BrcaA.18158.b

Transcriptional regulator, GntR family

CENTER ID: BrcaA.18158.b
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.18158.b.B1.GE38313 Full length( BrcaA.18158.b ) 1 257
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.18158.b.B1.PW37595 Full length( BrcaA.18158.b ) 1 257

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_3027
BV-BRC: fig|483179.4.peg.3027
RefSeq: YP_001594758.1
UniProt: A9MCA0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRGRSAQPAL EKQKNVADTE SGAATGVKAR TLPSRVAGVQ RVTTAKLIYH ELQQQIIRME LLPGTPLNEK ALTEKYGVSR TPVREALIRL AEDRLVDVFP QSGTFVARIP VDAIPEAVVI RQALEGETAE RAAANSTTAA IEKLDELIHL QTFYARKDKP GPFHETDDAF HETIAEIAGY PGIWQHLKPV KMQIDRARRM TMPILRRMEQ VLREHHAIRD AISARDVHAA REAMKHHLSA VLPDIDELRK SRPDYFA
NT Sequence
ATGAGAGGAC GCAGTGCGCA ACCGGCGCTT GAAAAACAGA AAAATGTGGC GGACACCGAG AGCGGTGCGG CCACAGGCGT AAAGGCGCGC ACCTTGCCTT CGCGGGTGGC CGGGGTACAG CGCGTCACGA CAGCCAAGCT CATCTATCAT GAACTGCAAC AGCAAATTAT CCGGATGGAG CTTCTTCCCG GCACACCGCT CAATGAAAAG GCGTTGACCG AAAAATATGG CGTCAGCCGC ACGCCGGTAC GGGAAGCATT GATCCGCCTT GCCGAAGATC GTCTGGTCGA TGTTTTCCCG CAATCGGGTA CATTCGTGGC GCGCATTCCG GTTGATGCTA TCCCGGAAGC TGTTGTCATA CGACAGGCGC TGGAGGGAGA AACGGCAGAA AGGGCTGCCG CCAATTCAAC CACAGCCGCT ATCGAGAAGC TGGACGAGTT GATCCACTTG CAGACTTTTT ATGCCCGCAA GGACAAACCC GGTCCATTTC ATGAAACCGA TGACGCTTTC CACGAAACAA TCGCGGAAAT TGCTGGCTAT CCCGGCATTT GGCAGCATCT CAAGCCGGTG AAAATGCAGA TTGACCGCGC CCGCCGCATG ACCATGCCGA TTCTCAGGCG AATGGAGCAG GTGTTGCGTG AACATCATGC CATTCGCGAT GCCATCTCGG CCCGTGATGT CCATGCCGCG CGCGAAGCCA TGAAACATCA TTTAAGTGCT GTTTTGCCGG ATATTGACGA GTTGCGCAAA AGCCGTCCGG ACTATTTTGC T
Details for BrcaA.18158.b.B1.GE38313
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTTAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILRRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Validated NT Sequence
annccntcna nannttttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gagaggacgc agtgcgcaac cggcgcttga aaaacagaaa aatgtggcgg acaccgagag cggtgcggcc acaggcgtaa aggcgcgcac cttgccttcg cgggtggccg gggtacagcg cgtcacgaca gccaagctca tctatcatga actgcaacag caaattatcc ggatggagct tcttcccggc acaccgctca atgaaaaggc gttgaccgaa aaatatggcg tcagccgcac gccggtacgg gaagcattga tccgccttgc cgaagatcgt ctggtcgatg ttttcccgca atcgggtaca ttcgtggcgc gcattccggt tgatgctatc ccggaagctg ttgtcatacg acaggcgctg gagggagaaa cggcagaaag ggctgccgcc aattcaacca cagccgctat cgagaagctg gacgagttga tccacttgca gactttttat gcccgcaagg acaaacccgg tccatttcat gaaaccgatg acgctttcca cgaaacaatc gcggaaattg ctggctatcc cggcatttgg cagcatctca agccggtgaa aatgcagatt gaccgcgccc gccgcatgac catgccgatt ctcaggcgaa tggagcaggt gttgcgtgaa catcatgcca ttcgcgatgc catctcggcc cgtgatgtcc atgccgcgcg cgaagccatg aaacatcatt taagtgctgt tttgccggat attgacgagt tgcgcaaaag ccgtccggac tattttgctt aagtgagtaa gataggatcc nnnnnnnccc ncccnnnnnn nnnnnnnnnn nnaanaanaa aagnannnna nnngtctaaa naaagaagtt nttnttgggt agatccagag attatctggt tcacgnntac tctactgang gagatctcgt anncnctcnn tattctagtg aatgaaaaaa naaggctgcg nctnnnanag antgcaagna ggcggtcttt tcttaatctg ttggnagcaa tccttttgga tcccttgccc cttganttgg ctaattggtn gagnntcccn anagtannnn ctaanncctc caacgtcnan ggngnaaatt tcaacngnnt gnactnntnn tannaanngg ngnttnaaac acacctcttn naatgacccn nnctacaaan nnnngngngg agnnnggtan cnnnnttctc
Expected Protein Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTTAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILRRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagag gacgcagtgc gcaaccggcg cttgaaaaac agaaaaatgt ggcggacacc gagagcggtg cggccacagg cgtaaaggcg cgcaccttgc cttcgcgggt ggccggggta cagcgcgtca cgacagccaa gctcatctat catgaactgc aacagcaaat tatccggatg gagcttcttc ccggcacacc gctcaatgaa aaggcgttga ccgaaaaata tggcgtcagc cgcacgccgg tacgggaagc attgatccgc cttgccgaag atcgtctggt cgatgttttc ccgcaatcgg gtacattcgt ggcgcgcatt ccggttgatg ctatcccgga agctgttgtc atacgacagg cgctggaggg agaaacggca gaaagggctg ccgccaattc aaccacagcc gctatcgaga agctggacga gttgatccac ttgcagactt tttatgcccg caaggacaaa cccggtccat ttcatgaaac cgatgacgct ttccacgaaa caatcgcgga aattgctggc tatcccggca tttggcagca tctcaagccg gtgaaaatgc agattgaccg cgcccgccgc atgaccatgc cgattctcag gcgaatggag caggtgttgc gtgaacatca tgccattcgc gatgccatct cggcccgtga tgtccatgcc gcgcgcgaag ccatgaaaca tcatttaagt gctgttttgc cggatattga cgagttgcgc aaaagccgtc cggactattt tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BrcaA.18158.b.B1.PW37595
PURIFICATION DATe: 11/11/2014
CONCENTRATION: 4mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTTAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILRRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Validated NT Sequence
annccntcna nannttttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat gagaggacgc agtgcgcaac cggcgcttga aaaacagaaa aatgtggcgg acaccgagag cggtgcggcc acaggcgtaa aggcgcgcac cttgccttcg cgggtggccg gggtacagcg cgtcacgaca gccaagctca tctatcatga actgcaacag caaattatcc ggatggagct tcttcccggc acaccgctca atgaaaaggc gttgaccgaa aaatatggcg tcagccgcac gccggtacgg gaagcattga tccgccttgc cgaagatcgt ctggtcgatg ttttcccgca atcgggtaca ttcgtggcgc gcattccggt tgatgctatc ccggaagctg ttgtcatacg acaggcgctg gagggagaaa cggcagaaag ggctgccgcc aattcaacca cagccgctat cgagaagctg gacgagttga tccacttgca gactttttat gcccgcaagg acaaacccgg tccatttcat gaaaccgatg acgctttcca cgaaacaatc gcggaaattg ctggctatcc cggcatttgg cagcatctca agccggtgaa aatgcagatt gaccgcgccc gccgcatgac catgccgatt ctcaggcgaa tggagcaggt gttgcgtgaa catcatgcca ttcgcgatgc catctcggcc cgtgatgtcc atgccgcgcg cgaagccatg aaacatcatt taagtgctgt tttgccggat attgacgagt tgcgcaaaag ccgtccggac tattttgctt aagtgagtaa gataggatcc nnnnnnnccc ncccnnnnnn nnnnnnnnnn nnaanaanaa aagnannnna nnngtctaaa naaagaagtt nttnttgggt agatccagag attatctggt tcacgnntac tctactgang gagatctcgt anncnctcnn tattctagtg aatgaaaaaa naaggctgcg nctnnnanag antgcaagna ggcggtcttt tcttaatctg ttggnagcaa tccttttgga tcccttgccc cttganttgg ctaattggtn gagnntcccn anagtannnn ctaanncctc caacgtcnan ggngnaaatt tcaacngnnt gnactnntnn tannaanngg ngnttnaaac acacctcttn naatgacccn nnctacaaan nnnngngngg agnnnggtan cnnnnttctc
Expressed Protein Sequence
MAHHHHHHMR GRSAQPALEK QKNVADTESG AATGVKARTL PSRVAGVQRV TTAKLIYHEL QQQIIRMELL PGTPLNEKAL TEKYGVSRTP VREALIRLAE DRLVDVFPQS GTFVARIPVD AIPEAVVIRQ ALEGETAERA AANSTTAAIE KLDELIHLQT FYARKDKPGP FHETDDAFHE TIAEIAGYPG IWQHLKPVKM QIDRARRMTM PILRRMEQVL REHHAIRDAI SARDVHAARE AMKHHLSAVL PDIDELRKSR PDYFA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAG GACGCAGTGC GCAACCGGCG CTTGAAAAAC AGAAAAATGT GGCGGACACC GAGAGCGGTG CGGCCACAGG CGTAAAGGCG CGCACCTTGC CTTCGCGGGT GGCCGGGGTA CAGCGCGTCA CGACAGCCAA GCTCATCTAT CATGAACTGC AACAGCAAAT TATCCGGATG GAGCTTCTTC CCGGCACACC GCTCAATGAA AAGGCGTTGA CCGAAAAATA TGGCGTCAGC CGCACGCCGG TACGGGAAGC ATTGATCCGC CTTGCCGAAG ATCGTCTGGT CGATGTTTTC CCGCAATCGG GTACATTCGT GGCGCGCATT CCGGTTGATG CTATCCCGGA AGCTGTTGTC ATACGACAGG CGCTGGAGGG AGAAACGGCA GAAAGGGCTG CCGCCAATTC AACCACAGCC GCTATCGAGA AGCTGGACGA GTTGATCCAC TTGCAGACTT TTTATGCCCG CAAGGACAAA CCCGGTCCAT TTCATGAAAC CGATGACGCT TTCCACGAAA CAATCGCGGA AATTGCTGGC TATCCCGGCA TTTGGCAGCA TCTCAAGCCG GTGAAAATGC AGATTGACCG CGCCCGCCGC ATGACCATGC CGATTCTCAG GCGAATGGAG CAGGTGTTGC GTGAACATCA TGCCATTCGC GATGCCATCT CGGCCCGTGA TGTCCATGCC GCGCGCGAAG CCATGAAACA TCATTTAAGT GCTGTTTTGC CGGATATTGA CGAGTTGCGC AAAAGCCGTC CGGACTATTT TGCTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT