BrcaA.00010.a
FolM Alternative dihydrofolate reductase 1
| CENTER ID: | BrcaA.00010.a |
| ORGANISM: | Brucella canis ATCC 23365 |
| ASSOCIATED DISEASE: | Brucellosis in canids |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BrcaA.00010.a.B1.GE38297 | Full length( BrcaA.00010.a ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5BT9
DEPOSITED: 6/2/2015
DETERMINATION: XRay
CLONE: BrcaA.00010.a.B1.GE38297
PROTEIN: BrcaA.00010.a.B1.PS02349
Publications by SSGCID
Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis
Dranow DM, Edwards TE, Myler PJ, Staker BL, Subramanian S, Smith C, Mayclin SJ, Asojo OA, Porter I, Neal T, Hayes D, Thedford M, Fowler K, Billups N, Rhoades A, Smith K, Walker Z
Acta Crystallogr F Struct Biol Commun - 2021
volume 78, issue Pt 1, pages 31-38
PMID: 34981773; PMCID: PMC8725004
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | PATRIC_Locus_Tag:VBIBruCan25663_0717 |
| BV-BRC: | fig|483179.4.peg.717 |
| RefSeq: | YP_001592556.1 |
| UniProt: | A9MA73 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLNDPEARMV ANCPVLVTGG ARRIGKAIVE DLASHGFPVA IHCNRSLDEG EAIANRINDS GGNACVVQAD LEGDVRGLVK QASDRIGPIR LLVNNASLFQ EDKVGALDMA LWDRHFAVHL KTPVILAEDM RKALPEDQDG LVVNIIDQRV WKLNPQFFSY TLSKTALWNA TRTLAQALAP RIRVNAIAPG PTLPSERQRP EDFERQVSKL PLQRAPELPE FGRTVRYFWE NRSITGQMIA LDGGQHLAWE TPDIAGITE
NT Sequence
GTGTTGAATG ATCCCGAAGC CCGCATGGTT GCAAATTGCC CCGTCCTCGT GACAGGCGGC GCACGGCGCA TTGGGAAGGC GATCGTCGAA GACCTCGCCA GCCATGGTTT TCCGGTTGCC ATTCATTGCA ATCGCTCGCT TGATGAAGGC GAGGCAATTG CAAATCGCAT CAACGACAGT GGCGGCAATG CCTGCGTGGT TCAAGCCGAC CTTGAAGGCG ATGTGCGTGG TTTGGTGAAA CAGGCCTCGG ACCGGATCGG CCCCATTCGC CTGCTCGTCA ACAATGCTTC CCTGTTTCAG GAGGATAAGG TCGGCGCCCT TGATATGGCC TTGTGGGACC GGCATTTCGC CGTTCACCTG AAAACGCCTG TGATTCTGGC AGAAGACATG CGCAAAGCCC TGCCGGAAGA TCAGGACGGG CTGGTGGTCA ATATCATCGA CCAGCGCGTG TGGAAGCTCA ATCCGCAGTT CTTTTCCTAT ACGCTGTCCA AAACGGCTTT GTGGAACGCG ACGCGCACGC TGGCGCAGGC TTTGGCGCCA CGCATCCGCG TCAACGCCAT CGCGCCCGGC CCCACCTTGC CCAGCGAGCG CCAGCGGCCG GAAGATTTCG AGCGGCAGGT TAGCAAACTT CCCCTCCAGC GCGCACCGGA ATTGCCGGAA TTCGGGCGTA CGGTTCGCTA TTTCTGGGAA AACCGTTCCA TTACCGGCCA GATGATTGCG CTCGACGGCG GGCAGCACCT GGCATGGGAA ACGCCCGACA TTGCAGGAAT TACCGAA
Details for BrcaA.00010.a.B1.GE38297
| HARVESTED ON: | 7/21/2014 |
| SEQUENCED ON: | 7/26/2014 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV LNDPEARMVA NCPVLVTGGA RRIGKAIVED LASHGFPVAI HCNRSLDEGE AIANRINDSG GNACVVQADL EGDVRGLVKQ ASDRIGPIRL LVNNASLFQE DKVGALDMAL WDRHFAVHLK TPVILAEDMR KALPEDQDGL VVNIIDQRVW KLNPQFFSYT LSKTALWNAT RTLAQALAPR IRVNAIAPGP TLPSERQRPE DFERQVSKLP LQRAPELPEF GRTVRYFWEN RSITGQMIAL DGGQHLAWET PDIAELPNK
Validated NT Sequence
annnncncta gaatattttg tttactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgttgaat gatcccgaag cccgcatggt tgcaaattgc cccgtcctcg tgacaggcgg cgcacggcgc attgggaagg cgatcgtcga agacctcgcc agccatggtt ttccggttgc cattcattgc aatcgctcgc ttgatgaagg cgaggcaatt gcaaatcgca tcaacgacag tggcggcaat gcctgcgtgg ttcaagccga ccttgaaggc gatgtgcgtg gtttggtgaa acaggcctcg gaccggatcg gccccattcg cctgctcgtc aacaatgctt ccctgtttca ggaggataag gtcggcgccc ttgatatggc cttgtgggac cggcatttcg ccgttcacct gaaaacgcct gtgattctgg cagaagacat gcgcaaagcc ctgccggaag atcaggacgg gctggtggtc aatatcatcg accagcgcgt gtggaagctc aatccgcagt tcttttccta tacgctgtcc aaaacggctt tgtggaacgc gacgcgcacg ctggcgcagg ctttggcgcc acgcatccgc gtcaacgcca tcgcgcccgg ccccaccttg cccagcgagc gccagcggcc ggaagatttc gagcggcagg ttagcaaact tcccctccag cgcgcaccgg aattgccgga attcgggcgt acggttcgct atttctggga aaaccgttcc attaccggcc agatgattgc gctcgacggc gggcagcacc tggcatggga aacgcccgac attgcagaat taccgaataa gtgagtaaga taggatccgg ctgctacana ccccccnaag anannnnncg cnaananann nnnanatccn tncnnncttc aactccctag atgcttacng gcngtagant tgtaccgaag acgnnaagna nggncagcta
Expected Protein Sequence
MAHHHHHHML NDPEARMVAN CPVLVTGGAR RIGKAIVEDL ASHGFPVAIH CNRSLDEGEA IANRINDSGG NACVVQADLE GDVRGLVKQA SDRIGPIRLL VNNASLFQED KVGALDMALW DRHFAVHLKT PVILAEDMRK ALPEDQDGLV VNIIDQRVWK LNPQFFSYTL SKTALWNATR TLAQALAPRI RVNAIAPGPT LPSERQRPED FERQVSKLPL QRAPELPEFG RTVRYFWENR SITGQMIALD GGQHLAWETP DIAGITE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgttga atgatcccga agcccgcatg gttgcaaatt gccccgtcct cgtgacaggc ggcgcacggc gcattgggaa ggcgatcgtc gaagacctcg ccagccatgg ttttccggtt gccattcatt gcaatcgctc gcttgatgaa ggcgaggcaa ttgcaaatcg catcaacgac agtggcggca atgcctgcgt ggttcaagcc gaccttgaag gcgatgtgcg tggtttggtg aaacaggcct cggaccggat cggccccatt cgcctgctcg tcaacaatgc ttccctgttt caggaggata aggtcggcgc ccttgatatg gccttgtggg accggcattt cgccgttcac ctgaaaacgc ctgtgattct ggcagaagac atgcgcaaag ccctgccgga agatcaggac gggctggtgg tcaatatcat cgaccagcgc gtgtggaagc tcaatccgca gttcttttcc tatacgctgt ccaaaacggc tttgtggaac gcgacgcgca cgctggcgca ggctttggcg ccacgcatcc gcgtcaacgc catcgcgccc ggccccacct tgcccagcga gcgccagcgg ccggaagatt tcgagcggca ggttagcaaa cttcccctcc agcgcgcacc ggaattgccg gaattcgggc gtacggttcg ctatttctgg gaaaaccgtt ccattaccgg ccagatgatt gcgctcgacg gcgggcagca cctggcatgg gaaacgcccg acattgcagg aattaccgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat