gaatgaacta gctccaatag gcatgatcat aaattcctgc attgccagtt tattaccagc atgagaacca ccgttgataa cattcatagc aggaactggc agaacaatct gtttggtacc agccaattct gctatatact tatacaacgg cataccttta tgcactgcac cagccttgca aaccgccaat gaaacaccca aaattgcatt ggcacccagt tttgctttat tttcggttcc atcgagttcc agcataaaat ggtcgatttc acgttgttga gttggacaaa aattcttagc aactagagca ggtccaatat gttcgttgac atttcttaca gctttcaaaa cacctttgcc atgattcaca gctttatcat tgtcccgaag ttcaagtgct tcatgtacac cagttgaagc accacttggt acagccgcac ggaaaatacc tttgtcggtg gtcaaatcaa cttcgacggt tggattacca cgtgaatcat aaataggacg ggcgtgaaca cgtgtga

Expected Protein Sequence

MAHHHHHHMP ITRVHARPIY DSRGNPTVEV DLTTDKGIFR AAVPSGASTG VHEALELRDN DKAVNHGKGV LKAVRNVNEH IGPALVAKNF CPTQQREIDH FMELDGTENK AKLGANAILG VSLAVCKAGA VHKGMPLYKY IAELAGTKQI VLPVPAMNVI NGGSHAGNKL AMQEFMIMPI GASSFSEAMR MGSEIYHYLK AEIKKRYGLD ATAVGDEGGF APNIQDNREG LDLLNTAIAT AGYTGKVAIA MDCAASEYYM ESAKLYDLDF KNPTSDKAQW KTGDQMMEIY QSFIKEYPVV SIEDWFDQDD WENWTKALAN THIQIVGDDL TVTNPKRIAM AAEKKACNCL LLKVNQIGSV TESIDAANLA RKNGWGVMVS HRSGETEDTF IADLVVGLAT GQIKTGAPCR SERLAKYNQI LRIEEELGSA AIYAGQKFRN PQA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccga tcacacgtgt tcacgcccgt cctatttatg attcacgtgg taatccaacc gtcgaagttg atttgaccac cgacaaaggt attttccgtg cggctgtacc aagtggtgct tcaactggtg tacatgaagc acttgaactt cgggacaatg ataaagctgt gaatcatggc aaaggtgttt tgaaagctgt aagaaatgtc aacgaacata ttggacctgc tctagttgct aagaattttt gtccaactca acaacgtgaa atcgaccatt ttatgctrga actcgatgga accgaaaata aagcaaaact gggtgccaat gcaattttgg gtgtttcatt ggcggtttgc aaggctggtg cagtgcataa aggtatgccg ttgtataagt atatagcaga attggctggt accaaacaga ttgttctgcc agttcctgct atgaatgtta tcaacggtgg ttctcatgct ggtaataaac tggcaatgca ggaatttatg atcatgccta ttggagctag ttcattcagt gaagcaatgc gcatgggttc tgaaatttac cattacttga aggcagaaat caaaaaacga tacggtctcg atgcaacagc agtgggtgat gaaggtggtt tcgctcctaa tattcaggat aacagggaag gtcttgattt gttgaataca gcaattgcaa cagctggata cacgggaaaa gtagcaattg ctatggattg tgccgcatca gaatattata tggaatcagc taagctgtac gatttagact tcaaaaatcc aacctcggat aaagcccagt ggaaaactgg tgatcaaatg atggaaatct atcaatcctt cattaaggaa tatccagttg tatcgattga ggattggttt gaccaggatg actgggaaaa ttggaccaaa gcattggcta atacgcatat tcaaattgtt ggcgatgact taactgttac gaatcctaag agaattgcta tggctgctga gaagaaagct tgcaactgcc tgttactcaa ggttaatcaa attggctcag tgactgaatc aattgatgcg gctaacttag cacgtaaaaa tggatggggt gtaatggtat cgcatcgttc aggtgaaacg gaagatacat ttatcgctga tctcgtcgtt ggacttgcta ccggacagat caaaactgga gcaccatgtc gttcggagcg tctcgccaaa tacaatcaga tacttcgtat tgaagaagaa cttggatcag ctgccattta cgctggtcaa aagttccgaa atcctcaagc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BrmaA.00379.a.B1.PW36940

PURIFICATION DATe:	12/31/2012
CONCENTRATION:	34.5mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	20
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	75

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGA TCACACGTGT TCACGCCCGT CCTATTTATG ATTCACGTGG TAATCCAACC GTCGAAGTTG ATTTGACCAC CGACAAAGGT ATTTTCCGTG CGGCTGTACC AAGTGGTGCT TCAACTGGTG TACATGAAGC ACTTGAACTT CGGGACAATG ATAAAGCTGT GAATCATGGC AAAGGTGTTT TGAAAGCTGT AAGAAATGTC AACGAACATA TTGGACCTGC TCTAGTTGCT AAGAATTTTT GTCCAACTCA ACAACGTGAA ATCGACCATT TTATGCTRGA ACTCGATGGA ACCGAAAATA AAGCAAAACT GGGTGCCAAT GCAATTTTGG GTGTTTCATT GGCGGTTTGC AAGGCTGGTG CAGTGCATAA AGGTATGCCG TTGTATAAGT ATATAGCAGA ATTGGCTGGT ACCAAACAGA TTGTTCTGCC AGTTCCTGCT ATGAATGTTA TCAACGGTGG TTCTCATGCT GGTAATAAAC TGGCAATGCA GGAATTTATG ATCATGCCTA TTGGAGCTAG TTCATTCAGT GAAGCAATGC GCATGGGTTC TGAAATTTAC CATTACTTGA AGGCAGAAAT CAAAAAACGA TACGGTCTCG ATGCAACAGC AGTGGGTGAT GAAGGTGGTT TCGCTCCTAA TATTCAGGAT AACAGGGAAG GTCTTGATTT GTTGAATACA GCAATTGCAA CAGCTGGATA CACGGGAAAA GTAGCAATTG CTATGGATTG TGCCGCATCA GAATATTATA TGGAATCAGC TAAGCTGTAC GATTTAGACT TCAAAAATCC AACCTCGGAT AAAGCCCAGT GGAAAACTGG TGATCAAATG ATGGAAATCT ATCAATCCTT CATTAAGGAA TATCCAGTTG TATCGATTGA GGATTGGTTT GACCAGGATG ACTGGGAAAA TTGGACCAAA GCATTGGCTA ATACGCATAT TCAAATTGTT GGCGATGACT TAACTGTTAC GAATCCTAAG AGAATTGCTA TGGCTGCTGA GAAGAAAGCT TGCAACTGCC TGTTACTCAA GGTTAATCAA ATTGGCTCAG TGACTGAATC AATTGATGCG GCTAACTTAG CACGTAAAAA TGGATGGGGT GTAATGGTAT CGCATCGTTC AGGTGAAACG GAAGATACAT TTATCGCTGA TCTCGTCGTT GGACTTGCTA CCGGACAGAT CAAAACTGGA GCACCATGTC GTTCGGAGCG TCTCGCCAAA TACAATCAGA TACTTCGTAT TGAAGAAGAA CTTGGATCAG CTGCCATTTA CGCTGGTCAA AAGTTCCGAA ATCCTCAAGC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT