SSGCID: Target Page

BrmaA.00186.b

Cathepsin L-like, putative

CENTER ID:	BrmaA.00186.b
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00186.b.B2.GE44585	mature protein		20	393

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A4E9FMQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MWTIAQLAVF AILADFAVAG EIEQLKEVLG KFDKDYKQGN MTRLASDFRS ALKEYGDGQQ GESTVVQEFL KKMEDNGEQR AMEKLESEWK DYVTALGKHY DQKETNFRMA IFESNELMTE KINKKYEQGL VSYTTALNDL ADLTDEEFMV RNGLRLPNQT DLRGKRQTSE FYRYDKREQL PDQVDWRTKG AVTPVRNQGQ CGSCYAFATA AALEAYHKQM TGRLLDLSPQ NIVDCTWDLG NKGCNGGYMP TAFEYAARNA IAAESKYPYV ETEQRCKWQQ SIAVAKDNGF NQIPPGDELA LKHAVAKRGP VVVGISASKR SFRFYKDGVY SEGNCGRPDH AVLVVGYGTH QSYGDYWIVK NSWGTDWGKD GYVYMARNRG NMCDIASMAS FPV

NT Sequence

atgtggacaa ttgcgcaatt ggcggtattc gccatcctag ccgattttgc tgtcgctggc gaaatagaac agctgaaaga agttctagga aagtttgaca aagattacaa acagggcaat atgacgagac ttgcgtccga tttcagaagt gcattgaagg aatatggcga tggacaacaa ggggaatcga ccgttgtgca agaattcttg aaaaaaatgg aggacaatgg cgagcaacga gcaatggaaa aattagaaag cgaatggaaa gattatgtaa ccgctctcgg aaaacattac gatcaaaagg aaaccaattt tagaatggca atatttgaaa gcaacgaatt aatgacggaa aagataaata aaaaatatga acaaggctta gtttcttata caacggccct caatgacttg gctgatttga ccgatgaaga atttatggtc aggaatggac ttcgactgcc caatcaaact gatcttcgtg ggaagagaca gacttctgaa ttttataggt atgataaaag ggagcaactg ccagatcaag ttgactggag aacgaaaggc gctgtaacac ccgttagaaa tcagggacag tgcggtagtt gctatgcatt cgctacagct gcagcattgg aagcttatca taagcagatg actggaagac ttcttgattt atcccctcaa aacattgttg actgcacgtg ggacctcggt aacaagggtt gtaacggagg ctatatgccc acggcattcg aatacgcagc gagaaatgca attgcagcgg aatcgaaata tccgtatgtt gaaactgaac aaagatgcaa atggcaacaa agcatcgctg ttgccaagga taatggtttc aaccaaatac cgccgggtga cgaattagca cttaagcatg ccgtagccaa acgtggacct gtcgttgttg gaatttccgc atcgaaacgc tcgttcagat tttataaaga cggtgtttac tctgaaggaa actgtggtcg tcctgaccac gcagtactgg tggttggtta cggaacgcat cagtcttatg gggattactg gattgttaag aacagttggg gtaccgattg gggaaaagat ggatatgttt atatggcacg aaacagaggg aatatgtgcg atatcgcctc tatggcttca tttcccgttt aa

Details for BrmaA.00186.b.B2.GE44585

HARVESTED ON:	1/17/2022
SEQUENCED ON:	1/20/2022
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGE IEQLKEVLGK FDKDYKQGNM TRLASDFRSA LKEYGDGQQG ESTVVQEFLK KMEDNGEQRA MEKLESEWKD YVTALGKHYD QKETNFRMAI FESNELMTEK INKKYEQGLV SYTTALNDLA DLTDEEFMVR NGLRLPNQTD LRGKRQTSEF YRYDKREQLP DQVDWRTKGA VTPVRNQGQC GSCYAFATAA ALEAYHKQMT GRLLDLSPQN IVDCTWDLGN KGCNGGYMPT AFEYAARNAI AAESKYPYVE TEQRCKWQQS IAVAKDNGFN QIPPGDELAL KHAVAKRGPV VVGISASKRS FRFYKDGVYS EGNCGRPDHA VLVVGYGTHQ SYGDYWIVKN SWGTDWGKDG YVYMARNRGN MCDIASMASF PV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcgaaa tagaacagct gaaagaagtt ctaggaaagt ttgacaaaga ttacaaacag ggcaatatga cgagacttgc gtccgatttc agaagtgcat tgaaggaata tggcgatgga caacaagggg aatcgaccgt tgtgcaagaa ttcttgaaaa aaatggagga caatggcgag caacgagcaa tggaaaaatt agaaagcgaa tggaaagatt atgtaaccgc tctcggaaaa cattacgatc aaaaggaaac caattttaga atggcaatat ttgaaagcaa cgaattaatg acggaaaaga taaataaaaa atatgaacaa ggcttagttt cttatacaac ggccctcaat gacttggctg atttgaccga tgaagaattt atggtcagga atggacttcg actgcccaat caaactgatc ttcgtgggaa gagacagact tctgaatttt ataggtatga taaaagggag caactgccag atcaagttga ctggagaacg aaaggcgctg taacacccgt tagaaatcag ggacagtgcg gtagttgcta tgcattcgct acagctgcag cattggaagc ttatcataag cagatgactg gaagacttct tgatttatcc cctcaaaaca ttgttgactg cacgtgggac ctcggtaaca agggttgtaa cggaggctat atgcccacgg cattcgaata cgcagcgaga aatgcaattg cagcggaatc gaaatatccg tatgttgaaa ctgaacaaag atgcaaatgg caacaaagca tcgctgttgc caaggataat ggtttcaacc aaataccgcc gggtgacgaa ttagcactta agcatgccgt agccaaacgt ggacctgtcg ttgttggaat ttccgcatcg aaacgctcgt tcagatttta taaagacggt gtttactctg aaggaaactg tggtcgtcct gaccacgcag tactggtggt tggttacgga acgcatcagt cttatgggga ttactggatt gttaagaaca gttggggtac cgattgggga aaagatggat atgtttatat ggcacgaaac agagggaata tgtgcgatat cgcctctatg gcttcatttc ccgtttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat