BrcaA.00111.a

glutamine synthetase family protein

CENTER ID: BrcaA.00111.a
ORGANISM: Brucella canis ATCC 23365
ASSOCIATED DISEASE: Brucellosis in canids
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrcaA.00111.a.B1.GE38299 Full length( BrcaA.00111.a ) 1 455
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruCan25663_2945
BV-BRC: fig|483179.4.peg.2945
RefSeq: YP_001594677.1
UniProt: A9MC19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGQLTFDEL KKAVAEGRID TVLACIVDMQ GRLIGKRFYG QFFVESGYDE THGCNYLLAD DIDMEPVPGY EAAGWDKGYG DFVMKPDLST LRLAPWLEKT AIVLCDVLDH HHDDLSHSPR AVLKKQVQRL HERGYRAYFA SELEFYIFDE TYKSARAKRW HEMETASPYV QDYVIHLTTR EEPVLRAMRN HLADAGIPVE NSKGEWGPGQ QELNVRYCKA LEMADRHVIM KNAMKEIAEA HGKCITFMAK YDYARAGSSS HVHNSIWSAD GKEPLFFDPK APYTMTPLMR SWVAGQIKYA TDYTYFLAPY INSYKRFQAG TFAPTKIMWS QDNRTAGFRL CGEGTKGIRI KCRIGGADIN PYLAFAALIA AGLKGVDEKL ELDEPFVGDA YSAVRLKEIP YTLREAAAAL KGSAFLKEAF GEDVVNHYTH TAHWEQIEYD RRVTDWELYR GFERY
NT Sequence
ATGGCCGGAC AGTTGACTTT TGACGAATTG AAGAAGGCAG TCGCGGAAGG CAGGATCGAC ACGGTGCTGG CCTGCATCGT GGACATGCAG GGCCGCCTGA TTGGAAAGCG CTTCTACGGA CAGTTTTTCG TGGAATCCGG TTATGACGAA ACCCACGGCT GCAATTATCT TCTGGCCGAC GATATCGATA TGGAACCCGT ACCGGGCTAT GAGGCGGCGG GTTGGGACAA AGGCTATGGC GATTTCGTCA TGAAGCCGGA TCTCTCCACG CTGCGCCTTG CGCCGTGGCT GGAAAAGACG GCCATTGTGC TGTGCGATGT ACTGGACCAC CATCATGACG ATCTTTCCCA TTCGCCGCGC GCCGTCCTGA AAAAACAGGT GCAGCGCCTG CACGAGCGCG GTTATCGCGC TTATTTCGCA TCCGAGCTTG AATTCTACAT TTTCGATGAA ACCTATAAAT CGGCGCGGGC AAAACGCTGG CATGAGATGG AAACCGCCTC GCCTTACGTG CAGGATTATG TGATCCACCT CACCACCAGG GAGGAGCCGG TCCTGCGCGC CATGCGCAAC CATCTGGCCG ATGCGGGCAT TCCGGTGGAA AATTCAAAAG GCGAATGGGG GCCGGGCCAG CAGGAGCTGA ACGTGCGCTA TTGCAAGGCG CTTGAAATGG CCGATCGCCA CGTCATCATG AAAAACGCGA TGAAGGAGAT TGCCGAGGCG CATGGCAAGT GCATCACCTT CATGGCGAAG TATGATTATG CAAGGGCTGG CAGTTCGAGC CATGTGCACA ATTCCATCTG GAGTGCAGAT GGCAAGGAAC CTCTGTTCTT CGATCCCAAA GCCCCCTACA CCATGACACC GCTGATGCGC TCATGGGTGG CCGGCCAGAT CAAATATGCG ACCGATTACA CCTATTTTCT GGCGCCTTAC ATCAATTCCT ACAAGCGGTT TCAGGCGGGC ACCTTCGCCC CCACCAAGAT CATGTGGAGC CAGGACAACC GCACAGCCGG TTTTCGCCTT TGCGGCGAAG GTACCAAGGG CATTCGCATC AAATGCCGCA TCGGCGGCGC GGATATCAAT CCCTATCTGG CCTTTGCAGC GCTCATCGCC GCCGGCCTGA AAGGCGTGGA TGAAAAGCTG GAACTGGACG AACCTTTTGT GGGCGATGCC TATAGCGCGG TGAGGCTGAA GGAAATTCCT TACACGCTGC GCGAGGCGGC TGCGGCGCTC AAAGGTTCCG CTTTCCTGAA AGAAGCCTTC GGCGAGGATG TCGTGAACCA TTACACCCAT ACCGCCCATT GGGAGCAGAT CGAATATGAC CGCCGCGTGA CCGACTGGGA GCTCTACCGC GGATTTGAAC GCTAC
Details for BrcaA.00111.a.B1.GE38299
HARVESTED ON: 7/21/2014
SEQUENCED ON: 7/26/2014
EXPECTED MW: 53kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMA GQLTFDELKK AVAEGRIDTV LACIVDMQGR LIGKRFYGQF FVESGYDETH GCNYLLADDI DMEPVPGYEA AGWDKGYGDF VMKPDLSTLR LAPWLEKTAI VLCDVLDHHH DDLSHSPRAV LKKQVQRLHE RGYRAYFASE LEFYIFDETY KSARAKRWHE METASPYVQD YVIHLTTREE PVLRAMRNHL ADAGIPVENS KGEWGPGQQE LNVRYCKALE MADRHVIMKN AMKEIAEAHG KCITFMAKYD YARAGSSSHV HNSIWSADGK EPLFFDPKAP YTMTPLMRSW VGRPDXYATD YTYFLALHHS YXGFXRAXRP XXHVXXHXTX XRFAXXXGIA SXXIXXXSXP XCXHXXXXXX XXXXXXXXXX X
Validated NT Sequence
cnctagnnta ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggcc ggacagttga cttttgacga attgaagaag gcagtcgcgg aaggcaggat cgacacggtg ctggcctgca tcgtggacat gcagggccgc ctgattggaa agcgcttcta cggacagttt ttcgtggaat ccggttatga cgaaacccac ggctgcaatt atcttctggc cgacgatatc gatatggaac ccgtaccggg ctatgaggcg gcgggttggg acaaaggcta tggcgatttc gtcatgaagc cggatctctc cacgctgcgc cttgcgccgt ggctggaaaa gacggccatt gtgctgtgcg atgtactgga ccaccatcat gacgatcttt cccattcgcc gcgcgccgtc ctgaaaaaac aggtgcagcg cctgcacgag cgcggttatc gcgcttattt cgcatccgag cttgaattct acattttcga tgaaacctat aaatcggcgc gggcaaaacg ctggcatgag atggaaaccg cctcgcctta cgtgcaggat tatgtgatcc acctcaccac cagggaggag ccggtcctgc gcgccatgcg caaccatctg gccgatgcgg gcattccggt ggaaaattca aaaggcgaat gggggccggg ccagcaggag ctgaacgtgc gctattgcaa ggcgcttgaa atggccgatc gccacgtcat catgaaaaac gcgatgaagg agattgccga ggcgcatggc aagtgcatca ccttcatggc gaagtatgat tatgcaaggg ctggcagttc gagccatgtg cacaattcca tctggagtgc agatggcaag gaacctctgt tcttcgatcc caaagccccc tacaccatga caccgctgat gcgctcatgg gtgggccggc cagatcanta tgcgaccgat tacacctatt ttctggcgct acatcattcc tacancggtt tcnggcgggc acnncgcccc annnatcatg tgnncagnca cngcacannn nttcgctttg cgnnannacn nggcatcgca tcaangcnca tcgnnncnnn ntcnatnccn ntctgcnnnc atncnnnnnn nnnnnnnnnn nnnntnnncn gacnannnng ngnnnannnc nntnnc
Expected Protein Sequence
MAHHHHHHMA GQLTFDELKK AVAEGRIDTV LACIVDMQGR LIGKRFYGQF FVESGYDETH GCNYLLADDI DMEPVPGYEA AGWDKGYGDF VMKPDLSTLR LAPWLEKTAI VLCDVLDHHH DDLSHSPRAV LKKQVQRLHE RGYRAYFASE LEFYIFDETY KSARAKRWHE METASPYVQD YVIHLTTREE PVLRAMRNHL ADAGIPVENS KGEWGPGQQE LNVRYCKALE MADRHVIMKN AMKEIAEAHG KCITFMAKYD YARAGSSSHV HNSIWSADGK EPLFFDPKAP YTMTPLMRSW VAGQIKYATD YTYFLAPYIN SYKRFQAGTF APTKIMWSQD NRTAGFRLCG EGTKGIRIKC RIGGADINPY LAFAALIAAG LKGVDEKLEL DEPFVGDAYS AVRLKEIPYT LREAAAALKG SAFLKEAFGE DVVNHYTHTA HWEQIEYDRR VTDWELYRGF ERY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccg gacagttgac ttttgacgaa ttgaagaagg cagtcgcgga aggcaggatc gacacggtgc tggcctgcat cgtggacatg cagggccgcc tgattggaaa gcgcttctac ggacagtttt tcgtggaatc cggttatgac gaaacccacg gctgcaatta tcttctggcc gacgatatcg atatggaacc cgtaccgggc tatgaggcgg cgggttggga caaaggctat ggcgatttcg tcatgaagcc ggatctctcc acgctgcgcc ttgcgccgtg gctggaaaag acggccattg tgctgtgcga tgtactggac caccatcatg acgatctttc ccattcgccg cgcgccgtcc tgaaaaaaca ggtgcagcgc ctgcacgagc gcggttatcg cgcttatttc gcatccgagc ttgaattcta cattttcgat gaaacctata aatcggcgcg ggcaaaacgc tggcatgaga tggaaaccgc ctcgccttac gtgcaggatt atgtgatcca cctcaccacc agggaggagc cggtcctgcg cgccatgcgc aaccatctgg ccgatgcggg cattccggtg gaaaattcaa aaggcgaatg ggggccgggc cagcaggagc tgaacgtgcg ctattgcaag gcgcttgaaa tggccgatcg ccacgtcatc atgaaaaacg cgatgaagga gattgccgag gcgcatggca agtgcatcac cttcatggcg aagtatgatt atgcaagggc tggcagttcg agccatgtgc acaattccat ctggagtgca gatggcaagg aacctctgtt cttcgatccc aaagccccct acaccatgac accgctgatg cgctcatggg tggccggcca gatcaaatat gcgaccgatt acacctattt tctggcgcct tacatcaatt cctacaagcg gtttcaggcg ggcaccttcg cccccaccaa gatcatgtgg agccaggaca accgcacagc cggttttcgc ctttgcggcg aaggtaccaa gggcattcgc atcaaatgcc gcatcggcgg cgcggatatc aatccctatc tggcctttgc agcgctcatc gccgccggcc tgaaaggcgt ggatgaaaag ctggaactgg acgaaccttt tgtgggcgat gcctatagcg cggtgaggct gaaggaaatt ccttacacgc tgcgcgaggc ggctgcggcg ctcaaaggtt ccgctttcct gaaagaagcc ttcggcgagg atgtcgtgaa ccattacacc cataccgccc attgggagca gatcgaatat gaccgccgcg tgaccgactg ggagctctac cgcggatttg aacgctactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat