BrcaA.17516.a

PUTATIVE BINDING PROTEIN YDDS PRECURSOR

CENTER ID: BrcaA.17516.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.17516.a.B2.GE38309 Mature protein( BrcaA.17516.a ) 33 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_3321

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLHLGTFVRP ASRRAIISSV LLAIAFLPVT AVAQDSSVFV YADAGEPTTL DPARTNTNYE FTVTRSVYDR LINYDLDDPS SLQPGLAVEW TQDGNAWIVK LREGVKFHDG TSFDAEDVKA TLDRLLKLKL GQSYLVSEIT GVTVIDPATV RIETAQPNVF LPANLSKIEM LSSDDIAAHG GEGDTFFAEG GNGTGPYKLV NWKRGVQIEL ERNTDWWGKF SDKPFDRIVD RFVEDGANRA RGVEGGEFDM ANFIPLDDDP HCRSAGLQAD RRKQSVGLAS HLSQYSKGSH RQCRFPQGAR KGFRLSGHAR FLTWQSHDAT RPHTRLVPLE PRSAGTRNCH QS
NT Sequence
ATGCTACATC TGGGAACATT CGTCCGTCCT GCATCTCGCC GGGCCATCAT CTCATCGGTC TTGCTCGCAA TCGCATTCCT GCCTGTCACC GCAGTGGCGC AAGACAGTTC TGTCTTCGTT TATGCCGACG CCGGTGAACC CACGACACTG GACCCGGCCC GTACCAATAC CAACTATGAG TTCACGGTCA CGCGCAGTGT TTATGACCGG TTGATCAACT ATGATCTCGA CGACCCTTCC AGCCTGCAGC CAGGGCTAGC CGTGGAATGG ACACAGGACG GGAATGCATG GATTGTCAAA CTGCGCGAGG GGGTCAAGTT TCATGATGGC ACCTCATTCG ATGCGGAAGA CGTGAAGGCA ACTCTCGACC GTTTGCTGAA GCTCAAGCTG GGGCAATCCT ATCTGGTTTC CGAAATCACC GGTGTCACTG TGATCGACCC CGCTACCGTG CGGATCGAAA CCGCTCAACC CAATGTCTTC CTGCCTGCGA ATCTGTCGAA GATCGAAATG CTTTCATCCG ATGATATCGC GGCACATGGC GGCGAAGGCG ACACCTTCTT TGCCGAAGGA GGAAACGGCA CGGGGCCTTA TAAGCTCGTA AACTGGAAGC GCGGTGTTCA AATAGAGCTG GAACGTAACA CCGACTGGTG GGGTAAATTC TCTGATAAGC CCTTTGATCG GATCGTCGAC CGCTTCGTGG AAGATGGAGC CAACCGCGCG CGCGGCGTGG AAGGCGGCGA GTTCGATATG GCTAACTTCA TTCCCCTGGA CGATGATCCG CATTGCCGCT CAGCCGGGCT TCAAGCTGAT CGAAGGAAAC AATCTGTGGG CCTGGCCAGC CATCTATCTC AATACTCAAA AGGCTCCCAC AGACAATGCC GATTTCCGCA AGGCGCTCGT AAAGGCTTTC GATTATCAGG CCATGCTCGA TTTCTCACAT GGCAAAGCCA CGACGCCACG CGGCCCCATA CCCGATTGGT TCCTCTTGAG CCCCGAAGCG CAGGAACCCG AAATTGTCAC CAATCT
Details for BrcaA.17516.a.B2.GE38309
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 114
Validated AA Sequence
MAHHHHHHMA QDSSVFVYAD AGEPTTLDPA RTNTNYEFTV TRSVYDRLIN YDLDDPSSLQ PGLAVEWTQD GNAWIVKLRE GVKFHDGTSF DAEDVKATLD RLLKLKLGQS YLVSEITGVT VIDPATVRIE TAQPNVFLPA NLSKIEMLSS DDIAAHGGEG DTFFAEGGNG TGPYKLVNWK RGVQIELERN TDWWGKFSDK PFDRIVDRFV EDGANRARGV EGGEFDMANF IPLDDDPHCR SAGLQADRRK QSVGLASHLS QYSKGSHRQC RFPQGARKGF RLSXHARFLT WQSHDATRPH TRLXPLEPRS AEPEMSPILS EXIGSXXXXP PXRXXXXXXK KXXXXXSXXA TRXXXQXXXL XXSXX
Validated NT Sequence
cnnnnnnnnn cnntanntat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aagacagttc tgtcttcgtt tatgccgacg ccggtgaacc cacgacactg gacccggccc gtaccaatac caactatgag ttcacggtca cgcgcagtgt ttatgaccgg ttgatcaact atgatctcga cgacccttcc agcctgcagc cagggctagc cgtggaatgg acacaggacg ggaatgcatg gattgtcaaa ctgcgcgagg gggtcaagtt tcatgatggc acctcattcg atgcggaaga cgtgaaggca actctcgacc gtttgctgaa gctcaagctg gggcaatcct atctggtttc cgaaatcacc ggtgtcactg tgatcgaccc cgctaccgtg cggatcgaaa ccgctcaacc caatgtcttc ctgcctgcga atctgtcgaa gatcgaaatg ctttcatccg atgatatcgc ggcacatggc ggcgaaggcg acaccttctt tgccgaagga ggaaacggca cggggcctta taagctcgta aactggaagc gcggtgttca aatagagctg gaacgtaaca ccgactggtg gggtaaattc tctgataagc cctttgatcg gatcgtcgac cgcttcgtgg aagatggagc caaccgcgcg cgcggcgtgg aaggcggcga gttcgatatg gctaacttca ttcccctgga cgatgatccg cattgccgct cagccgggct tcaagctgat cgaaggaaac aatctgtggg cctggccagc catctatctc aatactcaaa aggctcccac agacaatgcc gatttccgca aggcgctcgt aaaggctttc gattatcang ccatgctcga tttctcacat ggcaaagcca cgacgccacg cggccccata cccgattggn tcctcttgag ccccgaagcg cagaacccga aatgtcacca atcttaagtg agtanatagg atcgnngnnn nnnnnncccc cnnngagaga nnnnnnnnnn nnnnnaaaaa aannnncgan tncnnnntcn nnnnnngcca cccgnngnnc nnnccaagnc nncnanttgn ctnngtcnng ncnnt
Expected Protein Sequence
MAHHHHHHAQ DSSVFVYADA GEPTTLDPAR TNTNYEFTVT RSVYDRLINY DLDDPSSLQP GLAVEWTQDG NAWIVKLREG VKFHDGTSFD AEDVKATLDR LLKLKLGQSY LVSEITGVTV IDPATVRIET AQPNVFLPAN LSKIEMLSSD DIAAHGGEGD TFFAEGGNGT GPYKLVNWKR GVQIELERNT DWWGKFSDKP FDRIVDRFVE DGANRARGVE GGEFDMANFI PLDDDPHCRS AGLQADRRKQ SVGLASHLSQ YSKGSHRQCR FPQGARKGFR LSGHARFLTW QSHDATRPHT RLVPLEPRSA GTRNCHQS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgcaag acagttctgt cttcgtttat gccgacgccg gtgaacccac gacactggac ccggcccgta ccaataccaa ctatgagttc acggtcacgc gcagtgttta tgaccggttg atcaactatg atctcgacga cccttccagc ctgcagccag ggctagccgt ggaatggaca caggacggga atgcatggat tgtcaaactg cgcgaggggg tcaagtttca tgatggcacc tcattcgatg cggaagacgt gaaggcaact ctcgaccgtt tgctgaagct caagctgggg caatcctatc tggtttccga aatcaccggt gtcactgtga tcgaccccgc taccgtgcgg atcgaaaccg ctcaacccaa tgtcttcctg cctgcgaatc tgtcgaagat cgaaatgctt tcatccgatg atatcgcggc acatggcggc gaaggcgaca ccttctttgc cgaaggagga aacggcacgg ggccttataa gctcgtaaac tggaagcgcg gtgttcaaat agagctggaa cgtaacaccg actggtgggg taaattctct gataagccct ttgatcggat cgtcgaccgc ttcgtggaag atggagccaa ccgcgcgcgc ggcgtggaag gcggcgagtt cgatatggct aacttcattc ccctggacga tgatccgcat tgccgctcag ccgggcttca agctgatcga aggaaacaat ctgtgggcct ggccagccat ctatctcaat actcaaaagg ctcccacaga caatgccgat ttccgcaagg cgctcgtaaa ggctttcgat tatcaggcca tgctcgattt ctcacatggc aaagccacga cgccacgcgg ccccataccc gattggttcc tcttgagccc cgaagcgcag gaacccgaaa ttgtcaccaa tcttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat