BrcaA.18826.a

BspH VirB type IV secretion effector protein

CENTER ID: BrcaA.18826.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.18826.a.A1.GE39603 full length 1 479
BrcaA.18826.a.B1.GE38133 full length 1 479
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|483179.4.peg.1890
RefSeq: YP_001593662.1
UniProt: A9M893

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASKTTLNAK NLEALGAKRL AELLIEISTG NANHKRLLRM ELVGNSSGAE LAREVRKRLG SIGRSKSWID WQKAKSVRAD LEAQRKSIAE KIAPTDPNEA FELIWQFLSL ADPIFERSCD GSGALIESFH AACEDAGRIA AMAKVPIDRL VEKIFSVLQD NGYGQYDALI EAMAPALGEA GLRKLQRAFE AWAKEPQPKV ASKDKEVIAW SSDGPVYRDQ LYASGKSITV KVVLQQVADA LGDVDLYIEQ QSKESQTFAT VTTDIARRLL AAGRAEDALT ALEKTKFDSC REIPFEWQIL RAEVLESLGR AEEAQQFRWK SFEQNLNDEL LREYVKRLPD FDDVEAEEKA FAFVRAVADA NHALYFFLQY PSLSEASKLV VSRASEMDGD HYELMSDAAE KLSAKFPLAA TILLRAMIDF TLNNARSSRY KHTARHLLEC GALSRSIEDY HSFAPHEAYI ADLRKMHGRK HGFWPLVDR
NT Sequence
atggcatcaa agactacctt gaatgcgaag aaccttgaag cacttggcgc taagcgcctt gctgagctat tgattgagat cagcaccggg aatgcgaacc ataagcgctt gcttcgtatg gaacttgtcg gaaattcgag tggtgccgag ttggcaaggg aagttcgtaa gcgtttgggc agtatcggac gttcaaaaag ctggattgac tggcagaagg caaagtccgt tagggctgat ctggaagccc agcgcaaatc tattgccgag aaaattgcgc ccactgatcc gaatgaggcg tttgaactga tctggcagtt tctatcgctc gcagacccga tttttgagcg ttcatgcgat ggaagtggcg cgttgatcga aagcttccat gccgcgtgcg aagatgctgg tcgcattgct gcaatggcaa aagtccccat tgaccgcttg gtcgagaaaa tcttctcggt cttgcaggac aatggatatg gtcaatatga tgcgctgatt gaggcaatgg ctccggctct cggtgaggcc ggcttgcgaa agcttcaacg agccttcgaa gcatgggcta aagagcccca gccaaaagtc gcaagcaaag acaaagaggt tattgcttgg agtagcgatg gccctgttta ccgcgatcag ctttatgcca gcggtaagtc gatcacggtc aaagttgtgc ttcagcaggt cgccgatgct cttggcgatg ttgatctcta tattgaacag caatctaaag aatcacagac gttcgctaca gtcaccacag atatcgctcg gaggctttta gccgctggtc gcgcggaaga cgcattaacc gctctggaga aaacaaaatt cgatagctgt cgggaaatcc cttttgaatg gcaaattttg cgtgctgagg tgctggaatc tctcggacgt gcagaagagg cgcaacagtt tcggtggaag agtttcgagc agaatctcaa tgatgagttg ctacgggaat atgtgaagcg acttcccgat tttgatgacg ttgaagctga agaaaaggca tttgcatttg tccgagccgt cgccgatgca aaccatgcac tctatttctt ccttcaatat ccatcactta gtgaagcgtc taagctggtg gtcagtcgag caagtgagat ggatggcgat cattatgagc tgatgtctga tgccgcagag aagctatcgg ccaaattccc tttggccgcg actattctgt tacgcgcaat gatagatttc acgctcaata acgctcgctc aagtcgttac aagcacacag cacggcattt gcttgagtgt ggtgctctgt cccgtagcat cgaagattat cattccttcg cgccgcatga ggcttatatt gctgatctcc gaaagatgca cgggaggaaa catggcttct ggccgcttgt agatcggtga
Details for BrcaA.18826.a.A1.GE39603
HARVESTED ON: 10/30/2015
SEQUENCED ON: 10/23/2015
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASKTTLNA KNLEALGAKR LAELLIEIST GNANHKRLLR MELVGNSSGA ELAREVRKRL GSIGRSKSWI DWQKAKSVRA DLEAQRKSIA EKIAPTDPNE AFELIWQFLS LADPIFERSC DGSGALIESF HAACEDAGRI AAMAKVPIDR LVEKIFSVLQ DNGYGQYDAL IEAMAPALGE AGLRKLQRAF EAWAKEPQPK VASKDKEVIA WSSDGPVYRD QLYASGKSIT VKVVLQQVAD ALGDVDLYIE QQSKESQTFA TVTTDIARRL LAAGRAEDAL TALEKTKFDS CREIPFEWQI LRAEVLESLG RAEEAQQFRW KSFEQNLNDE LLREYVKRLP DFDDVEAEEK AFAFVRAVAD ANHALYFFLQ YPSLSEASKL VVSRASEMDG DHYELMSDAA EKLSAKFPLA ATILLRAMID FTLNNARSSR YKHTARHLLE CGALSRSIED YHSFAPHEAY IADLRKMHGR KHGFWPLV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcat caaagactac cttgaatgcg aagaaccttg aagcacttgg cgctaagcgc cttgctgagc tattgattga gatcagcacc gggaatgcga accataagcg cttgcttcgt atggaacttg tcggaaattc gagtggtgcc gagttggcaa gggaagttcg taagcgtttg ggcagtatcg gacgttcaaa aagctggatt gactggcaga aggcaaagtc cgttagggct gatctggaag cccagcgcaa atctattgcc gagaaaattg cgcccactga tccgaatgag gcgtttgaac tgatctggca gtttctatcg ctcgcagacc cgatttttga gcgttcatgc gatggaagtg gcgcgttgat cgaaagcttc catgccgcgt gcgaagatgc tggtcgcatt gctgcaatgg caaaagtccc cattgaccgc ttggtcgaga aaatcttctc ggtcttgcag gacaatggat atggtcaata tgatgcgctg attgaggcaa tggctccggc tctcggtgag gccggcttgc gaaagcttca acgagccttc gaagcatggg ctaaagagcc ccagccaaaa gtcgcaagca aagacaaaga ggttattgct tggagtagcg atggccctgt ttaccgcgat cagctttatg ccagcggtaa gtcgatcacg gtcaaagttg tgcttcagca ggtcgccgat gctcttggcg atgttgatct ctatattgaa cagcaatcta aagaatcaca gacgttcgct acagtcacca cagatatcgc tcggaggctt ttagccgctg gtcgcgcgga agacgcatta accgctctgg agaaaacaaa attcgatagc tgtcgggaaa tcccttttga atggcaaatt ttgcgtgctg aggtgctgga atctctcgga cgtgcagaag aggcgcaaca gtttcggtgg aagagtttcg agcagaatct caatgatgag ttgctacggg aatatgtgaa gcgacttccc gattttgatg acgttgaagc tgaagaaaag gcatttgcat ttgtccgagc cgtcgccgat gcaaaccatg cactctattt cttccttcaa tatccatcac ttagtgaagc gtctaagctg gtggtcagtc gagcaagtga gatggatggc gatcattatg agctgatgtc tgatgccgca gagaagctat cggccaaatt ccctttggcc gcgactattc tgttacgcgc aatgatagat ttcacgctca ataacgctcg ctcaagtcgt tacaagcaca cagcacggca tttgcttgag tgtggtgctc tgtcccgtag catcgaagat tatcattcct tcgcgccgca tgaggcttat attgctgatc tccgaaagat gcacgggagg aaacatggct tctggccgct tgtagat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASKTTLNA KNLEALGAKR LAELLIEIST GNANHKRLLR MELVGNSSGA ELAREVRKRL GSIGRSKSWI DWQKAKSVRA DLEAQRKSIA EKIAPTDPNE AFELIWQFLS LADPIFERSC DGSGALIESF HAACEDAGRI AAMAKVPIDR LVEKIFSVLQ DNGYGQYDAL IEAMAPALGE AGLRKLQRAF EAWAKEPQPK VASKDKEVIA WSSDGPVYRD QLYASGKSIT VKVVLQQVAD ALGDVDLYIE QQSKESQTFA TVTTDIARRL LAAGRAEDAL TALEKTKFDS CREIPFEWQI LRAEVLESLG RAEEAQQFRW KSFEQNLNDE LLREYVKRLP DFDDVEAEEK AFAFVRAVAD ANHALYFFLQ YPSLSEASKL VVSRASEMDG DHYELMSDAA EKLSAKFPLA ATILLRAMID FTLNNARSSR YKHTARHLLE CGALSRSIED YHSFAPHEAY IADLRKMHGR KHGFWPLVDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatcaaag actaccttga atgcgaagaa ccttgaagca cttggcgcta agcgccttgc tgagctattg attgagatca gcaccgggaa tgcgaaccat aagcgcttgc ttcgtatgga acttgtcgga aattcgagtg gtgccgagtt ggcaagggaa gttcgtaagc gtttgggcag tatcggacgt tcaaaaagct ggattgactg gcagaaggca aagtccgtta gggctgatct ggaagcccag cgcaaatcta ttgccgagaa aattgcgccc actgatccga atgaggcgtt tgaactgatc tggcagtttc tatcgctcgc agacccgatt tttgagcgtt catgcgatgg aagtggcgcg ttgatcgaaa gcttccatgc cgcgtgcgaa gatgctggtc gcattgctgc aatggcaaaa gtccccattg accgcttggt cgagaaaatc ttctcggtct tgcaggacaa tggatatggt caatatgatg cgctgattga ggcaatggct ccggctctcg gtgaggccgg cttgcgaaag cttcaacgag ccttcgaagc atgggctaaa gagccccagc caaaagtcgc aagcaaagac aaagaggtta ttgcttggag tagcgatggc cctgtttacc gcgatcagct ttatgccagc ggtaagtcga tcacggtcaa agttgtgctt cagcaggtcg ccgatgctct tggcgatgtt gatctctata ttgaacagca atctaaagaa tcacagacgt tcgctacagt caccacagat atcgctcgga ggcttttagc cgctggtcgc gcggaagacg cattaaccgc tctggagaaa acaaaattcg atagctgtcg ggaaatccct tttgaatggc aaattttgcg tgctgaggtg ctggaatctc tcggacgtgc agaagaggcg caacagtttc ggtggaagag tttcgagcag aatctcaatg atgagttgct acgggaatat gtgaagcgac ttcccgattt tgatgacgtt gaagctgaag aaaaggcatt tgcatttgtc cgagccgtcg ccgatgcaaa ccatgcactc tatttcttcc ttcaatatcc atcacttagt gaagcgtcta agctggtggt cagtcgagca agtgagatgg atggcgatca ttatgagctg atgtctgatg ccgcagagaa gctatcggcc aaattccctt tggccgcgac tattctgtta cgcgcaatga tagatttcac gctcaataac gctcgctcaa gtcgttacaa gcacacagca cggcatttgc ttgagtgtgg tgctctgtcc cgtagcatcg aagattatca ttccttcgcg ccgcatgagg cttatattgc tgatctccga aagatgcacg ggaggaaaca tggcttctgg ccgcttgtag atcggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BrcaA.18826.a.B1.GE38133
HARVESTED ON: 6/11/2014
SEQUENCED ON: 6/12/2014
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 70
Validated AA Sequence
MLSXAAMXKV PIDRLVEKIF SVLQDNGYGQ YDALIEAMAP ALGEAGLRKL QRXFEAWAKE PQPKVASKDK EVIAWSSDGP VYRDQLYASG KSITVKVVLQ QVADALGDVD LYIEQQSKES QTFATVTTDI ARRLLAAGRA EDALTALEKT KFDSCREIPF EWQILRAEVL ESLGRAEEAQ QFRWKSFEQN LNDELLREYV KRLPDFDDVE AEEKAFAFVR AVADANHALY FFLQYPSLSE ASKLVVSRAS EMDGDHYEPM SDAAEKLSAK FPLAATILLR AMIDFTLNNA RSSRYKHTAR HLLECGALSR SIEDYHSFAP HEAYIADLRK MHGRKHGFWP LVDR
Validated NT Sequence
gcttnnttcg ggctttgtta gcagccggat cctatcttac tcacttaccg atctacaagc ggccagaagc catgtttcct cccgtgcatc tttcggagat cagcaatata agcctcatgc ggcgcgaagg aatgataatc ttcgatgcta cgggacagag caccacactc aagcaaatgc cgtgctgtgt gcttgtaacg acttgagcga gcgttattga gcgtgaaatc tatcattgcg cgtaacagaa tagtcgcggc caaagggaat ttggccgata gcttctctgc ggcatcagac atcggctcat aatgatcgcc gtccatctca cttgcacgac tgaccaccag cttagacgct tcactaagtg atggatattg aaggaagaaa tagagtgcat ggtttgcatc ggcgacggct cggacaaatg caaatgcctt ttcttcagct tcaacgtcat caaaatcggg aagtcgcttc acatattccc gtagcaactc atcattgaga ttctgctcga aactcttcca ccgaaactgt tgcgcctctt ctgcacgtcc gagagattcc agcacctcag cacgcaaaat ttgccattca aaagggattt cccgacagct atcgaatttt gttttctcca gagcggttaa tgcgtcttcc gcgcgaccag cggctaaaag cctccgagcg atatctgtgg tgactgtagc gaacgtctgt gattctttag attgctgttc aatatagaga tcaacatcgc caagagcatc ggcgacctgc tgaagcacaa ctttgaccgt gatcgactta ccgctggcat aaagctgatc gcggtaaaca gggccatcgc tactccaagc aataacctct ttgtctttgc ttgcgacttt tggctggggc tctttagccc atgcttcgaa ggntcgttga agctttcgca agccggcctc accgagagcc ggagccattg cctcaatcag cgcatcatat tgaccatatc cattgtcctg caagaccgag aagattttct cgaccaagcg gtcaatgggg acttttgnca ttgcagcang cgacagcatc ttcgcacgnn natnnncttt cgatcangcn cncttncatc gcatgnngct caaaancggg ngcnannnnn gaannnnnna tcannnaacn nntnnnncgn tcnnngnntt tcnnnnnnag nnnnnnncct gngnntttnc ngaaatcnna nc
Expected Protein Sequence
MAHHHHHHMA SKTTLNAKNL EALGAKRLAE LLIEISTGNA NHKRLLRMEL VGNSSGAELA REVRKRLGSI GRSKSWIDWQ KAKSVRADLE AQRKSIAEKI APTDPNEAFE LIWQFLSLAD PIFERSCDGS GALIESFHAA CEDAGRIAAM AKVPIDRLVE KIFSVLQDNG YGQYDALIEA MAPALGEAGL RKLQRAFEAW AKEPQPKVAS KDKEVIAWSS DGPVYRDQLY ASGKSITVKV VLQQVADALG DVDLYIEQQS KESQTFATVT TDIARRLLAA GRAEDALTAL EKTKFDSCRE IPFEWQILRA EVLESLGRAE EAQQFRWKSF EQNLNDELLR EYVKRLPDFD DVEAEEKAFA FVRAVADANH ALYFFLQYPS LSEASKLVVS RASEMDGDHY ELMSDAAEKL SAKFPLAATI LLRAMIDFTL NNARSSRYKH TARHLLECGA LSRSIEDYHS FAPHEAYIAD LRKMHGRKHG FWPLVDR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcat caaagactac cttgaatgcg aagaaccttg aagcacttgg cgctaagcgc cttgctgagc tattgattga gatcagcacc gggaatgcga accataagcg cttgcttcgt atggaacttg tcggaaattc gagtggtgcc gagttggcaa gggaagttcg taagcgtttg ggcagtatcg gacgttcaaa aagctggatt gactggcaga aggcaaagtc cgttagggct gatctggaag cccagcgcaa atctattgcc gagaaaattg cgcccactga tccgaatgag gcgtttgaac tgatctggca gtttctatcg ctcgcagacc cgatttttga gcgttcatgc gatggaagtg gcgcgttgat cgaaagcttc catgccgcgt gcgaagatgc tggtcgcatt gctgcaatgg caaaagtccc cattgaccgc ttggtcgaga aaatcttctc ggtcttgcag gacaatggat atggtcaata tgatgcgctg attgaggcaa tggctccggc tctcggtgag gccggcttgc gaaagcttca acgagccttc gaagcatggg ctaaagagcc ccagccaaaa gtcgcaagca aagacaaaga ggttattgct tggagtagcg atggccctgt ttaccgcgat cagctttatg ccagcggtaa gtcgatcacg gtcaaagttg tgcttcagca ggtcgccgat gctcttggcg atgttgatct ctatattgaa cagcaatcta aagaatcaca gacgttcgct acagtcacca cagatatcgc tcggaggctt ttagccgctg gtcgcgcgga agacgcatta accgctctgg agaaaacaaa attcgatagc tgtcgggaaa tcccttttga atggcaaatt ttgcgtgctg aggtgctgga atctctcgga cgtgcagaag aggcgcaaca gtttcggtgg aagagtttcg agcagaatct caatgatgag ttgctacggg aatatgtgaa gcgacttccc gattttgatg acgttgaagc tgaagaaaag gcatttgcat ttgtccgagc cgtcgccgat gcaaaccatg cactctattt cttccttcaa tatccatcac ttagtgaagc gtctaagctg gtggtcagtc gagcaagtga gatggatggc gatcattatg agctgatgtc tgatgccgca gagaagctat cggccaaatt ccctttggcc gcgactattc tgttacgcgc aatgatagat ttcacgctca ataacgctcg ctcaagtcgt tacaagcaca cagcacggca tttgcttgag tgtggtgctc tgtcccgtag catcgaagat tatcattcct tcgcgccgca tgaggcttat attgctgatc tccgaaagat gcacgggagg aaacatggct tctggccgct tgtagatcgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat