BrcaA.17302.a

Mobile element protein

CENTER ID: BrcaA.17302.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.17302.a.B1.GE38306 Full length( BrcaA.17302.a ) 1 153
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_0554
BV-BRC: fig|483179.4.peg.554
RefSeq: YP_001592383.1
RefSeq: YP_001592394.1
UniProt: A9M948

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIQSFEIGEP LMTRRRYELT DHEWSIISPL LPNKPRGVAR VDDRRVLNGI LWRFRTGSPW AEVPERYGPP TACYNRFVRW RKAGVWDRLL EAVSKAYDGD IVMIDSTCVR VHQHAATGKK GMETMVAWDV PAAGLRAKST RSLMPKVARS IYV
NT Sequence
ATGATTCAAA GCTTCGAAAT AGGGGAGCCT TTGATGACGC GTCGTCGCTA CGAACTAACT GACCACGAAT GGTCGATTAT ATCGCCGTTG TTGCCCAACA AACCACGCGG CGTTGCTCGC GTTGATGATC GGCGGGTGCT GAATGGCATC CTGTGGCGAT TTCGAACGGG CTCGCCCTGG GCGGAAGTTC CCGAACGCTA TGGCCCGCCG ACGGCCTGTT ACAACCGCTT CGTGCGCTGG CGCAAAGCCG GGGTCTGGGA TCGGCTTCTT GAAGCGGTGT CCAAAGCCTA CGATGGCGAC ATCGTCATGA TCGACTCGAC CTGTGTCCGC GTTCACCAGC ACGCGGCCAC AGGAAAAAAG GGGATGGAGA CGATGGTGGC ATGGGACGTT CCCGCGGCGG GCTTACGAGC AAAATCCACG CGCTCGTTGA TGCCGAAGGT CGCCCGGTCA ATCTACGTC
Details for BrcaA.17302.a.B1.GE38306
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 19kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI QSFEIGEPLM TRRRYELTDH EWSIISPLLP NKPRGVARVD DRRVLNGILW RFRTGSPWAE VPERYGPPTA CYNRFVRWRK AGVWDRLLEA VSKAYDGDIV MIDSTCVRVH QHAATGKKGM ETMVAWDVPA AGLRAKSTRS LMPKVARSIY V
Validated NT Sequence
cnntaganta nttttgttta ntttaagaag gagatatacc atggctcacc accaccacca ccatatgatt caaagcttcg aaatagggga gcctttgatg acgcgtcgtc gctacgaact aactgaccac gaatggtcga ttatatcgcc gttgttgccc aacaaaccac gcggcgttgc tcgcgttgat gatcggcggg tgctgaatgg catcctgtgg cgatttcgaa cgggctcgcc ctgggcggaa gttcccgaac gctatggccc gccgacggcc tgttacaacc gcttcgtgcg ctggcgcaaa gccggggtct gggatcggct tcttgaagcg gtgtccaaag cctacgatgg cgacatcgtc atgatcgact cgacctgtgt ccgcgttcac cagcacgcgg ccacaggaaa aaaggggatg gagacgatgg tggcatggga cgttcccgcg gcgggcttac gagcaaaatc cacgcgctcg ttgatgccga aggtcgcccg gtcaatctac gtctaagtga gtaagatagg atccggctgc taacaagccc ccgaaagann nggtgctnnt ngccatcggt cgcgttcagc cgattgaaac ggttgccgag gctacaaggc tcctgcgtga ggtgggtatc aaccgcatca atttctacct gatctatggt ctgccttttg cagacggacg agacattgcg cgaaacctgc gagtgcnttg tgacactctc accgnatcag gttgcctgct atggctatgc ncatctgccg cagcgccgtg caaatcancg tctcatcgat gaaaacacgc tgcctgacnc ggacnagcgt ttccntcngc ccgcatcgta ncgacagctt cnntggtttc gctatcagcc ggttggtatc gaccattttg cgctnccnna tgacancttg cnatcactgc ncgcgaaagg cnctctgaat cgcaatttcn gggctatncc aacgaccgnt gcggaaccct gatcgggttg cccgtcatcg atctcnnnnn ttcccgggcg gtatncgccn natnnttncn nantntnngg caannnccca agnnnntgnn cccgnnnnct nnnnnnnntt nnngnntnnn nnnccctnnc tgacacnana cnnnnnnccn nnnnnncnac atncncccnn nnctnannng caattctgnn tnnnntnnnn nnnaaaga
Expected Protein Sequence
MAHHHHHHMI QSFEIGEPLM TRRRYELTDH EWSIISPLLP NKPRGVARVD DRRVLNGILW RFRTGSPWAE VPERYGPPTA CYNRFVRWRK AGVWDRLLEA VSKAYDGDIV MIDSTCVRVH QHAATGKKGM ETMVAWDVPA AGLRAKSTRS LMPKVARSIY V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc aaagcttcga aataggggag cctttgatga cgcgtcgtcg ctacgaacta actgaccacg aatggtcgat tatatcgccg ttgttgccca acaaaccacg cggcgttgct cgcgttgatg atcggcgggt gctgaatggc atcctgtggc gatttcgaac gggctcgccc tgggcggaag ttcccgaacg ctatggcccg ccgacggcct gttacaaccg cttcgtgcgc tggcgcaaag ccggggtctg ggatcggctt cttgaagcgg tgtccaaagc ctacgatggc gacatcgtca tgatcgactc gacctgtgtc cgcgttcacc agcacgcggc cacaggaaaa aaggggatgg agacgatggt ggcatgggac gttcccgcgg cgggcttacg agcaaaatcc acgcgctcgt tgatgccgaa ggtcgcccgg tcaatctacg tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat