BrcaA.17298.a

Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22)

CENTER ID: BrcaA.17298.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.17298.a.B1.GE38305 Full length( BrcaA.17298.a ) 1 379
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_0672

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHDDAIRRYA ALAVPRYTSY PTAADFVPVG MDTTRRWLRQ IGPEESVSLY IHVPYCSQIC HYCGCNAKMA IREDVIENFV NALLGEIRTV SASLTARPRV AHLHWGGGTP SILNAGQFQT VLAAIRTAFD FDLSMEHAIE LDPRTVTPLL AKTLAEMGVN RASLGVQDVD SRVQMAIGRV QPIETVAEAT RLLREVGINR INFDLIYGLP LQTVETLRET CERVVTLSPD RVACYGYAHL PQRRANQRLI DENTLPDADE RFRQARIVAD SFIGFGYQPV GIDHFALPDD DLAIAAREGT LNRNFQGYTN DRCGTLIGLA RHRSRSFRAV MRRIFPMSGN IASAWKPASW RRFAATRCVT QTVSAPPSSP RSCAISASI
NT Sequence
ATGCATGACG ACGCGATAAG ACGCTACGCA GCACTGGCTG TTCCTCGTTA TACTTCCTAT CCTACTGCGG CGGATTTCGT GCCGGTCGGC ATGGATACCA CGCGCCGTTG GCTGCGCCAG ATCGGGCCGG AAGAAAGCGT TTCGCTTTAT ATCCATGTGC CGTATTGCAG TCAGATATGC CACTATTGCG GCTGCAACGC AAAAATGGCG ATACGTGAGG ATGTGATCGA AAATTTCGTA AATGCGCTTC TTGGAGAAAT CCGGACTGTG AGCGCAAGCC TTACGGCGCG TCCCCGCGTT GCCCATCTTC ACTGGGGCGG CGGCACACCC TCCATTCTCA ATGCCGGGCA ATTTCAGACT GTTCTGGCCG CAATCCGCAC GGCGTTTGAT TTCGATCTCT CGATGGAACA CGCCATAGAG CTTGATCCAC GCACGGTAAC ACCGCTTTTG GCGAAGACGC TTGCCGAAAT GGGTGTAAAT CGCGCAAGCC TCGGTGTGCA GGATGTGGAC AGCAGGGTGC AAATGGCCAT CGGTCGCGTT CAGCCGATTG AAACGGTTGC CGAGGCTACA AGGCTCCTGC GTGAGGTGGG TATCAACCGC ATCAATTTCG ACCTGATCTA TGGTCTGCCT TTGCAGACGG TCGAGACATT GCGCGAAACC TGCGAGCGCG TTGTGACGCT CTCACCGGAT CGGGTTGCCT GCTATGGCTA TGCGCATCTG CCGCAGCGCC GTGCAAATCA GCGTCTCATC GATGAAAACA CGCTGCCTGA CGCGGACGAG CGTTTCCGTC AGGCCCGCAT CGTAGCGGAC AGCTTCATTG GTTTCGGCTA TCAGCCGGTT GGTATCGACC ATTTTGCGCT GCCGGATGAC GATCTTGCGA TCGCTGCGCG CGAAGGCACT CTGAATCGCA ATTTTCAGGG CTATACCAAC GACCGTTGCG GAACCCTGAT CGGGTTGGCC CGTCATCGAT CTCGCAGTTT CCGGGCGGTT ATGCGCAGAA TATTTCCGAT GTCGGGCAAT ATCGCAAGCG CGTGGAAGCC GGCGAGCTGG CGACGGTTCG CGGCTACACG CTGCGTGACA CAGACCGTAT CCGCTCCGCC ATCATCTCCT CGCTCATGTG CAATTTCTGC GTCGATC
Details for BrcaA.17298.a.B1.GE38305
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 43kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMH DDAIRRYAAL AVPRYTSYPT AADFVPVGMD TTRRWLRQIG PEESVSLYIH VPYCSQICHY CGCNAKMAIR EDVIENFVNA LLGEIRTVSA SLTARPRVAH LHWGGGTPSI LNAGQFQTVL AAIRTAFDFD LSMEHAIELD PRTVTPLLAK TLAEMGVNRA SLGVQDVDSR VQMAIGRVQP IETVAEATRL LREVGINRIN FDLIYGLPLQ TVETLRETCE CVVTLSPDRV ACYGYAHLPQ RRANQRLIDE NTLPDADERF RQARIVADSF IGFGYQPVGI DHFALPDDDL AIAARERHSE SQFSGYTXDR CGTLIGLARH RSRSFRAXMR RXISDVGQYP SXXXPAXWAX XSRLTPAXXX XXXPXXXHXX XVXXXXXXXX XRXXX
Validated NT Sequence
gnnanncctc tagaanattt tgttnaactt taagaaggag atataccatg gctcaccacc accaccacca tatgcatgac gacgcgataa gacgctacgc agcactggct gttcctcgtt atacttccta tcctactgcg gcggatttcg tgccggtcgg catggatacc acgcgccgtt ggctgcgcca gatcgggccg gaagaaagcg tttcgcttta tatccatgtg ccgtattgca gtcagatatg ccactattgc ggctgcaacg caaaaatggc gatacgtgag gatgtgatcg aaaatttcgt aaatgcgctt cttggagaaa tccggactgt gagcgcaagc cttacggcgc gtccccgcgt tgcccatctt cactggggcg gcggcacacc ctccattctc aatgccgggc aatttcagac tgttctggcc gcaatccgca cggcgtttga tttcgatctc tcgatggaac acgccataga gcttgatcca cgcacggtaa caccgctttt ggcgaagacg cttgccgaaa tgggtgtaaa tcgcgcaagc ctcggtgtgc aggatgtgga cagcagggtg caaatggcca tcggtcgcgt tcagccgatt gaaacggttg ccgaggctac aaggctcctg cgtgaggtgg gtatcaaccg catcaatttc gacctgatct atggtctgcc tttgcagacg gtcgagacat tgcgcgaaac ctgcgagtgc gttgtgacgc tctcaccgga tcgggttgcc tgctatggct atgcgcatct gccgcagcgc cgtgcaaatc agcgtctcat cgatgaaaac acgctgcctg acgcggacga gcgtttccgt caggcccgca tcgtagcgga cagcttcatt ggtttcggct atcagccggt tggtatcgac cattttgcgc tgccggatga cgatcttgcg atcgctgcgc gcgaaaggca ctctgaatcg caattttcng gctataccna cgaccgttgc ggaaccctga tcgggttggc ccgtcatcga tctcgcagtt tccgggcggn natgcgcaga aanatttccg atgtngggca atatccaagc nnnnngnnnc cggcnnnctg ggcgannngt tcncggctaa cacctgcnnn gannnanana ncgnatccgc nccnncntca ttntncnnnn gtnnnnnnnn nnnnnnntnn anntaangnt agangacana nna
Expected Protein Sequence
MAHHHHHHMH DDAIRRYAAL AVPRYTSYPT AADFVPVGMD TTRRWLRQIG PEESVSLYIH VPYCSQICHY CGCNAKMAIR EDVIENFVNA LLGEIRTVSA SLTARPRVAH LHWGGGTPSI LNAGQFQTVL AAIRTAFDFD LSMEHAIELD PRTVTPLLAK TLAEMGVNRA SLGVQDVDSR VQMAIGRVQP IETVAEATRL LREVGINRIN FDLIYGLPLQ TVETLRETCE RVVTLSPDRV ACYGYAHLPQ RRANQRLIDE NTLPDADERF RQARIVADSF IGFGYQPVGI DHFALPDDDL AIAAREGTLN RNFQGYTNDR CGTLIGLARH RSRSFRAVMR RIFPMSGNIA SAWKPASWRR FAATRCVTQT VSAPPSSPRS CAISASI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg acgacgcgat aagacgctac gcagcactgg ctgttcctcg ttatacttcc tatcctactg cggcggattt cgtgccggtc ggcatggata ccacgcgccg ttggctgcgc cagatcgggc cggaagaaag cgtttcgctt tatatccatg tgccgtattg cagtcagata tgccactatt gcggctgcaa cgcaaaaatg gcgatacgtg aggatgtgat cgaaaatttc gtaaatgcgc ttcttggaga aatccggact gtgagcgcaa gccttacggc gcgtccccgc gttgcccatc ttcactgggg cggcggcaca ccctccattc tcaatgccgg gcaatttcag actgttctgg ccgcaatccg cacggcgttt gatttcgatc tctcgatgga acacgccata gagcttgatc cacgcacggt aacaccgctt ttggcgaaga cgcttgccga aatgggtgta aatcgcgcaa gcctcggtgt gcaggatgtg gacagcaggg tgcaaatggc catcggtcgc gttcagccga ttgaaacggt tgccgaggct acaaggctcc tgcgtgaggt gggtatcaac cgcatcaatt tcgacctgat ctatggtctg cctttgcaga cggtcgagac attgcgcgaa acctgcgagc gcgttgtgac gctctcaccg gatcgggttg cctgctatgg ctatgcgcat ctgccgcagc gccgtgcaaa tcagcgtctc atcgatgaaa acacgctgcc tgacgcggac gagcgtttcc gtcaggcccg catcgtagcg gacagcttca ttggtttcgg ctatcagccg gttggtatcg accattttgc gctgccggat gacgatcttg cgatcgctgc gcgcgaaggc actctgaatc gcaattttca gggctatacc aacgaccgtt gcggaaccct gatcgggttg gcccgtcatc gatctcgcag tttccgggcg gttatgcgca gaatatttcc gatgtcgggc aatatcgcaa gcgcgtggaa gccggcgagc tggcgacggt tcgcggctac acgctgcgtg acacagaccg tatccgctcc gccatcatct cctcgctcat gtgcaatttc tgcgtcgatc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat