SSGCID: Target Page

BrmaA.00164.a

BMA-RARS-2; likely arginyl-tRNA synthetase family protein (ArgRS); uncharacterized protein

CENTER ID:	BrmaA.00164.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00164.a.B1.GE41312	full length		1	500

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H5S9T5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLHVADSSMK RFVYEMWREE NLPATLLQKL VKNRKSIVVD YSSPNIAKQF HVGNFRSTVI GRYIDTINRA AGNKVISLNY IGDWGLQFAL IAAHWPVMKP SQEVWSTWNT VQKFTFLAKC YVEANKLAEH SSSFLQKAYN ILASMENSLL TAGTNDHLSF WQNARQLSHE QLHHFYRRMN LSMDVEYFES DFVVSAHQLI NRMLREGQAE NGSDGVVTVI CKELNRSIVI RRSNNTTLYL SRDLASVLLR EQLYAADKYF YVVDHAQRQH FTNLKCLLNA IGRNDIGEKI HHVSFGRVKG LSTRLGEVEL VSKILEHGLE LAKSFITKSR TMKISQNEIP QVAANLSLGT MIVNDLKRAR STNYSFSFDK AFQMNQNNAL LLQTKHSRLM SIEERNHGLK QNLMEGFEGN FETNEASWLL LKQFKAFPDI LYASYQEMEP CRLTVYLLQL ANVVGFASSI LRISNEPLEI AIPRLLLLST ARKILDSGMR LLGIEPLKKM

NT Sequence

atgctgcatg tagctgactc atcgatgaaa agatttgtgt atgagatgtg gcgggaggaa aatctgcctg caacgttatt gcagaaattg gtcaagaata ggaaatctat tgttgttgat tactcttccc caaatattgc caaacaattt catgttggca atttcagatc cactgttatt gggcgctata tcgatacaat aaatagagct gctggcaata aagttatatc tctcaactac atcggtgatt gggggttgca gtttgcactg attgctgctc actggcctgt aatgaaaccc tcccaagagg tatggtctac gtggaacact gtgcaaaaat ttacattttt ggctaaatgc tacgttgaag cgaacaaatt agctgaacat tccagcagtt ttttacaaaa agcttacaat attcttgcca gcatggaaaa ttctttgtta accgctggta ccaatgatca tttatcgttc tggcaaaatg caaggcagtt atctcatgaa cagttgcatc atttttatag acgtatgaac ttatcaatgg atgtggaata tttcgagtct gactttgtcg tttctgctca tcagttaatt aatcgtatgt tacgagaagg gcaagcagaa aacggcagtg atggtgttgt tacagtcata tgcaaagaac tgaataggtc tattgttatc agaagatcta ataacacgac tctttatttg tcgagggact tagcatcggt tttattacgg gaacagttgt atgcagcgga taagtatttc tatgtggttg atcatgcaca gcgacaacat ttcaccaatt tgaagtgtct tttgaatgcc attggacgaa atgatattgg tgaaaaaata catcatgtct catttggacg cgtaaaaggt ctttctacca ggctgggaga agttgaattg gtaagtaaaa tactagaaca tggtttggaa cttgcaaaat cctttatcac caaatctaga acgatgaaaa ttagccagaa tgaaattcct caagtcgcag cgaatctgtc acttggaaca atgatagtga atgatctcaa aagggcacga tccaccaatt actcgttttc attcgataag gcttttcaaa tgaatcaaaa taatgcgctt cttctacaga cgaaacatag ccggctaatg tcaatcgagg aacggaatca tggcttgaag cagaatctaa tggagggatt tgaggggaat ttcgaaacga atgaagcgag ttggcttctt ttgaaacaat tcaaagcatt tcctgatatc ctatatgcta gctaccaaga aatggaacct tgccgattaa ccgtgtattt attgcaactg gcaaatgttg taggcttcgc ttcttccatc cttcgaatat caaacgaacc actggagata gcaataccaa gacttttgct attatcaact gcacggaaaa ttctagacag tgggatgcgt ctgctaggaa tagaaccact gaagaaaatg tga

Details for BrmaA.00164.a.B1.GE41312

HARVESTED ON:	3/31/2017
SEQUENCED ON:	4/10/2017
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML HVADSSMKRF VYEMWREENL PATLLQKLVK NRKSIVVDYS SPNIAKQFHV GNFRSTVIGR YIDTINRAAG NKVISLNYIG DWGLQFALIA AHWPVMKPSQ EVWSTWNTVQ KFTFLAKCYV EANKLAEHSS SFLQKAYNIL ASMENSLLTA GTNDHLSFWQ NARQLSHEQL HHFYRRMNLS MDVEYFESDF VVSAHQLINR MLREGQAENG SDGVVTVICK ELNRSIVIRR SNNTTLYLSR DLASVLLREQ LYAADKYFYV VDHAQRQHFT NLKCLLNAIG RNDIGEKIHH VSFGRVKGLS TRLGEVELVS KILEHGLELA KSFITKSRTM KISQNEIPQV AANLSLGTMI VNDLKRARST NYSFSFDKAF QMNQNNALLL QTKHSRLMSI EERNHGLKQN LMEGFEGNFE TNEASWLLLK QFKAFPDILY ASYQEMEPCR LTVYLLQLAN VVGFASSILR ISNEPLEIAI PRLLLLSTAR KILDSGMRLL

Validated NT Sequence

tcctagcaga cgcatcccac tgtctagaat tttccgtgca gttgataata gcaaaagtct tggtattgct atctccagtg gttcgtttga tattcgaagg atggaagaag cgaagcctac aacatttgcc agttgcaata aatacacggt taatcggcaa ggttccattt cttggtagct agcatatagg atatcaggaa atgctttgaa ttgtttcaaa agaagccaac tcgcttcatt cgtttcgaaa ttcccctcaa atccctccat tagattctgc ttcaagccat gattccgttc ctcgattgac attagccggc tatgtttcgt ctgtagaaga agcgcattat tttgattcat ttgaaaagcc ttatcgaatg aaaacgagta attggtggat cgtgcccttt tgagatcatt cactatcatt gttccaagtg acagattcgc tgcgacttga ggaatttcat tctggctaat tttcatcgtt ctagatttgg tgataaagga ttttgcaagt tccaaaccat gttctagtat tttacttacc aattcaactt ctcccagcct ggtagaaaga ccttttacgc gtccaaatga gacatgatgt attttttcac caatatcatt tcgtccaatg gcattcaaaa gacacttcaa attggtgaaa tgttgtcgct gtgcatgatc aaccacatag aaatacttat ccgctgcata caactgttcc cgtaataaaa ccgatgctaa gtccctcgac aaataaagag tcgtgttatt agatcttctg ataacaatag acctattcag ttctttgcat atgactgtaa caacaccatc actgccgttt tctgcttgcc cttctcgtaa catacgatta attaactgat gagcagaaac gacaaagtca gactcgaaat attccacatc cattgataag ttcatacgtc tataaaaatg atgcaactgt tcatgagata actgccttgc attttgccag aacgataaat gatcattggt accagcggtt aacaaagaat tttccatgct ggcaagaata ttgtaagctt tttgtaaaaa actgctggaa tgttcagcta atttgttcgc ttcaacgtag catttagcca aaaatgtaaa tttttgcaca gtgttccacg tagaccatac ctcttgggag ggtttcatta caggccagtg agcagcaatc agtgcaaact gcaaccccca atcaccgatg tagttgagag atataacttt attgccagca gctctattta ttgtatcgat atagcgccca ataacagtgg atctgaaatt gccaacatga aattgtttgg caatatttgg ggaagagtaa tcaacaacaa tagatttcct attcttgacc aatttctgca ataacgttgc aggcagattt tcctcccgcc acatctcata cacaaatctt ttcatcgatg agtcagctac atgcagcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc atgtagctga ctcatcgatg aaaagatttg tgtatgagat gtggcgggag gaaaatctgc ctgcaacgtt attgcagaaa ttggtcaaga ataggaaatc tattgttgtt gattactctt ccccaaatat tgccaaacaa tttcatgttg gcaatttcag atccactgtt attgggcgct atatcgatac aataaataga gctgctggca ataaagttat atctctcaac tacatcggtg attgggggtt gcagtttgca ctgattgctg ctcactggcc tgtaatgaaa ccctcccaag aggtatggtc tacgtggaac actgtgcaaa aatttacatt tttggctaaa tgctacgttg aagcgaacaa attagctgaa cattccagca gttttttaca aaaagcttac aatattcttg ccagcatgga aaattctttg ttaaccgctg gtaccaatga tcatttatcg ttctggcaaa atgcaaggca gttatctcat gaacagttgc atcattttta tagacgtatg aacttatcaa tggatgtgga atatttcgag tctgactttg tcgtttctgc tcatcagtta attaatcgta tgttacgaga agggcaagca gaaaacggca gtgatggtgt tgttacagtc atatgcaaag aactgaatag gtctattgtt atcagaagat ctaataacac gactctttat ttgtcgaggg acttagcatc ggttttatta cgggaacagt tgtatgcagc ggataagtat ttctatgtgg ttgatcatgc acagcgacaa catttcacca atttgaagtg tcttttgaat gccattggac gaaatgatat tggtgaaaaa atacatcatg tctcatttgg acgcgtaaaa ggtctttcta ccaggctggg agaagttgaa ttggtaagta aaatactaga acatggtttg gaacttgcaa aatcctttat caccaaatct agaacgatga aaattagcca gaatgaaatt cctcaagtcg cagcgaatct gtcacttgga acaatgatag tgaatgatct caaaagggca cgatccacca attactcgtt ttcattcgat aaggcttttc aaatgaatca aaataatgcg cttcttctac agacgaaaca tagccggcta atgtcaatcg aggaacggaa tcatggcttg aagcagaatc taatggaggg atttgagggg aatttcgaaa cgaatgaagc gagttggctt cttttgaaac aattcaaagc atttcctgat atcctatatg ctagctacca agaaatggaa ccttgccgat taaccgtgta tttattgcaa ctggcaaatg ttgtaggctt cgcttcttcc atccttcgaa tatcaaacga accactggag atagcaatac caagactttt gctattatca actgcacgga aaattctaga cagtgggatg cgtctgctag gaatagaacc actgaagaaa atgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat