BrcaA.17356.a

ABC transporter, periplasmic substrate-binding protein

CENTER ID: BrcaA.17356.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.17356.a.B1.GE38307 Full length( BrcaA.17356.a ) 1 575
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_2795
BV-BRC: fig|483179.4.peg.2795
RefSeq: YP_001594524.1
UniProt: A9MBL6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIEGNP IRWEKLYDFP AVEQDKVIKD TFEKGTPADM LGFVFNTRRP IFADRRVRQA LGLLFDFEWA NRNLFAGQYR RTQSFWEGSQ LSSVGRPADA RERELLAPFP GAVREDVMNG TWHPPVTDGS GHDRVPAKKA YDLLSQAGFQ FKDGMAIDPT GKPFAFEIMT RSPDEEKIAL AYQRNLSRLG IAVEIHTVDD AQYQQRLQTF DYDMILGALA SSLSPGNEQW LRWGSASRDV QGSFNFAGVA DPAVDAMIEA LLAARNRADF VSAVRALDRV LISGDYYVPL YHLPYQWVAR WDRIEHPQKT PLSGYQLPAW WHTSQ
NT Sequence
ATGCATGGCG ACGTGGCTTT GCCTGCCGAT TATACCCATT TTCCCTACAC CAATCCCGAT GCCCCGAAAA AGGGTTCGCT GACCGTAGGC GTGGTGGGCA CTTTCGACAG CCTTAATCCC TTTGTGCTGA AAAGTATGCG CACCACCGCG CGCGGCCTGT ATAATGATGG CGAGTTTGGC AATATGGTGT ACCAGACGCT GATGCTGCGC TCGCGCGATG AGCCTTTTAC GCTCTATAGT CTGCTGGCTG AGAAAGTTGC CATCGATCCC GAGCGCAAAT GGGTGGAATT CACGCTCAAT CCAAAAGCCA AATGGTCAGA TGGCCAGCCT GTCACGGTGG ACGATGTTCT CTTCACCTAT GACATACTGA CCGAAAAGGG CCGCCCGCCC TATAACAGCC GCATGAGCCG GGTGGCGAAG ATCGAAAAGA CCGGCGAACG CTCGGTGCGC TTCACATTCA ACGAGAAATC CGACCGTGAG TTTCCGATGC TGATCGCAGG CTCGATGCCG GTTCTGCCGA AACATGCCAT CAACCGCGAT ACGTTCGGCA ATTCCACACT GGAACCGCCG ATCGGCAGCG GCCCCTATGT GGTCGCCAGT GTGCAGCCCG GCCAGCGGAT CGTCTACAAG CGCAACCCGG ACTATTGGGG CAAGGATCTG CCCTCGCAGC GCGGCTTCAA CAATTTCGAC AAGATCTCCA TCGAATATTA CCGCAACGAA ACCTCGCTGT TTGAATCCTT CAAAAAGGGC ATTCTCGACA TTTTCATTGA AGGCAATCCG ATACGCTGGG AAAAGCTCTA TGATTTTCCG GCGGTCGAGC AGGACAAGGT CATCAAGGAC ACTTTCGAGA AGGGCACACC GGCCGATATG CTGGGCTTCG TGTTCAATAC GCGCCGCCCG ATCTTCGCTG ACCGGCGTGT GCGCCAGGCC CTTGGATTGC TGTTCGATTT TGAGTGGGCG AACCGCAATC TCTTTGCAGG GCAATATCGC CGTACGCAGA GCTTCTGGGA GGGGTCGCAA CTTTCATCCG TCGGCAGGCC CGCCGATGCG CGCGAAAGGG AGTTGCTGGC CCCCTTCCCC GGTGCTGTGC GCGAAGACGT GATGAATGGC ACCTGGCACC CGCCCGTTAC AGATGGCAGC GGCCATGACC GCGTTCCGGC CAAGAAGGCC TATGATCTTC TAAGCCAGGC CGGTTTCCAG TTCAAGGACG GCATGGCCAT AGATCCGACC GGTAAACCGT TTGCCTTCGA GATTATGACG CGATCACCGG ACGAGGAGAA AATCGCGCTC GCCTATCAGC GCAACCTCTC GCGCCTTGGC ATAGCAGTTG AAATCCACAC GGTGGACGAT GCGCAATATC AACAGCGCCT GCAAACCTTC GATTACGATA TGATCCTCGG TGCACTTGCC AGTTCGCTTT CTCCCGGCAA TGAGCAATGG TTGCGGTGGG GTTCCGCCTC GCGCGATGTG CAGGGCAGCT TCAACTTTGC GGGCGTGGCC GACCCTGCCG TTGATGCGAT GATCGAGGCG CTGCTTGCCG CGCGCAACCG CGCCGATTTC GTCAGCGCCG TGCGTGCACT CGACCGTGTG CTGATTTCAG GTGATTATTA TGTTCCGCTC TACCACCTGC CCTATCAATG GGTTGCGCGG TGGGACCGGA TTGAACATCC CCAAAAAACA CCACTTTCCG GCTACCAACT GCCGGCATGG TGGCACACGT CCCAA
Details for BrcaA.17356.a.B1.GE38307
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 66kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 45
Validated AA Sequence
MHGDVALPAD YTHFPYTNPD APKKGSLTVG VVGTFDSLNP FVLKSMRTTA RGLYNDGEFG NMVYQTLMLR SRDEPFTLYS LLAEKVAIDP ERKWVEFTLN PKAKWSDGQP VTVDDVLFTY DILTEKGRPP YNSRMSRVAK IEKTGERSVR FTFNEKSDRE FPMLIAGSMP VLPKHAINRD TFGNSTLEPP IGSGPYVVAS VQPGQRIVYK RNPDYWGKDL PSQRGFNNFD KISIEYYRNE TSLFESFKKG ILDIFIERQS DTLGKAL
Validated NT Sequence
cnnnnnggnn nncncnannn taantttgtt taactttaag aaggagatat accagtggct caccaccacc accaccatat gcatggcgac gtggctttgc ctgccgatta tacccatttt ccctacacca atcccgatgc cccgaaaaag ggttcgctga ccgtaggcgt ggtgggcact ttcgacagcc ttaatccctt tgtgctgaaa agtatgcgca ccaccgcgcg cggcctgtat aatgatggcg agtttggcaa tatggtgtac cagacgctga tgctgcgctc gcgcgatgag ccttttacgc tctatagtct gctggctgag aaagttgcca tcgatcccga gcgcaaatgg gtggaattca cgctcaatcc aaaagccaaa tggtcagatg gccagcctgt cacggtggac gatgttctct tcacctatga catactgacc gaaaagggcc gcccgcccta taacagccgc atgagccggg tggcgaagat cgaaaagacc ggcgaacgct cggtgcgctt cacattcaac gagaaatccg accgtgagtt tccgatgctg atcgcaggct cgatgccggt tctgccgaaa catgccatca accgcgatac gttcggcaat tccacactgg aaccgccgat cggcagcggc ccctatgtgg tcgccagtgt gcagcccggc cagcggatcg tctacaagcg caacccggac tattggggca aggatctgcc ctcgcagcgc ggcttcaaca atttcgacaa gatctccatc gaatattacc gcaacgaaac ctcgctgttt gaatccttca aaaagggcat tctcgacatt ttcattgaaa ggcaatccga tacgctggga aaagctctat gattttccgg cggtcgagca ggacaaggtc atcaaggaca ctttcgagaa gggcacaccg gccgatatgc tgggcttcgt gttcaatacg cgccgcccga tcttcgctga ccgggcgtgt gcgccngncc ttngattgct gttcgatttt gagtgggcga acncaatctc tttgcagggc aatatcnnnt acgcagagct tctgggaggg gncgcanntt catcgtcgnn nnngncngat gcnnncnnnn nnnngctgnc cnntncccgn ctgtgcgcna anannnanna ntgnnnnnnn nnnnnnacnn atgcannnac cnnnnnnnnn nnnnaanng
Expected Protein Sequence
MAHHHHHHMH GDVALPADYT HFPYTNPDAP KKGSLTVGVV GTFDSLNPFV LKSMRTTARG LYNDGEFGNM VYQTLMLRSR DEPFTLYSLL AEKVAIDPER KWVEFTLNPK AKWSDGQPVT VDDVLFTYDI LTEKGRPPYN SRMSRVAKIE KTGERSVRFT FNEKSDREFP MLIAGSMPVL PKHAINRDTF GNSTLEPPIG SGPYVVASVQ PGQRIVYKRN PDYWGKDLPS QRGFNNFDKI SIEYYRNETS LFESFKKGIL DIFIEGNPIR WEKLYDFPAV EQDKVIKDTF EKGTPADMLG FVFNTRRPIF ADRRVRQALG LLFDFEWANR NLFAGQYRRT QSFWEGSQLS SVGRPADARE RELLAPFPGA VREDVMNGTW HPPVTDGSGH DRVPAKKAYD LLSQAGFQFK DGMAIDPTGK PFAFEIMTRS PDEEKIALAY QRNLSRLGIA VEIHTVDDAQ YQQRLQTFDY DMILGALASS LSPGNEQWLR WGSASRDVQG SFNFAGVADP AVDAMIEALL AARNRADFVS AVRALDRVLI SGDYYVPLYH LPYQWVARWD RIEHPQKTPL SGYQLPAWWH TSQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg gcgacgtggc tttgcctgcc gattataccc attttcccta caccaatccc gatgccccga aaaagggttc gctgaccgta ggcgtggtgg gcactttcga cagccttaat ccctttgtgc tgaaaagtat gcgcaccacc gcgcgcggcc tgtataatga tggcgagttt ggcaatatgg tgtaccagac gctgatgctg cgctcgcgcg atgagccttt tacgctctat agtctgctgg ctgagaaagt tgccatcgat cccgagcgca aatgggtgga attcacgctc aatccaaaag ccaaatggtc agatggccag cctgtcacgg tggacgatgt tctcttcacc tatgacatac tgaccgaaaa gggccgcccg ccctataaca gccgcatgag ccgggtggcg aagatcgaaa agaccggcga acgctcggtg cgcttcacat tcaacgagaa atccgaccgt gagtttccga tgctgatcgc aggctcgatg ccggttctgc cgaaacatgc catcaaccgc gatacgttcg gcaattccac actggaaccg ccgatcggca gcggccccta tgtggtcgcc agtgtgcagc ccggccagcg gatcgtctac aagcgcaacc cggactattg gggcaaggat ctgccctcgc agcgcggctt caacaatttc gacaagatct ccatcgaata ttaccgcaac gaaacctcgc tgtttgaatc cttcaaaaag ggcattctcg acattttcat tgaaggcaat ccgatacgct gggaaaagct ctatgatttt ccggcggtcg agcaggacaa ggtcatcaag gacactttcg agaagggcac accggccgat atgctgggct tcgtgttcaa tacgcgccgc ccgatcttcg ctgaccggcg tgtgcgccag gcccttggat tgctgttcga ttttgagtgg gcgaaccgca atctctttgc agggcaatat cgccgtacgc agagcttctg ggaggggtcg caactttcat ccgtcggcag gcccgccgat gcgcgcgaaa gggagttgct ggcccccttc cccggtgctg tgcgcgaaga cgtgatgaat ggcacctggc acccgcccgt tacagatggc agcggccatg accgcgttcc ggccaagaag gcctatgatc ttctaagcca ggccggtttc cagttcaagg acggcatggc catagatccg accggtaaac cgtttgcctt cgagattatg acgcgatcac cggacgagga gaaaatcgcg ctcgcctatc agcgcaacct ctcgcgcctt ggcatagcag ttgaaatcca cacggtggac gatgcgcaat atcaacagcg cctgcaaacc ttcgattacg atatgatcct cggtgcactt gccagttcgc tttctcccgg caatgagcaa tggttgcggt ggggttccgc ctcgcgcgat gtgcagggca gcttcaactt tgcgggcgtg gccgaccctg ccgttgatgc gatgatcgag gcgctgcttg ccgcgcgcaa ccgcgccgat ttcgtcagcg ccgtgcgtgc actcgaccgt gtgctgattt caggtgatta ttatgttccg ctctaccacc tgccctatca atgggttgcg cggtgggacc ggattgaaca tccccaaaaa acaccacttt ccggctacca actgccggca tggtggcaca cgtcccaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat