BrcaA.18134.a

Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3)

CENTER ID: BrcaA.18134.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.18134.a.B1.GE38311 Full length( BrcaA.18134.a ) 1 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_0209
BV-BRC: fig|483179.4.peg.209
RefSeq: YP_001592072.1
UniProt: A9M7E2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVQEKQFDIF IIGGGINGCG IARDAVGRGF TVGLAEMNDL ASGTSSRATK LIHGGLRYLE HYEFRLVCEA LMEREVLWAN APHIIHPMRF VLPYHKGGLR PAWLLRLGLF LYDHLGGRKK LPATRTLNMR TDKAGEPLKP LFTKAFEYSD CWVDDARFVA LTARDAADRG AKIATRTSVV AASRDGQGWT ITLEDTGTGR RENVHARLLV KAAGPWADKV LQGVEGDRQL HNIRLVQGSH IVVRRKFSDP RAYFFQNNDG RIIFAIPYED DFTLIGTTDQ DYKGDPAKVA ITDSETDYLC QAASEYFREP VRREDIVWTY SGVRPLYDDG ASKAQEATRD YVLKEDAPDG LAPLINVFGG KLTTARKLAE HMLQKIEHRL GRKGAPWTHV APLPGGDFED VAFETELKKL EAAYPFLDAR HARRLFRLYG TRAYKLLGQA SSLGDLGRHF GSDLYEAEVR YLVENEWARS AEDILWRRTK LGLRLTAAEV AAVQGFVEPA IAA
NT Sequence
ATGGTGCAGG AAAAACAATT CGATATTTTT ATTATAGGTG GCGGCATCAA TGGCTGCGGC ATTGCCCGTG ATGCGGTGGG GCGGGGCTTC ACCGTTGGGC TGGCCGAGAT GAACGATCTG GCAAGCGGCA CCTCTTCGCG CGCAACCAAG CTCATTCACG GTGGTTTGCG CTATCTGGAA CATTATGAAT TCCGGCTGGT GTGCGAGGCG CTGATGGAGC GCGAGGTGCT GTGGGCCAAT GCGCCGCATA TCATCCATCC GATGCGTTTC GTGCTGCCTT ATCATAAGGG CGGCCTACGT CCCGCCTGGC TGTTGCGGCT TGGCCTGTTT CTTTACGACC ATCTGGGCGG CCGCAAGAAG CTTCCCGCCA CGCGCACGCT CAACATGCGC ACCGACAAGG CCGGCGAACC GTTGAAACCG CTTTTCACCA AGGCTTTTGA ATATTCCGAT TGCTGGGTGG ACGATGCGCG CTTCGTGGCG CTCACCGCCC GCGATGCGGC TGATCGCGGC GCGAAGATCG CGACCCGAAC GAGCGTCGTT GCCGCAAGCC GCGACGGGCA GGGCTGGACC ATCACACTGG AAGATACCGG CACCGGCAGG CGTGAGAACG TACACGCCCG CCTTCTGGTC AAAGCCGCCG GGCCGTGGGC CGACAAGGTT CTGCAAGGTG TGGAAGGCGA CCGGCAATTG CACAATATCC GTCTGGTGCA GGGCAGCCAT ATCGTCGTGC GCAGGAAGTT TTCCGACCCG CGCGCCTATT TCTTCCAGAA CAACGACGGG CGCATCATCT TCGCCATTCC TTATGAAGAT GATTTCACCC TGATTGGCAC CACGGATCAG GATTATAAGG GCGACCCGGC CAAGGTGGCG ATCACCGACA GCGAGACCGA TTATCTCTGC CAGGCGGCAA GCGAATATTT CCGCGAGCCG GTGCGCCGGG AGGATATCGT CTGGACCTAT TCCGGCGTGC GCCCGCTCTA TGATGACGGG GCCAGCAAGG CGCAGGAGGC AACCCGCGAC TATGTGCTGA AGGAAGATGC GCCAGATGGT CTGGCGCCGC TCATCAATGT TTTCGGCGGC AAGCTCACCA CAGCACGCAA GCTTGCCGAA CATATGCTGC AGAAAATCGA GCACCGGCTT GGCCGCAAGG GCGCCCCATG GACCCATGTA GCACCATTGC CAGGCGGGGA TTTCGAGGAT GTGGCTTTTG AAACGGAACT GAAGAAGCTT GAGGCAGCCT ATCCTTTCCT TGATGCGCGC CATGCGCGCC GCCTGTTCCG GCTCTATGGC ACCCGGGCAT ATAAGCTTTT GGGGCAGGCT TCTTCGCTGG GCGATCTTGG CCGTCATTTT GGCAGCGATC TCTATGAGGC GGAAGTGCGC TATCTTGTCG AAAATGAATG GGCGCGAAGC GCGGAAGATA TTTTGTGGCG GCGCACCAAG CTCGGCCTGC GCCTGACGGC GGCGGAAGTC GCGGCCGTGC AGGGCTTTGT GGAGCCTGCA ATCGCCGCC
Details for BrcaA.18134.a.B1.GE38311
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 57kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMV QEKQFDIFII GGGINGCGIA RDAVGRGFTV GLAEMNDLAS GTSSRATKLI HGGLRYLEHY EFRLVCEALM EREVLWANAP HIIHPMRFVL PYHKGGLRPA WLLRLGLFLY DHLGGRKKLP ATRTLNMRTD KAGEPLKPLF TKAFEYSDCW VDDARFVALT ARDAADRGAK IATRTSVVAA SRDGQGWTIT LEDTGTGRRE NVHARLLVKA AGPWADKVLQ GVEGDRQLHN IRLVQGSHIV VRRKFSDPRA YFFQNNDXRI IFAIPYEDDF TLIGTTDHDY KATRPRWRSP TARPIISARR QANISASRCA XXYRLDLXXC ARSMMTGXXA EHPRLXAXXM XXXXXPLIXX XXXXSPXASX XXXXXXXXXX XXS
Validated NT Sequence
tcnntnnata ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggtg caggaaaaac aattcgatat ttttattata ggtggcggca tcaatggctg cggcattgcc cgtgatgcgg tggggcgggg cttcaccgtt gggctggccg agatgaacga tctggcaagc ggcacctctt cgcgcgcaac caagctcatt cacggtggtt tgcgctatct ggaacattat gaattccggc tggtgtgcga ggcgctgatg gagcgcgagg tgctgtgggc caatgcgccg catatcatcc atccgatgcg tttcgtgctg ccttatcata agggcggcct acgtcccgcc tggctgttgc ggcttggcct gtttctttac gaccatctgg gcggccgcaa gaagcttccc gccacgcgca cgctcaacat gcgcaccgac aaggccggcg aaccgttgaa accgcttttc accaaggctt ttgaatattc cgattgctgg gtggacgatg cgcgcttcgt ggcgctcacc gcccgcgatg cggctgatcg cggcgcgaag atcgcgaccc gaacgagcgt cgttgccgca agccgcgacg ggcagggctg gaccatcaca ctggaagata ccggcaccgg caggcgtgag aacgtacacg cccgccttct ggtcaaagcc gccgggccgt gggccgacaa ggttctgcaa ggtgtggaag gcgaccggca attgcacaat atccgtctgg tgcagggcag ccatatcgtc gtgcgcagga agttttccga cccgcgcgcc tatttcttcc agaacaacga cnggcgcatc atcttcgcca ttccttatga agatgatttc accctgattg gcaccacgga tcacgattat aaggcgaccc ggccaaggtg gcgatcaccg acagcgagac cgattatctc tgccaggcgg caagcgaata tttccgcgag ccggtgcgcn nnnngatatc gtctggacct annnncgtgc gcccgctcta tgatgacggg cngcagngca gagcacccgc gactatntgc tgannanatg cncngatngn cngnnccgct catcangntt ncgnngnnag nctcaccnna ngcnagcnnn cnannnnnct gnnnaaannn nnnnnnnncn nngncctcnt gancccnnnn nnnncatntc nangnc
Expected Protein Sequence
MAHHHHHHMV QEKQFDIFII GGGINGCGIA RDAVGRGFTV GLAEMNDLAS GTSSRATKLI HGGLRYLEHY EFRLVCEALM EREVLWANAP HIIHPMRFVL PYHKGGLRPA WLLRLGLFLY DHLGGRKKLP ATRTLNMRTD KAGEPLKPLF TKAFEYSDCW VDDARFVALT ARDAADRGAK IATRTSVVAA SRDGQGWTIT LEDTGTGRRE NVHARLLVKA AGPWADKVLQ GVEGDRQLHN IRLVQGSHIV VRRKFSDPRA YFFQNNDGRI IFAIPYEDDF TLIGTTDQDY KGDPAKVAIT DSETDYLCQA ASEYFREPVR REDIVWTYSG VRPLYDDGAS KAQEATRDYV LKEDAPDGLA PLINVFGGKL TTARKLAEHM LQKIEHRLGR KGAPWTHVAP LPGGDFEDVA FETELKKLEA AYPFLDARHA RRLFRLYGTR AYKLLGQASS LGDLGRHFGS DLYEAEVRYL VENEWARSAE DILWRRTKLG LRLTAAEVAA VQGFVEPAIA A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc aggaaaaaca attcgatatt tttattatag gtggcggcat caatggctgc ggcattgccc gtgatgcggt ggggcggggc ttcaccgttg ggctggccga gatgaacgat ctggcaagcg gcacctcttc gcgcgcaacc aagctcattc acggtggttt gcgctatctg gaacattatg aattccggct ggtgtgcgag gcgctgatgg agcgcgaggt gctgtgggcc aatgcgccgc atatcatcca tccgatgcgt ttcgtgctgc cttatcataa gggcggccta cgtcccgcct ggctgttgcg gcttggcctg tttctttacg accatctggg cggccgcaag aagcttcccg ccacgcgcac gctcaacatg cgcaccgaca aggccggcga accgttgaaa ccgcttttca ccaaggcttt tgaatattcc gattgctggg tggacgatgc gcgcttcgtg gcgctcaccg cccgcgatgc ggctgatcgc ggcgcgaaga tcgcgacccg aacgagcgtc gttgccgcaa gccgcgacgg gcagggctgg accatcacac tggaagatac cggcaccggc aggcgtgaga acgtacacgc ccgccttctg gtcaaagccg ccgggccgtg ggccgacaag gttctgcaag gtgtggaagg cgaccggcaa ttgcacaata tccgtctggt gcagggcagc catatcgtcg tgcgcaggaa gttttccgac ccgcgcgcct atttcttcca gaacaacgac gggcgcatca tcttcgccat tccttatgaa gatgatttca ccctgattgg caccacggat caggattata agggcgaccc ggccaaggtg gcgatcaccg acagcgagac cgattatctc tgccaggcgg caagcgaata tttccgcgag ccggtgcgcc gggaggatat cgtctggacc tattccggcg tgcgcccgct ctatgatgac ggggccagca aggcgcagga ggcaacccgc gactatgtgc tgaaggaaga tgcgccagat ggtctggcgc cgctcatcaa tgttttcggc ggcaagctca ccacagcacg caagcttgcc gaacatatgc tgcagaaaat cgagcaccgg cttggccgca agggcgcccc atggacccat gtagcaccat tgccaggcgg ggatttcgag gatgtggctt ttgaaacgga actgaagaag cttgaggcag cctatccttt ccttgatgcg cgccatgcgc gccgcctgtt ccggctctat ggcacccggg catataagct tttggggcag gcttcttcgc tgggcgatct tggccgtcat tttggcagcg atctctatga ggcggaagtg cgctatcttg tcgaaaatga atgggcgcga agcgcggaag atattttgtg gcggcgcacc aagctcggcc tgcgcctgac ggcggcggaa gtcgcggccg tgcagggctt tgtggagcct gcaatcgccg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat