SSGCID: Target Page

BrmaA.00037.a

NADH-ubiquinone oxidoreductase 1, chain, putative

CENTER ID:	BrmaA.00037.a
ORGANISM:	Brugia malayi
ASSOCIATED DISEASE:	Lymphatic filariasis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrmaA.00037.a.B1.GE36286	full length		1	206

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	XP_001895608.1
UniProt:	A8PAV3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSWFCLAYH SGNILSKLPF PANCSQIRTT YKYVAESAKS DGTLLGDFRK AMHYMFMTEL IRGMGIIVGH YFMEPATINY PFEKGPLSSR FRGEHALRRY PSGEERCIAC KLCEAICPAQ AITIEAEARP DGSRRTTRYD IDMTKCIYCG LCQEACPVDA IVEGPNFEYS TETHEELLYN KEKLLLNGDR WEPELAANLQ AEYLYR

NT Sequence

atgcgttcat ggttttgctt ggcatatcac tctgggaaca ttctcagtaa acttccattt ccagcaaact gttcgcaaat tcgcacaaca tacaagtatg ttgctgaatc agcgaaatcc gacggaacac tgctgggtga tttccgcaaa gcaatgcatt atatgttcat gacagagctt ataagaggta tgggtataat tgttggtcat tactttatgg agcctgcaac aataaattat ccattcgaga aaggtccttt gagctcgcgt tttcgtggtg agcatgcact tcggaggtat ccatccggtg aagaacgctg catagcctgc aaactatgcg aagcaatatg tccggcacaa gcaataacga ttgaggctga agcaaggccc gatggaagtc ggcgaactac tcgttatgat attgacatga caaaatgtat ttattgtggt ttatgtcagg aggcttgtcc agttgatgcc attgttgagg gtccgaattt tgaatactca acggagactc atgargagct gctatataac aaagaaaaac ttttgttgaa tggtgatcga tgggagccag aattagctgc aaacttacaa gctgaatatc tttatcgtta g

Details for BrmaA.00037.a.B1.GE36286

HARVESTED ON:	10/23/2012
SEQUENCED ON:	10/26/2012
EXPECTED MW:	24kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	91
PERCENT COVERAGE:	60

Validated AA Sequence

MAHHHHHHMR SWFCLAYHSG NILSKLPFPA NCSQIRTTYK YVAESAKSDG TLLGDFRKAM HYMFMTELIR GMGIIVGHYF MEPATINYPF EKGPLSSRFR GEHALRRYPS GEERCIPANY AKQYVRHKX

Validated NT Sequence

tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgcgttc atggttttgc ttggcatatc actctgggaa cattctcagt aaacttccat ttccagcaaa ctgttcgcaa attcgcacaa catacaagta tgttgctgaa tcagcgaaat ccgacggaac actgctgggt gatttccgca aagcaatgca ttatatgttc atgacagagc ttataagagg tatgggtata attgttggtc attactttat ggagcctgca acaataaatt atccattcga gaaaggtcct ttgagctcgc gttttcgtgg tgagcatgca cttcggaggt atccatccgg tgaagaacgc tgcataccng caaactatgc gaagcaatat gtccggcaca agcnataacg attgaggctg aagcaaggcc cgatggaagt cggcgaacta ctcgttatga tattgacatg acaaaatgta tntattgtgg tttatgtcag gaggcttgtc cngttgatgc cattgttgag ggtccgaatt ttgaatactc nacggagact catgananct gctatataac anagaaaaac ttttgttgaa tggtgatcga tgggagccag aatnnnctgc naacttacaa gctgaatatc tttatcgtta agtgagtaag ataggannnn gctgcnannn nnncccna

Expected Protein Sequence

MAHHHHHHMR SWFCLAYHSG NILSKLPFPA NCSQIRTTYK YVAESAKSDG TLLGDFRKAM HYMFMTELIR GMGIIVGHYF MEPATINYPF EKGPLSSRFR GEHALRRYPS GEERCIACKL CEAICPAQAI TIEAEARPDG SRRTTRYDID MTKCIYCGLC QEACPVDAIV EGPNFEYSTE THELLYNKEK LLLNGDRWEP ELAANLQAEY LYR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtt catggttttg cttggcatat cactctggga acattctcag taaacttcca tttccagcaa actgttcgca aattcgcaca acatacaagt atgttgctga atcagcgaaa tccgacggaa cactgctggg tgatttccgc aaagcaatgc attatatgtt catgacagag cttataagag gtatgggtat aattgttggt cattacttta tggagcctgc aacaataaat tatccattcg agaaaggtcc tttgagctcg cgttttcgtg gtgagcatgc acttcggagg tatccatccg gtgaagaacg ctgcatagcc tgcaaactat gcgaagcaat atgtccggca caagcaataa cgattgaggc tgaagcaagg cccgatggaa gtcggcgaac tactcgttat gatattgaca tgacaaaatg tatttattgt ggtttatgtc aggaggcttg tccagttgat gccattgttg agggtccgaa ttttgaatac tcaacggaga ctcatgarga gctgctatat aacaaagaaa aacttttgtt gaatggtgat cgatgggagc cagaattagc tgcaaactta caagctgaat atctttatcg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat